P36224 (JNM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear migration protein JNM1

Gene names

Name:	JNM1
Synonyms:	INS1
Ordered Locus Names:	YMR294W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding By similarity. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. JNM1 is associated with the rod and links it to the projecting sidearm. Required for proper nuclear migration during the mitotic cell cycle and for astral microtubule development. Ref.4 Ref.5
Subunit structure	Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of ARP1 polymers attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod structure is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding By similarity. Interacts with ARP1. Ref.4 Ref.6 Ref.7
Subcellular location	Cytoplasm › cytoskeleton Probable.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Dynein Microtubule
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of mitotic spindle orientation Inferred from mutant phenotype Ref.1. Source: SGD metabolic process Inferred from electronic annotation. Source: GOC mitosis Inferred from electronic annotation. Source: InterPro nuclear migration Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW dynactin complex Inferred from direct assay Ref.4. Source: SGD dynein complex Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of cytoskeleton Inferred from physical interaction Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARP1	P38696	9	EBI-9415,EBI-2920
ARP10	Q04549	3	EBI-9415,EBI-2977
LDB18	Q07887	4	EBI-9415,EBI-33243
NIP100	P33420	3	EBI-9415,EBI-12049

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Nuclear migration protein JNM1		PRO_0000084289
Regions
	Coiled coil	114 – 139	26	Potential
	Coiled coil	200 – 245	46	Potential
	Coiled coil	331 – 367	37	Potential
Amino acid modifications
	Modified residue	91	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	92	1	N → S in CAA81023. Ref.1
	Sequence conflict	114	1	K → R in CAA81023. Ref.1
	Sequence conflict	193	1	P → A in CAA81023. Ref.1
	Sequence conflict	201	1	D → E in CAA81023. Ref.1
	Sequence conflict	264	1	S → N in CAA81023. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36224 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 80646B78B484AE56	FASTA	373	43,607
	10 20 30 40 50 60 MNVIDLSDPA INVDYDSLIG IDNEESQEIF ENEVKEDGQQ EEQEEASSRK DGLIVEPGRD 70 80 90 100 110 120 VESLRRAIRD QLLFKIHRQN QSDCADARKL SNDEEDESRQ QKLERIREEL EELKIENLTS 130 140 150 160 170 180 EMQTEIKELC EIQSKLATES SSRLTNLRKK LLETYEGQDT VILPNIILDT SNIKRLQKLD 190 200 210 220 230 240 QKISLMERFV GIPEALEAEE DRKSVHSKVN ELYRSIQLLQ GDDKAEGKLQ KFRDRLVELN 250 260 270 280 290 300 EEFENSLLGK KIQQDLRLKD DTVSKLVMPE NKVKEINSMY SMFKQYQDSL PLLAERMKSL 310 320 330 340 350 360 NKMNNRVIEV YETTKGLDSQ ITSIQEQGKV WLKALNELDK KFDEQEVKIR ENMEQIRRKI 370 DTLEDEALQR NSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle." McMillan J.N., Tatchell K. J. Cell Biol. 125:143-158(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B." Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A. Mol. Biol. Cell 9:1741-1756(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, FUNCTION OF THE DYNACTIN COMPLEX.
[5]	"Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae." Suzuki M., Igarashi R., Sekiya M., Utsugi T., Morishita S., Yukawa M., Ohya Y. Nat. Cell Biol. 6:861-871(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE DYNACTIN COMPLEX.
[6]	"Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued." Clark S.W., Rose M.D. Mol. Biol. Cell 16:3999-4012(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARP1.
[7]	"Arp10p is a pointed-end-associated component of yeast dynactin." Clark S.W., Rose M.D. Mol. Biol. Cell 17:738-748(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SELF-ASSOCIATION, INTERACTION WITH ARP1 AND ARP10.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25750 Genomic DNA. Translation: CAA81023.1. X80836 Genomic DNA. Translation: CAA56803.1. BK006946 Genomic DNA. Translation: DAA10195.1.
PIR	S47455.
RefSeq	NP_014022.1. NM_001182802.1.
3D structure databases
ProteinModelPortal	P36224.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35474. 287 interactions.
DIP	DIP-2387N.
IntAct	P36224. 9 interactions.
MINT	MINT-493774.
STRING	4932.YMR294W.
Proteomic databases
PaxDb	P36224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR294W; YMR294W; YMR294W.
GeneID	855339.
KEGG	sce:YMR294W.
Organism-specific databases
CYGD	YMR294w.
SGD	S000004908. JNM1.
Phylogenomic databases
eggNOG	NOG285417.
OMA	TEANEDI.
OrthoDB	EOG7J189X.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32964-MONOMER.
Gene expression databases
Genevestigator	P36224.
Family and domain databases
InterPro	IPR028133. Dynamitin. [Graphical view]
PANTHER	PTHR15346:SF0. PTHR15346:SF0. 1 hit.
ProtoNet	Search...
Other
NextBio	979076.

Entry information

Entry name

JNM1_YEAST

Accession

Primary (citable) accession number: P36224
Secondary accession number(s): D6W0C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	February 19, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program