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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes.5 Publications

Enzyme regulationi

Activated by AGD7 and AGD10.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTP binding Source: TAIR
  • phospholipase activator activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-1660514. Synthesis of PIPs at the Golgi membrane.
R-ATH-421837. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Short name:
AtARF1
Gene namesi
Name:ARF1
Ordered Locus Names:At2g47170
ORF Names:T3D7.2, T8I13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47170.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: UniProtKB-SubCell
  • Golgi apparatus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → N: Constitutively inactive form (GDP-locked form); loss of intracellular protein trafficking. 3 Publications1
Mutagenesisi71Q → L: Constitutively active form (GTP-locked form). 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074262 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP36397.
PRIDEiP36397.

PTM databases

iPTMnetiP36397.

Expressioni

Gene expression databases

GenevisibleiP36397. AT.

Interactioni

Subunit structurei

Interacts with AGD7 and GDAP1. GDP-locked form interacts with cytosolic tail of p24 proteins.3 Publications

Protein-protein interaction databases

BioGridi4665. 1 interactor.
IntActiP36397. 1 interactor.
STRINGi3702.AT2G47170.1.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 9Combined sources5
Helixi10 – 12Combined sources3
Beta strandi17 – 25Combined sources9
Helixi30 – 37Combined sources8
Beta strandi42 – 48Combined sources7
Beta strandi51 – 58Combined sources8
Beta strandi61 – 68Combined sources8
Helixi79 – 84Combined sources6
Beta strandi85 – 93Combined sources9
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 178Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AQ4X-ray1.80A/B1-181[»]
ProteinModelPortaliP36397.
SMRiP36397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000163691.
KOiK07937.
OMAiALRHRNW.
OrthoDBiEOG09360NRP.
PhylomeDBiP36397.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSFGKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV
110 120 130 140 150
VEARDELHRM LNEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ
160 170 180
RHWYIQSTCA TSGEGLYEGL DWLSNNIASK A
Length:181
Mass (Da):20,609
Last modified:January 23, 2007 - v2
Checksum:i7EDDEC87B7B29592
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95166 mRNA. Translation: AAA32729.1.
AC002337 Genomic DNA. Translation: AAB63817.1.
AC007236 Genomic DNA. Translation: AAM15469.1.
CP002685 Genomic DNA. Translation: AEC10810.1.
AY074859 mRNA. Translation: AAL75910.1.
AY142032 mRNA. Translation: AAM98296.1.
AY087342 mRNA. Translation: AAM64892.1.
PIRiS28875.
RefSeqiNP_182239.1. NM_130285.3.
UniGeneiAt.12215.
At.23262.

Genome annotation databases

EnsemblPlantsiAT2G47170.1; AT2G47170.1; AT2G47170.
GeneIDi819330.
GrameneiAT2G47170.1; AT2G47170.1; AT2G47170.
KEGGiath:AT2G47170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95166 mRNA. Translation: AAA32729.1.
AC002337 Genomic DNA. Translation: AAB63817.1.
AC007236 Genomic DNA. Translation: AAM15469.1.
CP002685 Genomic DNA. Translation: AEC10810.1.
AY074859 mRNA. Translation: AAL75910.1.
AY142032 mRNA. Translation: AAM98296.1.
AY087342 mRNA. Translation: AAM64892.1.
PIRiS28875.
RefSeqiNP_182239.1. NM_130285.3.
UniGeneiAt.12215.
At.23262.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AQ4X-ray1.80A/B1-181[»]
ProteinModelPortaliP36397.
SMRiP36397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4665. 1 interactor.
IntActiP36397. 1 interactor.
STRINGi3702.AT2G47170.1.

PTM databases

iPTMnetiP36397.

Proteomic databases

PaxDbiP36397.
PRIDEiP36397.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47170.1; AT2G47170.1; AT2G47170.
GeneIDi819330.
GrameneiAT2G47170.1; AT2G47170.1; AT2G47170.
KEGGiath:AT2G47170.

Organism-specific databases

TAIRiAT2G47170.

Phylogenomic databases

eggNOGiKOG0070. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000163691.
KOiK07937.
OMAiALRHRNW.
OrthoDBiEOG09360NRP.
PhylomeDBiP36397.

Enzyme and pathway databases

ReactomeiR-ATH-1660514. Synthesis of PIPs at the Golgi membrane.
R-ATH-421837. Clathrin derived vesicle budding.

Miscellaneous databases

PROiP36397.

Gene expression databases

GenevisibleiP36397. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF1_ARATH
AccessioniPrimary (citable) accession number: P36397
Secondary accession number(s): Q7G9L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.