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P36397 (ARF1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ADP-ribosylation factor 1

Short name=AtARF1
Gene names
Name:ARF1
Ordered Locus Names:At2g47170
ORF Names:T3D7.2, T8I13.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes. Ref.7 Ref.8 Ref.9 Ref.11 Ref.14

Enzyme regulation

Activated by AGD7 and AGD10. Ref.12 Ref.13

Subunit structure

Interacts with AGD7 and GDAP1. GDP-locked form interacts with cytosolic tail of p24 proteins. Ref.10 Ref.13 Ref.14

Subcellular location

Golgi apparatus. Endosome Ref.6 Ref.11.

Sequence similarities

Belongs to the small GTPase superfamily. Arf family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Potential
Chain2 – 181180ADP-ribosylation factor 1
PRO_0000207426

Regions

Nucleotide binding24 – 318GTP By similarity
Nucleotide binding67 – 715GTP By similarity
Nucleotide binding126 – 1294GTP By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine Potential

Experimental info

Mutagenesis311T → N: Constitutively inactive form (GDP-locked form); loss of intracellular protein trafficking. Ref.7 Ref.8 Ref.9
Mutagenesis711Q → L: Constitutively active form (GTP-locked form). Ref.8 Ref.9

Secondary structure

.................................. 181
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36397 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 7EDDEC87B7B29592

FASTA18120,609
        10         20         30         40         50         60 
MGLSFGKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN 

        70         80         90        100        110        120 
ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV VEARDELHRM LNEDELRDAV 

       130        140        150        160        170        180 
LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ RHWYIQSTCA TSGEGLYEGL DWLSNNIASK 


A 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family."
Regad F., Bardet C., Tremousaygue D., Moisan A., Lescure B., Axelos M.
FEBS Lett. 316:133-136(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"In situ localization and in vitro induction of plant COPI-coated vesicles."
Pimpl P., Movafeghi A., Coughlan S., Denecke J., Hillmer S., Robinson D.G.
Plant Cell 12:2219-2236(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis."
Lee M.H., Min M.K., Lee Y.J., Jin J.B., Shin D.H., Kim D.H., Lee K.-H., Hwang I.
Plant Physiol. 129:1507-1520(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF THR-31, FUNCTION.
[8]"Arf1 GTPase plays roles in the protein traffic between the endoplasmic reticulum and the Golgi apparatus in tobacco and Arabidopsis cultured cells."
Takeuchi M., Ueda T., Yahara N., Nakano A.
Plant J. 31:499-515(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
[9]"The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole."
Pimpl P., Hanton S.L., Taylor J.P., Pinto-daSilva L.L., Denecke J.
Plant Cell 15:1242-1256(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
[10]"Sorting signals in the cytosolic tail of membrane proteins involved in the interaction with plant ARF1 and coatomer."
Contreras I., Ortiz-Zapater E., Aniento F.
Plant J. 38:685-698(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH P24 PROTEINS.
[11]"Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity."
Xu J., Scheres B.
Plant Cell 17:525-536(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[12]"RPA, a class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis."
Song X.-F., Yang C.-Y., Liu J., Yang W.-C.
Plant Physiol. 141:966-976(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[13]"Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells."
Min M.K., Kim S.J., Miao Y., Shin J., Jiang L., Hwang I.
Plant Physiol. 143:1601-1614(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AGD7, ENZYME REGULATION.
[14]"Multiple roles of ADP-ribosylation factor 1 in plant cells include spatially regulated recruitment of coatomer and elements of the Golgi matrix."
Matheson L.A., Hanton S.L., Rossi M., Latijnhouwers M., Stefano G., Renna L., Brandizzi F.
Plant Physiol. 143:1615-1627(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GDAP1, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95166 mRNA. Translation: AAA32729.1.
AC002337 Genomic DNA. Translation: AAB63817.1.
AC007236 Genomic DNA. Translation: AAM15469.1.
CP002685 Genomic DNA. Translation: AEC10810.1.
AY074859 mRNA. Translation: AAL75910.1.
AY142032 mRNA. Translation: AAM98296.1.
AY087342 mRNA. Translation: AAM64892.1.
PIRS28875.
RefSeqNP_182239.1. NM_130285.2.
UniGeneAt.12215.
At.23262.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3AQ4X-ray1.80A/B1-181[»]
ProteinModelPortalP36397.
SMRP36397. Positions 4-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4665. 1 interaction.
IntActP36397. 1 interaction.
STRING3702.AT2G47170.1-P.

Proteomic databases

PRIDEP36397.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G47170.1; AT2G47170.1; AT2G47170.
GeneID819330.
KEGGath:AT2G47170.

Organism-specific databases

TAIRAT2G47170.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000163691.
InParanoidP36397.
KOK07937.
OMAHYFRNTE.
PhylomeDBP36397.

Gene expression databases

GenevestigatorP36397.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamPF00025. Arf. 1 hit.
[Graphical view]
PRINTSPR00328. SAR1GTPBP.
SMARTSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameARF1_ARATH
AccessionPrimary (citable) accession number: P36397
Secondary accession number(s): Q7G9L3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names