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P36368

- EGFB2_MOUSE

UniProt

P36368 - EGFB2_MOUSE

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Protein
Epidermal growth factor-binding protein type B
Gene
Egfbp2, Egfbp-2, Klk-13, Klk13
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves REN2 at a dibasic site to yield mature renin.

Catalytic activityi

Hydrolyzes mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-|- pair in the N-terminus, to yield mature renin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system
Active sitei120 – 1201Charge relay system
Active sitei213 – 2131Charge relay system

GO - Molecular functioni

  1. peptidase activity Source: MGI
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. zymogen activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.119. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor-binding protein type B (EC:3.4.21.119)
Short name:
EGF-BP B
Alternative name(s):
Glandular kallikrein K13
Short name:
mGK-13
Prorenin-converting enzyme 1
Short name:
PRECE-1
Tissue kallikrein 13
Gene namesi
Name:Egfbp2
Synonyms:Egfbp-2, Klk-13, Klk13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:95292. Egfbp2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Inferred
Add
BLAST
Propeptidei19 – 246Activation peptide
PRO_0000027983
Chaini25 – 261237Epidermal growth factor-binding protein type B
PRO_0000027984Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi102 – 1021N-linked (GlcNAc...)
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP36368.
PRIDEiP36368.

PTM databases

PhosphoSiteiP36368.

Expressioni

Gene expression databases

GenevestigatoriP36368.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi39 – 446
Beta strandi47 – 5610
Beta strandi59 – 624
Beta strandi72 – 765
Beta strandi79 – 824
Beta strandi92 – 976
Helixi103 – 1075
Beta strandi122 – 1287
Beta strandi134 – 1363
Beta strandi151 – 1577
Beta strandi172 – 1798
Helixi181 – 1877
Beta strandi196 – 2005
Beta strandi216 – 2194
Beta strandi222 – 2298
Beta strandi241 – 2444
Helixi246 – 2494
Helixi250 – 25910

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AO5X-ray2.60A/B25-261[»]
ProteinModelPortaliP36368.
SMRiP36368. Positions 25-261.

Miscellaneous databases

EvolutionaryTraceiP36368.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP36368.
KOiK09462.
PhylomeDBiP36368.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36368-1 [UniParc]FASTAAdd to Basket

« Hide

MWFLILFLAL SLGGIDAAPP LQSRVVGGFN CKKNSQPWQV AVYYQKEHIC    50
GGVLLDRNWV LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG 100
FNMSLLMLQT IPPGADFSND LMLLRLSKPA DITDVVKPIA LPTKEPKPGS 150
KCLASGWGSI TPTRWQKPDD LQCVFITLLP NENCAKVYLQ KVTDVMLCAG 200
EMGGGKDTCR DDSGGPLICD GILQGTTSYG PVPCGKPGVP AIYTNLIKFN 250
SWIKDTMMKN A 261
Length:261
Mass (Da):28,689
Last modified:June 1, 1994 - v1
Checksum:iC2854D057877F602
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191N → D1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17982, M17980, M17981 Genomic DNA. Translation: AAA37680.1.
X58628 mRNA. Translation: CAA41482.1.
M18612 Genomic DNA. Translation: AAA39354.1.
PIRiA41020.
RefSeqiNP_034245.3. NM_010115.6.
UniGeneiMm.439663.

Genome annotation databases

GeneIDi13647.
KEGGimmu:13647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17982 , M17980 , M17981 Genomic DNA. Translation: AAA37680.1 .
X58628 mRNA. Translation: CAA41482.1 .
M18612 Genomic DNA. Translation: AAA39354.1 .
PIRi A41020.
RefSeqi NP_034245.3. NM_010115.6.
UniGenei Mm.439663.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AO5 X-ray 2.60 A/B 25-261 [» ]
ProteinModelPortali P36368.
SMRi P36368. Positions 25-261.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P36368.

Proteomic databases

PaxDbi P36368.
PRIDEi P36368.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13647.
KEGGi mmu:13647.

Organism-specific databases

CTDi 13647.
MGIi MGI:95292. Egfbp2.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P36368.
KOi K09462.
PhylomeDBi P36368.

Enzyme and pathway databases

BRENDAi 3.4.21.119. 3474.

Miscellaneous databases

EvolutionaryTracei P36368.
NextBioi 290219.
PROi P36368.
SOURCEi Search...

Gene expression databases

Genevestigatori P36368.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins."
    Drinkwater C.C., Evans B.A., Richards R.I.
    Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Salivary gland.
  2. "Mouse submandibular gland prorenin-converting enzyme is a member of glandular kallikrein family."
    Kim W.S., Nakayama K., Nakagawa T., Kawamura Y., Haraguchi K., Murakami K.
    J. Biol. Chem. 266:19283-19287(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Submandibular gland.
  3. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
    Evans B.A., Drinkwater C.C., Richards R.I.
    J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-54, NUCLEOTIDE SEQUENCE OF 70-122.
  4. "The crystal structure of the mouse glandular kallikrein-13 (prorenin converting enzyme)."
    Timm D.E.
    Protein Sci. 6:1418-1425(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

Entry informationi

Entry nameiEGFB2_MOUSE
AccessioniPrimary (citable) accession number: P36368
Secondary accession number(s): P00754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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