Reviewed, UniProtKB/Swiss-Prot P36383 (CXG1_HUMAN)

Names and origin

Protein names

Recommended name:
    Gap junction gamma-1 protein
Alternative name(s):
    Gap junction alpha-7 protein
    Connexin-45
      Short name=Cx45

Gene names

Name:	GJC1
Synonyms:	GJA7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Sequence similarities	Belongs to the connexin family. Gamma-type subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane
Gene Ontology (GO)
Biological process	cell-cell junction assembly Ref.1 Traceable author statement. Source: ProtInc muscle contraction Ref.1 Traceable author statement. Source: ProtInc transport Ref.1 Non-traceable author statement. Source: ProtInc
Cellular component	connexon complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Gap junction gamma-1 protein		PRO_0000057826
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 45	23	Potential
	Topological domain	46 – 75	30	Extracellular Potential
	Transmembrane	76 – 95	20	Potential
	Topological domain	96 – 175	80	Cytoplasmic Potential
	Transmembrane	176 – 198	23	Potential
	Topological domain	199 – 228	30	Extracellular Potential
	Transmembrane	229 – 248	20	Potential
	Topological domain	249 – 396	148	Cytoplasmic Potential
Experimental info
	Sequence conflict	382	1	T → A in BAG54030. Ref.2
	Sequence conflict	392	1	T → N in AAA60458. Ref.1
	Sequence conflict	392	1	T → N in AAH96213. Ref.4
	Sequence conflict	392	1	T → N in AAH96214. Ref.4
	Sequence conflict	392	1	T → N in AAH96215. Ref.4
	Sequence conflict	392	1	T → N in AAH96216. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36383-1 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: C9F67C1D926D40DF	FASTA	396	45,470
	10 20 30 40 50 60 MSWSFLTRLL EEIHNHSTFV GKIWLTVLIV FRIVLTAVGG ESIYYDEQSK FVCNTEQPGC 70 80 90 100 110 120 ENVCYDAFAP LSHVRFWVFQ IILVATPSVM YLGYAIHKIA KMEHGEADKK AARSKPYAMR 130 140 150 160 170 180 WKQHRALEET EEDNEEDPMM YPEMELESDK ENKEQSQPKP KHDGRRRIRE DGLMKIYVLQ 190 200 210 220 230 240 LLARTVFEVG FLIGQYFLYG FQVHPFYVCS RLPCPHKIDC FISRPTEKTI FLLIMYGVTG 250 260 270 280 290 300 LCLLLNIWEM LHLGFGTIRD SLNSKRRELE DPGAYNYPFT WNTPSAPPGY NIAVKPDQIQ 310 320 330 340 350 360 YTELSNAKIA YKQNKANTAQ EQQYGSHEEN LPADLEALQR EIRMAQERLD LAVQAYSHQN 370 380 390 NPHGPREKKA KVGSKAGSNK STASSKSGDG KTSVWI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of two human cardiac gap junction proteins, connexin40 and connexin45." Kanter H.L., Saffitz J.E., Beyer E.C. J. Mol. Cell. Cardiol. 26:861-868(1994) [PubMed: 7966354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U03493 Genomic DNA. Translation: AAA60458.1. AK124339 mRNA. Translation: BAG54030.1. AC005180 Genomic DNA. No translation available. BC096213 mRNA. Translation: AAH96213.1. BC096214 mRNA. Translation: AAH96214.1. BC096215 mRNA. Translation: AAH96215.1. BC096216 mRNA. Translation: AAH96216.1.
IPI	IPI00002946.
PIR	I38430.
RefSeq	NP_001073852.1. NP_005488.2.
UniGene	Hs.532593 Hs.712052
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P36383.
Proteomic databases
PRIDE	P36383.
Genome annotation databases
Ensembl	ENSG00000182963. Homo sapiens. [Contig view]
GeneID	10052.
KEGG	hsa:10052.
Organism-specific databases
GeneCards	GC17M040237.
HGNC	HGNC:4280. GJC1.
MIM	608655. gene.
PharmGKB	PA28691.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P36383.
HOVERGEN	P36383.
OMA	P36383. SHEEHLP.
Enzyme and pathway databases
Reactome	REACT_9480. Gap junction trafficking and regulation. REACT_9492. Synthesis of Cx45.
Gene expression databases
ArrayExpress	P36383.
Bgee	P36383.
CleanEx	HS_GJC1.
GermOnline	ENSG00000182963. Homo sapiens.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002265. Connexin45. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. IPR017991. connexins_rgn. [Graphical view]
PANTHER	PTHR11984. Connexin. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01136. CONNEXINA6.
SMART	SM00037. CNX. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	37975.
SOURCE	Search...

Entry information

Entry name

CXG1_HUMAN

Accession

Primary (citable) accession number: P36383
Secondary accession number(s): B3KW68, Q4VAY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 14, 2009
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)