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P36368

- EGFB2_MOUSE

UniProt

P36368 - EGFB2_MOUSE

Protein

Epidermal growth factor-binding protein type B

Gene

Egfbp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Cleaves REN2 at a dibasic site to yield mature renin.

    Catalytic activityi

    Hydrolyzes mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-|- pair in the N-terminus, to yield mature renin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay system
    Active sitei120 – 1201Charge relay system
    Active sitei213 – 2131Charge relay system

    GO - Molecular functioni

    1. peptidase activity Source: MGI
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. zymogen activation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BRENDAi3.4.21.119. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epidermal growth factor-binding protein type B (EC:3.4.21.119)
    Short name:
    EGF-BP B
    Alternative name(s):
    Glandular kallikrein K13
    Short name:
    mGK-13
    Prorenin-converting enzyme 1
    Short name:
    PRECE-1
    Tissue kallikrein 13
    Gene namesi
    Name:Egfbp2
    Synonyms:Egfbp-2, Klk-13, Klk13
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:95292. Egfbp2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818CuratedAdd
    BLAST
    Propeptidei19 – 246Activation peptidePRO_0000027983
    Chaini25 – 261237Epidermal growth factor-binding protein type BPRO_0000027984Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 173
    Disulfide bondi50 ↔ 66
    Glycosylationi102 – 1021N-linked (GlcNAc...)
    Disulfide bondi152 ↔ 219
    Disulfide bondi184 ↔ 198
    Disulfide bondi209 ↔ 234

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP36368.
    PRIDEiP36368.

    PTM databases

    PhosphoSiteiP36368.

    Expressioni

    Gene expression databases

    GenevestigatoriP36368.

    Structurei

    Secondary structure

    1
    261
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi39 – 446
    Beta strandi47 – 5610
    Beta strandi59 – 624
    Beta strandi72 – 765
    Beta strandi79 – 824
    Beta strandi92 – 976
    Helixi103 – 1075
    Beta strandi122 – 1287
    Beta strandi134 – 1363
    Beta strandi151 – 1577
    Beta strandi172 – 1798
    Helixi181 – 1877
    Beta strandi196 – 2005
    Beta strandi216 – 2194
    Beta strandi222 – 2298
    Beta strandi241 – 2444
    Helixi246 – 2494
    Helixi250 – 25910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AO5X-ray2.60A/B25-261[»]
    ProteinModelPortaliP36368.
    SMRiP36368. Positions 25-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP36368.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP36368.
    KOiK09462.
    PhylomeDBiP36368.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36368-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWFLILFLAL SLGGIDAAPP LQSRVVGGFN CKKNSQPWQV AVYYQKEHIC    50
    GGVLLDRNWV LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG 100
    FNMSLLMLQT IPPGADFSND LMLLRLSKPA DITDVVKPIA LPTKEPKPGS 150
    KCLASGWGSI TPTRWQKPDD LQCVFITLLP NENCAKVYLQ KVTDVMLCAG 200
    EMGGGKDTCR DDSGGPLICD GILQGTTSYG PVPCGKPGVP AIYTNLIKFN 250
    SWIKDTMMKN A 261
    Length:261
    Mass (Da):28,689
    Last modified:June 1, 1994 - v1
    Checksum:iC2854D057877F602
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti119 – 1191N → D(PubMed:3322387)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17982, M17980, M17981 Genomic DNA. Translation: AAA37680.1.
    X58628 mRNA. Translation: CAA41482.1.
    M18612 Genomic DNA. Translation: AAA39354.1.
    PIRiA41020.
    RefSeqiNP_034245.3. NM_010115.6.
    UniGeneiMm.439663.

    Genome annotation databases

    GeneIDi13647.
    KEGGimmu:13647.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17982 , M17980 , M17981 Genomic DNA. Translation: AAA37680.1 .
    X58628 mRNA. Translation: CAA41482.1 .
    M18612 Genomic DNA. Translation: AAA39354.1 .
    PIRi A41020.
    RefSeqi NP_034245.3. NM_010115.6.
    UniGenei Mm.439663.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AO5 X-ray 2.60 A/B 25-261 [» ]
    ProteinModelPortali P36368.
    SMRi P36368. Positions 25-261.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P36368.

    Proteomic databases

    PaxDbi P36368.
    PRIDEi P36368.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13647.
    KEGGi mmu:13647.

    Organism-specific databases

    CTDi 13647.
    MGIi MGI:95292. Egfbp2.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P36368.
    KOi K09462.
    PhylomeDBi P36368.

    Enzyme and pathway databases

    BRENDAi 3.4.21.119. 3474.

    Miscellaneous databases

    EvolutionaryTracei P36368.
    NextBioi 290219.
    PROi P36368.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P36368.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins."
      Drinkwater C.C., Evans B.A., Richards R.I.
      Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Salivary gland.
    2. "Mouse submandibular gland prorenin-converting enzyme is a member of glandular kallikrein family."
      Kim W.S., Nakayama K., Nakagawa T., Kawamura Y., Haraguchi K., Murakami K.
      J. Biol. Chem. 266:19283-19287(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
      Tissue: Submandibular gland.
    3. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
      Evans B.A., Drinkwater C.C., Richards R.I.
      J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 16-54, NUCLEOTIDE SEQUENCE OF 70-122.
    4. "The crystal structure of the mouse glandular kallikrein-13 (prorenin converting enzyme)."
      Timm D.E.
      Protein Sci. 6:1418-1425(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

    Entry informationi

    Entry nameiEGFB2_MOUSE
    AccessioniPrimary (citable) accession number: P36368
    Secondary accession number(s): P00754
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3