Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P36373

- KLK7_RAT

UniProt

P36373 - KLK7_RAT

Protein

Glandular kallikrein-7, submandibular/renal

Gene

Klk7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Predominant kallikrein protein in the kidney.

    Catalytic activityi

    Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay system
    Active sitei120 – 1201Charge relay system
    Active sitei213 – 2131Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.406.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glandular kallikrein-7, submandibular/renal (EC:3.4.21.35)
    Short name:
    rGK-7
    Alternative name(s):
    Esterase B
    Kallikrein-related protein K1
    Proteinase A
    RSKG-7
    Tissue kallikrein
    Gene namesi
    Name:Klk7
    Synonyms:Klk-7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1306420. Klk7.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818CuratedAdd
    BLAST
    Propeptidei19 – 246Activation peptide2 PublicationsPRO_0000028005
    Chaini25 – 261237Glandular kallikrein-7, submandibular/renalPRO_0000028006Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 173PROSITE-ProRule annotation
    Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi152 ↔ 219PROSITE-ProRule annotation
    Disulfide bondi184 ↔ 198PROSITE-ProRule annotation
    Disulfide bondi209 ↔ 234PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiP36373.

    PTM databases

    PhosphoSiteiP36373.

    Expressioni

    Tissue specificityi

    Kidney and submandibular gland. Not expressed in liver, pancreas, spleen, parotid, testis, cortex, prostate, ovary and pituitary.1 Publication

    Gene expression databases

    GenevestigatoriP36373.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025750.

    Structurei

    3D structure databases

    ProteinModelPortaliP36373.
    SMRiP36373. Positions 25-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    KOiK01325.
    PhylomeDBiP36373.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWFLILFLDL SLGQIDAAPP GQSRVIGGYK CEKNSQPWQV ALYSFTKYLC    50
    GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD 100
    YKPFLMRNHT RKPGDDHSND LMLLHLSQPA DITDGVKVID LPTEEPKVGS 150
    TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG 200
    ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT 250
    SWIKEVMKEN P 261
    Length:261
    Mass (Da):28,972
    Last modified:June 1, 1994 - v1
    Checksum:i4FB06C422F25AF16
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351S → D AA sequence (PubMed:3482210)Curated
    Sequence conflicti46 – 461T → S AA sequence (PubMed:3482210)Curated
    Sequence conflicti46 – 461T → S AA sequence (PubMed:2183721)Curated
    Sequence conflicti115 – 1151D → A AA sequence (PubMed:2183721)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19647 Genomic DNA. Translation: AAA41461.1.
    PIRiA31136.
    RefSeqiNP_036725.1. NM_012593.1.
    UniGeneiRn.207160.

    Genome annotation databases

    GeneIDi24523.
    KEGGirno:24523.
    UCSCiRGD:1306420. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19647 Genomic DNA. Translation: AAA41461.1 .
    PIRi A31136.
    RefSeqi NP_036725.1. NM_012593.1.
    UniGenei Rn.207160.

    3D structure databases

    ProteinModelPortali P36373.
    SMRi P36373. Positions 25-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025750.

    Protein family/group databases

    MEROPSi S01.406.

    PTM databases

    PhosphoSitei P36373.

    Proteomic databases

    PRIDEi P36373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24523.
    KEGGi rno:24523.
    UCSCi RGD:1306420. rat.

    Organism-specific databases

    CTDi 24523.
    RGDi 1306420. Klk7.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    KOi K01325.
    PhylomeDBi P36373.

    Miscellaneous databases

    NextBioi 603573.

    Gene expression databases

    Genevestigatori P36373.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of two rat renal kallikrein genes."
      Chen Y.-P., Chao J., Chao L.
      Biochemistry 27:7189-7196(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of serine proteinases isolated from rat submaxillary gland: with special reference to the degradation of rat kininogens by these enzymes."
      Kato H., Nakanishi E., Enjyoji K., Hayashi I., Oh-Ishi S., Iwanaga S.
      J. Biochem. 102:1389-1404(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-75.
      Tissue: Submandibular gland.
    3. "Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland."
      Elmoujahed A., Gutman N., Brillard M., Gauthier F.
      FEBS Lett. 265:137-140(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-36.
      Tissue: Submandibular gland.
    4. "The expression of two kallikrein gene family members in the rat kidney."
      Brady J.M., MacDonald R.J.
      Arch. Biochem. Biophys. 278:342-349(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 43-261, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiKLK7_RAT
    AccessioniPrimary (citable) accession number: P36373
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3