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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes.5 Publications

Enzyme regulationi

Activated by AGD7 and AGD10.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTP binding Source: TAIR
  • phospholipase activator activity Source: TAIR

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-1660514 Synthesis of PIPs at the Golgi membrane
R-ATH-421837 Clathrin derived vesicle budding
R-ATH-6807878 COPI-mediated anterograde transport
R-ATH-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Short name:
AtARF1
Gene namesi
Name:ARF1
Ordered Locus Names:At2g47170
ORF Names:T3D7.2, T8I13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47170
TAIRilocus:2065114 AT2G47170

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → N: Constitutively inactive form (GDP-locked form); loss of intracellular protein trafficking. 3 Publications1
Mutagenesisi71Q → L: Constitutively active form (GTP-locked form). 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074262 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP36397
PRIDEiP36397

PTM databases

iPTMnetiP36397

Expressioni

Gene expression databases

ExpressionAtlasiP36397 baseline and differential
GenevisibleiP36397 AT

Interactioni

Subunit structurei

Interacts with AGD7 and GDAP1. GDP-locked form interacts with cytosolic tail of p24 proteins.3 Publications

Protein-protein interaction databases

BioGridi4665, 1 interactor
IntActiP36397, 1 interactor
MINTiP36397
STRINGi3702.AT2G47170.1

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 9Combined sources5
Helixi10 – 12Combined sources3
Beta strandi17 – 25Combined sources9
Helixi30 – 37Combined sources8
Beta strandi42 – 48Combined sources7
Beta strandi51 – 58Combined sources8
Beta strandi61 – 68Combined sources8
Helixi79 – 84Combined sources6
Beta strandi85 – 93Combined sources9
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 178Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AQ4X-ray1.80A/B1-181[»]
ProteinModelPortaliP36397
SMRiP36397
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
HOGENOMiHOG000163691
KOiK07937
OMAiAMREMAN
OrthoDBiEOG09360NRP
PhylomeDBiP36397

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSFGKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV
110 120 130 140 150
VEARDELHRM LNEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ
160 170 180
RHWYIQSTCA TSGEGLYEGL DWLSNNIASK A
Length:181
Mass (Da):20,609
Last modified:January 23, 2007 - v2
Checksum:i7EDDEC87B7B29592
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95166 mRNA Translation: AAA32729.1
AC002337 Genomic DNA Translation: AAB63817.1
AC007236 Genomic DNA Translation: AAM15469.1
CP002685 Genomic DNA Translation: AEC10810.1
CP002685 Genomic DNA Translation: ANM62132.1
CP002685 Genomic DNA Translation: ANM62133.1
AY074859 mRNA Translation: AAL75910.1
AY142032 mRNA Translation: AAM98296.1
AY087342 mRNA Translation: AAM64892.1
PIRiS28875
RefSeqiNP_001324310.1, NM_001337250.1
NP_001324311.1, NM_001337251.1
NP_182239.1, NM_130285.3
UniGeneiAt.12215
At.23262

Genome annotation databases

EnsemblPlantsiAT2G47170.1; AT2G47170.1; AT2G47170
AT2G47170.2; AT2G47170.2; AT2G47170
AT2G47170.3; AT2G47170.3; AT2G47170
GeneIDi819330
GrameneiAT2G47170.1; AT2G47170.1; AT2G47170
AT2G47170.2; AT2G47170.2; AT2G47170
AT2G47170.3; AT2G47170.3; AT2G47170
KEGGiath:AT2G47170

Similar proteinsi

Entry informationi

Entry nameiARF1_ARATH
AccessioniPrimary (citable) accession number: P36397
Secondary accession number(s): Q7G9L3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health