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P36373

- KLK7_RAT

UniProt

P36373 - KLK7_RAT

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Protein

Glandular kallikrein-7, submandibular/renal

Gene

Klk7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Predominant kallikrein protein in the kidney.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.406.

Names & Taxonomyi

Protein namesi
Recommended name:
Glandular kallikrein-7, submandibular/renal (EC:3.4.21.35)
Short name:
rGK-7
Alternative name(s):
Esterase B
Kallikrein-related protein K1
Proteinase A
RSKG-7
Tissue kallikrein
Gene namesi
Name:Klk7
Synonyms:Klk-7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1306420. Klk7.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Propeptidei19 – 246Activation peptide2 PublicationsPRO_0000028005
Chaini25 – 261237Glandular kallikrein-7, submandibular/renalPRO_0000028006Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP36373.

PTM databases

PhosphoSiteiP36373.

Expressioni

Tissue specificityi

Kidney and submandibular gland. Not expressed in liver, pancreas, spleen, parotid, testis, cortex, prostate, ovary and pituitary.1 Publication

Gene expression databases

GenevestigatoriP36373.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025750.

Structurei

3D structure databases

ProteinModelPortaliP36373.
SMRiP36373. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP36373.
KOiK01325.
PhylomeDBiP36373.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36373 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWFLILFLDL SLGQIDAAPP GQSRVIGGYK CEKNSQPWQV ALYSFTKYLC
60 70 80 90 100
GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD
110 120 130 140 150
YKPFLMRNHT RKPGDDHSND LMLLHLSQPA DITDGVKVID LPTEEPKVGS
160 170 180 190 200
TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG
210 220 230 240 250
ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT
260
SWIKEVMKEN P
Length:261
Mass (Da):28,972
Last modified:June 1, 1994 - v1
Checksum:i4FB06C422F25AF16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351S → D AA sequence (PubMed:3482210)Curated
Sequence conflicti46 – 461T → S AA sequence (PubMed:3482210)Curated
Sequence conflicti46 – 461T → S AA sequence (PubMed:2183721)Curated
Sequence conflicti115 – 1151D → A AA sequence (PubMed:2183721)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19647 Genomic DNA. Translation: AAA41461.1.
PIRiA31136.
RefSeqiNP_036725.1. NM_012593.1.
UniGeneiRn.207160.

Genome annotation databases

GeneIDi24523.
KEGGirno:24523.
UCSCiRGD:1306420. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19647 Genomic DNA. Translation: AAA41461.1 .
PIRi A31136.
RefSeqi NP_036725.1. NM_012593.1.
UniGenei Rn.207160.

3D structure databases

ProteinModelPortali P36373.
SMRi P36373. Positions 25-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025750.

Protein family/group databases

MEROPSi S01.406.

PTM databases

PhosphoSitei P36373.

Proteomic databases

PRIDEi P36373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24523.
KEGGi rno:24523.
UCSCi RGD:1306420. rat.

Organism-specific databases

CTDi 24523.
RGDi 1306420. Klk7.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P36373.
KOi K01325.
PhylomeDBi P36373.

Miscellaneous databases

NextBioi 603573.

Gene expression databases

Genevestigatori P36373.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of two rat renal kallikrein genes."
    Chen Y.-P., Chao J., Chao L.
    Biochemistry 27:7189-7196(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of serine proteinases isolated from rat submaxillary gland: with special reference to the degradation of rat kininogens by these enzymes."
    Kato H., Nakanishi E., Enjyoji K., Hayashi I., Oh-Ishi S., Iwanaga S.
    J. Biochem. 102:1389-1404(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-75.
    Tissue: Submandibular gland.
  3. "Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland."
    Elmoujahed A., Gutman N., Brillard M., Gauthier F.
    FEBS Lett. 265:137-140(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-36.
    Tissue: Submandibular gland.
  4. "The expression of two kallikrein gene family members in the rat kidney."
    Brady J.M., MacDonald R.J.
    Arch. Biochem. Biophys. 278:342-349(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 43-261, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKLK7_RAT
AccessioniPrimary (citable) accession number: P36373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3