P36196 (ACES_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylcholinesterase
Short name=AChE
EC=3.1.1.7

Gene names

Name:

ACHE

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Catalytic activity	Acetylcholine + H2O = choline + acetate.
Subunit structure	Oligomer composed of disulfide-linked homodimers.
Subcellular location	Cell junction › synapse. Secreted. Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Secreted Synapse
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	choline metabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of axonogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of dendrite morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome synapse assembly Inferred from Biological aspect of Ancestor. Source: RefGenome synaptic transmission, cholinergic Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	axon Inferred from Biological aspect of Ancestor. Source: RefGenome cell junction Inferred from electronic annotation. Source: UniProtKB-KW cell surface Inferred from Biological aspect of Ancestor. Source: RefGenome dendrite Inferred from Biological aspect of Ancestor. Source: RefGenome endoplasmic reticulum lumen Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome neuromuscular junction Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from Biological aspect of Ancestor. Source: RefGenome presynaptic membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	acetylcholinesterase activity Inferred from Biological aspect of Ancestor. Source: RefGenome carboxylesterase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 767	748	Acetylcholinesterase		PRO_0000008591
Sites
	Active site	227	1	Acyl-ester intermediate By similarity
	Active site	520	1	Charge relay system By similarity
	Active site	633	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Glycosylation	536	1	N-linked (GlcNAc...) Potential
	Glycosylation	650	1	N-linked (GlcNAc...) Potential
	Glycosylation	725	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 121		By similarity
	Disulfide bond	281 ↔ 292		By similarity
	Disulfide bond	595 ↔ 713		By similarity
	Disulfide bond	764		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36196 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B1B3DF29C31F6062	FASTA	767	83,020
	10 20 30 40 50 60 MAPLFLLLLL LLSPSPTSAH RFAYSAPNRP EVRTTTGSVR GLLIPAGPSG STAAAFLGIP 70 80 90 100 110 120 FAVPPLGPLR FRPPLPIPTP WTGIRDADSQ PFACYQMVDT TFPGFQGSEM WNPNREMSED 130 140 150 160 170 180 CLYLNVWTQK GDPTEPPVLV WIYGGGFTGG SVSLDVYDGR YLAAAEEAVV VSMNYRVGSL 190 200 210 220 230 240 GFLALAGHRD APGNVGLWDQ RLALQWVRDN AEAFGGDPDL ITLFGESAGA ASVGFHLLSP 250 260 270 280 290 300 HSKGLFRRAV LQSGSPNGPW ATIGAAEGRR RAAALGRAVG CPYGNETEFL GCLRGKEAAD 310 320 330 340 350 360 VLEGEGVVMP PQSVFRFAFV PVVDGDFVVD SPDVALWGDY GVKGGEGGHG VEGGDGGYGV 370 380 390 400 410 420 KGGDGVKGGY GGGYGARGVR EGDGDGGYGV KEGLREGYGV KEGYGVEGDG ANAYGARVPP 430 440 450 460 470 480 RPHRDETPPD AYGAKGSADA YGAKAAPRPH RDETSPDAYG AKMPPRPHRD EASPDTYGAK 490 500 510 520 530 540 MPPRPHRDET SPDAYGAKMP PRPHRAGGEV EVLLGAVRVE GSYFLVYGVP GFGKDNESLI 550 560 570 580 590 600 SREEFLGGVR MGVPQATELA AEAVVLHYTD WLDADNPVKN REALDDIVGD HNVVCPLMAF 610 620 630 640 650 660 AQRWAQRGGK VYAYLFDHRS STLLWPSWMG VPHGYEIEFV FGLPLEPRNN YTREEVELSR 670 680 690 700 710 720 RIMRYWGNFA RTGDPNGGVG GPRWPPYTPS GQRYAHLNAR PLSVGHGLRT QICAFWTRFL 730 740 750 760 PKLLNATGPP EDAEREWRLE FHRWSSYMGR WRTQFEHYSR QQPCATL

References

[1]	"Cloning and analysis of chicken acetylcholinesterase transcripts from muscle and brain." Randall W.R., Rimer M., Gough N.R. Biochim. Biophys. Acta 1218:453-456(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03472 mRNA. Translation: AAA60456.1.
IPI	IPI00575147.
PIR	S47639.
RefSeq	NP_990749.1. NM_205418.1.
UniGene	Gga.52373. Gga.793.
3D structure databases
ProteinModelPortal	P36196.
SMR	P36196. Positions 32-333, 510-727, 731-761.
ModBase	Search...
Protein family/group databases
MEROPS	S09.979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396388.
KEGG	gga:396388.
Organism-specific databases
CTD	43.
Phylogenomic databases
HOVERGEN	HBG008839.
KO	K01049.
Family and domain databases
InterPro	IPR014788. AChE_tetra. IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
Pfam	PF08674. AChE_tetra. 1 hit. PF00135. COesterase. 2 hits. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
ProDom	PD415333. AChE_tetra. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816430.

Entry information

Entry name

ACES_CHICK

Accession

Primary (citable) accession number: P36196

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program