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P36224

- JNM1_YEAST

UniProt

P36224 - JNM1_YEAST

Protein

Nuclear migration protein JNM1

Gene

JNM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding By similarity. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. JNM1 is associated with the rod and links it to the projecting sidearm. Required for proper nuclear migration during the mitotic cell cycle and for astral microtubule development.By similarity2 Publications

    GO - Molecular functioni

    1. motor activity Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. establishment of mitotic spindle orientation Source: SGD
    2. mitotic nuclear division Source: InterPro
    3. nuclear migration Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32964-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear migration protein JNM1
    Gene namesi
    Name:JNM1
    Synonyms:INS1
    Ordered Locus Names:YMR294W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR294w.
    SGDiS000004908. JNM1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. dynactin complex Source: SGD
    3. dynein complex Source: UniProtKB-KW
    4. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Dynein, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Nuclear migration protein JNM1PRO_0000084289Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei91 – 911Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36224.
    PaxDbiP36224.

    Expressioni

    Gene expression databases

    GenevestigatoriP36224.

    Interactioni

    Subunit structurei

    Component of the dynactin complex composed of at least ARP1, JNM1, NIP100 and ARP10. Dynactin comprises a short rod of ARP1 polymers attached to ARP10 at its pointed-end and probably associated with the capping protein at its barbed-end. The rod structure is implicated in dynein cargo binding. A sidearm formed by NIP100 projects from the ARP1 filament and is implicated in motor binding By similarity. Interacts with ARP1.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARP1P386969EBI-9415,EBI-2920
    ARP10Q045493EBI-9415,EBI-2977
    LDB18Q078874EBI-9415,EBI-33243
    NIP100P334203EBI-9415,EBI-12049

    Protein-protein interaction databases

    BioGridi35474. 289 interactions.
    DIPiDIP-2387N.
    IntActiP36224. 9 interactions.
    MINTiMINT-493774.
    STRINGi4932.YMR294W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36224.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili114 – 13926Sequence AnalysisAdd
    BLAST
    Coiled coili200 – 24546Sequence AnalysisAdd
    BLAST
    Coiled coili331 – 36737Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG285417.
    OMAiDAGHCER.
    OrthoDBiEOG7J189X.

    Family and domain databases

    InterProiIPR028133. Dynamitin.
    [Graphical view]
    PANTHERiPTHR15346:SF0. PTHR15346:SF0. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36224-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNVIDLSDPA INVDYDSLIG IDNEESQEIF ENEVKEDGQQ EEQEEASSRK    50
    DGLIVEPGRD VESLRRAIRD QLLFKIHRQN QSDCADARKL SNDEEDESRQ 100
    QKLERIREEL EELKIENLTS EMQTEIKELC EIQSKLATES SSRLTNLRKK 150
    LLETYEGQDT VILPNIILDT SNIKRLQKLD QKISLMERFV GIPEALEAEE 200
    DRKSVHSKVN ELYRSIQLLQ GDDKAEGKLQ KFRDRLVELN EEFENSLLGK 250
    KIQQDLRLKD DTVSKLVMPE NKVKEINSMY SMFKQYQDSL PLLAERMKSL 300
    NKMNNRVIEV YETTKGLDSQ ITSIQEQGKV WLKALNELDK KFDEQEVKIR 350
    ENMEQIRRKI DTLEDEALQR NSK 373
    Length:373
    Mass (Da):43,607
    Last modified:October 1, 1996 - v2
    Checksum:i80646B78B484AE56
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti92 – 921N → S in CAA81023. (PubMed:8138567)Curated
    Sequence conflicti114 – 1141K → R in CAA81023. (PubMed:8138567)Curated
    Sequence conflicti193 – 1931P → A in CAA81023. (PubMed:8138567)Curated
    Sequence conflicti201 – 2011D → E in CAA81023. (PubMed:8138567)Curated
    Sequence conflicti264 – 2641S → N in CAA81023. (PubMed:8138567)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z25750 Genomic DNA. Translation: CAA81023.1.
    X80836 Genomic DNA. Translation: CAA56803.1.
    BK006946 Genomic DNA. Translation: DAA10195.1.
    PIRiS47455.
    RefSeqiNP_014022.1. NM_001182802.1.

    Genome annotation databases

    EnsemblFungiiYMR294W; YMR294W; YMR294W.
    GeneIDi855339.
    KEGGisce:YMR294W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z25750 Genomic DNA. Translation: CAA81023.1 .
    X80836 Genomic DNA. Translation: CAA56803.1 .
    BK006946 Genomic DNA. Translation: DAA10195.1 .
    PIRi S47455.
    RefSeqi NP_014022.1. NM_001182802.1.

    3D structure databases

    ProteinModelPortali P36224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35474. 289 interactions.
    DIPi DIP-2387N.
    IntActi P36224. 9 interactions.
    MINTi MINT-493774.
    STRINGi 4932.YMR294W.

    Proteomic databases

    MaxQBi P36224.
    PaxDbi P36224.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR294W ; YMR294W ; YMR294W .
    GeneIDi 855339.
    KEGGi sce:YMR294W.

    Organism-specific databases

    CYGDi YMR294w.
    SGDi S000004908. JNM1.

    Phylogenomic databases

    eggNOGi NOG285417.
    OMAi DAGHCER.
    OrthoDBi EOG7J189X.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32964-MONOMER.

    Miscellaneous databases

    NextBioi 979076.

    Gene expression databases

    Genevestigatori P36224.

    Family and domain databases

    InterProi IPR028133. Dynamitin.
    [Graphical view ]
    PANTHERi PTHR15346:SF0. PTHR15346:SF0. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle."
      McMillan J.N., Tatchell K.
      J. Cell Biol. 125:143-158(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B."
      Kahana J.A., Schlenstedt G., Evanchuk D.M., Geiser J.R., Hoyt M.A., Silver P.A.
      Mol. Biol. Cell 9:1741-1756(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE DYNACTIN COMPLEX, FUNCTION OF THE DYNACTIN COMPLEX.
    5. "Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae."
      Suzuki M., Igarashi R., Sekiya M., Utsugi T., Morishita S., Yukawa M., Ohya Y.
      Nat. Cell Biol. 6:861-871(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE DYNACTIN COMPLEX.
    6. "Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued."
      Clark S.W., Rose M.D.
      Mol. Biol. Cell 16:3999-4012(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARP1.
    7. "Arp10p is a pointed-end-associated component of yeast dynactin."
      Clark S.W., Rose M.D.
      Mol. Biol. Cell 17:738-748(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SELF-ASSOCIATION, INTERACTION WITH ARP1 AND ARP10.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiJNM1_YEAST
    AccessioniPrimary (citable) accession number: P36224
    Secondary accession number(s): D6W0C1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3