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Protein

Antigen peptide transporter 2

Gene

Tap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi502 – 5098ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity, coupled to transmembrane movement of substances Source: GO_Central
  2. ATP binding Source: MGI
  3. MHC class Ib protein binding Source: MGI
  4. peptide antigen-transporting ATPase activity Source: MGI
  5. TAP1 binding Source: UniProtKB
  6. tapasin binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation of endogenous peptide antigen via MHC class I Source: MGI
  2. antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent Source: MGI
  3. antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent Source: MGI
  4. antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Source: MGI
  5. cytosol to ER transport Source: MGI
  6. intracellular transport of viral protein in host cell Source: MGI
  7. peptide antigen transport Source: MGI
  8. positive regulation of antigen processing and presentation of peptide antigen via MHC class I Source: GO_Central
  9. positive regulation of T cell mediated cytotoxicity Source: MGI
  10. response to molecule of bacterial origin Source: MGI
  11. transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_339401. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.
REACT_341075. ER-Phagosome pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 2
Short name:
APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Histocompatibility antigen modifier 2
Gene namesi
Name:Tap2
Synonyms:Abcb3, Ham-2, Ham2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98484. Tap2.

Subcellular locationi

  1. Endoplasmic reticulum membrane; Multi-pass membrane protein

  2. Note: The transmembrane segments seem to form a pore in the membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66LumenalSequence Analysis
Transmembranei7 – 2721Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini28 – 5629CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei57 – 7721Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini78 – 9821LumenalSequence AnalysisAdd
BLAST
Transmembranei99 – 11921Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini120 – 14728CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei148 – 16821Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini169 – 18618LumenalSequence AnalysisAdd
BLAST
Transmembranei187 – 20721Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini208 – 26558CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei266 – 28621Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini287 – 2926LumenalSequence Analysis
Transmembranei293 – 31321Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini314 – 37360CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei374 – 39421Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini395 – 40713LumenalSequence AnalysisAdd
BLAST
Transmembranei408 – 42821Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini429 – 702274CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: MGI
  2. integral component of membrane Source: MGI
  3. membrane Source: MGI
  4. TAP complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Antigen peptide transporter 2PRO_0000093330Add
BLAST

Proteomic databases

MaxQBiP36371.
PaxDbiP36371.
PRIDEiP36371.

PTM databases

PhosphoSiteiP36371.

Expressioni

Gene expression databases

BgeeiP36371.
ExpressionAtlasiP36371. baseline and differential.
GenevestigatoriP36371.

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2.

Protein-protein interaction databases

IntActiP36371. 1 interaction.
MINTiMINT-4136884.
STRINGi10090.ENSMUSP00000025197.

Structurei

3D structure databases

ProteinModelPortaliP36371.
SMRiP36371. Positions 138-697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini151 – 434284ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini467 – 701235ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 38889Involved in peptide-binding siteBy similarityAdd
BLAST
Regioni413 – 43220Involved in peptide-binding siteBy similarityAdd
BLAST

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
HOVERGENiHBG008358.
InParanoidiP36371.
KOiK05654.
OMAiVGLYSFM.
OrthoDBiEOG7Z3F4H.
PhylomeDBiP36371.
TreeFamiTF105197.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PANTHERiPTHR24221:SF160. PTHR24221:SF160. 1 hit.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW
60 70 80 90 100
GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG
110 120 130 140 150
WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF
160 170 180 190 200
LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV
210 220 230 240 250
GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS
260 270 280 290 300
RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP
310 320 330 340 350
LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE
360 370 380 390 400
VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA
410 420 430 440 450
GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP
460 470 480 490 500
NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL
510 520 530 540 550
VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ
560 570 580 590 600
EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG
610 620 630 640 650
EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ
660 670 680 690 700
GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL

EA
Length:702
Mass (Da):77,445
Last modified:June 1, 1994 - v1
Checksum:iF93DFA38B057AC0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90459 mRNA. Translation: AAA39609.1.
CCDSiCCDS28644.1.
PIRiA44135.
RefSeqiNP_035660.3. NM_011530.3.
XP_006524055.1. XM_006523992.1.
XP_006525418.1. XM_006525355.1.
UniGeneiMm.14814.

Genome annotation databases

EnsembliENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
GeneIDi21355.
KEGGimmu:21355.
UCSCiuc008cbx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90459 mRNA. Translation: AAA39609.1.
CCDSiCCDS28644.1.
PIRiA44135.
RefSeqiNP_035660.3. NM_011530.3.
XP_006524055.1. XM_006523992.1.
XP_006525418.1. XM_006525355.1.
UniGeneiMm.14814.

3D structure databases

ProteinModelPortaliP36371.
SMRiP36371. Positions 138-697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP36371. 1 interaction.
MINTiMINT-4136884.
STRINGi10090.ENSMUSP00000025197.

PTM databases

PhosphoSiteiP36371.

Proteomic databases

MaxQBiP36371.
PaxDbiP36371.
PRIDEiP36371.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
GeneIDi21355.
KEGGimmu:21355.
UCSCiuc008cbx.2. mouse.

Organism-specific databases

CTDi6891.
MGIiMGI:98484. Tap2.

Phylogenomic databases

eggNOGiCOG1132.
HOVERGENiHBG008358.
InParanoidiP36371.
KOiK05654.
OMAiVGLYSFM.
OrthoDBiEOG7Z3F4H.
PhylomeDBiP36371.
TreeFamiTF105197.

Enzyme and pathway databases

ReactomeiREACT_339401. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.
REACT_341075. ER-Phagosome pathway.

Miscellaneous databases

NextBioi300556.
PROiP36371.
SOURCEiSearch...

Gene expression databases

BgeeiP36371.
ExpressionAtlasiP36371. baseline and differential.
GenevestigatoriP36371.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PANTHERiPTHR24221:SF160. PTHR24221:SF160. 1 hit.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules."
    Yang Y., Frueh K., Chambers J., Waters J.B., Wu L., Spies T., Peterson P.A.
    J. Biol. Chem. 267:11669-11672(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTAP2_MOUSE
AccessioniPrimary (citable) accession number: P36371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 1, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.