Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine synthetase

Gene

glnA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei59Important for inhibition by glutamineBy similarity1
Metal bindingi128Magnesium 1By similarity1
Metal bindingi130Magnesium 2By similarity1
Binding sitei180ATPBy similarity1
Metal bindingi185Magnesium 2By similarity1
Metal bindingi192Magnesium 2By similarity1
Binding sitei237L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi241Magnesium 1; via pros nitrogenBy similarity1
Binding sitei245ATPBy similarity1
Binding sitei294L-glutamateBy similarity1
Binding sitei300L-glutamateBy similarity1
Binding sitei312ATPBy similarity1
Binding sitei312L-glutamateBy similarity1
Binding sitei317ATPBy similarity1
Binding sitei324ATPBy similarity1
Metal bindingi329Magnesium 1By similarity1
Binding sitei331L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi243 – 245ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-502.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnA1 Publication
Ordered Locus Names:TM_0943
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532731 – 439Glutamine synthetaseAdd BLAST439

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Protein-protein interaction databases

STRINGi243274.TM0943.

Structurei

3D structure databases

ProteinModelPortaliP36205.
SMRiP36205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 237L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
InParanoidiP36205.
KOiK01915.
OMAiTFYTHPE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P36205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIETIKRII EEENVRFIRL QFTDINGTLK NLEITPDVFL ESWEDGIMFD
60 70 80 90 100
GSSIEGFVRI EESDMYLKPV LDTFAVLPWT VDGAKSARVI CDVYTPDGKP
110 120 130 140 150
FEGDPRYRLR RMMEKAEQLG YTPYAGPEME FFILPINEKG EPVPEFLDHG
160 170 180 190 200
GYFDLLPLSK VEEIRRDIAI ALEKMGITVE ATHHEVAPSQ HEVDFRYDTF
210 220 230 240 250
LRTADNAQTV KLVIKTMAIF HGYHATFMPK PFYGVNGSGM HVHMSLFRGD
260 270 280 290 300
KNAFYDPDDP LGLSKELRYF VGGILKHAKA LAAVTNPTIN SYKRLVPGYE
310 320 330 340 350
APVYISWSVG NRSALIRIPK ARGKATRLEY RSPDPSCNIY LAFAAILAAG
360 370 380 390 400
LDGIINKIEP PAPVEENIYH MTSERREELN IESLPGSLKE AVEELKKDDV
410 420 430
IIDALGEHIF EKFVEAAEKD WKEFSTYVTN WELQRYLYL
Length:439
Mass (Da):50,035
Last modified:May 30, 2000 - v2
Checksum:iABE3E674BD2F2359
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204A → G in CAA42729 (PubMed:1348781).Curated1
Sequence conflicti280A → R in CAA42729 (PubMed:1348781).Curated1
Sequence conflicti336S → T in CAA42729 (PubMed:1348781).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60160 Genomic DNA. Translation: CAA42729.1.
AE000512 Genomic DNA. Translation: AAD36024.1.
PIRiB72313.
RefSeqiNP_228751.1. NC_000853.1.
WP_004080619.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36024; AAD36024; TM_0943.
GeneIDi898680.
KEGGitma:TM0943.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60160 Genomic DNA. Translation: CAA42729.1.
AE000512 Genomic DNA. Translation: AAD36024.1.
PIRiB72313.
RefSeqiNP_228751.1. NC_000853.1.
WP_004080619.1. NZ_CP011107.1.

3D structure databases

ProteinModelPortaliP36205.
SMRiP36205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36024; AAD36024; TM_0943.
GeneIDi898680.
KEGGitma:TM0943.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
InParanoidiP36205.
KOiK01915.
OMAiTFYTHPE.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-502.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1A_THEMA
AccessioniPrimary (citable) accession number: P36205
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 30, 2000
Last modified: June 7, 2017
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.