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P36397

- ARF1_ARATH

UniProt

P36397 - ARF1_ARATH

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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes.5 Publications

Enzyme regulationi

Activated by AGD7 and AGD10.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: TAIR
  2. phospholipase activator activity Source: TAIR

GO - Biological processi

  1. positive regulation of catalytic activity Source: GOC
  2. protein transport Source: UniProtKB-KW
  3. small GTPase mediated signal transduction Source: InterPro
  4. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_187646. Synthesis of PIPs at the Golgi membrane.
REACT_235106. Clathrin derived vesicle budding.
REACT_254551. COPI Mediated Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Short name:
AtARF1
Gene namesi
Name:ARF1
Ordered Locus Names:At2g47170
ORF Names:T3D7.2, T8I13.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G47170.

Subcellular locationi

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. Golgi apparatus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311T → N: Constitutively inactive form (GDP-locked form); loss of intracellular protein trafficking. 3 Publications
Mutagenesisi71 – 711Q → L: Constitutively active form (GTP-locked form). 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 181180ADP-ribosylation factor 1PRO_0000207426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PRIDEiP36397.

Expressioni

Gene expression databases

GenevestigatoriP36397.

Interactioni

Subunit structurei

Interacts with AGD7 and GDAP1. GDP-locked form interacts with cytosolic tail of p24 proteins.3 Publications

Protein-protein interaction databases

BioGridi4665. 1 interaction.
IntActiP36397. 1 interaction.
STRINGi3702.AT2G47170.1-P.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 95Combined sources
Helixi10 – 123Combined sources
Beta strandi17 – 259Combined sources
Helixi30 – 378Combined sources
Beta strandi42 – 487Combined sources
Beta strandi51 – 588Combined sources
Beta strandi61 – 688Combined sources
Helixi79 – 846Combined sources
Beta strandi85 – 939Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11112Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1427Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AQ4X-ray1.80A/B1-181[»]
ProteinModelPortaliP36397.
SMRiP36397. Positions 4-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000163691.
KOiK07937.
OMAiHYFRNTE.
PhylomeDBiP36397.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36397-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLSFGKLFS RLFAKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRDRV
110 120 130 140 150
VEARDELHRM LNEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRQ
160 170 180
RHWYIQSTCA TSGEGLYEGL DWLSNNIASK A
Length:181
Mass (Da):20,609
Last modified:January 23, 2007 - v2
Checksum:i7EDDEC87B7B29592
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95166 mRNA. Translation: AAA32729.1.
AC002337 Genomic DNA. Translation: AAB63817.1.
AC007236 Genomic DNA. Translation: AAM15469.1.
CP002685 Genomic DNA. Translation: AEC10810.1.
AY074859 mRNA. Translation: AAL75910.1.
AY142032 mRNA. Translation: AAM98296.1.
AY087342 mRNA. Translation: AAM64892.1.
PIRiS28875.
RefSeqiNP_182239.1. NM_130285.2.
UniGeneiAt.12215.
At.23262.

Genome annotation databases

EnsemblPlantsiAT2G47170.1; AT2G47170.1; AT2G47170.
GeneIDi819330.
KEGGiath:AT2G47170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95166 mRNA. Translation: AAA32729.1 .
AC002337 Genomic DNA. Translation: AAB63817.1 .
AC007236 Genomic DNA. Translation: AAM15469.1 .
CP002685 Genomic DNA. Translation: AEC10810.1 .
AY074859 mRNA. Translation: AAL75910.1 .
AY142032 mRNA. Translation: AAM98296.1 .
AY087342 mRNA. Translation: AAM64892.1 .
PIRi S28875.
RefSeqi NP_182239.1. NM_130285.2.
UniGenei At.12215.
At.23262.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AQ4 X-ray 1.80 A/B 1-181 [» ]
ProteinModelPortali P36397.
SMRi P36397. Positions 4-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4665. 1 interaction.
IntActi P36397. 1 interaction.
STRINGi 3702.AT2G47170.1-P.

Proteomic databases

PRIDEi P36397.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G47170.1 ; AT2G47170.1 ; AT2G47170 .
GeneIDi 819330.
KEGGi ath:AT2G47170.

Organism-specific databases

TAIRi AT2G47170.

Phylogenomic databases

eggNOGi COG1100.
HOGENOMi HOG000163691.
KOi K07937.
OMAi HYFRNTE.
PhylomeDBi P36397.

Enzyme and pathway databases

Reactomei REACT_187646. Synthesis of PIPs at the Golgi membrane.
REACT_235106. Clathrin derived vesicle budding.
REACT_254551. COPI Mediated Transport.

Gene expression databases

Genevestigatori P36397.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family."
    Regad F., Bardet C., Tremousaygue D., Moisan A., Lescure B., Axelos M.
    FEBS Lett. 316:133-136(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "In situ localization and in vitro induction of plant COPI-coated vesicles."
    Pimpl P., Movafeghi A., Coughlan S., Denecke J., Hillmer S., Robinson D.G.
    Plant Cell 12:2219-2236(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis."
    Lee M.H., Min M.K., Lee Y.J., Jin J.B., Shin D.H., Kim D.H., Lee K.-H., Hwang I.
    Plant Physiol. 129:1507-1520(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-31, FUNCTION.
  8. "Arf1 GTPase plays roles in the protein traffic between the endoplasmic reticulum and the Golgi apparatus in tobacco and Arabidopsis cultured cells."
    Takeuchi M., Ueda T., Yahara N., Nakano A.
    Plant J. 31:499-515(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
  9. "The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole."
    Pimpl P., Hanton S.L., Taylor J.P., Pinto-daSilva L.L., Denecke J.
    Plant Cell 15:1242-1256(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-31 AND GLN-71, FUNCTION.
  10. "Sorting signals in the cytosolic tail of membrane proteins involved in the interaction with plant ARF1 and coatomer."
    Contreras I., Ortiz-Zapater E., Aniento F.
    Plant J. 38:685-698(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH P24 PROTEINS.
  11. "Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarity."
    Xu J., Scheres B.
    Plant Cell 17:525-536(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "RPA, a class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis."
    Song X.-F., Yang C.-Y., Liu J., Yang W.-C.
    Plant Physiol. 141:966-976(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  13. "Overexpression of Arabidopsis AGD7 causes relocation of Golgi-localized proteins to the endoplasmic reticulum and inhibits protein trafficking in plant cells."
    Min M.K., Kim S.J., Miao Y., Shin J., Jiang L., Hwang I.
    Plant Physiol. 143:1601-1614(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AGD7, ENZYME REGULATION.
  14. "Multiple roles of ADP-ribosylation factor 1 in plant cells include spatially regulated recruitment of coatomer and elements of the Golgi matrix."
    Matheson L.A., Hanton S.L., Rossi M., Latijnhouwers M., Stefano G., Renna L., Brandizzi F.
    Plant Physiol. 143:1615-1627(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GDAP1, FUNCTION.

Entry informationi

Entry nameiARF1_ARATH
AccessioniPrimary (citable) accession number: P36397
Secondary accession number(s): Q7G9L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3