ID MVP_TSWV1 Reviewed; 301 AA. AC P36292; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-1994, sequence version 1. DT 08-NOV-2023, entry version 64. DE RecName: Full=Movement protein; DE Short=MVP; DE AltName: Full=Non-structural movement protein; DE Short=NSm; GN Name=NSM; OS Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV). OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; OC Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; OC Tomato spotted wilt virus. OX NCBI_TaxID=36413; OH NCBI_TaxID=133901; Frankliniella occidentalis (Western flower thrips) (Euthrips occidentalis). OH NCBI_TaxID=163899; Scirtothrips dorsalis (Chilli thrips). OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum). OH NCBI_TaxID=161014; Thrips tabaci. RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RX PubMed=1431808; DOI=10.1099/0022-1317-73-11-2795; RA Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.; RT "The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, RT a bunyavirus with two ambisense RNA segments."; RL J. Gen. Virol. 73:2795-2804(1992). RN [2] RP ERRATUM OF PUBMED:1431808. RX PubMed=8468562; DOI=10.1099/0022-1317-74-4-790; RA Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.; RL J. Gen. Virol. 74:790-790(1993). RN [3] RP CHARACTERIZATION, AND SUBCELLULAR LOCATION. RX PubMed=8128638; DOI=10.1006/viro.1994.1162; RA Kormelink R., Storms M., Van Lent J., Peters D., Goldbach R.; RT "Expression and subcellular location of the NSM protein of tomato spotted RT wilt virus (TSWV), a putative viral movement protein."; RL Virology 200:56-65(1994). RN [4] RP FUNCTION. RX PubMed=8553550; DOI=10.1006/viro.1995.0059; RA Storms M.M., Kormelink R., Peters D., Van Lent J.W., Goldbach R.W.; RT "The nonstructural NSm protein of tomato spotted wilt virus induces tubular RT structures in plant and insect cells."; RL Virology 214:485-493(1995). RN [5] RP FUNCTION. RX PubMed=10640565; DOI=10.1099/0022-1317-81-1-257; RA Melcher U.; RT "The '30K' superfamily of viral movement proteins."; RL J. Gen. Virol. 81:257-266(2000). RN [6] RP FUNCTION. RX PubMed=16112159; DOI=10.1016/j.virol.2005.06.050; RA Lewandowski D.J., Adkins S.; RT "The tubule-forming NSm protein from Tomato spotted wilt virus complements RT cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids."; RL Virology 342:26-37(2005). RN [7] RP INTERACTION WITH HOST ARABIDOPSIS THALIANA AT4G26020 PROTEIN. RX PubMed=16903353; DOI=10.1094/mpmi-19-0874; RA Paape M., Solovyev A.G., Erokhina T.N., Minina E.A., Schepetilnikov M.V., RA Lesemann D.-E., Schiemann J., Morozov S.Y., Kellmann J.-W.; RT "At-4/1, an interactor of the Tomato spotted wilt virus movement protein, RT belongs to a new family of plant proteins capable of directed intra- and RT intercellular trafficking."; RL Mol. Plant Microbe Interact. 19:874-883(2006). RN [8] RP FUNCTION. RX PubMed=19481775; DOI=10.1016/j.virol.2009.04.027; RA Li W., Lewandowski D.J., Hilf M.E., Adkins S.; RT "Identification of domains of the Tomato spotted wilt virus NSm protein RT involved in tubule formation, movement and symptomatology."; RL Virology 390:110-121(2009). CC -!- FUNCTION: Viral movemement protein which is responsible for cell-to CC cell spread of viral genome. Increases the size exclusion limit (SEL) CC of plasmodesmata by forming tubules structures, thereby allowing viral CC ribonucleocapsids to spread directly to neighboring cells. CC {ECO:0000269|PubMed:10640565, ECO:0000269|PubMed:16112159, CC ECO:0000269|PubMed:19481775, ECO:0000269|PubMed:8553550}. CC -!- SUBUNIT: Oligomerizes to form tubule structures through host CC plasmodesma. Interacts with host A.thaliana At4g26020 protein, which is CC involved in intra- and inter-cellular trafficking. CC {ECO:0000269|PubMed:16903353}. CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma CC {ECO:0000269|PubMed:8128638}. Note=Colocalizes with viral nucleocapsid CC aggregates in host cytoplasm. CC -!- SIMILARITY: Belongs to the tospovirus movement protein family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; S48091; -; NOT_ANNOTATED_CDS; Genomic_RNA. DR PIR; JQ1927; JQ1927. DR Proteomes; UP000006674; Genome. DR GO; GO:0044219; C:host cell plasmodesma; IDA:UniProtKB. DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW. DR InterPro; IPR006889; Bunya_NSM. DR InterPro; IPR000603; MPV. DR Pfam; PF00803; 3A; 1. DR PIRSF; PIRSF003959; NS-M_TospoV; 1. PE 1: Evidence at protein level; KW Host cell junction; Host-virus interaction; Reference proteome; Transport; KW Viral movement protein. FT CHAIN 1..301 FT /note="Movement protein" FT /id="PRO_0000221987" FT REGION 1..22 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 19..159 FT /note="Essential for tubule formation and cell-to-cell FT movement" FT REGION 209..283 FT /note="Essential for tubule formation and cell-to-cell FT movement" FT REGION 282..301 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 284..288 FT /note="Essential for long-distance movement" SQ SEQUENCE 301 AA; 33564 MW; B00E16BEE6BA84C6 CRC64; MLTLFGNKRP SKSAGKDEGP LVSLAKHNGS VEVSKPWSSS DEKLALTKAM DASKGKILLN IEGTSSFGTY ESDSIIESEG YDLSARMIVD TNHHISNWKN DLFVGNGKQN ANKVIKICPT WDSRKQYMMI SRIVIWVCPT IPNPTGKLVV ALIDPNMPSG KQVILKGQGT ITDPICFVFY LNWSIPKMNN TPENCCQLHL MCSQEYKKGV SFGSVMYSWT KEFGDSPRAD KDKCMVIPLN RAIRARSQAF IEACKLIIPK CNSEKQIKKQ LKELSSNLER SVEEEEEGIS DSVAQLSFDE I //