P36361 (CHI5_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endochitinase CH5B EC=3.2.1.14
Organism	Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier	3885 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Vacuole By similarity. Note: Vacuolar and protoplast By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Vacuole
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Chain	27 – 316	290	Endochitinase CH5B		PRO_0000005313
	Propeptide	317 – 327	11	Removed in mature form Probable		PRO_0000005314
Regions
	Domain	27 – 67	41	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	29 ↔ 44		By similarity
	Disulfide bond	38 ↔ 50		By similarity
	Disulfide bond	43 ↔ 57		By similarity
	Disulfide bond	61 ↔ 65		By similarity
	Disulfide bond	96 ↔ 158		By similarity
	Disulfide bond	169 ↔ 177		By similarity
	Disulfide bond	276 ↔ 308		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36361 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 5F13239464A9C7D8	FASTA	327	35,291
	10 20 30 40 50 60 MKKNRMMIMI CSVGVVWMLL VGGSYGEQCG RQAGGALCPG GNCCSQFGWC GSTTDYCGKD 70 80 90 100 110 120 CQSQCGGPSP APTDLSALIS RSTFDQVLKH RNDGACPAKG FYTYDAFIAA AKAYPSFGNT 130 140 150 160 170 180 GDTATRKREI AAFLGQTSHE TTGGWATAPD GPYAWGYCFV RERNPSAYCS ATPQFPCAPG 190 200 210 220 230 240 QQYYGRGPIQ ISWNYNYGQC GRAIGVDLLN KPDLVATDSV ISFKSALWFW MTAQSPKPSS 250 260 270 280 290 300 HDVITSRWTP SSADVAARRL PGYGTVTNII NGGLECGRGQ DSRVQDRIGF FKRYCDLLGV 310 320 GYGNNLDCYS QTPFGNSLFL SDLVTSQ

References

[1]	"Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco." Broglie K.E., Biddle P., Cressman R., Broglie R. Plant Cell 1:599-607(1989) [PubMed: 2535512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Saxa.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S43926 Genomic DNA. Translation: AAB23263.1.
PIR	JQ0965.
3D structure databases
ProteinModelPortal	P36361.
SMR	P36361. Positions 27-315.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI5_PHAVU

Accession

Primary (citable) accession number: P36361

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 31, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program