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P36291

- GP_TSWV1

UniProt

P36291 - GP_TSWV1

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Protein

Envelope glycoprotein

Gene

GP

Organism
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei484 – 4852Cleavage; by host signal peptidaseBy similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. modulation by virus of host morphology or physiology Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiTomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
Taxonomic identifieri36413 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostiFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
Scirtothrips dorsalis [TaxID: 163899]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Thrips tabaci [TaxID: 161014]
ProteomesiUP000006674: Genome

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.
Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell membrane Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Chaini36 – 11351100Envelope glycoproteinPRO_0000036859Add
BLAST
Chaini36 – 484449Glycoprotein G1PRO_0000036860Add
BLAST
Chaini485 – 1135651Glycoprotein G2PRO_0000036861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi210 – 2101N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi340 – 3401N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi605 – 6051N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi980 – 9801N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.1 Publication
Glycosylated. Glycosylation is essential for proper subcellular location.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.

Structurei

3D structure databases

ProteinModelPortaliP36291.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 345310LumenalSequence AnalysisAdd
BLAST
Topological domaini367 – 484118CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini485 – 1067583LumenalSequence AnalysisAdd
BLAST
Topological domaini1089 – 113547CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei346 – 36621HelicalSequence AnalysisAdd
BLAST
Transmembranei1068 – 108821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 433Cell attachment siteSequence Analysis

Domaini

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36291-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MRILKLLELV VKVSLFTIAL SSVLLAFLIF RATDAKVEII RGDHPEIYDD
60 70 80 90 100
SAENEVPTAA SIQREAILET LTNLMLESRT PGTRQIREEK STIPISAEPT
110 120 130 140 150
TQKTISVLDL PNNCLNASSL KCEIKGISTY NVYYQVENNG VIYSCVSDSA
160 170 180 190 200
EGLEKCDNSL NLPKRFSKVP VIPITKLDKK RHFSVGGKFF ISESLTQDNY
210 220 230 240 250
PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT SPEKIMGYLI
260 270 280 290 300
KKPGENVEHK VISFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR
310 320 330 340 350
DCIIKYSKSI YKQTACINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY
360 370 380 390 400
DLMGLITYPV LLLINCLWKY FPLKCSNCGN LCIVTHECTK VCICNKSKAS
410 420 430 440 450
KEHSSECPIL SKEADHDYNK HKWTSMEWFH LIVNTKLSLS LLKFVTEILI
460 470 480 490 500
GLVILSQMPM SMAQTTQCLS GCFYVPGCPF LVTSKFEKCS EKDQCYCNVK
510 520 530 540 550
EDKIIESIFG TNIVIEGPND CIENQNCIAR PSIDNLIKCR LGCEYLDLFR
560 570 580 590 600
NKPLYNGFSD YTGSSLGLTS VGLYEAKRLR NGIIDSYNRQ GKISGMVAGD
610 620 630 640 650
SLNKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK
660 670 680 690 700
TSETAKKFVI YIKSVGIHYE VSEKYTTAPI QSTHTDFYST CTGNCDTCRK
710 720 730 740 750
NQALTGFQDF CVTPTSYWGC EEAWCFAINE GATCGFCRNI YDMDKSYRIY
760 770 780 790 800
SVLKSTIVAD VCISGILGGQ CSRITEEVPY ENTLFQADIQ ADLHNDGITI
810 820 830 840 850
GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTHDGVPIF TKKTLEGDDM
860 870 880 890 900
SWDCAAIGKK SVTIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLAKSF
910 920 930 940 950
SLGKLKMVVD LPSDLFKVAP KKPSITSTSL NCNGCLLCGQ GLSCLLEFFS
960 970 980 990 1000
DLTFSTAISI DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG
1010 1020 1030 1040 1050
NPDISVEVLV NNVIVEEPEN IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS
1060 1070 1080 1090 1100
PFNFIASYFG SFFDTIRVVL LIAFIFLVTY FCSILTSICK GYVKNESYKS
1110 1120 1130
RSKIEDDDEP EIKAPMLMKD TMTRRRPPMD FSHLV
Length:1,135
Mass (Da):127,318
Last modified:June 1, 1994 - v1
Checksum:iAC1A3FFFE84044FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S48091 Genomic RNA. Translation: AAB24089.1.
PIRiJQ1928.
RefSeqiNP_049359.1. NC_002050.1.

Genome annotation databases

GeneIDi956579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S48091 Genomic RNA. Translation: AAB24089.1 .
PIRi JQ1928.
RefSeqi NP_049359.1. NC_002050.1.

3D structure databases

ProteinModelPortali P36291.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956579.

Family and domain databases

InterProi IPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view ]
Pfami PF03557. Bunya_G1. 1 hit.
[Graphical view ]
PIRSFi PIRSF003960. M_poly_TospoV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments."
    Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
    J. Gen. Virol. 73:2795-2804(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Erratum
    Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
    J. Gen. Virol. 74:790-790(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells."
    Kikkert M., Verschoor A., Kormelink R., Rottier P., Goldbach R.
    J. Virol. 75:1004-1012(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, POST-TRANSLATIONAL MODIFICATIONS, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGP_TSWV1
AccessioniPrimary (citable) accession number: P36291
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3