P36369 (K1B26_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein 1-related peptidase b26
EC=3.4.21.35

Alternative name(s):
Glandular kallikrein K26
Short name=mGK-26
Prorenin-converting enzyme 2
Short name=PRECE-2
Tissue kallikrein 26

Gene names

Name:	Klk1b26
Synonyms:	Klk-26, Klk26

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Prorenin-converting enzyme cleaves mouse REN-2 prorenin at a dibasic site to yield mature renin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain renin-angiotensin system Inferred from direct assay PubMed 16585392. Source: MGI proteolysis Inferred from electronic annotation. Source: UniProtKB-KW zymogen activation Inferred from direct assay Ref.1PubMed 9507064. Source: MGI
Cellular_component	extracellular space Inferred from direct assay Ref.1. Source: MGI
Molecular_function	peptidase activity Inferred from direct assay Ref.1PubMed 9507064. Source: MGI serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide		PRO_0000027993
	Chain	25 – 261	237	Kallikrein 1-related peptidase b26		PRO_0000027994
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity
Experimental info
	Sequence conflict	114 – 115	2	GA → EY Ref.4
	Sequence conflict	117	1	F → Y Ref.4
	Sequence conflict	144	1	K → E in CAA24211. Ref.4
	Sequence conflict	148	1	P → L in CAA24211. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36369 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 55DCE378F4B007C5	FASTA	261	28,463
	10 20 30 40 50 60 MWFLILFPAL SLGGIDAAPP LQSRVVGGFN CEKNSQPWQV AVYYQKEHIC GGVLLDRNWV 70 80 90 100 110 120 LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG FNMSLLMLQT TPPGADFSND 130 140 150 160 170 180 LMLLRLSKPA DITDVVKPIA LPTKEPKPGS TCLASGWGSI TPTRWQKSDD LQCVFITLLP 190 200 210 220 230 240 NENCAKVYLQ KVTDVMLCAG EMGGGKDTCA GDSGGPLICD GILQGTTSNG PEPCGKPGVP 250 260 AIYTNLIKFN SWIKDTMMKN A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The presence of two types of prorenin converting enzymes in the mouse submandibular gland." Kim W.S., Nakayama K., Murakami K. FEBS Lett. 293:142-144(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Submandibular gland.
[2]	"Sequence of an epidermal growth factor-binding protein." Lundgren S., Ronne H., Rask L., Peterson P.A. J. Biol. Chem. 259:7780-7784(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NMRI.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Brain and Salivary gland.
[4]	"Mouse glandular kallikrein genes. Nucleotide sequence of cloned cDNA coding for a member of the kallikrein arginyl esteropeptidase group of serine proteases." Richards R.I., Catanzaro D.F., Mason A.J., Morris B.J., Baxter J.D., Shine J. J. Biol. Chem. 257:2758-2761(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-261. Strain: Quakenbush inbred.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01831 mRNA. Translation: AAA37540.1. X63327 mRNA. Translation: CAA44931.1. BC024688 mRNA. Translation: AAH24688.1. BC138704 mRNA. Translation: AAI38705.1. V00828 mRNA. Translation: CAA24211.1.
IPI	IPI00121821.
PIR	EGMSB. A00940.
RefSeq	NP_034774.1. NM_010644.2.
UniGene	Mm.439663.
3D structure databases
ProteinModelPortal	P36369.
SMR	P36369. Positions 25-261.
ModBase	Search...
Protein family/group databases
MEROPS	S01.070.
Proteomic databases
PRIDE	P36369.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048945; ENSMUSP00000047488; ENSMUSG00000053719.
GeneID	16618.
KEGG	mmu:16618.
UCSC	uc009gof.2. mouse.
Organism-specific databases
CTD	16618.
MGI	MGI:891981. Klk1b26.
Phylogenomic databases
GeneTree	ENSGT00690000101721.
HOVERGEN	HBG013304.
InParanoid	P36369.
KO	K01325.
OMA	NENCAKV.
Gene expression databases
Bgee	P36369.
CleanEx	MM_KLK1B26.
Genevestigator	P36369.
GermOnline	ENSMUSG00000053719. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Klk1b26. mouse.
NextBio	290219.
SOURCE	Search...

Entry information

Entry name

K1B26_MOUSE

Accession

Primary (citable) accession number: P36369
Secondary accession number(s): B9EI06, P00753, P00754

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program