Reviewed, UniProtKB/Swiss-Prot P36376 (KLK12_RAT)

Names and origin

Protein names

Recommended name:
    Glandular kallikrein-12, submandibular/renal
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein
    RSKG-3

Gene names

Name:	Klk12
Synonyms:	Klk-12

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Tissue specificity	Kidney and submandibular gland.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	positive regulation of acute inflammatory response Inferred from mutant phenotype. Source: RGD positive regulation of apoptosis Inferred from mutant phenotype. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype. Source: RGD proteolysis Inferred from electronic annotation. Source: InterPro response to nutrient Inferred from expression pattern. Source: RGD
Cellular component	acrosomal vesicle Inferred from direct assay. Source: RGD apical part of cell Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD
Molecular function	serine-type endopeptidase activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000028017
	Chain	25 – 259	235	Glandular kallikrein-12, submandibular/renal		PRO_0000028018
Regions
	Domain	25 – 256	232	Peptidase S1
Sites
	Active site	63	1	Charge relay system
	Active site	118	1	Charge relay system
	Active site	211	1	Charge relay system
Amino acid modifications
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 171		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	150 ↔ 217		By similarity
	Disulfide bond	182 ↔ 196		By similarity
	Disulfide bond	207 ↔ 232		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36376-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 535A8EE25435144F	FASTA	259	28,759
	10 20 30 40 50 60 MWLQILFLVL SVGRIDAAPP GQSRVVGGYK CEKNSQPWQV AVINRYLCGG VLIDPSWVIT 70 80 90 100 110 120 AAHCYSHNYH VLLGRNNLFK DEPFAQYRVV NQSFPHPDYN PFFMKNHTLF PGDDHSNDLM 130 140 150 160 170 180 LLHLSEPADI TDGVKVIDLP TEEPKVGSTC LASGWSSTKP LEWEFPDDLQ CVNINILSNE 190 200 210 220 230 240 KCIKAHTQMV TDVMLCAGEL EGGKDTCNGD SGGPLLCDGV LQGITSWSSV PCGETNRPAI 250 YTKLIKFTSW IKEVMKENS

References

[1]	"Molecular cloning and characterization of two rat renal kallikrein genes." Chen Y.-P., Chao J., Chao L. Biochemistry 27:7189-7196(1988) [PubMed: 2849988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19648, M22922 mRNA. Translation: AAA51640.1.
IPI	IPI00201595.
PIR	B31136.
RefSeq	NP_001005382.1.
UniGene	Rn.141980
3D structure databases
HSSP	HSSP built from PDB template 1TON based on UniProtKB P00759.
SMR	P36376. Positions 25-258.
ModBase	Search...
Protein family/group databases
MEROPS	S01.287.
Genome annotation databases
Ensembl	ENSRNOG00000033050. Rattus norvegicus. [Contig view]
GeneID	292855.
KEGG	rno:292855.
Organism-specific databases
RGD	1303192. Klk12.
Phylogenomic databases
HOVERGEN	P36376.
Enzyme and pathway databases
BRENDA	3.4.21.35. 248.
Gene expression databases
ArrayExpress	P36376.
GermOnline	ENSRNOG00000033050. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	634931.

Entry information

Entry name

KLK12_RAT

Accession

Primary (citable) accession number: P36376

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)