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Protein

Nuclear migration protein JNM1

Gene

JNM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding (By similarity). The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. JNM1 is associated with the rod and links it to the projecting sidearm. Required for proper nuclear migration during the mitotic cell cycle and for astral microtubule development.By similarity2 Publications

GO - Molecular functioni

  • motor activity Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • establishment of mitotic spindle orientation Source: SGD
  • nuclear migration Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-32964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear migration protein JNM1
Gene namesi
Name:JNM1
Synonyms:INS1
Ordered Locus Names:YMR294W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR294W.
SGDiS000004908. JNM1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842891 – 373Nuclear migration protein JNM1Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei91PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36224.
PRIDEiP36224.

PTM databases

iPTMnetiP36224.

Interactioni

Subunit structurei

Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of ARP1 polymers attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod structure is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding (By similarity). Interacts with ARP1.By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35474. 418 interactors.
DIPiDIP-2387N.
IntActiP36224. 7 interactors.
MINTiMINT-493774.
STRINGi4932.YMR294W.

Structurei

3D structure databases

ProteinModelPortaliP36224.
SMRiP36224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili114 – 139Sequence analysisAdd BLAST26
Coiled coili200 – 245Sequence analysisAdd BLAST46
Coiled coili331 – 367Sequence analysisAdd BLAST37

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiP36224.
KOiK18599.
OMAiDCERIVE.
OrthoDBiEOG092C3M4H.

Family and domain databases

InterProiView protein in InterPro
IPR028133. Dynamitin.
PANTHERiPTHR15346. PTHR15346. 2 hits.
PfamiView protein in Pfam
PF04912. Dynamitin. 1 hit.

Sequencei

Sequence statusi: Complete.

P36224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVIDLSDPA INVDYDSLIG IDNEESQEIF ENEVKEDGQQ EEQEEASSRK
60 70 80 90 100
DGLIVEPGRD VESLRRAIRD QLLFKIHRQN QSDCADARKL SNDEEDESRQ
110 120 130 140 150
QKLERIREEL EELKIENLTS EMQTEIKELC EIQSKLATES SSRLTNLRKK
160 170 180 190 200
LLETYEGQDT VILPNIILDT SNIKRLQKLD QKISLMERFV GIPEALEAEE
210 220 230 240 250
DRKSVHSKVN ELYRSIQLLQ GDDKAEGKLQ KFRDRLVELN EEFENSLLGK
260 270 280 290 300
KIQQDLRLKD DTVSKLVMPE NKVKEINSMY SMFKQYQDSL PLLAERMKSL
310 320 330 340 350
NKMNNRVIEV YETTKGLDSQ ITSIQEQGKV WLKALNELDK KFDEQEVKIR
360 370
ENMEQIRRKI DTLEDEALQR NSK
Length:373
Mass (Da):43,607
Last modified:October 1, 1996 - v2
Checksum:i80646B78B484AE56
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92N → S in CAA81023 (PubMed:8138567).Curated1
Sequence conflicti114K → R in CAA81023 (PubMed:8138567).Curated1
Sequence conflicti193P → A in CAA81023 (PubMed:8138567).Curated1
Sequence conflicti201D → E in CAA81023 (PubMed:8138567).Curated1
Sequence conflicti264S → N in CAA81023 (PubMed:8138567).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25750 Genomic DNA. Translation: CAA81023.1.
X80836 Genomic DNA. Translation: CAA56803.1.
BK006946 Genomic DNA. Translation: DAA10195.1.
PIRiS47455.
RefSeqiNP_014022.1. NM_001182802.1.

Genome annotation databases

EnsemblFungiiYMR294W; YMR294W; YMR294W.
GeneIDi855339.
KEGGisce:YMR294W.

Similar proteinsi

Entry informationi

Entry nameiJNM1_YEAST
AccessioniPrimary (citable) accession number: P36224
Secondary accession number(s): D6W0C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names