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P36362 (CHIT_MANSE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Digests chitin in the exoskeleton during the molting process.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Subcellular location

Secreted.

Tissue specificity

Epidermis and gut.

Developmental stage

High levels seen in the epidermis on day 0, but rapidly disappears and is undetected on days 1-4 of fifth instar. It reappears on day 5 and peaks on day 7 after which a rapid decline is seen. In the gut is detected on day 6 with lower levels seen on days 0, 7 and 8.

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Contains 1 chitin-binding type-2 domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Chitin degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   LigandChitin-binding
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processchitin catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

polysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionchitin binding

Inferred from electronic annotation. Source: UniProtKB-KW

chitinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 554535Endochitinase
PRO_0000011949

Regions

Domain495 – 55359Chitin-binding type-2
Compositional bias396 – 45358Ser/Thr-rich

Sites

Active site1461Proton donor By similarity

Amino acid modifications

Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation4071N-linked (GlcNAc...) Potential
Glycosylation5451N-linked (GlcNAc...) Potential
Disulfide bond527 ↔ 540 By similarity

Sequences

Sequence LengthMass (Da)Tools
P36362 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 3989D756C96CD490

FASTA55462,204
        10         20         30         40         50         60 
MRATLATLAV LALATAVQSD SRARIVCYFS NWAVYRPGVG RYGIEDIPVE KCTHIIYSFI 

        70         80         90        100        110        120 
GVTEGNSEVL IIDPELDVDK NGFRNFTSLR SSHPSVKFMV AVGGWAEGSS KYSHMVAQKS 

       130        140        150        160        170        180 
TRMSFIRSVV SFLKKYDFDG LDLDWEYPGA ADRGGSFSDK DKFLYLVQEL RRAFIRVGKG 

       190        200        210        220        230        240 
WELTAAVPLA NFRLMEGYHV PELCQELDAI HVMSYDLRGN WAGFADVHSP LYKRPHDQWA 

       250        260        270        280        290        300 
YEKLNVNDGL HLWEEKGCPS NKLVVGIPFY GRSFTLSAGN NNYGLGTFIN KEAGGGDPAP 

       310        320        330        340        350        360 
YTNATGFWAY YEICTEVDKD DSGWTKKWDE QGKCPYAYKG TQWVGYEDPR SVEIKMNWIK 

       370        380        390        400        410        420 
QKGYLGAMTW AIDMDDFQGL CGEKNPLIKI LHKHMSSYTV PPPHTENTTP TPEWARPPST 

       430        440        450        460        470        480 
PSDPSEGDPI PTTTTAKPAS TTKTTVKTTT TTTAKPPQSV IDEENDINVR PEPKPEPQPE 

       490        500        510        520        530        540 
PEVEVPPTEN EVDGSEICNS DQDYIPDKKH CDKYWRCVNG EAMQFSCQHG TVFNVELNVC 

       550 
DWPSNATRRE CQQP 

« Hide

References

[1]"Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta."
Kramer K.J., Corpuz L., Choi H.K., Muthukrishnan S.
Insect Biochem. Mol. Biol. 23:691-701(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase."
Choi H.K., Choi K.H., Kramer K.J., Muthukrishnan S.
Insect Biochem. Mol. Biol. 27:37-47(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02270 mRNA. Translation: AAC04924.1.
L49234 Genomic DNA. Translation: AAB53952.1.
PIRA56596.

3D structure databases

ProteinModelPortalP36362.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.170.140.10. 1 hit.
3.20.20.80. 2 hits.
InterProIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF57625. SSF57625. 1 hit.
PROSITEPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHIT_MANSE
AccessionPrimary (citable) accession number: P36362
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 16, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries