Reviewed, UniProtKB/Swiss-Prot P36374 (KLK6_RAT)

Names and origin

Protein names

Recommended name:
    Prostatic glandular kallikrein-6
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein
    P1 kallikrein
    RGK-8

Gene names

Name:	Klk6
Synonyms:	Klk-8, Klk8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000028007
	Chain	25 – 261	237	Prostatic glandular kallikrein-6		PRO_0000028008
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36374-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: CA8F12151B04E337	FASTA	261	29,013
	10 20 30 40 50 60 MWLLILFLIL SLGWNDAAPP GQSRIIGGFN CEKNSQPWQV AVYHFNEPQC GGVLIHPSWV 70 80 90 100 110 120 ITAAHCYSVN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPG FNLDIIKNHT RKPGNDYSND 130 140 150 160 170 180 LMLLHLKTPA DITDGVKVID LPTEEPKVGS TCLTSGWGSI TPLKWEFPDD LQCVNIHLLS 190 200 210 220 230 240 NEKCIKAYND EVTDVMLCAG EMDGGKDICK GDSGGPLICD GVLQGITSWG SMPCGEPNKP 250 260 SVYTKLIKFT SWMKKVMKEN P

References

[1]	"Expression of two kallikrein gene family members in the rat prostate." Brady J.M., Wines D.R., MacDonald R.J. Biochemistry 28:5203-5210(1989) [PubMed: 2765531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27217, M27215, M27216 Genomic DNA. Translation: AAA42036.1.
IPI	IPI00201593.
PIR	A34079.
RefSeq	NP_001013085.1.
UniGene	Rn.79264
3D structure databases
HSSP	HSSP built from PDB template 1TON based on UniProtKB P00759.
SMR	P36374. Positions 25-261.
ModBase	Search...
Protein family/group databases
MEROPS	S01.288.
Genome annotation databases
Ensembl	ENSRNOG00000029596. Rattus norvegicus. [Contig view]
GeneID	292866.
KEGG	rno:292866.
Organism-specific databases
RGD	1305359. Klk6.
Phylogenomic databases
HOVERGEN	P36374.
Enzyme and pathway databases
BRENDA	3.4.21.118. 248. 3.4.21.35. 248.
Gene expression databases
ArrayExpress	P36374.
GermOnline	ENSRNOG00000029596. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	634943.

Entry information

Entry name

KLK6_RAT

Accession

Primary (citable) accession number: P36374

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	March 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)