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P36291

- GP_TSWV1

UniProt

P36291 - GP_TSWV1

Protein

Envelope glycoprotein

Gene

GP

Organism
Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei484 – 4852Cleavage; by host signal peptidaseBy similarity

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. modulation by virus of host morphology or physiology Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein
    Short name:
    GP
    Alternative name(s):
    M polyprotein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:GP
    OrganismiTomato spotted wilt virus (strain Brazilian Br-01) (TSWV)
    Taxonomic identifieri36413 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeTospovirus
    Virus hostiFrankliniella occidentalis (Western flower thrips) [TaxID: 133901]
    Scirtothrips dorsalis [TaxID: 163899]
    Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
    Thrips tabaci [TaxID: 161014]
    ProteomesiUP000006674: Genome

    Subcellular locationi

    Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
    Note: Glycoprotein G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs.
    Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
    Note: Glycoprotein G2 is retained in the Golgi complex and probably contains a Golgi retention signal.

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 11351100Envelope glycoproteinPRO_0000036859Add
    BLAST
    Chaini36 – 484449Glycoprotein G1PRO_0000036860Add
    BLAST
    Chaini485 – 1135651Glycoprotein G2PRO_0000036861Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi210 – 2101N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi605 – 6051N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi980 – 9801N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins including glycoprotein G1 and glycoprotein G2.1 Publication
    Glycosylated. Glycosylation is essential for proper subcellular location.

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    G1 and G2 interacts with each other.

    Structurei

    3D structure databases

    ProteinModelPortaliP36291.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini36 – 345310LumenalSequence AnalysisAdd
    BLAST
    Topological domaini367 – 484118CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini485 – 1067583LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1089 – 113547CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei346 – 36621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1068 – 108821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi41 – 433Cell attachment siteSequence Analysis

    Domaini

    The cell attachment site present in these glycoproteins may help in the adhesion of virus to cells.

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR005167. Bunya_G1.
    IPR014414. M_poly_TospoV.
    [Graphical view]
    PfamiPF03557. Bunya_G1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003960. M_poly_TospoV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36291-1 [UniParc]FASTAAdd to Basket

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    MRILKLLELV VKVSLFTIAL SSVLLAFLIF RATDAKVEII RGDHPEIYDD     50
    SAENEVPTAA SIQREAILET LTNLMLESRT PGTRQIREEK STIPISAEPT 100
    TQKTISVLDL PNNCLNASSL KCEIKGISTY NVYYQVENNG VIYSCVSDSA 150
    EGLEKCDNSL NLPKRFSKVP VIPITKLDKK RHFSVGGKFF ISESLTQDNY 200
    PITYNSYPTN GTVSLQTVKL SGDCKITKSN FANPYTVSIT SPEKIMGYLI 250
    KKPGENVEHK VISFSGSASI TFTEEMLDGE HNLLCGDKSA KIPKTNKRVR 300
    DCIIKYSKSI YKQTACINFS WIRLILIALL IYFPIRWLVN KTTKPLFLWY 350
    DLMGLITYPV LLLINCLWKY FPLKCSNCGN LCIVTHECTK VCICNKSKAS 400
    KEHSSECPIL SKEADHDYNK HKWTSMEWFH LIVNTKLSLS LLKFVTEILI 450
    GLVILSQMPM SMAQTTQCLS GCFYVPGCPF LVTSKFEKCS EKDQCYCNVK 500
    EDKIIESIFG TNIVIEGPND CIENQNCIAR PSIDNLIKCR LGCEYLDLFR 550
    NKPLYNGFSD YTGSSLGLTS VGLYEAKRLR NGIIDSYNRQ GKISGMVAGD 600
    SLNKNETSIP ENILPRQSLI FDSVVDGKYR YMIEQSLLGG GGTIFMLNDK 650
    TSETAKKFVI YIKSVGIHYE VSEKYTTAPI QSTHTDFYST CTGNCDTCRK 700
    NQALTGFQDF CVTPTSYWGC EEAWCFAINE GATCGFCRNI YDMDKSYRIY 750
    SVLKSTIVAD VCISGILGGQ CSRITEEVPY ENTLFQADIQ ADLHNDGITI 800
    GELIAHGPDS HIYSGNIANL NDPVKMFGHP QLTHDGVPIF TKKTLEGDDM 850
    SWDCAAIGKK SVTIKTCGYD TYRFRSGLEQ ISDIPVSFKD FSSFFLAKSF 900
    SLGKLKMVVD LPSDLFKVAP KKPSITSTSL NCNGCLLCGQ GLSCLLEFFS 950
    DLTFSTAISI DACSLSTYQL AVKKGSNKYN ITMFCSANPD KKKMTLYPEG 1000
    NPDISVEVLV NNVIVEEPEN IIDQNDEYAH EEQQYNSDSS AWGFWDYIKS 1050
    PFNFIASYFG SFFDTIRVVL LIAFIFLVTY FCSILTSICK GYVKNESYKS 1100
    RSKIEDDDEP EIKAPMLMKD TMTRRRPPMD FSHLV 1135
    Length:1,135
    Mass (Da):127,318
    Last modified:June 1, 1994 - v1
    Checksum:iAC1A3FFFE84044FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S48091 Genomic RNA. Translation: AAB24089.1.
    PIRiJQ1928.
    RefSeqiNP_049359.1. NC_002050.1.

    Genome annotation databases

    GeneIDi956579.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S48091 Genomic RNA. Translation: AAB24089.1 .
    PIRi JQ1928.
    RefSeqi NP_049359.1. NC_002050.1.

    3D structure databases

    ProteinModelPortali P36291.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956579.

    Family and domain databases

    InterProi IPR005167. Bunya_G1.
    IPR014414. M_poly_TospoV.
    [Graphical view ]
    Pfami PF03557. Bunya_G1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003960. M_poly_TospoV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments."
      Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
      J. Gen. Virol. 73:2795-2804(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Erratum
      Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.
      J. Gen. Virol. 74:790-790(1993) [PubMed] [Europe PMC] [Abstract]
    3. "Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells."
      Kikkert M., Verschoor A., Kormelink R., Rottier P., Goldbach R.
      J. Virol. 75:1004-1012(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, POST-TRANSLATIONAL MODIFICATIONS, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGP_TSWV1
    AccessioniPrimary (citable) accession number: P36291
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3