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P36376 (KLK12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glandular kallikrein-12, submandibular/renal

EC=3.4.21.35
Alternative name(s):
RSKG-3
Tissue kallikrein
Gene names
Name:Klk12
Synonyms:Klk-12
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Tissue specificity

Kidney and submandibular gland.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide Probable
PRO_0000028017
Chain25 – 259235Glandular kallikrein-12, submandibular/renal
PRO_0000028018

Regions

Domain25 – 256232Peptidase S1

Sites

Active site631Charge relay system
Active site1181Charge relay system
Active site2111Charge relay system

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 171 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond150 ↔ 217 By similarity
Disulfide bond182 ↔ 196 By similarity
Disulfide bond207 ↔ 232 By similarity

Sequences

Sequence LengthMass (Da)Tools
P36376 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 535A8EE25435144F

FASTA25928,759
        10         20         30         40         50         60 
MWLQILFLVL SVGRIDAAPP GQSRVVGGYK CEKNSQPWQV AVINRYLCGG VLIDPSWVIT 

        70         80         90        100        110        120 
AAHCYSHNYH VLLGRNNLFK DEPFAQYRVV NQSFPHPDYN PFFMKNHTLF PGDDHSNDLM 

       130        140        150        160        170        180 
LLHLSEPADI TDGVKVIDLP TEEPKVGSTC LASGWSSTKP LEWEFPDDLQ CVNINILSNE 

       190        200        210        220        230        240 
KCIKAHTQMV TDVMLCAGEL EGGKDTCNGD SGGPLLCDGV LQGITSWSSV PCGETNRPAI 

       250 
YTKLIKFTSW IKEVMKENS 

« Hide

References

[1]"Molecular cloning and characterization of two rat renal kallikrein genes."
Chen Y.-P., Chao J., Chao L.
Biochemistry 27:7189-7196(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19648, M22922 mRNA. Translation: AAA51640.1.
PIRB31136.
RefSeqNP_001005382.1. NM_001005382.1.
UniGeneRn.141980.

3D structure databases

ProteinModelPortalP36376.
SMRP36376. Positions 25-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025631.

Protein family/group databases

MEROPSS01.287.

Proteomic databases

PRIDEP36376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID292855.
KEGGrno:292855.
UCSCRGD:1303192. rat.

Organism-specific databases

CTD292855.
RGD1303192. Klk12.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP36376.
KOK01325.
PhylomeDBP36376.

Gene expression databases

GenevestigatorP36376.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio634931.

Entry information

Entry nameKLK12_RAT
AccessionPrimary (citable) accession number: P36376
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries