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Protein

Antigen peptide transporter 1

Gene

Tap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi515 – 5228ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ADP binding Source: RGD
  • ATPase activity Source: RGD
  • ATP binding Source: UniProtKB
  • MHC class I protein binding Source: UniProtKB
  • nucleotide binding Source: RGD
  • peptide antigen-transporting ATPase activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation of endogenous peptide antigen via MHC class I Source: InterPro
  • peptide antigen transport Source: RGD
  • peptide transport Source: RGD
  • protection from natural killer cell mediated cytotoxicity Source: RGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 1
Short name:
APT1
Alternative name(s):
ATP-binding cassette sub-family B member 2
Peptide transporter TAP1
Gene namesi
Name:Tap1
Synonyms:Abcb2, Mtp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3817. Tap1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini30 – 389LumenalSequence analysis
Transmembranei39 – 6022Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini61 – 677CytoplasmicSequence analysis
Transmembranei68 – 8821Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini89 – 11022LumenalSequence analysisAdd
BLAST
Transmembranei111 – 13121Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini132 – 16332CytoplasmicSequence analysisAdd
BLAST
Transmembranei164 – 18421Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini185 – 20420LumenalSequence analysisAdd
BLAST
Transmembranei205 – 22521Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini226 – 27550CytoplasmicSequence analysisAdd
BLAST
Transmembranei276 – 29621Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini297 – 3059LumenalSequence analysis
Transmembranei306 – 32621Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini327 – 39569CytoplasmicSequence analysisAdd
BLAST
Transmembranei396 – 41621Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini417 – 4204LumenalSequence analysis
Transmembranei421 – 44121Helical; Name=10PROSITE-ProRule annotationAdd
BLAST
Topological domaini442 – 725284CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: RGD
  • TAP complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Antigen peptide transporter 1PRO_0000093328Add
BLAST

Proteomic databases

PaxDbiP36370.
PRIDEiP36370.

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2. Interacts with PSMB5 and PSMB8 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TapbpQ99JC62EBI-11303917,EBI-11304538

GO - Molecular functioni

  • MHC class I protein binding Source: UniProtKB
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • TAP2 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

Protein-protein interaction databases

IntActiP36370. 3 interactions.
STRINGi10116.ENSRNOP00000000529.

Structurei

Secondary structure

1
725
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni466 – 4694Combined sources
Beta strandi480 – 4878Combined sources
Beta strandi498 – 5058Combined sources
Beta strandi510 – 5145Combined sources
Helixi521 – 5288Combined sources
Beta strandi535 – 5417Combined sources
Helixi546 – 5483Combined sources
Helixi551 – 5577Combined sources
Beta strandi558 – 5614Combined sources
Beta strandi569 – 5713Combined sources
Helixi572 – 5776Combined sources
Helixi586 – 59510Combined sources
Helixi599 – 6046Combined sources
Helixi608 – 6103Combined sources
Helixi615 – 6173Combined sources
Helixi622 – 63413Combined sources
Beta strandi639 – 6457Combined sources
Turni646 – 6494Combined sources
Helixi652 – 66413Combined sources
Turni666 – 6705Combined sources
Beta strandi671 – 6766Combined sources
Helixi680 – 6834Combined sources
Beta strandi687 – 6937Combined sources
Beta strandi696 – 7016Combined sources
Helixi703 – 7097Combined sources
Helixi712 – 7187Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IXEX-ray2.00A/D465-725[»]
2IXFX-ray2.00A/B/C/D465-725[»]
2IXGX-ray2.70A465-725[»]
4K8OX-ray2.65A465-725[»]
ProteinModelPortaliP36370.
SMRiP36370. Positions 465-719.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36370.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini164 – 447284ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini480 – 719240ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni352 – 39746Involved in peptide-binding siteBy similarityAdd
BLAST
Regioni430 – 46435Involved in peptide-binding siteBy similarityAdd
BLAST

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiP36370.
PhylomeDBiP36370.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR013306. Tap1/ABCB2.
[Graphical view]
PANTHERiPTHR24221:SF249. PTHR24221:SF249. 2 hits.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAHAWPTAA LLLLLVDWLL LRPVLPGIFS LLVPEVPLLR VWAVGLSRWA
60 70 80 90 100
ILGLGVRGVL GVTAGARGWL AALQPLVAAL GLALPGLASF RKLSAWGALR
110 120 130 140 150
EGDNAGLLHW NSRLDAFVLS YVAALPAAAL WHKLGGFWAP SGHKGAGDML
160 170 180 190 200
CRMLGFLDSK KGRLHLVLVL LILSCLGEMA IPFFTGRITD WILQDKTAPS
210 220 230 240 250
FARNMWLMCI LTIASTVLEF AGDGIYNITM GHMHSRVHGE VFRAVLHQET
260 270 280 290 300
GFFLKNPTGS ITSRVTEDTS NVCESISDKL NLFLWYLGRG LCLLAFMIWG
310 320 330 340 350
SFYLTVVTLL SLPLLFLLPR RLGKVYQSLA VKVQESLAKS TQVALEALSA
360 370 380 390 400
MPTVRSFANE EGEAQKFRQK LEEMKPLNKK EALAYVTEVW TMSVSGMLLK
410 420 430 440 450
VGILYLGGQL VVRGAVSSGN LVSFVLYQLQ FTRAVEVLLS IYPSMQKSVG
460 470 480 490 500
ASEKIFEYLD RTPCSPLSGS LAPLNMKGLV KFQDVSFAYP NHPNVQVLQG
510 520 530 540 550
LTFTLYPGKV TALVGPNGSG KSTVAALLQN LYQPTGGKVL LDGEPLVQYD
560 570 580 590 600
HHYLHTQVAA VGQEPLLFGR SFRENIAYGL TRTPTMEEIT AVAMESGAHD
610 620 630 640 650
FISGFPQGYD TEVGETGNQL SGGQRQAVAL ARALIRKPRL LILDDATSAL
660 670 680 690 700
DAGNQLRVQR LLYESPEWAS RTVLLITQQL SLAERAHHIL FLKEGSVCEQ
710 720
GTHLQLMERG GCYRSMVEAL AAPSD
Length:725
Mass (Da):79,150
Last modified:November 1, 1997 - v2
Checksum:i3FA7215D0AC22EE0
GO

Sequence cautioni

The sequence CAA40742.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57523 mRNA. Translation: CAA40742.1. Different initiation.
Y10231 mRNA. Translation: CAA71280.1.
PIRiS13426.
UniGeneiRn.10763.

Genome annotation databases

UCSCiRGD:3817. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57523 mRNA. Translation: CAA40742.1. Different initiation.
Y10231 mRNA. Translation: CAA71280.1.
PIRiS13426.
UniGeneiRn.10763.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IXEX-ray2.00A/D465-725[»]
2IXFX-ray2.00A/B/C/D465-725[»]
2IXGX-ray2.70A465-725[»]
4K8OX-ray2.65A465-725[»]
ProteinModelPortaliP36370.
SMRiP36370. Positions 465-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP36370. 3 interactions.
STRINGi10116.ENSRNOP00000000529.

Proteomic databases

PaxDbiP36370.
PRIDEiP36370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3817. rat.

Organism-specific databases

RGDi3817. Tap1.

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiP36370.
PhylomeDBiP36370.

Miscellaneous databases

EvolutionaryTraceiP36370.
PROiP36370.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_Tap-like.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR013306. Tap1/ABCB2.
[Graphical view]
PANTHERiPTHR24221:SF249. PTHR24221:SF249. 2 hits.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00958. 3a01208. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "MHC class II region encoding proteins related to the multidrug resistance family of transmembrane transporters."
    Deverson E.V., Gow I.R., Coadwell W.J., Monaco J.J., Butcher G.W., Howard J.C.
    Nature 348:738-741(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Deverson E.V.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BDIX.
    Tissue: Lymphocyte.

Entry informationi

Entry nameiTAP1_RAT
AccessioniPrimary (citable) accession number: P36370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.