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P36313 (ICP34_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurovirulence factor ICP34.5
Alternative name(s):
Infected cell protein 34.5
protein gamma(1)34.5
Gene names
Name:RL1
Synonyms:ICP34.5
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to HSV resistance to the antiviral effects of alpha/beta interferon. Recruits the serine/threonine-protein phosphatase PPP1CA/PP1-alpha to dephosphorylate the translation initiation factor eIF-2A, thereby couteracting the host shutoff of protein synthesis involving double-stranded RNA-dependent protein kinase EIF2AK2/PKR. Also down-modulates the host MHC class II proteins cell surface expression. Acts as a neurovirulence factor that has a profound effect on the growth of the virus in central nervous system tissue, probably through its ability to maintain an environment favorable for viral replication By similarity. Ref.4

Subunit structure

Interacts with human PPP1CA to form a high-molecular-weight complex that dephosphorylates eIF2-alpha By similarity. Binds to proliferating cell nuclear antigen (PCNA), which may release host cells from growth arrest and facilitate viral replication. Ref.5

Subcellular location

Host cytoplasm By similarity. Host nucleushost nucleolus By similarity. Virion By similarity. Note: At early times in infection, colocalizes with PCNA and replication proteins in cell nuclei, before accumulating in the cytoplasm by 8 to 12 hours post-infection. The effects on the host cell are probably mediated by de novo-synthesized ICP34.5, the virion-derived population being either non-functional or present in very low amounts.

Domain

The triplet repeats region may play a role in modulating virus egress By similarity.

Miscellaneous

ICP34.5 is detected as early as 3 hpi prior to viral replication but reaches maximal levels late in infection. ICP34.5 gene is therefore classified as gamma-1 or leaky late gene.

The phosphatase activity of the ICP34.5-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, which inhibits viral replication By similarity.

Sequence similarities

Belongs to the PPP1R15 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PPP1CAP621364EBI-6149234,EBI-357253From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Neurovirulence factor ICP34.5
PRO_0000115806

Regions

Repeat161 – 16331
Repeat164 – 16632
Repeat167 – 16933
Repeat170 – 17234
Repeat173 – 17535
Region1 – 1616Required for nucleolar localization By similarity
Region161 – 175155 X 3 AA tandem repeats of A-T-P
Region175 – 18814Binding to PP1CA By similarity
Region190 – 24859Important for interferon resistance By similarity
Motif128 – 13710Nuclear export signal By similarity
Motif200 – 21819Bipartite nuclear localization signal By similarity
Compositional bias3 – 86Poly-Arg
Compositional bias10 – 175166Pro-rich
Compositional bias75 – 817Poly-Asp

Sequences

Sequence LengthMass (Da)Tools
P36313 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: BF216520CB379478

FASTA24826,196
        10         20         30         40         50         60 
MARRRRHRGP RRPRPPGPTG AVPTAQSQVT STPNSEPAVR SAPAAAPPPP PAGGPPPSCS 

        70         80         90        100        110        120 
LLLRQWLHVP ESASDDDDDD DWPDSPPPEP APEARPTAAA PRPRPPPPGV GPGGGADPSH 

       130        140        150        160        170        180 
PPSRPFRLPP RLALRLRVTA EHLARLRLRR AGGEGAPEPP ATPATPATPA TPATPARVRF 

       190        200        210        220        230        240 
SPHVRVRHLV VWASAARLAR RGSWARERAD RARFRRRVAE AEAVIGPCLG PEARARALAR 


GAGPANSV 

« Hide

References

[1]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Status of the ICP34.5 gene in herpes simplex virus type 1 strain 17."
Dolan A., McKie E., MacLean A.R., McGeoch D.J.
J. Gen. Virol. 73:971-973(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"The DNA sequences of the long repeat region and adjoining parts of the long unique region in the genome of herpes simplex virus type 1."
Perry L.J., McGeoch D.J.
J. Gen. Virol. 69:2831-2846(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Cell type and cell state determine differential in vitro growth of non-neurovirulent ICP34.5-negative herpes simplex virus types 1 and 2."
Brown S.M., Harland J., MacLean A.R., Podlech J., Clements J.B.
J. Gen. Virol. 75:2367-2377(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: 17syn+ and 17termA.
[5]"The herpes simplex virus virulence factor ICP34.5 and the cellular protein MyD116 complex with proliferating cell nuclear antigen through the 63-amino-acid domain conserved in ICP34.5, MyD116, and GADD34."
Brown S.M., MacLean A.R., McKie E.A., Harland J.
J. Virol. 71:9442-9449(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PCNA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32285.1.
X14112 Genomic DNA. Translation: CAA32348.1.
PIRJQ1682.
RefSeqNP_044600.1. NC_001806.1.
NP_044661.1. NC_001806.1.

3D structure databases

ProteinModelPortalP36313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid971431. 12 interactions.
IntActP36313. 13 interactions.
MINTMINT-6175580.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703395.
2703396.

Family and domain databases

InterProIPR019523. Prot_Pase1_reg-su15A/B_C.
[Graphical view]
PfamPF10488. PP1c_bdg. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameICP34_HHV11
AccessionPrimary (citable) accession number: P36313
Secondary accession number(s): O12396
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 26, 2008
Last modified: June 11, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families