Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P36205 (GLNA_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

Short name=GS
EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:TM_0943
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Glutamine synthetase
PRO_0000153273

Amino acid modifications

Modified residue3691O-AMP-tyrosine By similarity

Experimental info

Sequence conflict2041A → G in CAA42729. Ref.1
Sequence conflict2801A → R in CAA42729. Ref.1
Sequence conflict3361S → T in CAA42729. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P36205 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: ABE3E674BD2F2359

FASTA43950,035
        10         20         30         40         50         60 
MTIETIKRII EEENVRFIRL QFTDINGTLK NLEITPDVFL ESWEDGIMFD GSSIEGFVRI 

        70         80         90        100        110        120 
EESDMYLKPV LDTFAVLPWT VDGAKSARVI CDVYTPDGKP FEGDPRYRLR RMMEKAEQLG 

       130        140        150        160        170        180 
YTPYAGPEME FFILPINEKG EPVPEFLDHG GYFDLLPLSK VEEIRRDIAI ALEKMGITVE 

       190        200        210        220        230        240 
ATHHEVAPSQ HEVDFRYDTF LRTADNAQTV KLVIKTMAIF HGYHATFMPK PFYGVNGSGM 

       250        260        270        280        290        300 
HVHMSLFRGD KNAFYDPDDP LGLSKELRYF VGGILKHAKA LAAVTNPTIN SYKRLVPGYE 

       310        320        330        340        350        360 
APVYISWSVG NRSALIRIPK ARGKATRLEY RSPDPSCNIY LAFAAILAAG LDGIINKIEP 

       370        380        390        400        410        420 
PAPVEENIYH MTSERREELN IESLPGSLKE AVEELKKDDV IIDALGEHIF EKFVEAAEKD 

       430 
WKEFSTYVTN WELQRYLYL 

« Hide

References

« Hide 'large scale' references
[1]"The glnA gene of the extremely thermophilic eubacterium Thermotoga maritima: cloning, primary structure, and expression in Escherichia coli."
Sanangelantoni A.M., Forlani G., Ambroselli F., Cammarano P., Tiboni O.
J. Gen. Microbiol. 138:383-393(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60160 Genomic DNA. Translation: CAA42729.1.
AE000512 Genomic DNA. Translation: AAD36024.1.
PIRB72313.
RefSeqNP_228751.1. NC_000853.1.
YP_007977295.1. NC_021214.1.
YP_008991957.1. NC_023151.1.

3D structure databases

ProteinModelPortalP36205.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0943.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36024; AAD36024; TM_0943.
GeneID898680.
KEGGtma:TM0943.
tmi:THEMA_09630.
tmm:Tmari_0945.
PATRIC23936817. VBITheMar51294_0957.

Phylogenomic databases

eggNOGCOG0174.
KOK01915.
OMAIEWDMFR.
OrthoDBEOG6B360N.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-502.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_THEMA
AccessionPrimary (citable) accession number: P36205
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families