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P36224

- JNM1_YEAST

UniProt

P36224 - JNM1_YEAST

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Protein

Nuclear migration protein JNM1

Gene

JNM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding (By similarity). The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. JNM1 is associated with the rod and links it to the projecting sidearm. Required for proper nuclear migration during the mitotic cell cycle and for astral microtubule development.By similarity2 Publications

GO - Molecular functioni

  1. motor activity Source: UniProtKB-KW
  2. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. establishment of mitotic spindle orientation Source: SGD
  2. mitotic nuclear division Source: InterPro
  3. nuclear migration Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-32964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear migration protein JNM1
Gene namesi
Name:JNM1
Synonyms:INS1
Ordered Locus Names:YMR294W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR294w.
SGDiS000004908. JNM1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. dynactin complex Source: SGD
  3. dynein complex Source: UniProtKB-KW
  4. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Nuclear migration protein JNM1PRO_0000084289Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei91 – 911Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36224.
PaxDbiP36224.

Expressioni

Gene expression databases

GenevestigatoriP36224.

Interactioni

Subunit structurei

Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of ARP1 polymers attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod structure is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding (By similarity). Interacts with ARP1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP1P386969EBI-9415,EBI-2920
ARP10Q045493EBI-9415,EBI-2977
LDB18Q078874EBI-9415,EBI-33243
NIP100P334203EBI-9415,EBI-12049

Protein-protein interaction databases

BioGridi35474. 290 interactions.
DIPiDIP-2387N.
IntActiP36224. 9 interactions.
MINTiMINT-493774.
STRINGi4932.YMR294W.

Structurei

3D structure databases

ProteinModelPortaliP36224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili114 – 13926Sequence AnalysisAdd
BLAST
Coiled coili200 – 24546Sequence AnalysisAdd
BLAST
Coiled coili331 – 36737Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG285417.
InParanoidiP36224.
OMAiDAGHCER.
OrthoDBiEOG7J189X.

Family and domain databases

InterProiIPR028133. Dynamitin.
[Graphical view]
PANTHERiPTHR15346:SF0. PTHR15346:SF0. 1 hit.

Sequencei

Sequence statusi: Complete.

P36224-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNVIDLSDPA INVDYDSLIG IDNEESQEIF ENEVKEDGQQ EEQEEASSRK
60 70 80 90 100
DGLIVEPGRD VESLRRAIRD QLLFKIHRQN QSDCADARKL SNDEEDESRQ
110 120 130 140 150
QKLERIREEL EELKIENLTS EMQTEIKELC EIQSKLATES SSRLTNLRKK
160 170 180 190 200
LLETYEGQDT VILPNIILDT SNIKRLQKLD QKISLMERFV GIPEALEAEE
210 220 230 240 250
DRKSVHSKVN ELYRSIQLLQ GDDKAEGKLQ KFRDRLVELN EEFENSLLGK
260 270 280 290 300
KIQQDLRLKD DTVSKLVMPE NKVKEINSMY SMFKQYQDSL PLLAERMKSL
310 320 330 340 350
NKMNNRVIEV YETTKGLDSQ ITSIQEQGKV WLKALNELDK KFDEQEVKIR
360 370
ENMEQIRRKI DTLEDEALQR NSK
Length:373
Mass (Da):43,607
Last modified:October 1, 1996 - v2
Checksum:i80646B78B484AE56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921N → S in CAA81023. (PubMed:8138567)Curated
Sequence conflicti114 – 1141K → R in CAA81023. (PubMed:8138567)Curated
Sequence conflicti193 – 1931P → A in CAA81023. (PubMed:8138567)Curated
Sequence conflicti201 – 2011D → E in CAA81023. (PubMed:8138567)Curated
Sequence conflicti264 – 2641S → N in CAA81023. (PubMed:8138567)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25750 Genomic DNA. Translation: CAA81023.1.
X80836 Genomic DNA. Translation: CAA56803.1.
BK006946 Genomic DNA. Translation: DAA10195.1.
PIRiS47455.
RefSeqiNP_014022.1. NM_001182802.1.

Genome annotation databases

EnsemblFungiiYMR294W; YMR294W; YMR294W.
GeneIDi855339.
KEGGisce:YMR294W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25750 Genomic DNA. Translation: CAA81023.1 .
X80836 Genomic DNA. Translation: CAA56803.1 .
BK006946 Genomic DNA. Translation: DAA10195.1 .
PIRi S47455.
RefSeqi NP_014022.1. NM_001182802.1.

3D structure databases

ProteinModelPortali P36224.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35474. 290 interactions.
DIPi DIP-2387N.
IntActi P36224. 9 interactions.
MINTi MINT-493774.
STRINGi 4932.YMR294W.

Proteomic databases

MaxQBi P36224.
PaxDbi P36224.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR294W ; YMR294W ; YMR294W .
GeneIDi 855339.
KEGGi sce:YMR294W.

Organism-specific databases

CYGDi YMR294w.
SGDi S000004908. JNM1.

Phylogenomic databases

eggNOGi NOG285417.
InParanoidi P36224.
OMAi DAGHCER.
OrthoDBi EOG7J189X.

Enzyme and pathway databases

BioCyci YEAST:G3O-32964-MONOMER.

Miscellaneous databases

NextBioi 979076.

Gene expression databases

Genevestigatori P36224.

Family and domain databases

InterProi IPR028133. Dynamitin.
[Graphical view ]
PANTHERi PTHR15346:SF0. PTHR15346:SF0. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle."
    McMillan J.N., Tatchell K.
    J. Cell Biol. 125:143-158(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B."
    Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A.
    Mol. Biol. Cell 9:1741-1756(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, FUNCTION OF THE DYNACTIN COMPLEX.
  5. "Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae."
    Suzuki M., Igarashi R., Sekiya M., Utsugi T., Morishita S., Yukawa M., Ohya Y.
    Nat. Cell Biol. 6:861-871(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE DYNACTIN COMPLEX.
  6. "Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued."
    Clark S.W., Rose M.D.
    Mol. Biol. Cell 16:3999-4012(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARP1.
  7. "Arp10p is a pointed-end-associated component of yeast dynactin."
    Clark S.W., Rose M.D.
    Mol. Biol. Cell 17:738-748(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SELF-ASSOCIATION, INTERACTION WITH ARP1 AND ARP10.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiJNM1_YEAST
AccessioniPrimary (citable) accession number: P36224
Secondary accession number(s): D6W0C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3