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P36291

- GP_TSWV1

UniProt

P36291 - GP_TSWV1

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Protein
Envelope glycoprotein
Gene
GP
Organism
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei484 – 4852Cleavage; by host signal peptidase By similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. modulation by virus of host morphology or physiology Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiTomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
Taxonomic identifieri36413 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
Virus hostiFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
Scirtothrips dorsalis [TaxID: 163899]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Thrips tabaci [TaxID: 161014]
ProteomesiUP000006674: Genome

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host Golgi apparatus membrane; Single-pass type I membrane protein Reviewed prediction. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Reviewed prediction
Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.1 Publication
Chain Glycoprotein G2 : Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host Golgi apparatus membrane; Single-pass type I membrane protein Reviewed prediction
Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 345310Lumenal Reviewed prediction
Add
BLAST
Transmembranei346 – 36621Helical; Reviewed prediction
Add
BLAST
Topological domaini367 – 484118Cytoplasmic Reviewed prediction
Add
BLAST
Topological domaini485 – 1067583Lumenal Reviewed prediction
Add
BLAST
Transmembranei1068 – 108821Helical; Reviewed prediction
Add
BLAST
Topological domaini1089 – 113547Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell Golgi membrane Source: UniProtKB-SubCell
  2. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535 Reviewed prediction
Add
BLAST
Chaini36 – 11351100Envelope glycoprotein
PRO_0000036859Add
BLAST
Chaini36 – 484449Glycoprotein G1
PRO_0000036860Add
BLAST
Chaini485 – 1135651Glycoprotein G2
PRO_0000036861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi210 – 2101N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi340 – 3401N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi605 – 6051N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi980 – 9801N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.
Glycosylated. Glycosylation is essential for proper subcellular location.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.

Structurei

3D structure databases

ProteinModelPortaliP36291.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 433Cell attachment site Reviewed prediction

Domaini

The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
[Graphical view]
PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36291-1 [UniParc]FASTAAdd to Basket

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MRILKLLELV VKVSLFTIAL SSVLLAFLIF RATDAKVEII RGDHPEIYDD     50
SAENEVPTAA SIQREAILET LTNLMLESRT PGTRQIREEK STIPISAEPT 100
TQKTISVLDL PNNCLNASSL KCEIKGISTY NVYYQVENNG VIYSCVSDSA 150
EGLEKCDNSL NLPKRFSKVP VIPITKLDKK RHFSVGGKFF ISESLTQDNY 200
PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT SPEKIMGYLI 250
KKPGENVEHK VISFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR 300
DCIIKYSKSI YKQTACINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY 350
DLMGLITYPV LLLINCLWKY FPLKCSNCGN LCIVTHECTK VCICNKSKAS 400
KEHSSECPIL SKEADHDYNK HKWTSMEWFH LIVNTKLSLS LLKFVTEILI 450
GLVILSQMPM SMAQTTQCLS GCFYVPGCPF LVTSKFEKCS EKDQCYCNVK 500
EDKIIESIFG TNIVIEGPND CIENQNCIAR PSIDNLIKCR LGCEYLDLFR 550
NKPLYNGFSD YTGSSLGLTS VGLYEAKRLR NGIIDSYNRQ GKISGMVAGD 600
SLNKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK 650
TSETAKKFVI YIKSVGIHYE VSEKYTTAPI QSTHTDFYST CTGNCDTCRK 700
NQALTGFQDF CVTPTSYWGC EEAWCFAINE GATCGFCRNI YDMDKSYRIY 750
SVLKSTIVAD VCISGILGGQ CSRITEEVPY ENTLFQADIQ ADLHNDGITI 800
GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTHDGVPIF TKKTLEGDDM 850
SWDCAAIGKK SVTIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLAKSF 900
SLGKLKMVVD LPSDLFKVAP KKPSITSTSL NCNGCLLCGQ GLSCLLEFFS 950
DLTFSTAISI DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG 1000
NPDISVEVLV NNVIVEEPEN IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS 1050
PFNFIASYFG SFFDTIRVVL LIAFIFLVTY FCSILTSICK GYVKNESYKS 1100
RSKIEDDDEP EIKAPMLMKD TMTRRRPPMD FSHLV 1135
Length:1,135
Mass (Da):127,318
Last modified:June 1, 1994 - v1
Checksum:iAC1A3FFFE84044FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S48091 Genomic RNA. Translation: AAB24089.1.
PIRiJQ1928.
RefSeqiNP_049359.1. NC_002050.1.

Genome annotation databases

GeneIDi956579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S48091 Genomic RNA. Translation: AAB24089.1 .
PIRi JQ1928.
RefSeqi NP_049359.1. NC_002050.1.

3D structure databases

ProteinModelPortali P36291.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956579.

Family and domain databases

InterProi IPR005167. Bunya_G1.
IPR014414. M_poly_TospoV.
[Graphical view ]
Pfami PF03557. Bunya_G1. 1 hit.
[Graphical view ]
PIRSFi PIRSF003960. M_poly_TospoV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments."
    Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
    J. Gen. Virol. 73:2795-2804(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Erratum
    Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
    J. Gen. Virol. 74:790-790(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells."
    Kikkert M., Verschoor A., Kormelink R., Rottier P., Goldbach R.
    J. Virol. 75:1004-1012(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, POST-TRANSLATIONAL MODIFICATIONS, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGP_TSWV1
AccessioniPrimary (citable) accession number: P36291
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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