SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P36216

- MTC1_PBCVI

UniProt

P36216 - MTC1_PBCVI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Modification methylase CviJI
Gene
CVIJIM
Organism
Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence RGCY, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the CviJI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3353. M.CviJI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase CviJI (EC:2.1.1.37)
Short name:
M.CviJI
Alternative name(s):
Cytosine-specific methyltransferase CviJI
Gene namesi
Name:CVIJIM
OrganismiParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Taxonomic identifieri46019 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 3071]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Modification methylase CviJI
PRO_0000087871Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP36216.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 367365SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36216-1 [UniParc]FASTAAdd to Basket

« Hide

MSFRTLELFA GIAGISHGLR GISTPVAFVE INEDAQKFLK TKFSDASVFN    50
DVTKFTKSDF PEDIDMITAG FPCTGFSIAG SRTGFEHKES GLFADVVRIT 100
EEYKPKIVFL ENSHMLSHTY NLDVVVKKMD EIGYFCKWVT CRASIIGAHH 150
QRHRWFCLAI RKDYEPEEII VSVNATKFDW ENNEPPCQVD NKSYENSTLV 200
RLAGYSVVPD QIRYAFTGLF TGDFESSWKT TLTPGTIIGT EHKKMKGTYD 250
KVINGYYEND VYYSFSRKEV HRAPLNISVK PRDIPEKHNG KTLVDREMIK 300
KYWCTPCASY GTATAGCNVL TDRQSHALPT QVRFSYRGVC GRHLSGIWCA 350
WLMGYDQEYL GYLVQYD 367
Length:367
Mass (Da):41,861
Last modified:June 1, 1994 - v1
Checksum:i15BD56B0354E2ECC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27265 Genomic DNA. Translation: AAA88826.1.
U09001 Genomic DNA. Translation: AAC55063.1.
PIRiA46355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27265 Genomic DNA. Translation: AAA88826.1 .
U09001 Genomic DNA. Translation: AAC55063.1 .
PIRi A46355.

3D structure databases

ProteinModelPortali P36216.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3353. M.CviJI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing the cytosine methyltransferase gene M. CviJI from Chlorella virus IL-3A."
    Shields S.L., Burbank D.E., Grabherr R., van Etten J.L.
    Virology 176:16-24(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A."
    Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M.
    Nucleic Acids Res. 24:2463-2469(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-367.

Entry informationi

Entry nameiMTC1_PBCVI
AccessioniPrimary (citable) accession number: P36216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi