P36223 (GLND_AZOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Protein-PII] uridylyltransferase
Short name=PII uridylyl-transferase
EC=2.7.7.59

Alternative name(s):
UTase
Uridylyl-removing enzyme

Gene names

Name:	glnD
Synonyms:	nfrX

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter group › Azotobacter

Protein attributes

Sequence length	899 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (GlnB) regulatory protein. HAMAP-Rule MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277
Sequence similarities	Belongs to the GlnD family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 899	899	[Protein-PII] uridylyltransferase HAMAP-Rule MF_00277		PRO_0000192717

Sequences

	Sequence		Length	Mass (Da)
	P36223 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 96AF63BC5EDE3C9D	FASTA	899	102,580
	10 20 30 40 50 60 MPQVDPDLFD PGQFQAELAL KSSPIPAYKK ALRCAREVLD ARFQEGRDIR RLIEDRAWFV 70 80 90 100 110 120 DQILALAWNR FDWSEDADIA LIAVGGYGRG ELHPYSDIDL LILMDGADHE VFREPIEGFL 130 140 150 160 170 180 TLLWDIGLEV GQSVRSLAEC AEEAQADLTV ITNLMESRTI AGPEHLRQRM QEVTSAQRMW 190 200 210 220 230 240 PSRAFFLAKR DEQKTRHARY NDTEYNLEPN VKGSPGGLRD IQTLLWIARR QFGTINLHAM 250 260 270 280 290 300 VGQGFLLESE YTLLASSQEF LWKVRYALHM LAGRAEDRLL FDLQRQIAGL LGYEDSDAKL 310 320 330 340 350 360 AVERFMQKYY RVVLGIAELT ELVFQHFEEV ILPGDAAGRV EPLNERFQVR DGYLEVTHAG 370 380 390 400 410 420 VFQETPSALL EIFVLLARRP EIRGVRADTI RLLRDHRYLI DDAFRRDPHN TGLFIELFKS 430 440 450 460 470 480 RQGIHRNLRR MNRYGILGRY LPEFGHIVGQ MQHDLFHIYT VDAHTLNLIK NLRKLFWPEL 490 500 510 520 530 540 AEKYPLASKL IEKLPKPELI YLAGLYHDIG KGRGGDHSEL GAADALAFCQ RHDLPAMDTQ 550 560 570 580 590 600 LIVWLVRNHL LMSTTAQRKD LSDPQVIFDF AQKVRDQTYL DYLYVLTVAD INATNPTLWN 610 620 630 640 650 660 SWRASLLRQL YTETKHALRR GLEQPVGREE QIRQTQKAAL DILVRSGTDP DDAEHLWTQL 670 680 690 700 710 720 GDDYFLRHTS SDIAWHTEAI LQHPSSGGPL VLIKETTQRE FEGATQIFIY APDQHDFFAV 730 740 750 760 770 780 TVAAMDQLNL SIHDARVITS TSQFTLDTYI VLDADGGSIG NNPARIQEIR QGLVEALRNP 790 800 810 820 830 840 ADYPTIIQRR VPRQLKHFAF APQVTIQNDA LRPVTILEII APDRPGLLAR IGKIFLDFDL 850 860 870 880 890 SLQNAKIATL GERVEDVFFV TDAHNQPLSD PELCARLQLA IAEQLADGDS YIQPSRISI

References

[1]

"The product of the nitrogen fixation regulatory gene nfrX of Azotobacter vinelandii is functionally and structurally homologous to the uridylyltransferase encoded by glnD in enteric bacteria." Contreras A., Drummond M., Bali A., Blanco G., Garcia E., Bush G., Kennedy C., Merrick M. J. Bacteriol. 173:7741-7749(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59610 Genomic DNA. Translation: CAA42173.1.
PIR	S24223.
3D structure databases
ProteinModelPortal	P36223.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_00277. PII_uridylyl-transf.
InterPro	IPR002912. ACT_dom. IPR010043. GlnD_Uridyltrans. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. PTHR13734:SF1. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_AZOVI

Accession

Primary (citable) accession number: P36223

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program