Reviewed, UniProtKB/Swiss-Prot P36230 (LECA_IRIHO)

Names and origin

Protein names	Recommended name:     N-acetyl-D-galactosamine-binding lectin subunit A     EC=3.2.2.22 Alternative name(s):     A-disaccharide-binding lectin subunit A     rRNA N-glycosidase
Organism	Iris hollandica (Dutch iris)
Taxonomic identifier	35876 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Iridaceae › Iris

Protein attributes

Sequence length	28 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Gal / GalNAc-specific lectin. Agglutinates both native and trypsin-treated rabbit erythrocytes but not human erythrocytes irrespective of blood group type.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Disulfide-linked heterodimer of A and B chains.
Sequence similarities	Belongs to the ribosome-inactivating protein family.

Ontologies

Keywords
Ligand	Lectin
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›28	›28	N-acetyl-D-galactosamine-binding lectin subunit A		PRO_0000221422
Experimental info
	Non-terminal residue	28	1

Sequences

	Sequence		Length	Mass (Da)
	P36230-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 6D806E619B97E05D	FASTA	28	3,270
	10 20 YDKVEFHITG XTKDTYSAFI QSLRTHLS

References

[1]

"Isolation and characterization of an N-acetyl-D-galactosamine-binding lectin from Dutch Iris bulbs which recognizes the blood group A disaccharide (GalNAc alpha 1-3Gal)." Mo H., van Damme E.J.M., Peumans W.J., Goldstein I.J. J. Biol. Chem. 269:7666-7673(1994) [PubMed: 8125993] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: cv. Golden Harvest and cv. Prof. Blaauw. Tissue: Bulb.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 119533.
Family and domain databases
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LECA_IRIHO

Accession

Primary (citable) accession number: P36230

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 19, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)