Reviewed, UniProtKB/Swiss-Prot P36401 (E13H_TOBAC)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q'     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase       Short name=(1->3)-beta-glucanase     Beta-1,3-endoglucanase     PR-35
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space › apoplast.
Induction	Accumulates following infection.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 339	315	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q'		PRO_0000011878
Sites
	Active site	262	1	Nucleophile By similarity
	Active site	320	1	Proton donor By similarity
Amino acid modifications
	Modified residue	25	1	Pyrrolidone carboxylic acid Probable
Experimental info
	Sequence conflict	297	1	R → A AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P36401-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: FDFA0D4F7300235E	FASTA	339	36,995
	10 20 30 40 50 60 MAHLIVTLLL LSVLTLATLD FTGAQAGVCY GRQGNGLPSP ADVVSLCNRN NIRRMRIYDP 70 80 90 100 110 120 DQPTLEALRG SNIELMLGVP NPDLENVAAS QANADTWVQN NVRNYGNVKF RYIAVGNEVS 130 140 150 160 170 180 PLNENSKYVP VLLNAMRNIQ TAISGAGLGN QIKVSTAIET GLTTDTSPPS NGRFKDDVRQ 190 200 210 220 230 240 FIEPIINFLV TNRAPLLVNL YPYFAIANNA DIKLEYALFT SSEVVVNDNG RGYRNLFDAI 250 260 270 280 290 300 LDATYSALEK ASGSSLEIVV SESGWPSAGA GQLTSIDNAR TYNNNLISHV KGGSPKRPSG 310 320 330 PIETYVFALF DEDQKDPEIE KHFGLFSANM QPKYQISFN

References

[1]	"Evidence for a third structural class of beta-1,3-glucanase in tobacco." Payne G., Ward E., Gaffney T., Ahl Goy P., Moyer M., Harper A., Meins F. Jr., Ryals J. Plant Mol. Biol. 15:797-808(1990) [PubMed: 2103473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Xanthi.
[2]	"Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system." van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J. Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed: 16594025] [Abstract] Cited for: PROTEIN SEQUENCE OF 57-69; 112-126; 281-291; 297-321 AND 333-339.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54456 mRNA. Translation: CAA38324.1. Different initiation.
PIR	S12402.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 298.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13H_TOBAC

Accession

Primary (citable) accession number: P36401

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 22, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)