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Protein

Antigen peptide transporter 2

Gene

Tap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi502 – 509ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 2
Short name:
APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Histocompatibility antigen modifier 2
Gene namesi
Name:Tap2
Synonyms:Abcb3, Ham-2, Ham2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98484. Tap2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalSequence analysis6
Transmembranei7 – 27Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini28 – 56CytoplasmicSequence analysisAdd BLAST29
Transmembranei57 – 77Helical; Name=2PROSITE-ProRule annotationAdd BLAST21
Topological domaini78 – 98LumenalSequence analysisAdd BLAST21
Transmembranei99 – 119Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini120 – 147CytoplasmicSequence analysisAdd BLAST28
Transmembranei148 – 168Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini169 – 186LumenalSequence analysisAdd BLAST18
Transmembranei187 – 207Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini208 – 265CytoplasmicSequence analysisAdd BLAST58
Transmembranei266 – 286Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini287 – 292LumenalSequence analysis6
Transmembranei293 – 313Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini314 – 373CytoplasmicSequence analysisAdd BLAST60
Transmembranei374 – 394Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini395 – 407LumenalSequence analysisAdd BLAST13
Transmembranei408 – 428Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini429 – 702CytoplasmicSequence analysisAdd BLAST274

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933301 – 702Antigen peptide transporter 2Add BLAST702

Proteomic databases

EPDiP36371.
PaxDbiP36371.
PeptideAtlasiP36371.
PRIDEiP36371.

PTM databases

iPTMnetiP36371.
PhosphoSitePlusiP36371.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024339.
ExpressionAtlasiP36371. baseline and differential.
GenevisibleiP36371. MM.

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2.

GO - Molecular functioni

  • MHC class Ib protein binding Source: MGI
  • TAP1 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

Protein-protein interaction databases

IntActiP36371. 2 interactors.
MINTiMINT-4136884.
STRINGi10090.ENSMUSP00000025197.

Structurei

3D structure databases

ProteinModelPortaliP36371.
SMRiP36371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini151 – 434ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini467 – 701ABC transporterPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni300 – 388Involved in peptide-binding siteBy similarityAdd BLAST89
Regioni413 – 432Involved in peptide-binding siteBy similarityAdd BLAST20

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00550000074497.
HOVERGENiHBG008358.
InParanoidiP36371.
KOiK05654.
OMAiYNTRHQA.
OrthoDBiEOG091G05Q7.
PhylomeDBiP36371.
TreeFamiTF105197.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW
60 70 80 90 100
GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG
110 120 130 140 150
WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF
160 170 180 190 200
LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV
210 220 230 240 250
GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS
260 270 280 290 300
RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP
310 320 330 340 350
LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE
360 370 380 390 400
VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA
410 420 430 440 450
GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP
460 470 480 490 500
NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL
510 520 530 540 550
VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ
560 570 580 590 600
EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG
610 620 630 640 650
EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ
660 670 680 690 700
GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL

EA
Length:702
Mass (Da):77,445
Last modified:June 1, 1994 - v1
Checksum:iF93DFA38B057AC0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90459 mRNA. Translation: AAA39609.1.
CCDSiCCDS28644.1.
PIRiA44135.
RefSeqiNP_035660.3. NM_011530.3.
UniGeneiMm.14814.

Genome annotation databases

EnsembliENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
GeneIDi21355.
KEGGimmu:21355.
UCSCiuc008cbx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90459 mRNA. Translation: AAA39609.1.
CCDSiCCDS28644.1.
PIRiA44135.
RefSeqiNP_035660.3. NM_011530.3.
UniGeneiMm.14814.

3D structure databases

ProteinModelPortaliP36371.
SMRiP36371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP36371. 2 interactors.
MINTiMINT-4136884.
STRINGi10090.ENSMUSP00000025197.

PTM databases

iPTMnetiP36371.
PhosphoSitePlusiP36371.

Proteomic databases

EPDiP36371.
PaxDbiP36371.
PeptideAtlasiP36371.
PRIDEiP36371.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025197; ENSMUSP00000025197; ENSMUSG00000024339.
GeneIDi21355.
KEGGimmu:21355.
UCSCiuc008cbx.2. mouse.

Organism-specific databases

CTDi6891.
MGIiMGI:98484. Tap2.

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00550000074497.
HOVERGENiHBG008358.
InParanoidiP36371.
KOiK05654.
OMAiYNTRHQA.
OrthoDBiEOG091G05Q7.
PhylomeDBiP36371.
TreeFamiTF105197.

Enzyme and pathway databases

ReactomeiR-MMU-1236974. ER-Phagosome pathway.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

PROiP36371.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024339.
ExpressionAtlasiP36371. baseline and differential.
GenevisibleiP36371. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005293. Tap2/ABCB3.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSiPR01897. TAP2PROTEIN.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAP2_MOUSE
AccessioniPrimary (citable) accession number: P36371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.