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P36371

- TAP2_MOUSE

UniProt

P36371 - TAP2_MOUSE

Protein

Antigen peptide transporter 2

Gene

Tap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi502 – 5098ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
    2. ATP binding Source: UniProtKB-KW
    3. peptide transporter activity Source: InterPro
    4. protein binding Source: MGI
    5. TAP1 binding Source: UniProtKB
    6. tapasin binding Source: UniProtKB

    GO - Biological processi

    1. antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent Source: MGI
    2. antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent Source: MGI
    3. antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Source: MGI
    4. positive regulation of antigen processing and presentation of peptide antigen via MHC class I Source: RefGenome
    5. positive regulation of T cell mediated cytotoxicity Source: MGI
    6. protein transport Source: UniProtKB-KW
    7. response to molecule of bacterial origin Source: MGI
    8. transmembrane transport Source: RefGenome

    Keywords - Biological processi

    Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_199105. ER-Phagosome pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Antigen peptide transporter 2
    Short name:
    APT2
    Alternative name(s):
    ATP-binding cassette sub-family B member 3
    Histocompatibility antigen modifier 2
    Gene namesi
    Name:Tap2
    Synonyms:Abcb3, Ham-2, Ham2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98484. Tap2.

    Subcellular locationi

    Endoplasmic reticulum membrane; Multi-pass membrane protein
    Note: The transmembrane segments seem to form a pore in the membrane.

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: RefGenome

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 702702Antigen peptide transporter 2PRO_0000093330Add
    BLAST

    Proteomic databases

    PaxDbiP36371.
    PRIDEiP36371.

    PTM databases

    PhosphoSiteiP36371.

    Expressioni

    Gene expression databases

    ArrayExpressiP36371.
    BgeeiP36371.
    GenevestigatoriP36371.

    Interactioni

    Subunit structurei

    Heterodimer of TAP1 and TAP2.

    Protein-protein interaction databases

    IntActiP36371. 1 interaction.
    MINTiMINT-4136884.
    STRINGi10090.ENSMUSP00000025197.

    Structurei

    3D structure databases

    ProteinModelPortaliP36371.
    SMRiP36371. Positions 138-697.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66LumenalSequence Analysis
    Topological domaini28 – 5629CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini78 – 9821LumenalSequence AnalysisAdd
    BLAST
    Topological domaini120 – 14728CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini169 – 18618LumenalSequence AnalysisAdd
    BLAST
    Topological domaini208 – 26558CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini287 – 2926LumenalSequence Analysis
    Topological domaini314 – 37360CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini395 – 40713LumenalSequence AnalysisAdd
    BLAST
    Topological domaini429 – 702274CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Name=1PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei57 – 7721Helical; Name=2PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei99 – 11921Helical; Name=3PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei148 – 16821Helical; Name=4PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei187 – 20721Helical; Name=5PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei266 – 28621Helical; Name=6PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei293 – 31321Helical; Name=7PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei374 – 39421Helical; Name=8PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei408 – 42821Helical; Name=9PROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini151 – 434284ABC transmembrane type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini467 – 701235ABC transporterPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni300 – 38889Involved in peptide-binding siteBy similarityAdd
    BLAST
    Regioni413 – 43220Involved in peptide-binding siteBy similarityAdd
    BLAST

    Domaini

    The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

    Sequence similaritiesi

    Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1132.
    HOVERGENiHBG008358.
    InParanoidiP36371.
    KOiK05654.
    OMAiVGLYSFM.
    OrthoDBiEOG7Z3F4H.
    PhylomeDBiP36371.
    TreeFamiTF105197.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR013306. ABC_B3.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR005293. Ag_transporter2.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view]
    PRINTSiPR01897. TAP2PROTEIN.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    TIGRFAMsiTIGR00958. 3a01208. 1 hit.
    PROSITEiPS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36371-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW    50
    GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG 100
    WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF 150
    LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV 200
    GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS 250
    RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP 300
    LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE 350
    VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA 400
    GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP 450
    NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL 500
    VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ 550
    EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG 600
    EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ 650
    GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL 700
    EA 702
    Length:702
    Mass (Da):77,445
    Last modified:June 1, 1994 - v1
    Checksum:iF93DFA38B057AC0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90459 mRNA. Translation: AAA39609.1.
    CCDSiCCDS28644.1.
    PIRiA44135.
    RefSeqiNP_035660.3. NM_011530.3.
    XP_006524055.1. XM_006523992.1.
    XP_006525418.1. XM_006525355.1.
    UniGeneiMm.14814.

    Genome annotation databases

    EnsembliENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
    GeneIDi21355.
    KEGGimmu:21355.
    UCSCiuc008cbx.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90459 mRNA. Translation: AAA39609.1 .
    CCDSi CCDS28644.1.
    PIRi A44135.
    RefSeqi NP_035660.3. NM_011530.3.
    XP_006524055.1. XM_006523992.1.
    XP_006525418.1. XM_006525355.1.
    UniGenei Mm.14814.

    3D structure databases

    ProteinModelPortali P36371.
    SMRi P36371. Positions 138-697.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P36371. 1 interaction.
    MINTi MINT-4136884.
    STRINGi 10090.ENSMUSP00000025197.

    PTM databases

    PhosphoSitei P36371.

    Proteomic databases

    PaxDbi P36371.
    PRIDEi P36371.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025197 ; ENSMUSP00000025197 ; ENSMUSG00000024339 .
    GeneIDi 21355.
    KEGGi mmu:21355.
    UCSCi uc008cbx.2. mouse.

    Organism-specific databases

    CTDi 6891.
    MGIi MGI:98484. Tap2.

    Phylogenomic databases

    eggNOGi COG1132.
    HOVERGENi HBG008358.
    InParanoidi P36371.
    KOi K05654.
    OMAi VGLYSFM.
    OrthoDBi EOG7Z3F4H.
    PhylomeDBi P36371.
    TreeFami TF105197.

    Enzyme and pathway databases

    Reactomei REACT_199105. ER-Phagosome pathway.

    Miscellaneous databases

    NextBioi 300556.
    PROi P36371.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P36371.
    Bgeei P36371.
    Genevestigatori P36371.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR013306. ABC_B3.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR005293. Ag_transporter2.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    PRINTSi PR01897. TAP2PROTEIN.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    TIGRFAMsi TIGR00958. 3a01208. 1 hit.
    PROSITEi PS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Major histocompatibility complex (MHC)-encoded HAM2 is necessary for antigenic peptide loading onto class I MHC molecules."
      Yang Y., Frueh K., Chambers J., Waters J.B., Wu L., Spies T., Peterson P.A.
      J. Biol. Chem. 267:11669-11672(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiTAP2_MOUSE
    AccessioniPrimary (citable) accession number: P36371
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3