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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (strain RVA/Turkey/Ireland/Ty-1/1978/G7P[17]) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).By similarity

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

GO - Biological processi

Keywordsi

Biological processHost-virus interaction
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (strain RVA/Turkey/Ireland/Ty-1/1978/G7P[17]) (RV-A)
Taxonomic identifieri12584 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiAves [TaxID: 8782]

Subcellular locationi

  • Virion By similarity
  • Host endoplasmic reticulum lumen Curated

  • Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 53Sequence analysisAdd BLAST53
ChainiPRO_000014958954 – 329Outer capsid glycoprotein VP7Sequence analysisAdd BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi150N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi321N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid.By similarity
N-glycosylated.By similarity
Intramolecular disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 (By similarity).By similarity

Structurei

3D structure databases

SMRiP36357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR001963. VP7.
PfamiView protein in Pfam
PF00434. VP7. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000191. VP7. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSTECTILL IEIIFYFLAA IILYDMLHKM ANSPLLCIAV LTVTLAVTSK
60 70 80 90 100
CYAQNYGINV PITGSMDVAV PNKTDDQIGL SSTLCIYYPK EAATQMNDAE
110 120 130 140 150
WKSTVTQLLL AKGWPTTSVY LNEYADLQSF SNDPQLNCDY NIILAKYDQN
160 170 180 190 200
ETLDMSELAE LLLYEWLCNP MDVTLYYYQQ TSESNKWIAM GSDCTIKVCP
210 220 230 240 250
LNTQTLGIGC KTTDVSTFEE LTTTEKLAII DVVDGVNHKA NYTISTCTIK
260 270 280 290 300
NCIRLDPREN VAIIQVGGPE IIDISEDPMV VPHVQRATRI NWKKWWQIFY
310 320
TVVDYINTII QAMSKRSRSL NTSAYYFRV
Length:329
Mass (Da):37,288
Last modified:June 1, 1994 - v1
Checksum:iD396321A9FAE7939
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S58166 mRNA. Translation: AAB26094.1.
PIRiA48351.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVP7_ROTA1
AccessioniPrimary (citable) accession number: P36357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 10, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families