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P36397

- ARF1_ARATH

UniProt

P36397 - ARF1_ARATH

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Protein

ADP-ribosylation factor 1

Gene
ARF1, At2g47170, T3D7.2, T8I13.1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes.5 Publications

Enzyme regulationi

Activated by AGD7 and AGD10.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP By similarity
Nucleotide bindingi67 – 715GTP By similarity
Nucleotide bindingi126 – 1294GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: TAIR
  2. phospholipase activator activity Source: TAIR

GO - Biological processi

  1. positive regulation of catalytic activity Source: GOC
  2. protein transport Source: UniProtKB-KW
  3. small GTPase mediated signal transduction Source: InterPro
  4. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Short name:
AtARF1
Gene namesi
Name:ARF1
Ordered Locus Names:At2g47170
ORF Names:T3D7.2, T8I13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G47170.

Subcellular locationi

Golgi apparatus. Endosome 2 Publications

GO - Cellular componenti

  1. endosome Source: UniProtKB-SubCell
  2. Golgi apparatus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311T → N: Constitutively inactive form (GDP-locked form); loss of intracellular protein trafficking. 3 Publications
Mutagenesisi71 – 711Q → L: Constitutively active form (GTP-locked form). 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Reviewed prediction
Chaini2 – 181180ADP-ribosylation factor 1PRO_0000207426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine Reviewed prediction

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PRIDEiP36397.

Expressioni

Gene expression databases

GenevestigatoriP36397.

Interactioni

Subunit structurei

Interacts with AGD7 and GDAP1. GDP-locked form interacts with cytosolic tail of p24 proteins.3 Publications

Protein-protein interaction databases

BioGridi4665. 1 interaction.
IntActiP36397. 1 interaction.
STRINGi3702.AT2G47170.1-P.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 95
Helixi10 – 123
Beta strandi17 – 259
Helixi30 – 378
Beta strandi42 – 487
Beta strandi51 – 588
Beta strandi61 – 688
Helixi79 – 846
Beta strandi85 – 939
Helixi97 – 993
Helixi100 – 11112
Helixi114 – 1163
Beta strandi120 – 1267
Helixi136 – 1427
Helixi145 – 1473
Beta strandi153 – 1575
Turni160 – 1634
Helixi166 – 17813

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AQ4X-ray1.80A/B1-181[»]
ProteinModelPortaliP36397.
SMRiP36397. Positions 4-180.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000163691.
InParanoidiP36397.
KOiK07937.
OMAiHYFRNTE.
PhylomeDBiP36397.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36397-1 [UniParc]FASTAAdd to Basket

« Hide

MGLSFGKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG    50
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV 100
VEARDELHRM LNEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ 150
RHWYIQSTCA TSGEGLYEGL DWLSNNIASK A 181
Length:181
Mass (Da):20,609
Last modified:January 23, 2007 - v2
Checksum:i7EDDEC87B7B29592
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95166 mRNA. Translation: AAA32729.1.
AC002337 Genomic DNA. Translation: AAB63817.1.
AC007236 Genomic DNA. Translation: AAM15469.1.
CP002685 Genomic DNA. Translation: AEC10810.1.
AY074859 mRNA. Translation: AAL75910.1.
AY142032 mRNA. Translation: AAM98296.1.
AY087342 mRNA. Translation: AAM64892.1.
PIRiS28875.
RefSeqiNP_182239.1. NM_130285.2.
UniGeneiAt.12215.
At.23262.

Genome annotation databases

EnsemblPlantsiAT2G47170.1; AT2G47170.1; AT2G47170.
GeneIDi819330.
KEGGiath:AT2G47170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95166 mRNA. Translation: AAA32729.1 .
AC002337 Genomic DNA. Translation: AAB63817.1 .
AC007236 Genomic DNA. Translation: AAM15469.1 .
CP002685 Genomic DNA. Translation: AEC10810.1 .
AY074859 mRNA. Translation: AAL75910.1 .
AY142032 mRNA. Translation: AAM98296.1 .
AY087342 mRNA. Translation: AAM64892.1 .
PIRi S28875.
RefSeqi NP_182239.1. NM_130285.2.
UniGenei At.12215.
At.23262.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AQ4 X-ray 1.80 A/B 1-181 [» ]
ProteinModelPortali P36397.
SMRi P36397. Positions 4-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4665. 1 interaction.
IntActi P36397. 1 interaction.
STRINGi 3702.AT2G47170.1-P.

Proteomic databases

PRIDEi P36397.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G47170.1 ; AT2G47170.1 ; AT2G47170 .
GeneIDi 819330.
KEGGi ath:AT2G47170.

Organism-specific databases

TAIRi AT2G47170.

Phylogenomic databases

eggNOGi COG1100.
HOGENOMi HOG000163691.
InParanoidi P36397.
KOi K07937.
OMAi HYFRNTE.
PhylomeDBi P36397.

Gene expression databases

Genevestigatori P36397.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family."
    Regad F., Bardet C., Tremousaygue D., Moisan A., Lescure B., Axelos M.
    FEBS Lett. 316:133-136(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "In situ localization and in vitro induction of plant COPI-coated vesicles."
    Pimpl P., Movafeghi A., Coughlan S., Denecke J., Hillmer S., Robinson D.G.
    Plant Cell 12:2219-2236(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis."
    Lee M.H., Min M.K., Lee Y.J., Jin J.B., Shin D.H., Kim D.H., Lee K.-H., Hwang I.
    Plant Physiol. 129:1507-1520(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-31, FUNCTION.
  8. "Arf1 GTPase plays roles in the protein traffic between the endoplasmic reticulum and the Golgi apparatus in tobacco and Arabidopsis cultured cells."
    Takeuchi M., Ueda T., Yahara N., Nakano A.
    Plant J. 31:499-515(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
  9. "The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole."
    Pimpl P., Hanton S.L., Taylor J.P., Pinto-daSilva L.L., Denecke J.
    Plant Cell 15:1242-1256(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
  10. "Sorting signals in the cytosolic tail of membrane proteins involved in the interaction with plant ARF1 and coatomer."
    Contreras I., Ortiz-Zapater E., Aniento F.
    Plant J. 38:685-698(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH P24 PROTEINS.
  11. "Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity."
    Xu J., Scheres B.
    Plant Cell 17:525-536(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "RPA, a class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis."
    Song X.-F., Yang C.-Y., Liu J., Yang W.-C.
    Plant Physiol. 141:966-976(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  13. "Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells."
    Min M.K., Kim S.J., Miao Y., Shin J., Jiang L., Hwang I.
    Plant Physiol. 143:1601-1614(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AGD7, ENZYME REGULATION.
  14. "Multiple roles of ADP-ribosylation factor 1 in plant cells include spatially regulated recruitment of coatomer and elements of the Golgi matrix."
    Matheson L.A., Hanton S.L., Rossi M., Latijnhouwers M., Stefano G., Renna L., Brandizzi F.
    Plant Physiol. 143:1615-1627(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GDAP1, FUNCTION.

Entry informationi

Entry nameiARF1_ARATH
AccessioniPrimary (citable) accession number: P36397
Secondary accession number(s): Q7G9L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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