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Protein

Epidermal growth factor-binding protein type B

Gene

Egfbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves REN2 at a dibasic site to yield mature renin.

Catalytic activityi

Hydrolyzes mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-|- pair in the N-terminus, to yield mature renin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay system1
Active sitei120Charge relay system1
Active sitei213Charge relay system1

GO - Molecular functioni

  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • zymogen activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.119. 3474.

Protein family/group databases

MEROPSiS01.173.

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor-binding protein type B (EC:3.4.21.119)
Short name:
EGF-BP B
Alternative name(s):
Glandular kallikrein K13
Short name:
mGK-13
Prorenin-converting enzyme 1
Short name:
PRECE-1
Tissue kallikrein 13
Gene namesi
Name:Egfbp2
Synonyms:Egfbp-2, Klk-13, Klk13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95292. Egfbp2.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
PropeptideiPRO_000002798319 – 24Activation peptide6
ChainiPRO_000002798425 – 261Epidermal growth factor-binding protein type BAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi102N-linked (GlcNAc...)1
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP36368.
PaxDbiP36368.
PRIDEiP36368.

PTM databases

iPTMnetiP36368.
PhosphoSitePlusiP36368.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047488.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 44Combined sources6
Beta strandi47 – 56Combined sources10
Beta strandi59 – 62Combined sources4
Beta strandi72 – 76Combined sources5
Beta strandi79 – 82Combined sources4
Beta strandi92 – 97Combined sources6
Helixi103 – 107Combined sources5
Beta strandi122 – 128Combined sources7
Beta strandi134 – 136Combined sources3
Beta strandi151 – 157Combined sources7
Beta strandi172 – 179Combined sources8
Helixi181 – 187Combined sources7
Beta strandi196 – 200Combined sources5
Beta strandi216 – 219Combined sources4
Beta strandi222 – 229Combined sources8
Beta strandi241 – 244Combined sources4
Helixi246 – 249Combined sources4
Helixi250 – 259Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AO5X-ray2.60A/B25-261[»]
ProteinModelPortaliP36368.
SMRiP36368.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36368.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP36368.
KOiK09462.
PhylomeDBiP36368.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLILFLAL SLGGIDAAPP LQSRVVGGFN CKKNSQPWQV AVYYQKEHIC
60 70 80 90 100
GGVLLDRNWV LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG
110 120 130 140 150
FNMSLLMLQT IPPGADFSND LMLLRLSKPA DITDVVKPIA LPTKEPKPGS
160 170 180 190 200
KCLASGWGSI TPTRWQKPDD LQCVFITLLP NENCAKVYLQ KVTDVMLCAG
210 220 230 240 250
EMGGGKDTCR DDSGGPLICD GILQGTTSYG PVPCGKPGVP AIYTNLIKFN
260
SWIKDTMMKN A
Length:261
Mass (Da):28,689
Last modified:June 1, 1994 - v1
Checksum:iC2854D057877F602
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119N → D (PubMed:3322387).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17982, M17980, M17981 Genomic DNA. Translation: AAA37680.1.
X58628 mRNA. Translation: CAA41482.1.
M18612 Genomic DNA. Translation: AAA39354.1.
PIRiA41020.
RefSeqiNP_034245.3. NM_010115.6.
UniGeneiMm.439663.

Genome annotation databases

GeneIDi13647.
KEGGimmu:13647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17982, M17980, M17981 Genomic DNA. Translation: AAA37680.1.
X58628 mRNA. Translation: CAA41482.1.
M18612 Genomic DNA. Translation: AAA39354.1.
PIRiA41020.
RefSeqiNP_034245.3. NM_010115.6.
UniGeneiMm.439663.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AO5X-ray2.60A/B25-261[»]
ProteinModelPortaliP36368.
SMRiP36368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047488.

Protein family/group databases

MEROPSiS01.173.

PTM databases

iPTMnetiP36368.
PhosphoSitePlusiP36368.

Proteomic databases

MaxQBiP36368.
PaxDbiP36368.
PRIDEiP36368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13647.
KEGGimmu:13647.

Organism-specific databases

CTDi13647.
MGIiMGI:95292. Egfbp2.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP36368.
KOiK09462.
PhylomeDBiP36368.

Enzyme and pathway databases

BRENDAi3.4.21.119. 3474.

Miscellaneous databases

EvolutionaryTraceiP36368.
PROiP36368.
SOURCEiSearch...

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGFB2_MOUSE
AccessioniPrimary (citable) accession number: P36368
Secondary accession number(s): P00754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.