Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P36224 (JNM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear migration protein JNM1
Gene names
Name:JNM1
Synonyms:INS1
Ordered Locus Names:YMR294W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding By similarity. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. JNM1 is associated with the rod and links it to the projecting sidearm. Required for proper nuclear migration during the mitotic cell cycle and for astral microtubule development. Ref.4 Ref.5

Subunit structure

Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of ARP1 polymers attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod structure is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding By similarity. Interacts with ARP1. Ref.4 Ref.6 Ref.7

Subcellular location

Cytoplasmcytoskeleton Probable.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Nuclear migration protein JNM1
PRO_0000084289

Regions

Coiled coil114 – 13926 Potential
Coiled coil200 – 24546 Potential
Coiled coil331 – 36737 Potential

Amino acid modifications

Modified residue911Phosphoserine Ref.8

Experimental info

Sequence conflict921N → S in CAA81023. Ref.1
Sequence conflict1141K → R in CAA81023. Ref.1
Sequence conflict1931P → A in CAA81023. Ref.1
Sequence conflict2011D → E in CAA81023. Ref.1
Sequence conflict2641S → N in CAA81023. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P36224 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 80646B78B484AE56

FASTA37343,607
        10         20         30         40         50         60 
MNVIDLSDPA INVDYDSLIG IDNEESQEIF ENEVKEDGQQ EEQEEASSRK DGLIVEPGRD 

        70         80         90        100        110        120 
VESLRRAIRD QLLFKIHRQN QSDCADARKL SNDEEDESRQ QKLERIREEL EELKIENLTS 

       130        140        150        160        170        180 
EMQTEIKELC EIQSKLATES SSRLTNLRKK LLETYEGQDT VILPNIILDT SNIKRLQKLD 

       190        200        210        220        230        240 
QKISLMERFV GIPEALEAEE DRKSVHSKVN ELYRSIQLLQ GDDKAEGKLQ KFRDRLVELN 

       250        260        270        280        290        300 
EEFENSLLGK KIQQDLRLKD DTVSKLVMPE NKVKEINSMY SMFKQYQDSL PLLAERMKSL 

       310        320        330        340        350        360 
NKMNNRVIEV YETTKGLDSQ ITSIQEQGKV WLKALNELDK KFDEQEVKIR ENMEQIRRKI 

       370 
DTLEDEALQR NSK 

« Hide

References

« Hide 'large scale' references
[1]"The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle."
McMillan J.N., Tatchell K.
J. Cell Biol. 125:143-158(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B."
Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A.
Mol. Biol. Cell 9:1741-1756(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, FUNCTION OF THE DYNACTIN COMPLEX.
[5]"Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae."
Suzuki M., Igarashi R., Sekiya M., Utsugi T., Morishita S., Yukawa M., Ohya Y.
Nat. Cell Biol. 6:861-871(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE DYNACTIN COMPLEX.
[6]"Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued."
Clark S.W., Rose M.D.
Mol. Biol. Cell 16:3999-4012(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARP1.
[7]"Arp10p is a pointed-end-associated component of yeast dynactin."
Clark S.W., Rose M.D.
Mol. Biol. Cell 17:738-748(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SELF-ASSOCIATION, INTERACTION WITH ARP1 AND ARP10.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z25750 Genomic DNA. Translation: CAA81023.1.
X80836 Genomic DNA. Translation: CAA56803.1.
BK006946 Genomic DNA. Translation: DAA10195.1.
PIRS47455.
RefSeqNP_014022.1. NM_001182802.1.

3D structure databases

ProteinModelPortalP36224.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35474. 287 interactions.
DIPDIP-2387N.
IntActP36224. 9 interactions.
MINTMINT-493774.
STRING4932.YMR294W.

Proteomic databases

MaxQBP36224.
PaxDbP36224.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR294W; YMR294W; YMR294W.
GeneID855339.
KEGGsce:YMR294W.

Organism-specific databases

CYGDYMR294w.
SGDS000004908. JNM1.

Phylogenomic databases

eggNOGNOG285417.
OMADAGHCER.
OrthoDBEOG7J189X.

Enzyme and pathway databases

BioCycYEAST:G3O-32964-MONOMER.

Gene expression databases

GenevestigatorP36224.

Family and domain databases

InterProIPR028133. Dynamitin.
[Graphical view]
PANTHERPTHR15346:SF0. PTHR15346:SF0. 1 hit.
ProtoNetSearch...

Other

NextBio979076.

Entry information

Entry nameJNM1_YEAST
AccessionPrimary (citable) accession number: P36224
Secondary accession number(s): D6W0C1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD