Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P36362

- CHIT_MANSE

UniProt

P36362 - CHIT_MANSE

Protein

Endochitinase

Gene
N/A
Organism
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Digests chitin in the exoskeleton during the molting process.

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB-EC
    2. chitin binding Source: UniProtKB-KW

    GO - Biological processi

    1. chitin catabolic process Source: UniProtKB-KW
    2. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

    Keywords - Ligandi

    Chitin-binding

    Protein family/group databases

    CAZyiCBM14. Carbohydrate-Binding Module Family 14.
    GH18. Glycoside Hydrolase Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endochitinase (EC:3.2.1.14)
    OrganismiManduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
    Taxonomic identifieri7130 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaSphingidaeSphinginaeSphinginiManduca

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 554535EndochitinasePRO_0000011949Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi527 ↔ 540PROSITE-ProRule annotation
    Glycosylationi545 – 5451N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Epidermis and gut.

    Developmental stagei

    High levels seen in the epidermis on day 0, but rapidly disappears and is undetected on days 1-4 of fifth instar. It reappears on day 5 and peaks on day 7 after which a rapid decline is seen. In the gut is detected on day 6 with lower levels seen on days 0, 7 and 8.

    Structurei

    3D structure databases

    ProteinModelPortaliP36362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini495 – 55359Chitin-binding type-2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi396 – 45358Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 chitin-binding type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.170.140.10. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR002557. Chitin-bd_dom.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01607. CBM_14. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view]
    SMARTiSM00494. ChtBD2. 1 hit.
    SM00636. Glyco_18. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    SSF57625. SSF57625. 1 hit.
    PROSITEiPS50940. CHIT_BIND_II. 1 hit.
    PS01095. CHITINASE_18. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36362-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRATLATLAV LALATAVQSD SRARIVCYFS NWAVYRPGVG RYGIEDIPVE    50
    KCTHIIYSFI GVTEGNSEVL IIDPELDVDK NGFRNFTSLR SSHPSVKFMV 100
    AVGGWAEGSS KYSHMVAQKS TRMSFIRSVV SFLKKYDFDG LDLDWEYPGA 150
    ADRGGSFSDK DKFLYLVQEL RRAFIRVGKG WELTAAVPLA NFRLMEGYHV 200
    PELCQELDAI HVMSYDLRGN WAGFADVHSP LYKRPHDQWA YEKLNVNDGL 250
    HLWEEKGCPS NKLVVGIPFY GRSFTLSAGN NNYGLGTFIN KEAGGGDPAP 300
    YTNATGFWAY YEICTEVDKD DSGWTKKWDE QGKCPYAYKG TQWVGYEDPR 350
    SVEIKMNWIK QKGYLGAMTW AIDMDDFQGL CGEKNPLIKI LHKHMSSYTV 400
    PPPHTENTTP TPEWARPPST PSDPSEGDPI PTTTTAKPAS TTKTTVKTTT 450
    TTTAKPPQSV IDEENDINVR PEPKPEPQPE PEVEVPPTEN EVDGSEICNS 500
    DQDYIPDKKH CDKYWRCVNG EAMQFSCQHG TVFNVELNVC DWPSNATRRE 550
    CQQP 554
    Length:554
    Mass (Da):62,204
    Last modified:June 1, 1994 - v1
    Checksum:i3989D756C96CD490
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02270 mRNA. Translation: AAC04924.1.
    L49234 Genomic DNA. Translation: AAB53952.1.
    PIRiA56596.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02270 mRNA. Translation: AAC04924.1 .
    L49234 Genomic DNA. Translation: AAB53952.1 .
    PIRi A56596.

    3D structure databases

    ProteinModelPortali P36362.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM14. Carbohydrate-Binding Module Family 14.
    GH18. Glycoside Hydrolase Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.140.10. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR002557. Chitin-bd_dom.
    IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01607. CBM_14. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view ]
    SMARTi SM00494. ChtBD2. 1 hit.
    SM00636. Glyco_18. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    SSF57625. SSF57625. 1 hit.
    PROSITEi PS50940. CHIT_BIND_II. 1 hit.
    PS01095. CHITINASE_18. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta."
      Kramer K.J., Corpuz L., Choi H.K., Muthukrishnan S.
      Insect Biochem. Mol. Biol. 23:691-701(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase."
      Choi H.K., Choi K.H., Kramer K.J., Muthukrishnan S.
      Insect Biochem. Mol. Biol. 27:37-47(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiCHIT_MANSE
    AccessioniPrimary (citable) accession number: P36362
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3