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P36371

- TAP2_MOUSE

UniProt

P36371 - TAP2_MOUSE

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Protein

Antigen peptide transporter 2

Gene
Tap2, Abcb3, Ham-2, Ham2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi502 – 5098ATP Reviewed prediction

GO - Molecular functioni

  1. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
  2. ATP binding Source: UniProtKB-KW
  3. peptide transporter activity Source: InterPro
  4. protein binding Source: MGI
  5. TAP1 binding Source: UniProtKB
  6. tapasin binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent Source: MGI
  2. antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent Source: MGI
  3. antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Source: MGI
  4. positive regulation of antigen processing and presentation of peptide antigen via MHC class I Source: RefGenome
  5. positive regulation of T cell mediated cytotoxicity Source: MGI
  6. protein transport Source: UniProtKB-KW
  7. response to molecule of bacterial origin Source: MGI
  8. transmembrane transport Source: RefGenome
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_199105. ER-Phagosome pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 2
Short name:
APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Histocompatibility antigen modifier 2
Gene namesi
Name:Tap2
Synonyms:Abcb3, Ham-2, Ham2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:98484. Tap2.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein
Note: The transmembrane segments seem to form a pore in the membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Lumenal Reviewed prediction
Transmembranei7 – 2721Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini28 – 5629Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei57 – 7721Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini78 – 9821Lumenal Reviewed predictionAdd
BLAST
Transmembranei99 – 11921Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini120 – 14728Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei148 – 16821Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini169 – 18618Lumenal Reviewed predictionAdd
BLAST
Transmembranei187 – 20721Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini208 – 26558Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei266 – 28621Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini287 – 2926Lumenal Reviewed prediction
Transmembranei293 – 31321Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini314 – 37360Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei374 – 39421Helical; Name=8; Reviewed predictionAdd
BLAST
Topological domaini395 – 40713Lumenal Reviewed predictionAdd
BLAST
Transmembranei408 – 42821Helical; Name=9; Reviewed predictionAdd
BLAST
Topological domaini429 – 702274Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Antigen peptide transporter 2PRO_0000093330Add
BLAST

Proteomic databases

PaxDbiP36371.
PRIDEiP36371.

PTM databases

PhosphoSiteiP36371.

Expressioni

Gene expression databases

ArrayExpressiP36371.
BgeeiP36371.
GenevestigatoriP36371.

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2.

Protein-protein interaction databases

IntActiP36371. 1 interaction.
MINTiMINT-4136884.
STRINGi10090.ENSMUSP00000025197.

Structurei

3D structure databases

ProteinModelPortaliP36371.
SMRiP36371. Positions 138-697.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini151 – 434284ABC transmembrane type-1Add
BLAST
Domaini467 – 701235ABC transporterAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 38889Involved in peptide-binding site By similarityAdd
BLAST
Regioni413 – 43220Involved in peptide-binding site By similarityAdd
BLAST

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2 By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
HOVERGENiHBG008358.
InParanoidiP36371.
KOiK05654.
OMAiVGLYSFM.
OrthoDBiEOG7Z3F4H.
PhylomeDBiP36371.
TreeFamiTF105197.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013306. ABC_B3.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR005293. Ag_transporter2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36371-1 [UniParc]FASTAAdd to Basket

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MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW    50
GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG 100
WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF 150
LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV 200
GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS 250
RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP 300
LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE 350
VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA 400
GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP 450
NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL 500
VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ 550
EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG 600
EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ 650
GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL 700
EA 702
Length:702
Mass (Da):77,445
Last modified:June 1, 1994 - v1
Checksum:iF93DFA38B057AC0A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90459 mRNA. Translation: AAA39609.1.
CCDSiCCDS28644.1.
PIRiA44135.
RefSeqiNP_035660.3. NM_011530.3.
XP_006524055.1. XM_006523992.1.
XP_006525418.1. XM_006525355.1.
UniGeneiMm.14814.

Genome annotation databases

EnsembliENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
GeneIDi21355.
KEGGimmu:21355.
UCSCiuc008cbx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90459 mRNA. Translation: AAA39609.1 .
CCDSi CCDS28644.1.
PIRi A44135.
RefSeqi NP_035660.3. NM_011530.3.
XP_006524055.1. XM_006523992.1.
XP_006525418.1. XM_006525355.1.
UniGenei Mm.14814.

3D structure databases

ProteinModelPortali P36371.
SMRi P36371. Positions 138-697.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P36371. 1 interaction.
MINTi MINT-4136884.
STRINGi 10090.ENSMUSP00000025197.

PTM databases

PhosphoSitei P36371.

Proteomic databases

PaxDbi P36371.
PRIDEi P36371.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025197 ; ENSMUSP00000025197 ; ENSMUSG00000024339 .
GeneIDi 21355.
KEGGi mmu:21355.
UCSCi uc008cbx.2. mouse.

Organism-specific databases

CTDi 6891.
MGIi MGI:98484. Tap2.

Phylogenomic databases

eggNOGi COG1132.
HOVERGENi HBG008358.
InParanoidi P36371.
KOi K05654.
OMAi VGLYSFM.
OrthoDBi EOG7Z3F4H.
PhylomeDBi P36371.
TreeFami TF105197.

Enzyme and pathway databases

Reactomei REACT_199105. ER-Phagosome pathway.

Miscellaneous databases

NextBioi 300556.
PROi P36371.
SOURCEi Search...

Gene expression databases

ArrayExpressi P36371.
Bgeei P36371.
Genevestigatori P36371.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013306. ABC_B3.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR005293. Ag_transporter2.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view ]
PRINTSi PR01897. TAP2PROTEIN.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsi TIGR00958. 3a01208. 1 hit.
PROSITEi PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules."
    Yang Y., Frueh K., Chambers J., Waters J.B., Wu L., Spies T., Peterson P.A.
    J. Biol. Chem. 267:11669-11672(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTAP2_MOUSE
AccessioniPrimary (citable) accession number: P36371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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