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Protein

Gap junction alpha-8 protein

Gene

GJA8

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction alpha-8 protein
Alternative name(s):
Connexin-45.6
Short name:
Cx45.6
Gene namesi
Name:GJA8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 20CytoplasmicSequence analysisAdd BLAST19
Transmembranei21 – 43HelicalSequence analysisAdd BLAST23
Topological domaini44 – 74ExtracellularSequence analysisAdd BLAST31
Transmembranei75 – 97HelicalSequence analysisAdd BLAST23
Topological domaini98 – 152CytoplasmicSequence analysisAdd BLAST55
Transmembranei153 – 175HelicalSequence analysisAdd BLAST23
Topological domaini176 – 207ExtracellularSequence analysisAdd BLAST32
Transmembranei208 – 230HelicalSequence analysisAdd BLAST23
Topological domaini231 – 400CytoplasmicSequence analysisAdd BLAST170

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi364S → A: Prevents degradation. 1 Publication1
Mutagenesisi365D → A: Prevents cleavage by caspase-3. 1 Publication1
Mutagenesisi368E → A: Prevents cleavage by caspase-3. 1 Publication1
Mutagenesisi368E → D: Increases cleavage by caspase-3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000578332 – 400Gap junction alpha-8 proteinAdd BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei364Phosphoserine; by CK22 Publications1

Post-translational modificationi

Proteolytically cleaved by caspase-3 during lens development.1 Publication
Phosphorylated on Ser-364; which inhibits cleavage by caspase-3.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei368 – 369Cleavage; by caspase-32

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP36381.

PTM databases

iPTMnetiP36381.

Miscellaneous databases

PMAP-CutDBiP36381.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. During early stages of lens development, interacts with the C-terminus of MIP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MIPP2823811EBI-867402,EBI-867385

Protein-protein interaction databases

IntActiP36381. 1 interactor.
STRINGi9031.ENSGALP00000036053.

Structurei

3D structure databases

SMRiP36381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF3Z. Eukaryota.
ENOG410ZC96. LUCA.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP36381.
KOiK07617.
PhylomeDBiP36381.

Family and domain databases

InterProiView protein in InterPro
IPR000500. Connexin.
IPR002266. Connexin50.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
PANTHERiPTHR11984. PTHR11984. 1 hit.
PTHR11984:SF19. PTHR11984:SF19. 1 hit.
PfamiView protein in Pfam
PF00029. Connexin. 1 hit.
PF03509. Connexin50. 1 hit.
PRINTSiPR00206. CONNEXIN.
PR01137. CONNEXINA8.
SMARTiView protein in SMART
SM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
PROSITEiView protein in PROSITE
PS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWSFLGNI LEQVNEQSTV IGRVWLTVLF IFRILILGTA AELVWGDEQS
60 70 80 90 100
DFVCNTQQPG CENVCYDEAF PISHIRLWVL QIIFVSTPSL VYFGHAVHHV
110 120 130 140 150
RMEEKRKERE EAERRQQAEV DEEKLPLAPN QNKGNNPDGT KKFRLEGTLL
160 170 180 190 200
RTYILHIIFK TLFEVGFIVG QYFLYGFRIL PLYRCGRWPC PNLVDCFVSR
210 220 230 240 250
PTEKTIFIMF MLVVAAVSLF LNLVEISHLI LKRIRRALRR PAEEQMGEVP
260 270 280 290 300
EKPLHAIAVS SIPKAKGYKL LEEEKPVSHY FPLTEVGVEP SPLPSAFNEF
310 320 330 340 350
EEKIGMGPLE DLSRAFDERL PSYAQAKEPE EEKVKAEEEE EQEEEQQAPQ
360 370 380 390 400
EEPGVKKAEE EVVSDEVEGP SAPAELATDV RSLSRLSKAS SRARSDDLTV
Length:400
Mass (Da):45,616
Last modified:January 23, 2007 - v2
Checksum:i6B380599F37C0856
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146 – 147EG → DL in BAA05381 (PubMed:8840185).Curated2
Sequence conflicti188 – 189WP → D in BAA05381 (PubMed:8840185).Curated2
Sequence conflicti234 – 236IRR → SEL in BAA05381 (PubMed:8840185).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24799 Genomic DNA. Translation: AAA57134.1.
D26333 mRNA. Translation: BAA05381.1.
PIRiI50219.
RefSeqiNP_990328.1. NM_204997.1.
UniGeneiGga.498.
Gga.51510.

Genome annotation databases

GeneIDi395846.
KEGGigga:395846.

Similar proteinsi

Entry informationi

Entry nameiCXA8_CHICK
AccessioniPrimary (citable) accession number: P36381
Secondary accession number(s): Q92144
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families