Reviewed, UniProtKB/Swiss-Prot P36367 (FMO4_RABIT)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 4
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 4
      Short name=FMO 4
    FMO 1E1
    Dimethylaniline oxidase 4

Gene names

Name:

FMO4

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD.
Subcellular location	Microsome membrane. Endoplasmic reticulum membrane.
Tissue specificity	Kidney and liver.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 555	554	Dimethylaniline monooxygenase [N-oxide-forming] 4		PRO_0000147662
Regions
	Transmembrane	518 – 532	15	Potential
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36367-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5A7219CAD1DEB3A2	FASTA	555	62,992
	10 20 30 40 50 60 MAKKVAVIGA GVSGLTSIKC CLDEDLEPTC FERSNDIGGL WKYTETSKDG MTRIYWSLVT 70 80 90 100 110 120 NVCKEMSCYS DFPFQEDYPN FMSHSKFWNY LQEFAEHFDL LKYIQFKTTV CSVTKRPDFS 130 140 150 160 170 180 KTGQWDVVTE TEGKQHRAVF DAVMVCTGKF LNPRLPLESF PGILKFRGQI LHCQEYKIPE 190 200 210 220 230 240 GFRGQRVLVI GLGNSGGDVA VELSRVAAQV LLSTRTGTWV ISRSSNGGYP FNMMITRRCL 250 260 270 280 290 300 NVIEQVLPSC FLRWINERQM NKRFNHENYG LSITKGKNPK FIVNDELPTC ILCGTVTVKT 310 320 330 340 350 360 SVKEFTETSA IFEDGTVEEN IDSVIFTTGY VFSFPFLEEP LRSLCMKKMF LYKHVFPSNL 370 380 390 400 410 420 ERASMAIIGL ISLKGSILTG TELQARWATR VFKGLCKIPP PQQLMAEVTK KEELIKRGVI 430 440 450 460 470 480 KDTSEEKLSY IPYMDDLAAC IGTKPNIPLL FLKDPRLAWE VFFGPCTPYQ YRLMGPGKWD 490 500 510 520 530 540 GARNAILTQW DRTLKPLKTR TVSSDSSKSA SLSHYLKVWG APLLLASVLL ICKSSHFLKS 550 VRDKLQNRIF PYLVL

References

[1]

"Cloning and sequencing of flavin-containing monooxygenases FMO3 and FMO4 from rabbit and characterization of FMO3." Burnett V.L., Lawton M.P., Philpot R.M. J. Biol. Chem. 269:14314-14322(1994) [PubMed: 8188717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10392 mRNA. Translation: AAA21177.1.
PIR	A54096.
RefSeq	NP_001076253.1.
UniGene	Ocu.1822
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSOCUG00000002795. Oryctolagus cuniculus. [Contig view]
GeneID	100009582.
Phylogenomic databases
HOVERGEN	P36367.
Enzyme and pathway databases
BRENDA	1.14.13.8. 255.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR002256. Flavin_mOase_4. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01124. FMOXYGENASE4.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

FMO4_RABIT

Accession

Primary (citable) accession number: P36367

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)