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Protein

Glandular kallikrein-10

Gene

Klk10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This protein may be involved in the regulation of renal function.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay system1
Active sitei118Charge relay system1
Active sitei211Charge relay system1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-RNO-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)

Protein family/group databases

MEROPSiS01.165

Names & Taxonomyi

Protein namesi
Recommended name:
Glandular kallikrein-10 (EC:3.4.21.35)
Short name:
K10
Short name:
rGK-10
Alternative name(s):
Endopeptidase K
Proteinase B
T-kininogenase
Tissue kallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Klk10
Synonyms:Klk-10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1303242 Klk10

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002801319 – 24Activation peptide3 Publications6
ChainiPRO_000002801425 – 259Glandular kallikrein-10Add BLAST235
ChainiPRO_000002801525 – 111T-kininogenase light chainAdd BLAST87
ChainiPRO_0000028016112 – 259T-kininogenase heavy chainAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 171PROSITE-ProRule annotation
Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi150 ↔ 217PROSITE-ProRule annotation
Disulfide bondi182 ↔ 196PROSITE-ProRule annotation
Disulfide bondi207 ↔ 232PROSITE-ProRule annotation

Post-translational modificationi

Probably N- and O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP36375
PRIDEiP36375

PTM databases

iPTMnetiP36375
PhosphoSitePlusiP36375

Expressioni

Tissue specificityi

Kidney and submandibular gland, where it is found in the granular convoluted tubule and striated duct cells. It is likely that the enzyme is mainly synthesized in the granular convoluted tubules and then transferred to other tissues by release into the vasculature or interstitial space.1 Publication

Gene expression databases

BgeeiENSRNOG00000046297
GenevisibleiP36375 RN

Interactioni

Subunit structurei

Heterodimer of a light chain and heavy chain linked by a disulfide bond.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050040

Structurei

3D structure databases

ProteinModelPortaliP36375
SMRiP36375
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 256Peptidase S1PROSITE-ProRule annotationAdd BLAST232

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144067
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP36375
KOiK01325
OMAiCANSNCE
OrthoDBiEOG091G0DF7
PhylomeDBiP36375
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP GQSRIVGGYK CEKNSQPWQV AIINEYLCGG
60 70 80 90 100
VLIDPSWVIT AAHCYSNYYH VLLGRNNLFE DEPFAQYRFV NQSFPHPDYK
110 120 130 140 150
PFLMRNHTRQ RGDDYSNDLM LLHLSEPADI TDGVKVIDLP TEEPKVGSTC
160 170 180 190 200
LASGWGSTKP LNWELPDDLQ CVNIHLLSNE KCIEAYEQKV TDLMLCAGEM
210 220 230 240 250
DGRKDTCKGD SGGPLICDGV LQGITSWGNV PCAEPYNPGV YTKLIKFTSW

IKEVMKENP
Length:259
Mass (Da):28,981
Last modified:June 12, 2007 - v2
Checksum:i18DBC31C6086E5F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44N → IET AA sequence (PubMed:2303430).Curated1
Sequence conflicti130 – 131IT → DS AA sequence (PubMed:3482210).Curated2
Sequence conflicti143E → G AA sequence (PubMed:2303430).Curated1
Sequence conflicti148S → G AA sequence (PubMed:2303430).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000574 Genomic DNA No translation available.
S48142 mRNA Translation: AAB24071.1
PIRiA44284
B35545
RefSeqiNP_001128645.1, NM_001135173.1
XP_003748896.1, XM_003748848.3
UniGeneiRn.220730

Genome annotation databases

EnsembliENSRNOT00000041899; ENSRNOP00000048581; ENSRNOG00000046670
ENSRNOT00000045756; ENSRNOP00000050040; ENSRNOG00000046297
GeneIDi100911689
292858
KEGGirno:100911689
rno:292858
UCSCiRGD:1303242 rat

Similar proteinsi

Entry informationi

Entry nameiKLK10_RAT
AccessioniPrimary (citable) accession number: P36375
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 12, 2007
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health