P36195 (TDT_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA nucleotidylexotransferase
EC=2.7.7.31

Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name=Terminal transferase

Gene names

Name:	DNTT
Synonyms:	TDT

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Cofactor	Magnesium.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the DNA polymerase type-X family. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	Terminal addition
Cellular component	Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA modification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA nucleotidylexotransferase activity Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	DNA nucleotidylexotransferase		PRO_0000218795
Regions
	Domain	27 – 124	98	BRCT
	Region	336 – 345	10	Involved in ssDNA binding By similarity
Sites
	Metal binding	253	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	255	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	343	1	Magnesium By similarity
	Metal binding	345	1	Magnesium By similarity
	Metal binding	430	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36195 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 92B2BFD87B30E94E	FASTA	506	58,447
	10 20 30 40 50 60 MERIRPPTVV SQRKRQKGMY SPKLSCGYEI KFNKLVIFIM QRKMGMTRRT FLMELARSKG 70 80 90 100 110 120 FRVESELSDS VTHIVAENNS YPEVLDWLKG QAVGDSSRFE ILDISWLTAC MEMGRPVDLE 130 140 150 160 170 180 KKYHLVEQAG QYPTLKTPES EVSSFTASKV SQYSCQRKTT LNNCNKKFTD AFEIMAENYE 190 200 210 220 230 240 FKENEIFCLE FLRAASVLKS LPFPVTRMKD IQGLPCMGDR VRDVIEEIIE EGESSRAKDV 250 260 270 280 290 300 LNDERYKSFK EFTSVFGVGV KTSEKWFRMG LRTVEEVKAD KTLKLSKMQR AGFLYYEDLV 310 320 330 340 350 360 SCVSKAEADA VSSIVKNTVC TFLPDALVTI TGGFRRGKKI GHDIDFLITS PGQREDDELL 370 380 390 400 410 420 HKGLLLYCDI IESTFVKEQI PSRHVDAMDH FQKCFAILKL YQPRVDNSSY NMSKKCDMAE 430 440 450 460 470 480 VKDWKAIRVD LVITPFEQYA YALLGWTGSR QFGRDLRRYA THERKMMLDN HALYDKRKRV 490 500 FLKAGSEEEI FAHLGLDYVE PWERNA

References

[1]	"T-cell specific avian TdT: characterization of the cDNA and recombinant enzyme." Yang B., Gathy K.N., Coleman M.S. Nucleic Acids Res. 23:2041-2048(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06938 mRNA. Translation: AAA75280.1.
IPI	IPI00584380.
PIR	S55786.
RefSeq	NP_990720.1. NM_205389.1.
UniGene	Gga.774.
3D structure databases
ProteinModelPortal	P36195.
SMR	P36195. Positions 29-125, 149-506.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000032645.
Proteomic databases
PaxDb	P36195.
PRIDE	P36195.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396351.
KEGG	gga:396351.
Organism-specific databases
CTD	1791.
Phylogenomic databases
eggNOG	COG1796.
HOGENOM	HOG000263600.
HOVERGEN	HBG003670.
InParanoid	P36195.
KO	K00977.
OrthoDB	EOG4SQWWN.
Family and domain databases
InterPro	IPR001357. BRCT_dom. IPR002054. DNA-dir_DNA_pol_X. IPR019843. DNA_pol-X_BS. IPR010996. DNA_pol_b-like_N. IPR018944. DNA_pol_lambd_fingers_domain. IPR022312. DNA_pol_X. IPR002934. Nucleotidyltransferase. IPR027292. TdT. IPR001726. TdT/Mu. [Graphical view]
PANTHER	PTHR11276:SF6. PTHR11276:SF6. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF10391. DNA_pol_lambd_f. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF000817. DNA_NT. 1 hit. PIRSF501175. TDT. 1 hit.
PRINTS	PR00869. DNAPOLX. PR00871. DNAPOLXTDT.
SMART	SM00292. BRCT. 1 hit. SM00483. POLXc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF81585. DNA_pol_lambd_fingers_domain. 1 hit. SSF47802. DNApol_B_N_like. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS00522. DNA_POLYMERASE_X. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816398.

Entry information

Entry name

TDT_CHICK

Accession

Primary (citable) accession number: P36195

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program