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Protein

DNA nucleotidylexotransferase

Gene

DNTT

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

Mg2+By similarityNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi343 – 3431MagnesiumBy similarity
Metal bindingi345 – 3451MagnesiumBy similarity
Metal bindingi430 – 4301MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Terminal addition

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.31)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
Terminal transferase
Gene namesi
Name:DNTT
Synonyms:TDT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506DNA nucleotidylexotransferasePRO_0000218795Add
BLAST

Proteomic databases

PaxDbiP36195.
PRIDEiP36195.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000032645.

Structurei

3D structure databases

ProteinModelPortaliP36195.
SMRiP36195. Positions 29-125, 149-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 12498BRCTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni258 – 2625Involved in DNA bindingBy similarity
Regioni333 – 3386Deoxynucleoside triphosphate bindingBy similarity
Regioni342 – 3454Deoxynucleoside triphosphate bindingBy similarity
Regioni445 – 4462Deoxynucleoside triphosphate bindingBy similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiP36195.
KOiK00977.
PhylomeDBiP36195.

Family and domain databases

Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIRPPTVV SQRKRQKGMY SPKLSCGYEI KFNKLVIFIM QRKMGMTRRT
60 70 80 90 100
FLMELARSKG FRVESELSDS VTHIVAENNS YPEVLDWLKG QAVGDSSRFE
110 120 130 140 150
ILDISWLTAC MEMGRPVDLE KKYHLVEQAG QYPTLKTPES EVSSFTASKV
160 170 180 190 200
SQYSCQRKTT LNNCNKKFTD AFEIMAENYE FKENEIFCLE FLRAASVLKS
210 220 230 240 250
LPFPVTRMKD IQGLPCMGDR VRDVIEEIIE EGESSRAKDV LNDERYKSFK
260 270 280 290 300
EFTSVFGVGV KTSEKWFRMG LRTVEEVKAD KTLKLSKMQR AGFLYYEDLV
310 320 330 340 350
SCVSKAEADA VSSIVKNTVC TFLPDALVTI TGGFRRGKKI GHDIDFLITS
360 370 380 390 400
PGQREDDELL HKGLLLYCDI IESTFVKEQI PSRHVDAMDH FQKCFAILKL
410 420 430 440 450
YQPRVDNSSY NMSKKCDMAE VKDWKAIRVD LVITPFEQYA YALLGWTGSR
460 470 480 490 500
QFGRDLRRYA THERKMMLDN HALYDKRKRV FLKAGSEEEI FAHLGLDYVE

PWERNA
Length:506
Mass (Da):58,447
Last modified:June 1, 1994 - v1
Checksum:i92B2BFD87B30E94E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06938 mRNA. Translation: AAA75280.1.
PIRiS55786.
RefSeqiNP_990720.1. NM_205389.1.
UniGeneiGga.774.

Genome annotation databases

GeneIDi396351.
KEGGigga:396351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06938 mRNA. Translation: AAA75280.1.
PIRiS55786.
RefSeqiNP_990720.1. NM_205389.1.
UniGeneiGga.774.

3D structure databases

ProteinModelPortaliP36195.
SMRiP36195. Positions 29-125, 149-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000032645.

Proteomic databases

PaxDbiP36195.
PRIDEiP36195.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396351.
KEGGigga:396351.

Organism-specific databases

CTDi1791.

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiP36195.
KOiK00977.
PhylomeDBiP36195.

Miscellaneous databases

PROiP36195.

Family and domain databases

Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "T-cell specific avian TdT: characterization of the cDNA and recombinant enzyme."
    Yang B., Gathy K.N., Coleman M.S.
    Nucleic Acids Res. 23:2041-2048(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Thymus.

Entry informationi

Entry nameiTDT_CHICK
AccessioniPrimary (citable) accession number: P36195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.