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P36373 (KLK7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glandular kallikrein-7, submandibular/renal

Short name=rGK-7
EC=3.4.21.35
Alternative name(s):
Esterase B
Kallikrein-related protein K1
Proteinase A
RSKG-7
Tissue kallikrein
Gene names
Name:Klk7
Synonyms:Klk-7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Predominant kallikrein protein in the kidney.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Tissue specificity

Kidney and submandibular gland. Not expressed in liver, pancreas, spleen, parotid, testis, cortex, prostate, ovary and pituitary. Ref.4

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Probable
Propeptide19 – 246Activation peptide Probable
PRO_0000028005
Chain25 – 261237Glandular kallikrein-7, submandibular/renal
PRO_0000028006

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system
Active site1201Charge relay system
Active site2131Charge relay system

Amino acid modifications

Glycosylation1081N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 173 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond152 ↔ 219 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond209 ↔ 234 By similarity

Experimental info

Sequence conflict351S → D AA sequence Ref.2
Sequence conflict461T → S AA sequence Ref.2
Sequence conflict461T → S AA sequence Ref.4
Sequence conflict1151D → A AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P36373 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 4FB06C422F25AF16

FASTA26128,972
        10         20         30         40         50         60 
MWFLILFLDL SLGQIDAAPP GQSRVIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV 

        70         80         90        100        110        120 
ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSND 

       130        140        150        160        170        180 
LMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS 

       190        200        210        220        230        240 
NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP 

       250        260 
AIYTKLIKFT SWIKEVMKEN P 

« Hide

References

[1]"Molecular cloning and characterization of two rat renal kallikrein genes."
Chen Y.-P., Chao J., Chao L.
Biochemistry 27:7189-7196(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Characterization of serine proteinases isolated from rat submaxillary gland: with special reference to the degradation of rat kininogens by these enzymes."
Kato H., Nakanishi E., Enjyoji K., Hayashi I., Oh-Ishi S., Iwanaga S.
J. Biochem. 102:1389-1404(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-75.
Tissue: Submandibular gland.
[3]"Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland."
Elmoujahed A., Gutman N., Brillard M., Gauthier F.
FEBS Lett. 265:137-140(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-36.
Tissue: Submandibular gland.
[4]"The expression of two kallikrein gene family members in the rat kidney."
Brady J.M., MacDonald R.J.
Arch. Biochem. Biophys. 278:342-349(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 43-261, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19647 Genomic DNA. Translation: AAA41461.1.
PIRA31136.
RefSeqNP_036725.1. NM_012593.1.
UniGeneRn.207160.

3D structure databases

ProteinModelPortalP36373.
SMRP36373. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025750.

Protein family/group databases

MEROPSS01.406.

PTM databases

PhosphoSiteP36373.

Proteomic databases

PRIDEP36373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24523.
KEGGrno:24523.
UCSCRGD:1306420. rat.

Organism-specific databases

CTD24523.
RGD1306420. Klk7.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
KOK01325.
PhylomeDBP36373.

Gene expression databases

GenevestigatorP36373.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603573.

Entry information

Entry nameKLK7_RAT
AccessionPrimary (citable) accession number: P36373
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries