Reviewed, UniProtKB/Swiss-Prot P36373 (KLK7_RAT)

Names and origin

Protein names

Recommended name:
    Glandular kallikrein-7, submandibular/renal
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein
    RGK-7
    RSKG-7
    Kallikrein-related protein K1
    Esterase B
    Proteinase A

Gene names

Name:	Klk7
Synonyms:	Klk-7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Predominant kallikrein protein in the kidney.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Tissue specificity	Kidney and submandibular gland. Not expressed in liver, pancreas, spleen, parotid, testis, cortex, prostate, ovary and pituitary. Ref.4
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000028005
	Chain	25 – 261	237	Glandular kallikrein-7, submandibular/renal		PRO_0000028006
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity
Experimental info
	Sequence conflict	35	1	S → D AA sequence Ref.2
	Sequence conflict	46	1	T → S AA sequence Ref.2
	Sequence conflict	46	1	T → S AA sequence Ref.4
	Sequence conflict	115	1	D → A AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36373-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 4FB06C422F25AF16	FASTA	261	28,972
	10 20 30 40 50 60 MWFLILFLDL SLGQIDAAPP GQSRVIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV 70 80 90 100 110 120 ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSND 130 140 150 160 170 180 LMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS 190 200 210 220 230 240 NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP 250 260 AIYTKLIKFT SWIKEVMKEN P

References

[1]	"Molecular cloning and characterization of two rat renal kallikrein genes." Chen Y.-P., Chao J., Chao L. Biochemistry 27:7189-7196(1988) [PubMed: 2849988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of serine proteinases isolated from rat submaxillary gland: with special reference to the degradation of rat kininogens by these enzymes." Kato H., Nakanishi E., Enjyoji K., Hayashi I., Oh-Ishi S., Iwanaga S. J. Biochem. 102:1389-1404(1987) [PubMed: 3482210] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-75. Tissue: Submandibular gland.
[3]	"Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland." Elmoujahed A., Gutman N., Brillard M., Gauthier F. FEBS Lett. 265:137-140(1990) [PubMed: 2194829] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-36. Tissue: Submandibular gland.
[4]	"The expression of two kallikrein gene family members in the rat kidney." Brady J.M., MacDonald R.J. Arch. Biochem. Biophys. 278:342-349(1990) [PubMed: 2183721] [Abstract] Cited for: PROTEIN SEQUENCE OF 43-261, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19647 Genomic DNA. Translation: AAA41461.1.
IPI	IPI00201592.
PIR	A31136.
RefSeq	NP_036725.1.
UniGene	Rn.207160
3D structure databases
HSSP	HSSP built from PDB template 1TON based on UniProtKB P00759.
SMR	P36373. Positions 25-261.
ModBase	Search...
Protein family/group databases
MEROPS	S01.406.
Genome annotation databases
Ensembl	ENSRNOG00000029924. Rattus norvegicus. [Contig view]
GeneID	24523.
KEGG	rno:24523.
Organism-specific databases
RGD	1306420. Klk7.
Phylogenomic databases
HOVERGEN	P36373.
Enzyme and pathway databases
BRENDA	3.4.21.35. 248.
Gene expression databases
ArrayExpress	P36373.
GermOnline	ENSRNOG00000029924. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603573.

Entry information

Entry name

KLK7_RAT

Accession

Primary (citable) accession number: P36373

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 5, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)