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P36291 (GP_TSWV1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Envelope glycoprotein

Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 2 chains:

  1. Glycoprotein G1
  2. Glycoprotein G2
Gene names
Name:GP
OrganismTomato spotted wilt virus (strain Brazilian Br-01) (TSWV) [Reference proteome]
Taxonomic identifier36413 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
Scirtothrips dorsalis [TaxID: 163899]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Thrips tabaci [TaxID: 161014]

Protein attributes

Sequence length1135 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.

Subunit structure

G1 and G2 interacts with each other.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs. Ref.3

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal. Ref.3

Domain

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.

Glycosylated. Glycosylation is essential for proper subcellular location.

Sequence similarities

Belongs to the tospovirus envelope glycoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3535 Potential
Chain36 – 11351100Envelope glycoprotein
PRO_0000036859
Chain36 – 484449Glycoprotein G1
PRO_0000036860
Chain485 – 1135651Glycoprotein G2
PRO_0000036861

Regions

Topological domain36 – 345310Lumenal Potential
Transmembrane346 – 36621Helical; Potential
Topological domain367 – 484118Cytoplasmic Potential
Topological domain485 – 1067583Lumenal Potential
Transmembrane1068 – 108821Helical; Potential
Topological domain1089 – 113547Cytoplasmic Potential
Motif41 – 433Cell attachment site Potential

Sites

Site484 – 4852Cleavage; by host signal peptidase By similarity

Amino acid modifications

Glycosylation1161N-linked (GlcNAc...); by host Potential
Glycosylation2101N-linked (GlcNAc...); by host Potential
Glycosylation3401N-linked (GlcNAc...); by host Potential
Glycosylation6051N-linked (GlcNAc...); by host Potential
Glycosylation9801N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P36291 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: AC1A3FFFE84044FB

FASTA1,135127,318
        10         20         30         40         50         60 
MRILKLLELV VKVSLFTIAL SSVLLAFLIF RATDAKVEII RGDHPEIYDD SAENEVPTAA 

        70         80         90        100        110        120 
SIQREAILET LTNLMLESRT PGTRQIREEK STIPISAEPT TQKTISVLDL PNNCLNASSL 

       130        140        150        160        170        180 
KCEIKGISTY NVYYQVENNG VIYSCVSDSA EGLEKCDNSL NLPKRFSKVP VIPITKLDKK 

       190        200        210        220        230        240 
RHFSVGGKFF ISESLTQDNY PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT 

       250        260        270        280        290        300 
SPEKIMGYLI KKPGENVEHK VISFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR 

       310        320        330        340        350        360 
DCIIKYSKSI YKQTACINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY DLMGLITYPV 

       370        380        390        400        410        420 
LLLINCLWKY FPLKCSNCGN LCIVTHECTK VCICNKSKAS KEHSSECPIL SKEADHDYNK 

       430        440        450        460        470        480 
HKWTSMEWFH LIVNTKLSLS LLKFVTEILI GLVILSQMPM SMAQTTQCLS GCFYVPGCPF 

       490        500        510        520        530        540 
LVTSKFEKCS EKDQCYCNVK EDKIIESIFG TNIVIEGPND CIENQNCIAR PSIDNLIKCR 

       550        560        570        580        590        600 
LGCEYLDLFR NKPLYNGFSD YTGSSLGLTS VGLYEAKRLR NGIIDSYNRQ GKISGMVAGD 

       610        620        630        640        650        660 
SLNKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK TSETAKKFVI 

       670        680        690        700        710        720 
YIKSVGIHYE VSEKYTTAPI QSTHTDFYST CTGNCDTCRK NQALTGFQDF CVTPTSYWGC 

       730        740        750        760        770        780 
EEAWCFAINE GATCGFCRNI YDMDKSYRIY SVLKSTIVAD VCISGILGGQ CSRITEEVPY 

       790        800        810        820        830        840 
ENTLFQADIQ ADLHNDGITI GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTHDGVPIF 

       850        860        870        880        890        900 
TKKTLEGDDM SWDCAAIGKK SVTIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLAKSF 

       910        920        930        940        950        960 
SLGKLKMVVD LPSDLFKVAP KKPSITSTSL NCNGCLLCGQ GLSCLLEFFS DLTFSTAISI 

       970        980        990       1000       1010       1020 
DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG NPDISVEVLV NNVIVEEPEN 

      1030       1040       1050       1060       1070       1080 
IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS PFNFIASYFG SFFDTIRVVL LIAFIFLVTY 

      1090       1100       1110       1120       1130 
FCSILTSICK GYVKNESYKS RSKIEDDDEP EIKAPMLMKD TMTRRRPPMD FSHLV 

« Hide

References

[1]"The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments."
Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
J. Gen. Virol. 73:2795-2804(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]Erratum
Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
J. Gen. Virol. 74:790-790(1993) [PubMed] [Europe PMC] [Abstract]
[3]"Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells."
Kikkert M., Verschoor A., Kormelink R., Rottier P., Goldbach R.
J. Virol. 75:1004-1012(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, POST-TRANSLATIONAL MODIFICATIONS, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S48091 Genomic RNA. Translation: AAB24089.1.
PIRJQ1928.
RefSeqNP_049359.1. NC_002050.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956579.

Family and domain databases

InterProIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFPIRSF003960. M_poly_TospoV. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGP_TSWV1
AccessionPrimary (citable) accession number: P36291
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families