P36368 (EGFB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epidermal growth factor-binding protein type B
Short name=EGF-BP B
EC=3.4.21.119

Alternative name(s):
Glandular kallikrein K13
Short name=mGK-13
Prorenin-converting enzyme 1
Short name=PRECE-1
Tissue kallikrein 13

Gene names

Name:	Egfbp2
Synonyms:	Egfbp-2, Klk-13, Klk13

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves REN2 at a dibasic site to yield mature renin.
Catalytic activity	Hydrolyzes mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-|- pair in the N-terminus, to yield mature renin.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW zymogen activation Inferred from direct assay Ref.2PubMed 1959648PubMed 9507064. Source: MGI
Cellular_component	extracellular space Inferred from direct assay Ref.2PubMed 1959648. Source: MGI
Molecular_function	peptidase activity Inferred from direct assay Ref.2PubMed 1959648PubMed 9507064. Source: MGI serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Probable

Propeptide

19 – 24

6

Activation peptide

PRO_0000027983

Chain

25 – 261

237

Epidermal growth factor-binding protein type B

PRO_0000027984

Regions

Domain

25 – 258

234

Peptidase S1

Sites

Active site

65

1

Charge relay system

Active site

120

1

Charge relay system

Active site

213

1

Charge relay system

Amino acid modifications

Glycosylation

102

1

N-linked (GlcNAc...)

Disulfide bond

31 ↔ 173

Disulfide bond

50 ↔ 66

Disulfide bond

152 ↔ 219

Disulfide bond

184 ↔ 198

Disulfide bond

209 ↔ 234

Experimental info

Sequence conflict

119

1

N → D Ref.1

Secondary structure

1

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261

Helix Strand Turn

Details...

Beta strand

39 – 44

6

Beta strand

47 – 56

10

Beta strand

59 – 62

4

Beta strand

72 – 76

5

Beta strand

79 – 82

4

Beta strand

92 – 97

6

Helix

103 – 107

5

Beta strand

122 – 128

7

Beta strand

134 – 136

3

Beta strand

151 – 157

7

Beta strand

172 – 179

8

Helix

181 – 187

7

Beta strand

196 – 200

5

Beta strand

216 – 219

4

Beta strand

222 – 229

8

Beta strand

241 – 244

4

Helix

246 – 249

4

Helix

250 – 259

10

Sequences

	Sequence		Length	Mass (Da)
	P36368 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: C2854D057877F602	FASTA	261	28,689
	10 20 30 40 50 60 MWFLILFLAL SLGGIDAAPP LQSRVVGGFN CKKNSQPWQV AVYYQKEHIC GGVLLDRNWV 70 80 90 100 110 120 LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG FNMSLLMLQT IPPGADFSND 130 140 150 160 170 180 LMLLRLSKPA DITDVVKPIA LPTKEPKPGS KCLASGWGSI TPTRWQKPDD LQCVFITLLP 190 200 210 220 230 240 NENCAKVYLQ KVTDVMLCAG EMGGGKDTCR DDSGGPLICD GILQGTTSYG PVPCGKPGVP 250 260 AIYTNLIKFN SWIKDTMMKN A

References

[1]	"Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins." Drinkwater C.C., Evans B.A., Richards R.I. Biochemistry 26:6750-6756(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Salivary gland.
[2]	"Mouse submandibular gland prorenin-converting enzyme is a member of glandular kallikrein family." Kim W.S., Nakayama K., Nakagawa T., Kawamura Y., Haraguchi K., Murakami K. J. Biol. Chem. 266:19283-19287(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Submandibular gland.
[3]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-54, NUCLEOTIDE SEQUENCE OF 70-122.
[4]	"The crystal structure of the mouse glandular kallikrein-13 (prorenin converting enzyme)." Timm D.E. Protein Sci. 6:1418-1425(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17982, M17980, M17981 Genomic DNA. Translation: AAA37680.1.
X58628 mRNA. Translation: CAA41482.1.
M18612 Genomic DNA. Translation: AAA39354.1.

IPI

IPI00121818.

PIR

A41020.

RefSeq

NP_034245.3. NM_010115.6.

UniGene

Mm.439663.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AO5	X-ray	2.60	A/B	25-261	[»]

ProteinModelPortal

P36368.

SMR

P36368. Positions 25-261.

ModBase

Search...

PTM databases

PhosphoSite

P36368.

Proteomic databases

PaxDb

P36368.

PRIDE

P36368.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

13647.

KEGG

mmu:13647.

Organism-specific databases

CTD

13647.

MGI

MGI:95292. Egfbp2.

Phylogenomic databases

eggNOG

COG5640.

HOGENOM

HOG000251820.

HOVERGEN

HBG013304.

InParanoid

P36368.

KO

K09462.

OrthoDB

EOG4DZ1VW.

Enzyme and pathway databases

BRENDA

3.4.21.119. 3474.

Gene expression databases

Genevestigator

P36368.

GermOnline

ENSMUSG00000053719. Mus musculus.

Family and domain databases

InterPro

IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P36368.

NextBio

290219.

SOURCE

Search...

Entry information

Entry name

EGFB2_MOUSE

Accession

Primary (citable) accession number: P36368
Secondary accession number(s): P00754

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program