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Protein

Endochitinase

Gene
N/A
Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Digests chitin in the exoskeleton during the molting process.

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei146Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM14 Carbohydrate-Binding Module Family 14
GH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase (EC:3.2.1.14)
OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifieri7130 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001194920 – 554EndochitinaseAdd BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi527 ↔ 540PROSITE-ProRule annotation
Glycosylationi545N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP36362

Expressioni

Tissue specificityi

Epidermis and gut.

Developmental stagei

High levels seen in the epidermis on day 0, but rapidly disappears and is undetected on days 1-4 of fifth instar. It reappears on day 5 and peaks on day 7 after which a rapid decline is seen. In the gut is detected on day 6 with lower levels seen on days 0, 7 and 8.

Structurei

3D structure databases

ProteinModelPortaliP36362
SMRiP36362
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini495 – 553Chitin-binding type-2PROSITE-ProRule annotationAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi396 – 453Ser/Thr-richAdd BLAST58

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.50.10, 1 hit
InterProiView protein in InterPro
IPR002557 Chitin-bd_dom
IPR036508 Chitin-bd_dom_sf
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF01607 CBM_14, 1 hit
PF00704 Glyco_hydro_18, 1 hit
SMARTiView protein in SMART
SM00494 ChtBD2, 1 hit
SM00636 Glyco_18, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit
SSF57625 SSF57625, 1 hit
PROSITEiView protein in PROSITE
PS50940 CHIT_BIND_II, 1 hit
PS01095 CHITINASE_18, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRATLATLAV LALATAVQSD SRARIVCYFS NWAVYRPGVG RYGIEDIPVE
60 70 80 90 100
KCTHIIYSFI GVTEGNSEVL IIDPELDVDK NGFRNFTSLR SSHPSVKFMV
110 120 130 140 150
AVGGWAEGSS KYSHMVAQKS TRMSFIRSVV SFLKKYDFDG LDLDWEYPGA
160 170 180 190 200
ADRGGSFSDK DKFLYLVQEL RRAFIRVGKG WELTAAVPLA NFRLMEGYHV
210 220 230 240 250
PELCQELDAI HVMSYDLRGN WAGFADVHSP LYKRPHDQWA YEKLNVNDGL
260 270 280 290 300
HLWEEKGCPS NKLVVGIPFY GRSFTLSAGN NNYGLGTFIN KEAGGGDPAP
310 320 330 340 350
YTNATGFWAY YEICTEVDKD DSGWTKKWDE QGKCPYAYKG TQWVGYEDPR
360 370 380 390 400
SVEIKMNWIK QKGYLGAMTW AIDMDDFQGL CGEKNPLIKI LHKHMSSYTV
410 420 430 440 450
PPPHTENTTP TPEWARPPST PSDPSEGDPI PTTTTAKPAS TTKTTVKTTT
460 470 480 490 500
TTTAKPPQSV IDEENDINVR PEPKPEPQPE PEVEVPPTEN EVDGSEICNS
510 520 530 540 550
DQDYIPDKKH CDKYWRCVNG EAMQFSCQHG TVFNVELNVC DWPSNATRRE

CQQP
Length:554
Mass (Da):62,204
Last modified:June 1, 1994 - v1
Checksum:i3989D756C96CD490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02270 mRNA Translation: AAC04924.1
L49234 Genomic DNA Translation: AAB53952.1
PIRiA56596

Similar proteinsi

Entry informationi

Entry nameiCHIT_MANSE
AccessioniPrimary (citable) accession number: P36362
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

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