P36292 (MVP_TSWV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Movement protein
Short name=MVP

Alternative name(s):
Non-structural movement protein
Short name=NSm

Gene names

Name:

NSM

Organism

Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV) [Reference proteome]

Taxonomic identifier

36413 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Tospovirus › Tomato spotted wilt virus

Virus host

Frankliniella occidentalis (Western flower thrips) [TaxID: 133901]
Scirtothrips dorsalis [TaxID: 163899]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Thrips tabaci [TaxID: 161014]

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Viral movemement protein which is responsible for cell-to cell spread of viral genome. Increases the size exclusion limit (SEL) of plasmodesmata by forming tubules structures, thereby allowing viral ribonucleocapsids to spread directly to neighboring cells. Ref.4 Ref.5 Ref.6 Ref.8
Subunit structure	Oligomerizes to form tubule structures through host plasmodesma. Interacts with host A.thaliana At4g26020 protein, which is involved in intra- and inter-cellular trafficking. Ref.7
Subcellular location	Host cell junction › host plasmodesma. Note: Colocalizes with viral nucleocapsid aggregates in host cytoplasm. Ref.3
Sequence similarities	Belongs to the tospovirus movement protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Transport
Cellular component	Host cell junction
Molecular function	Viral movement protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	spread of virus in host, cell to cell Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell plasmodesma Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Movement protein		PRO_0000221987
Regions
	Region	19 – 159	141	Essential for tubule formation and cell-to-cell movement
	Region	209 – 283	75	Essential for tubule formation and cell-to-cell movement
	Region	284 – 288	5	Essential for long-distance movement

Sequences

	Sequence		Length	Mass (Da)
	P36292 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B00E16BEE6BA84C6	FASTA	301	33,564
	10 20 30 40 50 60 MLTLFGNKRP SKSAGKDEGP LVSLAKHNGS VEVSKPWSSS DEKLALTKAM DASKGKILLN 70 80 90 100 110 120 IEGTSSFGTY ESDSIIESEG YDLSARMIVD TNHHISNWKN DLFVGNGKQN ANKVIKICPT 130 140 150 160 170 180 WDSRKQYMMI SRIVIWVCPT IPNPTGKLVV ALIDPNMPSG KQVILKGQGT ITDPICFVFY 190 200 210 220 230 240 LNWSIPKMNN TPENCCQLHL MCSQEYKKGV SFGSVMYSWT KEFGDSPRAD KDKCMVIPLN 250 260 270 280 290 300 RAIRARSQAF IEACKLIIPK CNSEKQIKKQ LKELSSNLER SVEEEEEGIS DSVAQLSFDE I

References

[1]	"The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments." Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R. J. Gen. Virol. 73:2795-2804(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	Erratum Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R. J. Gen. Virol. 74:790-790(1993) [PubMed] [Europe PMC] [Abstract]
[3]	"Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein." Kormelink R., Storms M., Van Lent J., Peters D., Goldbach R. Virology 200:56-65(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
[4]	"The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells." Storms M.M., Kormelink R., Peters D., Van Lent J.W., Goldbach R.W. Virology 214:485-493(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"The '30K' superfamily of viral movement proteins." Melcher U. J. Gen. Virol. 81:257-266(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids." Lewandowski D.J., Adkins S. Virology 342:26-37(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"At-4/1, an interactor of the Tomato spotted wilt virus movement protein, belongs to a new family of plant proteins capable of directed intra- and intercellular trafficking." Paape M., Solovyev A.G., Erokhina T.N., Minina E.A., Schepetilnikov M.V., Lesemann D.-E., Schiemann J., Morozov S.Y., Kellmann J.-W. Mol. Plant Microbe Interact. 19:874-883(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HOST ARABIDOPSIS THALIANA AT4G26020 PROTEIN.
[8]	"Identification of domains of the Tomato spotted wilt virus NSm protein involved in tubule formation, movement and symptomatology." Li W., Lewandowski D.J., Hilf M.E., Adkins S. Virology 390:110-121(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S48091 Genomic RNA. No translation available.
PIR	JQ1927.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000603. 3A/RNA2_movement_prot. IPR006889. Bunya_NSM. [Graphical view]
Pfam	PF00803. 3A. 1 hit. [Graphical view]
PIRSF	PIRSF003959. NS-M_TospoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MVP_TSWV1

Accession

Primary (citable) accession number: P36292

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program