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P36397

- ARF1_ARATH

UniProt

P36397 - ARF1_ARATH

Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes.5 Publications

    Enzyme regulationi

    Activated by AGD7 and AGD10.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi24 – 318GTPBy similarity
    Nucleotide bindingi67 – 715GTPBy similarity
    Nucleotide bindingi126 – 1294GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: TAIR
    2. phospholipase activator activity Source: TAIR

    GO - Biological processi

    1. positive regulation of catalytic activity Source: GOC
    2. protein transport Source: UniProtKB-KW
    3. small GTPase mediated signal transduction Source: InterPro
    4. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor 1
    Short name:
    AtARF1
    Gene namesi
    Name:ARF1
    Ordered Locus Names:At2g47170
    ORF Names:T3D7.2, T8I13.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G47170.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome Source: UniProtKB-SubCell
    2. Golgi apparatus Source: TAIR

    Keywords - Cellular componenti

    Endosome, Golgi apparatus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi31 – 311T → N: Constitutively inactive form (GDP-locked form); loss of intracellular protein trafficking. 3 Publications
    Mutagenesisi71 – 711Q → L: Constitutively active form (GTP-locked form). 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 181180ADP-ribosylation factor 1PRO_0000207426Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    PRIDEiP36397.

    Expressioni

    Gene expression databases

    GenevestigatoriP36397.

    Interactioni

    Subunit structurei

    Interacts with AGD7 and GDAP1. GDP-locked form interacts with cytosolic tail of p24 proteins.3 Publications

    Protein-protein interaction databases

    BioGridi4665. 1 interaction.
    IntActiP36397. 1 interaction.
    STRINGi3702.AT2G47170.1-P.

    Structurei

    Secondary structure

    1
    181
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 95
    Helixi10 – 123
    Beta strandi17 – 259
    Helixi30 – 378
    Beta strandi42 – 487
    Beta strandi51 – 588
    Beta strandi61 – 688
    Helixi79 – 846
    Beta strandi85 – 939
    Helixi97 – 993
    Helixi100 – 11112
    Helixi114 – 1163
    Beta strandi120 – 1267
    Helixi136 – 1427
    Helixi145 – 1473
    Beta strandi153 – 1575
    Turni160 – 1634
    Helixi166 – 17813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AQ4X-ray1.80A/B1-181[»]
    ProteinModelPortaliP36397.
    SMRiP36397. Positions 4-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Arf family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000163691.
    InParanoidiP36397.
    KOiK07937.
    OMAiHYFRNTE.
    PhylomeDBiP36397.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view]
    PfamiPF00025. Arf. 1 hit.
    [Graphical view]
    PRINTSiPR00328. SAR1GTPBP.
    SMARTiSM00177. ARF. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51417. ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36397-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLSFGKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG    50
    FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV 100
    VEARDELHRM LNEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ 150
    RHWYIQSTCA TSGEGLYEGL DWLSNNIASK A 181
    Length:181
    Mass (Da):20,609
    Last modified:January 23, 2007 - v2
    Checksum:i7EDDEC87B7B29592
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95166 mRNA. Translation: AAA32729.1.
    AC002337 Genomic DNA. Translation: AAB63817.1.
    AC007236 Genomic DNA. Translation: AAM15469.1.
    CP002685 Genomic DNA. Translation: AEC10810.1.
    AY074859 mRNA. Translation: AAL75910.1.
    AY142032 mRNA. Translation: AAM98296.1.
    AY087342 mRNA. Translation: AAM64892.1.
    PIRiS28875.
    RefSeqiNP_182239.1. NM_130285.2.
    UniGeneiAt.12215.
    At.23262.

    Genome annotation databases

    EnsemblPlantsiAT2G47170.1; AT2G47170.1; AT2G47170.
    GeneIDi819330.
    KEGGiath:AT2G47170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95166 mRNA. Translation: AAA32729.1 .
    AC002337 Genomic DNA. Translation: AAB63817.1 .
    AC007236 Genomic DNA. Translation: AAM15469.1 .
    CP002685 Genomic DNA. Translation: AEC10810.1 .
    AY074859 mRNA. Translation: AAL75910.1 .
    AY142032 mRNA. Translation: AAM98296.1 .
    AY087342 mRNA. Translation: AAM64892.1 .
    PIRi S28875.
    RefSeqi NP_182239.1. NM_130285.2.
    UniGenei At.12215.
    At.23262.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AQ4 X-ray 1.80 A/B 1-181 [» ]
    ProteinModelPortali P36397.
    SMRi P36397. Positions 4-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4665. 1 interaction.
    IntActi P36397. 1 interaction.
    STRINGi 3702.AT2G47170.1-P.

    Proteomic databases

    PRIDEi P36397.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G47170.1 ; AT2G47170.1 ; AT2G47170 .
    GeneIDi 819330.
    KEGGi ath:AT2G47170.

    Organism-specific databases

    TAIRi AT2G47170.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000163691.
    InParanoidi P36397.
    KOi K07937.
    OMAi HYFRNTE.
    PhylomeDBi P36397.

    Gene expression databases

    Genevestigatori P36397.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view ]
    Pfami PF00025. Arf. 1 hit.
    [Graphical view ]
    PRINTSi PR00328. SAR1GTPBP.
    SMARTi SM00177. ARF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51417. ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family."
      Regad F., Bardet C., Tremousaygue D., Moisan A., Lescure B., Axelos M.
      FEBS Lett. 316:133-136(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "In situ localization and in vitro induction of plant COPI-coated vesicles."
      Pimpl P., Movafeghi A., Coughlan S., Denecke J., Hillmer S., Robinson D.G.
      Plant Cell 12:2219-2236(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis."
      Lee M.H., Min M.K., Lee Y.J., Jin J.B., Shin D.H., Kim D.H., Lee K.-H., Hwang I.
      Plant Physiol. 129:1507-1520(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-31, FUNCTION.
    8. "Arf1 GTPase plays roles in the protein traffic between the endoplasmic reticulum and the Golgi apparatus in tobacco and Arabidopsis cultured cells."
      Takeuchi M., Ueda T., Yahara N., Nakano A.
      Plant J. 31:499-515(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
    9. "The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole."
      Pimpl P., Hanton S.L., Taylor J.P., Pinto-daSilva L.L., Denecke J.
      Plant Cell 15:1242-1256(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
    10. "Sorting signals in the cytosolic tail of membrane proteins involved in the interaction with plant ARF1 and coatomer."
      Contreras I., Ortiz-Zapater E., Aniento F.
      Plant J. 38:685-698(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH P24 PROTEINS.
    11. "Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity."
      Xu J., Scheres B.
      Plant Cell 17:525-536(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "RPA, a class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis."
      Song X.-F., Yang C.-Y., Liu J., Yang W.-C.
      Plant Physiol. 141:966-976(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    13. "Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells."
      Min M.K., Kim S.J., Miao Y., Shin J., Jiang L., Hwang I.
      Plant Physiol. 143:1601-1614(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AGD7, ENZYME REGULATION.
    14. "Multiple roles of ADP-ribosylation factor 1 in plant cells include spatially regulated recruitment of coatomer and elements of the Golgi matrix."
      Matheson L.A., Hanton S.L., Rossi M., Latijnhouwers M., Stefano G., Renna L., Brandizzi F.
      Plant Physiol. 143:1615-1627(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GDAP1, FUNCTION.

    Entry informationi

    Entry nameiARF1_ARATH
    AccessioniPrimary (citable) accession number: P36397
    Secondary accession number(s): Q7G9L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3