Reviewed, UniProtKB/Swiss-Prot P36365 (FMO1_RAT)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 1
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 1
      Short name=FMO 1
    Dimethylaniline oxidase 1

Gene names

Name:	Fmo1
Synonyms:	Fmo-1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD.
Subcellular location	Microsome membrane. Endoplasmic reticulum membrane.
Tissue specificity	Liver.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Dimethylaniline monooxygenase [N-oxide-forming] 1		PRO_0000147643
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Sites
	Site	208	1	Important for substrate binding By similarity
Experimental info
	Sequence conflict	141	1	D → A in AAA41165. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36365-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 188EABAF96653977	FASTA	532	59,825
	10 20 30 40 50 60 MVKRVAIVGA GVSGLASIKC CLEEGLEPTC FERSCDLGGL WRFTEHVEEG RASLYNSVVS 70 80 90 100 110 120 NSSKEMSCYS DFPFPEDYPN FVPNSLFLEY LQLYATQFNL LRCIYFNTKV CSITKRPDFA 130 140 150 160 170 180 VSGQWEVVTV CQGKQSSDTF DAVMVCTGFL TNPHLPLDSF PGIQTFKGQY FHSRQYKHPD 190 200 210 220 230 240 VFKDKRVLVV GMGNSGTDIA VEASHLAKKV FLSTTGGAWV ISRVFDSGYP WDMIFMTRFQ 250 260 270 280 290 300 NMLRNLLPTP VVSWLISKKM NSWFNHVNYG VAPEDRTQLR EPVLNDELPG RIITGKVLIK 310 320 330 340 350 360 PSIKEVKENS VVFNNTPKEE PIDVIVFATG YSFAFPFLDE SIVKVEDGQA SLYKYIFPAH 370 380 390 400 410 420 LPKPTLAVIG LIKPLGSMIP TGETQARWVV QVLKGATTLP PPSVMMKEVN ERKKNKHSGF 430 440 450 460 470 480 GLCYCKALQS DYITYIDDLL TSINAKPDLR AMLLTDPRLA LSIFFGPCTP YHFRLTGPGK 490 500 510 520 530 WEGARKAILT QWDRTVNVTK TRTVQETPST FETLLKLFSF LALLVAVFFI FL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat liver flavin-containing monooxygenase (FMO): cDNA cloning and expression in yeast." Itoh K., Kimura T., Yokoi T., Itoh S., Kamataki T. Biochim. Biophys. Acta 1173:165-171(1993) [PubMed: 8504165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	"Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells." Hay J.C., Chao D.S., Kuo C.S., Scheller R.H. Cell 89:149-158(1997) [PubMed: 9094723] [Abstract] Cited for: PROTEIN SEQUENCE OF 34-42 AND 52-56. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84719 mRNA. Translation: AAA41165.1. BC061567 mRNA. Translation: AAH61567.1.
IPI	IPI00201564.
PIR	S33758.
RefSeq	NP_036924.1.
UniGene	Rn.867
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P36365.
Genome annotation databases
Ensembl	ENSRNOG00000034191. Rattus norvegicus. [Contig view]
GeneID	25256.
KEGG	rno:25256.
Organism-specific databases
RGD	2622. Fmo1.
Phylogenomic databases
HOVERGEN	P36365.
OMA	P36365. ITGKVFI.
Enzyme and pathway databases
BRENDA	1.14.13.8. 248.
Gene expression databases
ArrayExpress	P36365.
GermOnline	ENSRNOG00000034191. Rattus norvegicus.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR002253. Flavin_mOase_1. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01121. FMOXYGENASE1.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	605891.

Entry information

Entry name

FMO1_RAT

Accession

Primary (citable) accession number: P36365
Secondary accession number(s): Q6P7Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)