Reviewed, UniProtKB/Swiss-Prot P36366 (FMO2_CAVPO)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 2
    EC=1.14.13.8
Alternative name(s):
    Pulmonary flavin-containing monooxygenase 2
      Short name=FMO 2
    FMO 1B1
    Dimethylaniline oxidase 2

Gene names

Name:	FMO2
Synonyms:	FMO-2

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD By similarity. Magnesium By similarity.
Subcellular location	Microsome membrane. Endoplasmic reticulum membrane.
Tissue specificity	Lung.
Polymorphism	There are two allelic forms (A and B).
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Ligand	FAD Flavoprotein Magnesium NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 535	534	Dimethylaniline monooxygenase [N-oxide-forming] 2		PRO_0000147644
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Natural variations
	Natural variant	522	1	V → L in B form.
	Natural variant	530	1	S → F in B form.

Sequences

	Sequence		Length	Mass (Da)
	P36366-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 10D9DDA06EB58DA2	FASTA	535	60,942
	10 20 30 40 50 60 MAKKVAVIGA GVSGLISLKC CVDEGLEPTC FERTEDIGGL WRFKENVEDG RASIYKSVIT 70 80 90 100 110 120 NTSKEMSCFS DFPMPEDFPN FLHNSKLLEY FRLFAKKFDL LKYIQFQTTV LTVKKHPDFS 130 140 150 160 170 180 SSGQWEVVTQ SDGKEQSAVF DAVMVCSGHH ILPHIPLKSF PGIERFKGQY FHSRQYKHPA 190 200 210 220 230 240 GFEGKRILVI GIGNSASDIA SELSKNAAQV FISTRNGSWV MSRISEDGYP WDMVFHTRFK 250 260 270 280 290 300 SMLRNILPRT VSKWMMEQQL NRWFNHANYS LEPKNKYLMK EPILNDDLPS RILYGAVKVK 310 320 330 340 350 360 SRVTQLTETS ALFEDGTVEE DIDVIVFATG YTFSFPFLEE SLVKIEHNMV SLYKYMFPPQ 370 380 390 400 410 420 LEKPTLTCMG LIQPLGSIFP TVELQARWAT RVFKGLCHLP SEKTMMEDII KRNEKRIDLF 430 440 450 460 470 480 GESQSQIVQT NYVDYLDELA LEIGAKPDLI SFLLKDPELA VKLCFGPCNS YQYRLVGPGQ 490 500 510 520 530 WEGARRAILT QKQRILKPLK TRSVKAAPNL SASFLMKILA LVAVFVAFFS QLYGF

References

[1]

"Guinea pig or rabbit lung flavin-containing monooxygenases with distinct mobilities in SDS-PAGE are allelic variants that differ at only two positions." Nikbakht K.N., Lawton M.P., Philpot R.M. Pharmacogenetics 2:207-216(1992) [PubMed: 1306120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hartley. Tissue: Lung.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10037 Genomic DNA. Translation: AAB59631.1.
3D structure databases
SMR	P36366. Positions 3-440.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000014127; ENSCPOP00000012602; ENSCPOG00000013986; Cavia porcellus. [Genome view]
Phylogenomic databases
eggNOG	roNOG08104.
HOVERGEN	P36366.
InParanoid	P36366.
Enzyme and pathway databases
BRENDA	1.14.13.8. 44.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR020946. Flavin_mOase-like. IPR002254. Flavin_mOase_2. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01122. FMOXYGENASE2.
ProtoNet	Search...

Entry information

Entry name

FMO2_CAVPO

Accession

Primary (citable) accession number: P36366
Secondary accession number(s): Q05194

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)