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P36216 (MTC1_PBCVI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase CviJI

Short name=M.CviJI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase CviJI
Gene names
Name:CVIJIM
OrganismParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Taxonomic identifier46019 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostChlorella [TaxID: 3071]

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence RGCY, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the CviJI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Modification methylase CviJI
PRO_0000087871

Regions

Domain3 – 367365SAM-dependent MTase C5-type

Sites

Active site731 By similarity

Sequences

Sequence LengthMass (Da)Tools
P36216 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 15BD56B0354E2ECC

FASTA36741,861
        10         20         30         40         50         60 
MSFRTLELFA GIAGISHGLR GISTPVAFVE INEDAQKFLK TKFSDASVFN DVTKFTKSDF 

        70         80         90        100        110        120 
PEDIDMITAG FPCTGFSIAG SRTGFEHKES GLFADVVRIT EEYKPKIVFL ENSHMLSHTY 

       130        140        150        160        170        180 
NLDVVVKKMD EIGYFCKWVT CRASIIGAHH QRHRWFCLAI RKDYEPEEII VSVNATKFDW 

       190        200        210        220        230        240 
ENNEPPCQVD NKSYENSTLV RLAGYSVVPD QIRYAFTGLF TGDFESSWKT TLTPGTIIGT 

       250        260        270        280        290        300 
EHKKMKGTYD KVINGYYEND VYYSFSRKEV HRAPLNISVK PRDIPEKHNG KTLVDREMIK 

       310        320        330        340        350        360 
KYWCTPCASY GTATAGCNVL TDRQSHALPT QVRFSYRGVC GRHLSGIWCA WLMGYDQEYL 


GYLVQYD 

« Hide

References

[1]"Cloning and sequencing the cytosine methyltransferase gene M. CviJI from Chlorella virus IL-3A."
Shields S.L., Burbank D.E., Grabherr R., van Etten J.L.
Virology 176:16-24(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A."
Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M.
Nucleic Acids Res. 24:2463-2469(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-367.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27265 Genomic DNA. Translation: AAA88826.1.
U09001 Genomic DNA. Translation: AAC55063.1.
PIRA46355.

3D structure databases

ProteinModelPortalP36216.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3353. M.CviJI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTC1_PBCVI
AccessionPrimary (citable) accession number: P36216
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries