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Protein

Thymidine kinase

Gene

TK

Organism
Bovine herpesvirus 5 (strain N569) (BoHV-5) (Bovine encephalitis herpesvirus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Proton acceptorUniRule annotation1
Binding sitei88SubstrateUniRule annotation1
Binding sitei178ATPUniRule annotation1
Binding sitei184SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
OrganismiBovine herpesvirus 5 (strain N569) (BoHV-5) (Bovine encephalitis herpesvirus)
Taxonomic identifieri36350 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001750641 – 350Thymidine kinaseAdd BLAST350

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP36226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 35Poly-Ala9
Compositional biasi315 – 318Poly-Arg4

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P36226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPALRVVR VYLDGAHGLG KTTTGRALAA AASAAGGPVL FFPEPMAYWR
60 70 80 90 100
TMFPTDALSG ILAASARRAA AQGGRADADA AGLVAYYQAR FAAPYLVLHA
110 120 130 140 150
RVAALLAPPE PAPGGDDVVT LVFDRHPLAA CLCYPFARYC LREINAEDLL
160 170 180 190 200
ALAATAPLEA PGANLVVCTL PPAEQQRRLA ARARPGDRAD AGFWPPLRNA
210 220 230 240 250
YALLANTCAF LGAGGAWRDG WDALGWADAD ALAALADPRG GGREPVPAPA
260 270 280 290 300
LRDTLFAALK CRELYPGGGA DLPAVHAWAL DALADRLAAL EVFMDVSAAP
310 320 330 340 350
DACAAAVLGM RPAMRRRRAD GAAGATLADL ARRFAREMTP GGPEAAPRGL
Length:350
Mass (Da):36,263
Last modified:June 1, 1994 - v1
Checksum:iBFBECC84E839919D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S56149 Genomic DNA. Translation: AAB19542.1.
PIRiA45663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S56149 Genomic DNA. Translation: AAB19542.1.
PIRiA45663.

3D structure databases

ProteinModelPortaliP36226.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_BHV5
AccessioniPrimary (citable) accession number: P36226
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.