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P36371 (TAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Antigen peptide transporter 2

Short name=APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Histocompatibility antigen modifier 2
Gene names
Name:Tap2
Synonyms:Abcb3, Ham-2, Ham2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin By similarity.

Subunit structure

Heterodimer of TAP1 and TAP2.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: The transmembrane segments seem to form a pore in the membrane.

Domain

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2 By similarity.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily. [View classification]

Contains 1 ABC transmembrane type-1 domain.

Contains 1 ABC transporter domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
Peptide transport
Protein transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent

Inferred from direct assay PubMed 1538753. Source: MGI

antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent

Inferred from direct assay PubMed 1538753. Source: MGI

antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Inferred from mutant phenotype PubMed 16299293. Source: MGI

oligopeptide transmembrane transport

Inferred from Biological aspect of Ancestor. Source: GOC

peptide transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of T cell mediated cytotoxicity

Inferred from direct assay PubMed 1538753. Source: MGI

positive regulation of antigen processing and presentation of peptide antigen via MHC class I

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

response to molecule of bacterial origin

Inferred from mutant phenotype PubMed 16299293. Source: MGI

transmembrane transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentTAP complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MHC class I protein binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

TAP1 binding

Inferred from physical interaction PubMed 11133832. Source: UniProtKB

TAP2 binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

oligopeptide-transporting ATPase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

peptide antigen binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein binding

Inferred from physical interaction PubMed 14735325. Source: MGI

tapasin binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 702702Antigen peptide transporter 2
PRO_0000093330

Regions

Topological domain1 – 66Lumenal Potential
Transmembrane7 – 2721Helical; Name=1; Potential
Topological domain28 – 5629Cytoplasmic Potential
Transmembrane57 – 7721Helical; Name=2; Potential
Topological domain78 – 9821Lumenal Potential
Transmembrane99 – 11921Helical; Name=3; Potential
Topological domain120 – 14728Cytoplasmic Potential
Transmembrane148 – 16821Helical; Name=4; Potential
Topological domain169 – 18618Lumenal Potential
Transmembrane187 – 20721Helical; Name=5; Potential
Topological domain208 – 26558Cytoplasmic Potential
Transmembrane266 – 28621Helical; Name=6; Potential
Topological domain287 – 2926Lumenal Potential
Transmembrane293 – 31321Helical; Name=7; Potential
Topological domain314 – 37360Cytoplasmic Potential
Transmembrane374 – 39421Helical; Name=8; Potential
Topological domain395 – 40713Lumenal Potential
Transmembrane408 – 42821Helical; Name=9; Potential
Topological domain429 – 702274Cytoplasmic Potential
Domain151 – 434284ABC transmembrane type-1
Domain467 – 701235ABC transporter
Nucleotide binding502 – 5098ATP Potential
Region300 – 38889Involved in peptide-binding site By similarity
Region413 – 43220Involved in peptide-binding site By similarity

Sequences

Sequence LengthMass (Da)Tools
P36371 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: F93DFA38B057AC0A

FASTA70277,445
        10         20         30         40         50         60 
MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW GLLKVGELLG 

        70         80         90        100        110        120 
LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG WLLAGYGAVA LSWAVWAVLS 

       130        140        150        160        170        180 
PAGVQEKEPG QENRTLMKRL LKLSRPDLPF LIAAFFFLVV AVWGETLIPR YSGRVIDILG 

       190        200        210        220        230        240 
GDFDPDAFAS AIFFMCLFSV GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF 

       250        260        270        280        290        300 
QETKTGELNS RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP 

       310        320        330        340        350        360 
LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE VSHYKEALER 

       370        380        390        400        410        420 
CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA GEVTRGGLLS FLLYQEEVGQ 

       430        440        450        460        470        480 
YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP 

       490        500        510        520        530        540 
EKPVLQGLTF TLHPGTVTAL VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY 

       550        560        570        580        590        600 
LHRQVVLVGQ EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG 

       610        620        630        640        650        660 
EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ GDRTMLVIAH 

       670        680        690        700 
RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL EA 

« Hide

References

[1]"Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules."
Yang Y., Frueh K., Chambers J., Waters J.B., Wu L., Spies T., Peterson P.A.
J. Biol. Chem. 267:11669-11672(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M90459 mRNA. Translation: AAA39609.1.
CCDSCCDS28644.1.
PIRA44135.
RefSeqNP_035660.3. NM_011530.3.
XP_006524055.1. XM_006523992.1.
XP_006525418.1. XM_006525355.1.
UniGeneMm.14814.

3D structure databases

ProteinModelPortalP36371.
SMRP36371. Positions 138-697.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP36371. 1 interaction.
MINTMINT-4136884.
STRING10090.ENSMUSP00000025197.

PTM databases

PhosphoSiteP36371.

Proteomic databases

PaxDbP36371.
PRIDEP36371.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
GeneID21355.
KEGGmmu:21355.
UCSCuc008cbx.2. mouse.

Organism-specific databases

CTD6891.
MGIMGI:98484. Tap2.

Phylogenomic databases

eggNOGCOG1132.
HOVERGENHBG008358.
InParanoidP36371.
KOK05654.
OMAVGLYSFM.
OrthoDBEOG7Z3F4H.
PhylomeDBP36371.
TreeFamTF105197.

Gene expression databases

ArrayExpressP36371.
BgeeP36371.
GenevestigatorP36371.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013306. ABC_B3.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR005293. Ag_transporter2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSPR01897. TAP2PROTEIN.
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsTIGR00958. 3a01208. 1 hit.
PROSITEPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300556.
PROP36371.
SOURCESearch...

Entry information

Entry nameTAP2_MOUSE
AccessionPrimary (citable) accession number: P36371
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot