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P36216

- MTC1_PBCVI

UniProt

P36216 - MTC1_PBCVI

Protein

Modification methylase CviJI

Gene

CVIJIM

Organism
Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence RGCY, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the CviJI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3353. M.CviJI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase CviJI (EC:2.1.1.37)
    Short name:
    M.CviJI
    Alternative name(s):
    Cytosine-specific methyltransferase CviJI
    Gene namesi
    Name:CVIJIM
    OrganismiParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
    Taxonomic identifieri46019 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
    Virus hostiChlorella [TaxID: 3071]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Modification methylase CviJIPRO_0000087871Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP36216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 367365SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36216-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFRTLELFA GIAGISHGLR GISTPVAFVE INEDAQKFLK TKFSDASVFN    50
    DVTKFTKSDF PEDIDMITAG FPCTGFSIAG SRTGFEHKES GLFADVVRIT 100
    EEYKPKIVFL ENSHMLSHTY NLDVVVKKMD EIGYFCKWVT CRASIIGAHH 150
    QRHRWFCLAI RKDYEPEEII VSVNATKFDW ENNEPPCQVD NKSYENSTLV 200
    RLAGYSVVPD QIRYAFTGLF TGDFESSWKT TLTPGTIIGT EHKKMKGTYD 250
    KVINGYYEND VYYSFSRKEV HRAPLNISVK PRDIPEKHNG KTLVDREMIK 300
    KYWCTPCASY GTATAGCNVL TDRQSHALPT QVRFSYRGVC GRHLSGIWCA 350
    WLMGYDQEYL GYLVQYD 367
    Length:367
    Mass (Da):41,861
    Last modified:June 1, 1994 - v1
    Checksum:i15BD56B0354E2ECC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27265 Genomic DNA. Translation: AAA88826.1.
    U09001 Genomic DNA. Translation: AAC55063.1.
    PIRiA46355.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27265 Genomic DNA. Translation: AAA88826.1 .
    U09001 Genomic DNA. Translation: AAC55063.1 .
    PIRi A46355.

    3D structure databases

    ProteinModelPortali P36216.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3353. M.CviJI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing the cytosine methyltransferase gene M. CviJI from Chlorella virus IL-3A."
      Shields S.L., Burbank D.E., Grabherr R., van Etten J.L.
      Virology 176:16-24(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A."
      Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M.
      Nucleic Acids Res. 24:2463-2469(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 357-367.

    Entry informationi

    Entry nameiMTC1_PBCVI
    AccessioniPrimary (citable) accession number: P36216
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3