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Protein

Antigen peptide transporter 2

Gene

Tap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi503 – 5108ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ADP binding Source: RGD
  • ATP binding Source: UniProtKB
  • MHC class I protein binding Source: UniProtKB
  • nucleotide binding Source: RGD
  • peptide antigen-transporting ATPase activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation of endogenous peptide antigen via MHC class I Source: MGI
  • peptide antigen transport Source: RGD
  • peptide transport Source: RGD
  • positive regulation of antigen processing and presentation of peptide antigen via MHC class I Source: RGD
  • positive regulation of T cell mediated cytotoxicity Source: MGI
  • protection from natural killer cell mediated cytotoxicity Source: RGD
  • protein complex assembly Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 2
Short name:
APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Gene namesi
Name:Tap2
Synonyms:Abcb3, Mtp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3818. Tap2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66LumenalSequence analysis
Transmembranei7 – 2721Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini28 – 5629CytoplasmicSequence analysisAdd
BLAST
Transmembranei57 – 7721Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini78 – 9821LumenalSequence analysisAdd
BLAST
Transmembranei99 – 11921Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini120 – 14829CytoplasmicSequence analysisAdd
BLAST
Transmembranei149 – 16921Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini170 – 18718LumenalSequence analysisAdd
BLAST
Transmembranei188 – 20821Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini209 – 26658CytoplasmicSequence analysisAdd
BLAST
Transmembranei267 – 28721Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini288 – 2936LumenalSequence analysis
Transmembranei294 – 31421Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini315 – 37460CytoplasmicSequence analysisAdd
BLAST
Transmembranei375 – 39521Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini396 – 40813LumenalSequence analysisAdd
BLAST
Transmembranei409 – 42921Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini430 – 703274CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: RGD
  • MHC class I peptide loading complex Source: RGD
  • TAP complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 703703Antigen peptide transporter 2PRO_0000093331Add
BLAST

Proteomic databases

PaxDbiP36372.
PRIDEiP36372.

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2.

Binary interactionsi

WithEntry#Exp.IntActNotes
gNQ77CE42EBI-11304494,EBI-11303846From a different organism.

GO - Molecular functioni

  • MHC class I protein binding Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi246934. 7 interactions.
IntActiP36372. 3 interactions.
STRINGi10116.ENSRNOP00000067594.

Structurei

3D structure databases

ProteinModelPortaliP36372.
SMRiP36372. Positions 457-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 435284ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini468 – 702235ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni301 – 38989Involved in peptide-binding siteBy similarityAdd
BLAST
Regioni414 – 43320Involved in peptide-binding siteBy similarityAdd
BLAST

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiP36372.
KOiK05654.
PhylomeDBiP36372.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSHPRPWA SLLLVDLALL GLLQSSLGTL LPPGLPGLWL EGTLRLGVLW
60 70 80 90 100
GLLKVGGLLR LVGTFLPLLC LTNPLFFSLR ALVGSTMSTS VVRVASASWG
110 120 130 140 150
WLLADYGAVA LSLAVWAVLS PAGAQEKEPG QENNRALMIR LLRLSKPDLP
160 170 180 190 200
FLIVAFIFLA MAVWWEMFIP HYSGRVIDIL GGDFDPDAFA SAIFFMCLFS
210 220 230 240 250
VGSSLSAGCR GGSFLFAESR INLRIREQLF SSLLRQDLAF FQETKTGELN
260 270 280 290 300
SRLSSDTSLM SQWLSLNANI LLRSLVKVVG LYYFMLQVSP RLTFLSLLDL
310 320 330 340 350
PLTIAAEKVY NPRHQAVLKE IQDAVAKAGQ VVREAVGGLQ TVRSFGAEEQ
360 370 380 390 400
EVRRYKEALE RCRQLWWRRD LEKSLYLVIQ RVMALGMQVL ILNVGVQQIL
410 420 430 440 450
AGEVTRGGLL SFLLYQEEVG HHVQNLVYMY GDMLSNVGAA EKVFSYLDRR
460 470 480 490 500
PNLPNPGTLA PPRLEGRVEF QDVSFSYPSR PEKPVLQGLT FTLHPGKVTA
510 520 530 540 550
LVGPNGSGKS TVAALLQNLY QPTGGQLLLD GEPLVQYDHH YLHRQVVLVG
560 570 580 590 600
QEPVLFSGSV KDNIAYGLRD CEDAQVMAAA QAACADDFIG EMTNGINTEI
610 620 630 640 650
GEKGSQLAVG QKQRLAIARA LVRNPRVLIL DEATSALDAE CEQALQTWRS
660 670 680 690 700
QEDRTMLVIA HRLHTVQNAD QVLVLKQGQL VEHDQLRDEQ DVYAHLVQQR

LEA
Length:703
Mass (Da):77,713
Last modified:June 1, 1994 - v1
Checksum:iE545993A8F784250
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti352 – 3521V → F in TAP2L. 1 Publication
Natural varianti603 – 6031K → R in TAP2L. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63854 mRNA. Translation: CAA45339.1.
X75305 mRNA. Translation: CAA53053.1.
PIRiS38400.
RefSeqiNP_114445.2. NM_032056.3.
UniGeneiRn.202962.

Genome annotation databases

GeneIDi24812.
KEGGirno:24812.
UCSCiRGD:3818. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63854 mRNA. Translation: CAA45339.1.
X75305 mRNA. Translation: CAA53053.1.
PIRiS38400.
RefSeqiNP_114445.2. NM_032056.3.
UniGeneiRn.202962.

3D structure databases

ProteinModelPortaliP36372.
SMRiP36372. Positions 457-698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246934. 7 interactions.
IntActiP36372. 3 interactions.
STRINGi10116.ENSRNOP00000067594.

Proteomic databases

PaxDbiP36372.
PRIDEiP36372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24812.
KEGGirno:24812.
UCSCiRGD:3818. rat.

Organism-specific databases

CTDi6891.
RGDi3818. Tap2.

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiP36372.
KOiK05654.
PhylomeDBiP36372.

Miscellaneous databases

PROiP36372.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Restoration of antigen presentation to the mutant cell line RMA-S by an MHC-linked transporter."
    Powis S.J., Townsend A.R.M., Deverson E.V., Bastin J., Butcher G.W., Howard J.C.
    Nature 354:528-531(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Effect of polymorphism of an MHC-linked transporter on the peptides assembled in a class I molecule."
    Powis S.J., Deverson E.V., Coadwell W.J., Ciruela A., Huskisson N.S., Smith H., Butcher G.W., Howard J.C.
    Nature 357:211-215(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS TAP2L PHE-352 AND ARG-603.
  3. "The distribution of Tap2 alleles among laboratory rat RT1 haplotypes."
    Joly E., Deverson E.V., Coadwell W.L., Guenther E., Howard J.C., Butcher G.W.
    Immunogenetics 40:45-53(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: PVG-RT1L(LEW).

Entry informationi

Entry nameiTAP2_RAT
AccessioniPrimary (citable) accession number: P36372
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.