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P36193

- DROS_DROME

UniProt

P36193 - DROS_DROME

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Protein

Drosocin

Gene
Dro, CG10816
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Antibacterial peptide with strong anti-Gram-negative bacteria activity.

GO - Biological processi

  1. antibacterial humoral response Source: FlyBase
  2. defense response Source: FlyBase
  3. defense response to bacterium Source: FlyBase
  4. defense response to Gram-negative bacterium Source: UniProtKB
  5. defense response to Gram-positive bacterium Source: FlyBase
  6. innate immune response Source: UniProtKB-KW
  7. positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria Source: UniProtKB
  8. response to bacterium Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Drosocin
Gene namesi
Name:Dro
ORF Names:CG10816
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010388. Dro.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed predictionAdd
BLAST
Propeptidei20 – 212PRO_0000004958
Peptidei22 – 4019Drosocin1 PublicationPRO_0000004959Add
BLAST
Propeptidei43 – 6422 Reviewed predictionPRO_0000004960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281O-linked (GalNAc...)1 Publication
Glycosylationi32 – 321O-linked (GalNAc...)1 Publication

Post-translational modificationi

O-glycosylation is essential for full biological activity.

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Expressioni

Tissue specificityi

In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following two weeks.1 Publication

Developmental stagei

Expressed in larvae and in adults.

Inductioni

By bacterial infection.1 Publication

Gene expression databases

BgeeiP36193.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EZRX-ray1.90B33-40[»]

Family & Domainsi

Sequence similaritiesi

Belongs to the drosocin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG246969.
InParanoidiP36193.
OMAiALAIEDH.
OrthoDBiEOG7P02N0.
PhylomeDBiP36193.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36193-1 [UniParc]FASTAAdd to Basket

« Hide

MKFTIVFLLL ACVFAMGVAT PGKPRPYSPR PTSHPRPIRV RREALAIEDH   50
LTQAAIRPPP ILPA 64
Length:64
Mass (Da):7,085
Last modified:October 5, 2010 - v2
Checksum:iD715F5BA52FDAD17
GO

Sequence cautioni

The sequence AAM29584.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Mass spectrometryi

Molecular mass is 2401.9 Da from positions 22 - 40. Determined by MALDI. With monosaccharide on Thr-32.1 Publication
Molecular mass is 2564.4 Da from positions 22 - 40. Determined by MALDI. With disaccharide on Ser-28 and Thr-32.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti52 – 521T → A in strain: 2CPA1, 2CPA12, 2CPA51, 2CPA105, 2CPA118, 2CPA122, 8B, 8D, 8E, 8F, 8G, 9A, 9B, 9C, 9D, 9E, 9F, 9G, 9H, 10B, 10F, 10G, 10H and Oregon-R. 3 Publications
Natural varianti64 – 641A → V in strain: 2CPA51. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171G → A in CAA79936. 1 Publication
Sequence conflicti17 – 171G → A in CAA67062. 1 Publication
Sequence conflicti17 – 171G → A in AAO72465. 1 Publication
Sequence conflicti17 – 171G → A in AAO72466. 1 Publication
Sequence conflicti17 – 171G → A in AAO72467. 1 Publication
Sequence conflicti17 – 171G → A in AAO72468. 1 Publication
Sequence conflicti17 – 171G → A in AAO72469. 1 Publication
Sequence conflicti17 – 171G → A in AAO72470. 1 Publication
Sequence conflicti17 – 171G → A in AAO72471. 1 Publication
Sequence conflicti17 – 171G → A in AAO72472. 1 Publication
Sequence conflicti17 – 171G → A in AAO72473. 1 Publication
Sequence conflicti17 – 171G → A in AAO72474. 1 Publication
Sequence conflicti17 – 171G → A in AAO72475. 1 Publication
Sequence conflicti17 – 171G → A in AAO72476. 1 Publication
Sequence conflicti17 – 171G → A in AAO72502. 1 Publication
Sequence conflicti17 – 171G → A in AAO72503. 1 Publication
Sequence conflicti17 – 171G → A in AAO72504. 1 Publication
Sequence conflicti17 – 171G → A in AAO72505. 1 Publication
Sequence conflicti17 – 171G → A in AAO72506. 1 Publication
Sequence conflicti17 – 171G → A in AAO72507. 1 Publication
Sequence conflicti17 – 171G → A in AAO72508. 1 Publication
Sequence conflicti17 – 171G → A in AAO72509. 1 Publication
Sequence conflicti17 – 171G → A in AAO72510. 1 Publication
Sequence conflicti17 – 171G → A in AAO72511. 1 Publication
Sequence conflicti17 – 171G → A in AAO72512. 1 Publication
Sequence conflicti17 – 171G → A in AAO72513. 1 Publication
Sequence conflicti17 – 171G → A in AAO72514. 1 Publication
Sequence conflicti17 – 171G → A in AAO72515. 1 Publication
Sequence conflicti17 – 171G → A in AAO72516. 1 Publication
Sequence conflicti17 – 171G → A in AAO72517. 1 Publication
Sequence conflicti17 – 171G → A in AAO72518. 1 Publication
Sequence conflicti17 – 171G → A in AAO72519. 1 Publication
Sequence conflicti17 – 171G → A in AAO72520. 1 Publication
Sequence conflicti17 – 171G → A in AAO72521. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z21942 Genomic DNA. Translation: CAA79936.1.
X98416 Genomic DNA. Translation: CAA67062.1.
AY224606 Genomic DNA. Translation: AAO72465.1.
AY224607 Genomic DNA. Translation: AAO72466.1.
AY224608 Genomic DNA. Translation: AAO72467.1.
AY224609 Genomic DNA. Translation: AAO72468.1.
AY224610 Genomic DNA. Translation: AAO72469.1.
AY224611 Genomic DNA. Translation: AAO72470.1.
AY224612 Genomic DNA. Translation: AAO72471.1.
AY224613 Genomic DNA. Translation: AAO72472.1.
AY224614 Genomic DNA. Translation: AAO72473.1.
AY224615 Genomic DNA. Translation: AAO72474.1.
AY224616 Genomic DNA. Translation: AAO72475.1.
AY224617 Genomic DNA. Translation: AAO72476.1.
AY224643 Genomic DNA. Translation: AAO72502.1.
AY224644 Genomic DNA. Translation: AAO72503.1.
AY224645 Genomic DNA. Translation: AAO72504.1.
AY224646 Genomic DNA. Translation: AAO72505.1.
AY224647 Genomic DNA. Translation: AAO72506.1.
AY224648 Genomic DNA. Translation: AAO72507.1.
AY224649 Genomic DNA. Translation: AAO72508.1.
AY224650 Genomic DNA. Translation: AAO72509.1.
AY224651 Genomic DNA. Translation: AAO72510.1.
AY224652 Genomic DNA. Translation: AAO72511.1.
AY224653 Genomic DNA. Translation: AAO72512.1.
AY224654 Genomic DNA. Translation: AAO72513.1.
AY224655 Genomic DNA. Translation: AAO72514.1.
AY224656 Genomic DNA. Translation: AAO72515.1.
AY224657 Genomic DNA. Translation: AAO72516.1.
AY224658 Genomic DNA. Translation: AAO72517.1.
AY224659 Genomic DNA. Translation: AAO72518.1.
AY224660 Genomic DNA. Translation: AAO72519.1.
AY224661 Genomic DNA. Translation: AAO72520.1.
AY224662 Genomic DNA. Translation: AAO72521.1.
AE013599 Genomic DNA. Translation: AAF58216.1.
AY113579 mRNA. Translation: AAM29584.2. Different initiation.
PIRiS35984. A47103.
RefSeqiNP_001246324.1. NM_001259395.1.
NP_523744.1. NM_079020.4.
UniGeneiDm.19928.

Genome annotation databases

EnsemblMetazoaiFBtr0087436; FBpp0086566; FBgn0010388.
FBtr0309055; FBpp0301076; FBgn0010388.
GeneIDi36635.
KEGGidme:Dmel_CG10816.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z21942 Genomic DNA. Translation: CAA79936.1 .
X98416 Genomic DNA. Translation: CAA67062.1 .
AY224606 Genomic DNA. Translation: AAO72465.1 .
AY224607 Genomic DNA. Translation: AAO72466.1 .
AY224608 Genomic DNA. Translation: AAO72467.1 .
AY224609 Genomic DNA. Translation: AAO72468.1 .
AY224610 Genomic DNA. Translation: AAO72469.1 .
AY224611 Genomic DNA. Translation: AAO72470.1 .
AY224612 Genomic DNA. Translation: AAO72471.1 .
AY224613 Genomic DNA. Translation: AAO72472.1 .
AY224614 Genomic DNA. Translation: AAO72473.1 .
AY224615 Genomic DNA. Translation: AAO72474.1 .
AY224616 Genomic DNA. Translation: AAO72475.1 .
AY224617 Genomic DNA. Translation: AAO72476.1 .
AY224643 Genomic DNA. Translation: AAO72502.1 .
AY224644 Genomic DNA. Translation: AAO72503.1 .
AY224645 Genomic DNA. Translation: AAO72504.1 .
AY224646 Genomic DNA. Translation: AAO72505.1 .
AY224647 Genomic DNA. Translation: AAO72506.1 .
AY224648 Genomic DNA. Translation: AAO72507.1 .
AY224649 Genomic DNA. Translation: AAO72508.1 .
AY224650 Genomic DNA. Translation: AAO72509.1 .
AY224651 Genomic DNA. Translation: AAO72510.1 .
AY224652 Genomic DNA. Translation: AAO72511.1 .
AY224653 Genomic DNA. Translation: AAO72512.1 .
AY224654 Genomic DNA. Translation: AAO72513.1 .
AY224655 Genomic DNA. Translation: AAO72514.1 .
AY224656 Genomic DNA. Translation: AAO72515.1 .
AY224657 Genomic DNA. Translation: AAO72516.1 .
AY224658 Genomic DNA. Translation: AAO72517.1 .
AY224659 Genomic DNA. Translation: AAO72518.1 .
AY224660 Genomic DNA. Translation: AAO72519.1 .
AY224661 Genomic DNA. Translation: AAO72520.1 .
AY224662 Genomic DNA. Translation: AAO72521.1 .
AE013599 Genomic DNA. Translation: AAF58216.1 .
AY113579 mRNA. Translation: AAM29584.2 . Different initiation.
PIRi S35984. A47103.
RefSeqi NP_001246324.1. NM_001259395.1.
NP_523744.1. NM_079020.4.
UniGenei Dm.19928.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4EZR X-ray 1.90 B 33-40 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0087436 ; FBpp0086566 ; FBgn0010388 .
FBtr0309055 ; FBpp0301076 ; FBgn0010388 .
GeneIDi 36635.
KEGGi dme:Dmel_CG10816.

Organism-specific databases

CTDi 36635.
FlyBasei FBgn0010388. Dro.

Phylogenomic databases

eggNOGi NOG246969.
InParanoidi P36193.
OMAi ALAIEDH.
OrthoDBi EOG7P02N0.
PhylomeDBi P36193.

Miscellaneous databases

GenomeRNAii 36635.
NextBioi 799611.

Gene expression databases

Bgeei P36193.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution."
    Bulet P., Dimarcq J.-L., Hetru C., Lagueux M., Charlet M., Hegy G., van Dorsselaer A., Hoffmann J.A.
    J. Biol. Chem. 268:14893-14897(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-40, VARIANT ALA-52, GLYCOSYLATION AT THR-32.
    Strain: Oregon-R.
  2. "Cloning of the gene encoding the antibacterial peptide drosocin involved in Drosophila immunity. Expression studies during the immune response."
    Charlet M., Lagueux M., Reichhart J.-M., Hoffmann D., Braun A., Meister M.
    Eur. J. Biochem. 241:699-706(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-52.
    Strain: Oregon-R.
  3. "Molecular population genetics of inducible antibacterial peptide genes in Drosophila melanogaster."
    Lazzaro B.P., Clark A.G.
    Mol. Biol. Evol. 20:914-923(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-52 AND VAL-64.
    Strain: 10B, 10C, 10D, 10F, 10G, 10H, 2CPA1, 2CPA103, 2CPA105, 2CPA118, 2CPA12, 2CPA122, 2CPA129, 2CPA14, 2CPA43, 2CPA46, 2CPA51, 2CPA7, 8B, 8C, 8D, 8E, 8F, 8G, 9A, 9B, 9C, 9D, 9E, 9F, 9G and 9H.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  7. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  8. "Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study."
    Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A., Hoffmann J.A., Bulet P.
    Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-28, INDUCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY.
  9. "Conformational studies by NMR of the antimicrobial peptide, drosocin, and its non-glycosylated derivative: effects of glycosylation on solution conformation."
    McManus A.M., Otvos L. Jr., Hoffmann R., Craik D.J.
    Biochemistry 38:705-714(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiDROS_DROME
AccessioniPrimary (citable) accession number: P36193
Secondary accession number(s): Q53ZZ5
, Q867T5, Q86BV9, Q9V749
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 5, 2010
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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