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P36362

- CHIT_MANSE

UniProt

P36362 - CHIT_MANSE

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Protein

Endochitinase

Gene
N/A
Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Digests chitin in the exoskeleton during the molting process.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461Proton donor By similarity

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC
  2. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-KW
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase (EC:3.2.1.14)
OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Taxonomic identifieri7130 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed predictionAdd
BLAST
Chaini20 – 554535EndochitinasePRO_0000011949Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi303 – 3031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi407 – 4071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi527 ↔ 540 By similarity
Glycosylationi545 – 5451N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Epidermis and gut.

Developmental stagei

High levels seen in the epidermis on day 0, but rapidly disappears and is undetected on days 1-4 of fifth instar. It reappears on day 5 and peaks on day 7 after which a rapid decline is seen. In the gut is detected on day 6 with lower levels seen on days 0, 7 and 8.

Structurei

3D structure databases

ProteinModelPortaliP36362.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini495 – 55359Chitin-binding type-2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi396 – 45358Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEiPS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36362-1 [UniParc]FASTAAdd to Basket

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MRATLATLAV LALATAVQSD SRARIVCYFS NWAVYRPGVG RYGIEDIPVE    50
KCTHIIYSFI GVTEGNSEVL IIDPELDVDK NGFRNFTSLR SSHPSVKFMV 100
AVGGWAEGSS KYSHMVAQKS TRMSFIRSVV SFLKKYDFDG LDLDWEYPGA 150
ADRGGSFSDK DKFLYLVQEL RRAFIRVGKG WELTAAVPLA NFRLMEGYHV 200
PELCQELDAI HVMSYDLRGN WAGFADVHSP LYKRPHDQWA YEKLNVNDGL 250
HLWEEKGCPS NKLVVGIPFY GRSFTLSAGN NNYGLGTFIN KEAGGGDPAP 300
YTNATGFWAY YEICTEVDKD DSGWTKKWDE QGKCPYAYKG TQWVGYEDPR 350
SVEIKMNWIK QKGYLGAMTW AIDMDDFQGL CGEKNPLIKI LHKHMSSYTV 400
PPPHTENTTP TPEWARPPST PSDPSEGDPI PTTTTAKPAS TTKTTVKTTT 450
TTTAKPPQSV IDEENDINVR PEPKPEPQPE PEVEVPPTEN EVDGSEICNS 500
DQDYIPDKKH CDKYWRCVNG EAMQFSCQHG TVFNVELNVC DWPSNATRRE 550
CQQP 554
Length:554
Mass (Da):62,204
Last modified:June 1, 1994 - v1
Checksum:i3989D756C96CD490
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02270 mRNA. Translation: AAC04924.1.
L49234 Genomic DNA. Translation: AAB53952.1.
PIRiA56596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02270 mRNA. Translation: AAC04924.1 .
L49234 Genomic DNA. Translation: AAB53952.1 .
PIRi A56596.

3D structure databases

ProteinModelPortali P36362.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM14. Carbohydrate-Binding Module Family 14.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.140.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR002557. Chitin-bd_dom.
IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01607. CBM_14. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view ]
SMARTi SM00494. ChtBD2. 1 hit.
SM00636. Glyco_18. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF57625. SSF57625. 1 hit.
PROSITEi PS50940. CHIT_BIND_II. 1 hit.
PS01095. CHITINASE_18. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta."
    Kramer K.J., Corpuz L., Choi H.K., Muthukrishnan S.
    Insect Biochem. Mol. Biol. 23:691-701(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase."
    Choi H.K., Choi K.H., Kramer K.J., Muthukrishnan S.
    Insect Biochem. Mol. Biol. 27:37-47(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiCHIT_MANSE
AccessioniPrimary (citable) accession number: P36362
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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