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P36401 (E13H_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q'

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase
PR-35
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the defense of plants against pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Secretedextracellular spaceapoplast.

Induction

Accumulates following infection.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Sequence caution

The sequence CAA38324.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMPyrrolidone carboxylic acid
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 339315Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q'
PRO_0000011878

Sites

Active site2621Nucleophile By similarity
Active site3201Proton donor By similarity

Amino acid modifications

Modified residue251Pyrrolidone carboxylic acid Probable

Experimental info

Sequence conflict2971R → A AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P36401 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: FDFA0D4F7300235E

FASTA33936,995
        10         20         30         40         50         60 
MAHLIVTLLL LSVLTLATLD FTGAQAGVCY GRQGNGLPSP ADVVSLCNRN NIRRMRIYDP 

        70         80         90        100        110        120 
DQPTLEALRG SNIELMLGVP NPDLENVAAS QANADTWVQN NVRNYGNVKF RYIAVGNEVS 

       130        140        150        160        170        180 
PLNENSKYVP VLLNAMRNIQ TAISGAGLGN QIKVSTAIET GLTTDTSPPS NGRFKDDVRQ 

       190        200        210        220        230        240 
FIEPIINFLV TNRAPLLVNL YPYFAIANNA DIKLEYALFT SSEVVVNDNG RGYRNLFDAI 

       250        260        270        280        290        300 
LDATYSALEK ASGSSLEIVV SESGWPSAGA GQLTSIDNAR TYNNNLISHV KGGSPKRPSG 

       310        320        330 
PIETYVFALF DEDQKDPEIE KHFGLFSANM QPKYQISFN 

« Hide

References

[1]"Evidence for a third structural class of beta-1,3-glucanase in tobacco."
Payne G., Ward E., Gaffney T., Ahl Goy P., Moyer M., Harper A., Meins F. Jr., Ryals J.
Plant Mol. Biol. 15:797-808(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Xanthi.
[2]"Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 57-69; 112-126; 281-291; 297-321 AND 333-339.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54456 mRNA. Translation: CAA38324.1. Different initiation.
PIRS12402.

3D structure databases

ProteinModelPortalP36401.
SMRP36401. Positions 26-338.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13H_TOBAC
AccessionPrimary (citable) accession number: P36401
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries