P36252 (RPOC_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	TM_0459

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	1690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1690	1690	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067818

Sequences

	Sequence		Length	Mass (Da)
	P36252 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 9ED23FD2E9512CE6	FASTA	1,690	190,521
	10 20 30 40 50 60 MPMSSFKRKI KAIQIKIASP EVIRSWSGGE VKKPETINYR TFKPERDGLF CERIFGPVKD 70 80 90 100 110 120 YECACGKYKG KKYEGTVCER CGVRVESREA RRKRMGHIEL AAPAVHIWYL ESIPSVLGTL 130 140 150 160 170 180 LNMSTSDLEN IIYYGSRRVI ERAFIVTDPK DTPFSQGDVI YETEYRIYRK KWDFDVEQAF 190 200 210 220 230 240 VVKNPKSPVL SDIDGEVTLK TEKSITGREI TWIIVKNITR ATHTVLPGMI LVVKDGQEVE 250 260 270 280 290 300 KGQDLTKEMT IDPVYAPFDG HVEIDELSNT ITLKPLTTSK DQPVVFTIPY GAKILVSNGQ 310 320 330 340 350 360 KVKKGDQITT STSLPAVKAS ISGTVRFGSN LNIRALEDGN FEVLSTGEVY VEQVIEERKY 370 380 390 400 410 420 PVFEGALVYV NNGDQVKKGD HLADRFLFEE EYLSATEYKI FESHYPTMFD VEERTENDRP 430 440 450 460 470 480 IVVITDIDPE VSKETGLKVG DIVTENEYEA YLQIYPEKIV ADAGAQAIKK LLQNLDLEAL 490 500 510 520 530 540 QAEIEAELKK LPSSSSKAIK LRRRLKMVKD FLKSGNKPEW MVLEVVPVIP PDLRPMIQIE 550 560 570 580 590 600 GGRFATTDLN ELYRRLINRN NRLKKLLELG APEIILRNEK RMLQEAVDAL IHNGSDSEGK 610 620 630 640 650 660 RSRRAVLKDR NGRPLKSLTD LLKGKKGRFR RNLLGKRVDY SGRAVIVVGP NLKIHQCGIP 670 680 690 700 710 720 KKMAMELFKP FVLAKLLGEG SSSKTMRKVK KAIIEKEMPE AWEVLEEVIK GSVVLLNRAP 730 740 750 760 770 780 TLHRMSIQAF EPKLVEGNAI QLHPVVCPPF NADFDGDQMA VHVPLSAAAQ AEARFLMLSR 790 800 810 820 830 840 YNIISPAHGK PISLPTQDII IGSYYLTTVG KEFDSLKEED VKWKFSSPEE AMLAYHLGFI 850 860 870 880 890 900 KLHTPILIKV AVNGEEKRIK TTLGRVIFNG ILPEDLRDYN RIFDKKQINA LVYETFKRHG 910 920 930 940 950 960 IDRAADLLDD IKDIGFHYAT VSGLTLSLKD LKIPPERDEI LRKTWEKVRI IEENYEKGFL 970 980 990 1000 1010 1020 TEEQRKSEII RLWMSVTEEI TKLTSKTLAE DPFNPIYMMV NSGARGNIDQ VKQLAGIRGL 1030 1040 1050 1060 1070 1080 MIKAYDPRSR EIKSKIFKGQ AIHEALTFDY PVDKNLREGV DILQFFISTY GARKGQVDTA 1090 1100 1110 1120 1130 1140 MNTSFAGYLT RRLVDVAQSV TVAEPDCGTH EGIRAMDLIK EGTVVEKMNE FLFGRVLARD 1150 1160 1170 1180 1190 1200 VLDPETKEVL KNPETGKEYT RNTMLTDDDA NFLASYKKMV DVVRYEEIDI TELSLPNMYA 1210 1220 1230 1240 1250 1260 EIAEPVGEYE EGTELTWDVI KAAKNEGKYR IKVKVYPVVG TVYAEEEPLY DKKGERQLLV 1270 1280 1290 1300 1310 1320 YQEVINEIVA KMLEENGIEK VSVRPDIIVR SPLTCESEYG VCAACYGMDL SNHKIVNVGE 1330 1340 1350 1360 1370 1380 AVGVVAAQSI GEPGTQLTMR TFHVGGVMGA SDIVSGLTTV EKTFEPYAFL REEKSGGKKE 1390 1400 1410 1420 1430 1440 IRKYYGSEAI LCEVDGFVKD IATDESGRTV IYIEDYAGNI HAYKVPKRAK VRVEKGQKVL 1450 1460 1470 1480 1490 1500 RGETLTSGAI VWWKLLELES EKGVMTAMNL LKIIKNAYVQ QGVSIHDKHF EIIFKQMLSM 1510 1520 1530 1540 1550 1560 ATIVDPGDSD YLPDQLVPLV DIKRFNREIL EGNAKVEENR KWVIGKTLAK RIITETEEGE 1570 1580 1590 1600 1610 1620 LVELAQKGDE VTEELLKKII EAGIKEIDVF EKDKVVTYQI LPKEPIKYKR RLLSLKKAAL 1630 1640 1650 1660 1670 1680 NYPGWLSAAA FEETAWVLTA AAIEGKVDPL IGLKENVIVG QLIPAGTGLD VFAGIQVEET 1690 PRAAVEEELA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits." Palm P., Schleper C., Arnold-Ammer I., Holz I., Meier T., Lottspeich F., Zillig W. Nucleic Acids Res. 21:4904-4908(1993) [PubMed: 8177738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72695 Genomic DNA. Translation: CAA51247.1. AE000512 Genomic DNA. Translation: AAD35544.1.
PIR	S41467.
RefSeq	NP_228269.1. NC_000853.1.
3D structure databases
ProteinModelPortal	P36252.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897490.
GenomeReviews	Gene locus TM_0459 in contig AE000512_GR.
KEGG	tma:TM0459.
NMPDR	fig|243274.1.peg.453.
PATRIC	23935815. VBITheMar51294_0466.
TIGR	TM_0459.
Phylogenomic databases
HOGENOM	HBG621785.
OMA	DINDKHI.
PhylomeDB	P36252.
ProtClustDB	PRK00566.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0459-MONOMER.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 2 hits. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOC_THEMA

Accession

Primary (citable) accession number: P36252

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program