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P36401

- E13H_TOBAC

UniProt

P36401 - E13H_TOBAC

Protein

Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q'

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Implicated in the defense of plants against pathogens.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei262 – 2621NucleophileBy similarity
    Active sitei320 – 3201Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase
    Short name:
    (1->3)-beta-glucanase
    Beta-1,3-endoglucanase
    PR-35
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 339315Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q'PRO_0000011878Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei25 – 251Pyrrolidone carboxylic acidCurated

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Pyrrolidone carboxylic acid

    Expressioni

    Inductioni

    Accumulates following infection.

    Structurei

    3D structure databases

    ProteinModelPortaliP36401.
    SMRiP36401. Positions 26-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHLIVTLLL LSVLTLATLD FTGAQAGVCY GRQGNGLPSP ADVVSLCNRN    50
    NIRRMRIYDP DQPTLEALRG SNIELMLGVP NPDLENVAAS QANADTWVQN 100
    NVRNYGNVKF RYIAVGNEVS PLNENSKYVP VLLNAMRNIQ TAISGAGLGN 150
    QIKVSTAIET GLTTDTSPPS NGRFKDDVRQ FIEPIINFLV TNRAPLLVNL 200
    YPYFAIANNA DIKLEYALFT SSEVVVNDNG RGYRNLFDAI LDATYSALEK 250
    ASGSSLEIVV SESGWPSAGA GQLTSIDNAR TYNNNLISHV KGGSPKRPSG 300
    PIETYVFALF DEDQKDPEIE KHFGLFSANM QPKYQISFN 339
    Length:339
    Mass (Da):36,995
    Last modified:June 1, 1994 - v1
    Checksum:iFDFA0D4F7300235E
    GO

    Sequence cautioni

    The sequence CAA38324.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti297 – 2971R → A AA sequence (PubMed:16594025)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54456 mRNA. Translation: CAA38324.1. Different initiation.
    PIRiS12402.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54456 mRNA. Translation: CAA38324.1 . Different initiation.
    PIRi S12402.

    3D structure databases

    ProteinModelPortali P36401.
    SMRi P36401. Positions 26-338.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evidence for a third structural class of beta-1,3-glucanase in tobacco."
      Payne G., Ward E., Gaffney T., Ahl Goy P., Moyer M., Harper A., Meins F. Jr., Ryals J.
      Plant Mol. Biol. 15:797-808(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. Xanthi.
    2. "Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
      van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
      Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 57-69; 112-126; 281-291; 297-321 AND 333-339.

    Entry informationi

    Entry nameiE13H_TOBAC
    AccessioniPrimary (citable) accession number: P36401
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3