Nuclear migration protein JNM1 - JNM1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.

Contribute

Send feedback

Read comments (?) or add your own

P36224 (JNM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nuclear migration protein JNM1

Gene names

Name:	JNM1
Synonyms:	INS1
Ordered Locus Names:	YMR294W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding By similarity. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. JNM1 is associated with the rod and links it to the projecting sidearm. Required for proper nuclear migration during the mitotic cell cycle and for astral microtubule development. Ref.4 Ref.5
Subunit structure	Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of ARP1 polymers attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod structure is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding By similarity. Interacts with ARP1. Ref.4 Ref.6 Ref.7
Subcellular location	Cytoplasm › cytoskeleton Probable.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Dynein Microtubule
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of mitotic spindle orientation Inferred from mutant phenotype Ref.1. Source: SGD mitotic nuclear division Inferred from electronic annotation. Source: InterPro nuclear migration Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW dynactin complex Inferred from direct assay Ref.4. Source: SGD dynein complex Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction PubMed 11283351 Ref.7 PubMed 18245366 PubMed 18719252. Source: IntAct structural constituent of cytoskeleton Inferred from physical interaction Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ARP1	P38696	9	EBI-9415,EBI-2920
ARP10	Q04549	3	EBI-9415,EBI-2977
LDB18	Q07887	4	EBI-9415,EBI-33243
NIP100	P33420	3	EBI-9415,EBI-12049

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 373

373

Nuclear migration protein JNM1

PRO_0000084289

Regions

Coiled coil

114 – 139

Potential

Coiled coil

200 – 245

Potential

Coiled coil

331 – 367

Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Experimental info

Sequence conflict

N → S in CAA81023. Ref.1

Sequence conflict

114

K → R in CAA81023. Ref.1

Sequence conflict

193

P → A in CAA81023. Ref.1

Sequence conflict

201

D → E in CAA81023. Ref.1

Sequence conflict

264

S → N in CAA81023. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P36224 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 80646B78B484AE56
Show »

FASTA

373

43,607

        10         20         30         40         50         60 
MNVIDLSDPA INVDYDSLIG IDNEESQEIF ENEVKEDGQQ EEQEEASSRK DGLIVEPGRD 

        70         80         90        100        110        120 
VESLRRAIRD QLLFKIHRQN QSDCADARKL SNDEEDESRQ QKLERIREEL EELKIENLTS 

       130        140        150        160        170        180 
EMQTEIKELC EIQSKLATES SSRLTNLRKK LLETYEGQDT VILPNIILDT SNIKRLQKLD 

       190        200        210        220        230        240 
QKISLMERFV GIPEALEAEE DRKSVHSKVN ELYRSIQLLQ GDDKAEGKLQ KFRDRLVELN 

       250        260        270        280        290        300 
EEFENSLLGK KIQQDLRLKD DTVSKLVMPE NKVKEINSMY SMFKQYQDSL PLLAERMKSL 

       310        320        330        340        350        360 
NKMNNRVIEV YETTKGLDSQ ITSIQEQGKV WLKALNELDK KFDEQEVKIR ENMEQIRRKI 

       370 
DTLEDEALQR NSK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle." McMillan J.N., Tatchell K. J. Cell Biol. 125:143-158(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The reference genome sequence of Saccharomyces cerevisiae: Then and now." Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M. G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B." Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A. Mol. Biol. Cell 9:1741-1756(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, FUNCTION OF THE DYNACTIN COMPLEX.
[5]	"Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae." Suzuki M., Igarashi R., Sekiya M., Utsugi T., Morishita S., Yukawa M., Ohya Y. Nat. Cell Biol. 6:861-871(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE DYNACTIN COMPLEX.
[6]	"Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued." Clark S.W., Rose M.D. Mol. Biol. Cell 16:3999-4012(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARP1.
[7]	"Arp10p is a pointed-end-associated component of yeast dynactin." Clark S.W., Rose M.D. Mol. Biol. Cell 17:738-748(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SELF-ASSOCIATION, INTERACTION WITH ARP1 AND ARP10.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25750 Genomic DNA. Translation: CAA81023.1. X80836 Genomic DNA. Translation: CAA56803.1. BK006946 Genomic DNA. Translation: DAA10195.1.
PIR	S47455.
RefSeq	NP_014022.1. NM_001182802.1.
3D structure databases
ProteinModelPortal	P36224.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35474. 287 interactions.
DIP	DIP-2387N.
IntAct	P36224. 9 interactions.
MINT	MINT-493774.
STRING	4932.YMR294W.
Proteomic databases
MaxQB	P36224.
PaxDb	P36224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR294W; YMR294W; YMR294W.
GeneID	855339.
KEGG	sce:YMR294W.
Organism-specific databases
CYGD	YMR294w.
SGD	S000004908. JNM1.
Phylogenomic databases
eggNOG	NOG285417.
OMA	DAGHCER.
OrthoDB	EOG7J189X.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32964-MONOMER.
Gene expression databases
Genevestigator	P36224.
Family and domain databases
InterPro	IPR028133. Dynamitin. [Graphical view]
PANTHER	PTHR15346:SF0. PTHR15346:SF0. 1 hit.
ProtoNet	Search...
Other
NextBio	979076.

Entry information

Entry name

JNM1_YEAST

Accession

Primary (citable) accession number: P36224
Secondary accession number(s): D6W0C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 1, 1996
Last modified:	June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents