Reviewed, UniProtKB/Swiss-Prot P36209 (STS1_SCHPO)

Names and origin

Protein names

Recommended name:
    Delta(24(24(1)))-sterol reductase
    EC=1.3.1.71
Alternative name(s):
    Sterol Delta(24(28))-reductase
    C-24(28) sterol reductase

Gene names

Name:	sts1
ORF Names:	SPAC20G4.07c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Ergosterol + NADP+ = ergosta-5,7,22,24(241)-tetraen-3-beta-ol + NADPH.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cation stress Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe response to drug Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	delta24(24-1) sterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Delta(24(24(1)))-sterol reductase		PRO_0000207492
Regions
	Transmembrane	13 – 33	21	Potential
	Transmembrane	77 – 97	21	Potential
	Transmembrane	118 – 138	21	Potential
	Transmembrane	153 – 173	21	Potential
	Transmembrane	209 – 229	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	311 – 331	21	Potential
	Transmembrane	399 – 419	21	Potential
Experimental info
	Sequence conflict	412	1	C → S in CAA45113. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36209-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 4740B6EE3BBD27CF	FASTA	453	52,546
	10 20 30 40 50 60 MKSTVKKSAP REFGGAKGAL AIMTGFPCLM YYLWACSKFN DSQFIKPESF TIAGFQNFFR 70 80 90 100 110 120 TLGHYIYVGA YPTRYAFLVF WSFCIVQAVM YLTLPGVRTQ GLPLKHRNNE RLPYLCNAIW 130 140 150 160 170 180 SFYTTIVILA VLHVTHVFPI TTFIDMFGPL MSVAIITAFV CTFVLYTGTL LFGDRLFDKP 190 200 210 220 230 240 HRLSGNPIYD AFMGACLNPR LGKLLDFKMF FEVRIPWFIL FFISVGAAVR QYETYGTVSP 250 260 270 280 290 300 QVLFVCLGHY LYANACSKGE QLIVPTWDMA YEKFGFMLIF WNMAGVPFTY SHCTLYLFSH 310 320 330 340 350 360 DPSVYNWSTQ YTTGIYVLLL CCYYIFDTCN GQKNHFRNQI YGTEVHRKTF PQLPWLIIKN 370 380 390 400 410 420 PTFIRCANGG TLLTSGWYRY ARKIHYTADF FQSLSWALIT GFQSPLPYFY PCFFFVVLVH 430 440 450 RVSRDIKKCK AKYGADFDEY CRICPYLFIP YIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fission yeast sts1+ gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation." Shimanuki M., Goebl M., Yanagida M., Toda T. Mol. Biol. Cell 3:263-273(1992) [PubMed: 1320960] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway." Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirsch D.R. Gene 140:41-49(1994) [PubMed: 8125337] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63549 Genomic DNA. Translation: CAA45113.1. CU329670 Genomic DNA. Translation: CAB11256.1.
PIR	A43765.
RefSeq	NP_594742.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P36209.
Genome annotation databases
GeneID	2541740.
GenomeReviews	Gene locus sts1 in contig CU329670_GR.
KEGG	spo:SPAC20G4.07c.
NMPDR	fig|4896.1.peg.4712.
Organism-specific databases
GeneDB_Spombe	SPAC20G4.07c.
Phylogenomic databases
eggNOG	fuNOG07477.
HOGENOM	HBG592488.
OMA	DMAYEKF.
OrthoDB	EOG9XPRZ1.
PhylomeDB	P36209.
Enzyme and pathway databases
BRENDA	1.3.1.71. 653.
Gene expression databases
ArrayExpress	P36209.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STS1_SCHPO

Accession

Primary (citable) accession number: P36209
Secondary accession number(s): O13891

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	July 15, 1998
Last modified:	January 19, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)