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Protein

Antigen peptide transporter 2

Gene

Tap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi503 – 510ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ADP binding Source: RGD
  • ATP binding Source: UniProtKB
  • MHC class I protein binding Source: UniProtKB
  • nucleotide binding Source: RGD
  • peptide antigen-transporting ATPase activity Source: RGD
  • peptide transporter activity Source: InterPro
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 2
Short name:
APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Gene namesi
Name:Tap2
Synonyms:Abcb3, Mtp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3818. Tap2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalSequence analysis6
Transmembranei7 – 27Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini28 – 56CytoplasmicSequence analysisAdd BLAST29
Transmembranei57 – 77Helical; Name=2PROSITE-ProRule annotationAdd BLAST21
Topological domaini78 – 98LumenalSequence analysisAdd BLAST21
Transmembranei99 – 119Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini120 – 148CytoplasmicSequence analysisAdd BLAST29
Transmembranei149 – 169Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini170 – 187LumenalSequence analysisAdd BLAST18
Transmembranei188 – 208Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini209 – 266CytoplasmicSequence analysisAdd BLAST58
Transmembranei267 – 287Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini288 – 293LumenalSequence analysis6
Transmembranei294 – 314Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini315 – 374CytoplasmicSequence analysisAdd BLAST60
Transmembranei375 – 395Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini396 – 408LumenalSequence analysisAdd BLAST13
Transmembranei409 – 429Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini430 – 703CytoplasmicSequence analysisAdd BLAST274

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: RGD
  • MHC class I peptide loading complex Source: RGD
  • TAP complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933311 – 703Antigen peptide transporter 2Add BLAST703

Proteomic databases

PaxDbiP36372.
PRIDEiP36372.

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2.

Binary interactionsi

WithEntry#Exp.IntActNotes
gNQ77CE42EBI-11304494,EBI-11303846From a different organism.

GO - Molecular functioni

  • MHC class I protein binding Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi246934. 7 interactors.
IntActiP36372. 3 interactors.
STRINGi10116.ENSRNOP00000067594.

Structurei

3D structure databases

ProteinModelPortaliP36372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 435ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini468 – 702ABC transporterPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni301 – 389Involved in peptide-binding siteBy similarityAdd BLAST89
Regioni414 – 433Involved in peptide-binding siteBy similarityAdd BLAST20

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiP36372.
KOiK05654.
PhylomeDBiP36372.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSHPRPWA SLLLVDLALL GLLQSSLGTL LPPGLPGLWL EGTLRLGVLW
60 70 80 90 100
GLLKVGGLLR LVGTFLPLLC LTNPLFFSLR ALVGSTMSTS VVRVASASWG
110 120 130 140 150
WLLADYGAVA LSLAVWAVLS PAGAQEKEPG QENNRALMIR LLRLSKPDLP
160 170 180 190 200
FLIVAFIFLA MAVWWEMFIP HYSGRVIDIL GGDFDPDAFA SAIFFMCLFS
210 220 230 240 250
VGSSLSAGCR GGSFLFAESR INLRIREQLF SSLLRQDLAF FQETKTGELN
260 270 280 290 300
SRLSSDTSLM SQWLSLNANI LLRSLVKVVG LYYFMLQVSP RLTFLSLLDL
310 320 330 340 350
PLTIAAEKVY NPRHQAVLKE IQDAVAKAGQ VVREAVGGLQ TVRSFGAEEQ
360 370 380 390 400
EVRRYKEALE RCRQLWWRRD LEKSLYLVIQ RVMALGMQVL ILNVGVQQIL
410 420 430 440 450
AGEVTRGGLL SFLLYQEEVG HHVQNLVYMY GDMLSNVGAA EKVFSYLDRR
460 470 480 490 500
PNLPNPGTLA PPRLEGRVEF QDVSFSYPSR PEKPVLQGLT FTLHPGKVTA
510 520 530 540 550
LVGPNGSGKS TVAALLQNLY QPTGGQLLLD GEPLVQYDHH YLHRQVVLVG
560 570 580 590 600
QEPVLFSGSV KDNIAYGLRD CEDAQVMAAA QAACADDFIG EMTNGINTEI
610 620 630 640 650
GEKGSQLAVG QKQRLAIARA LVRNPRVLIL DEATSALDAE CEQALQTWRS
660 670 680 690 700
QEDRTMLVIA HRLHTVQNAD QVLVLKQGQL VEHDQLRDEQ DVYAHLVQQR

LEA
Length:703
Mass (Da):77,713
Last modified:June 1, 1994 - v1
Checksum:iE545993A8F784250
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti352V → F in TAP2L. 1 Publication1
Natural varianti603K → R in TAP2L. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63854 mRNA. Translation: CAA45339.1.
X75305 mRNA. Translation: CAA53053.1.
PIRiS38400.
RefSeqiNP_114445.2. NM_032056.3.
UniGeneiRn.202962.

Genome annotation databases

GeneIDi24812.
KEGGirno:24812.
UCSCiRGD:3818. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63854 mRNA. Translation: CAA45339.1.
X75305 mRNA. Translation: CAA53053.1.
PIRiS38400.
RefSeqiNP_114445.2. NM_032056.3.
UniGeneiRn.202962.

3D structure databases

ProteinModelPortaliP36372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246934. 7 interactors.
IntActiP36372. 3 interactors.
STRINGi10116.ENSRNOP00000067594.

Proteomic databases

PaxDbiP36372.
PRIDEiP36372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24812.
KEGGirno:24812.
UCSCiRGD:3818. rat.

Organism-specific databases

CTDi6891.
RGDi3818. Tap2.

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiP36372.
KOiK05654.
PhylomeDBiP36372.

Miscellaneous databases

PROiP36372.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAP2_RAT
AccessioniPrimary (citable) accession number: P36372
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 5, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.