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P36193

- DROS_DROME

UniProt

P36193 - DROS_DROME

Protein

Drosocin

Gene

Dro

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Antibacterial peptide with strong anti-Gram-negative bacteria activity.

    GO - Biological processi

    1. antibacterial humoral response Source: FlyBase
    2. defense response Source: FlyBase
    3. defense response to bacterium Source: FlyBase
    4. defense response to Gram-negative bacterium Source: UniProtKB
    5. defense response to Gram-positive bacterium Source: FlyBase
    6. innate immune response Source: UniProtKB-KW
    7. positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria Source: UniProtKB
    8. response to bacterium Source: FlyBase

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial

    Keywords - Biological processi

    Immunity, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Drosocin
    Gene namesi
    Name:Dro
    ORF Names:CG10816
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0010388. Dro.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 2121 PublicationPRO_0000004958
    Peptidei22 – 4019DrosocinPRO_0000004959Add
    BLAST
    Propeptidei43 – 6422Sequence AnalysisPRO_0000004960Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281O-linked (GalNAc...)1 Publication
    Glycosylationi32 – 321O-linked (GalNAc...)1 Publication

    Post-translational modificationi

    O-glycosylation is essential for full biological activity.2 Publications

    Keywords - PTMi

    Cleavage on pair of basic residues, Glycoprotein

    Expressioni

    Tissue specificityi

    In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following two weeks.1 Publication

    Developmental stagei

    Expressed in larvae and in adults.

    Inductioni

    By bacterial infection.1 Publication

    Gene expression databases

    BgeeiP36193.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EZRX-ray1.90B33-40[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the drosocin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG246969.
    InParanoidiP36193.
    OMAiALAIEDH.
    OrthoDBiEOG7P02N0.
    PhylomeDBiP36193.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36193-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFTIVFLLL ACVFAMGVAT PGKPRPYSPR PTSHPRPIRV RREALAIEDH   50
    LTQAAIRPPP ILPA 64
    Length:64
    Mass (Da):7,085
    Last modified:October 5, 2010 - v2
    Checksum:iD715F5BA52FDAD17
    GO

    Sequence cautioni

    The sequence AAM29584.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171G → A in CAA79936. (PubMed:8325867)Curated
    Sequence conflicti17 – 171G → A in CAA67062. (PubMed:8944755)Curated
    Sequence conflicti17 – 171G → A in AAO72465. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72466. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72467. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72468. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72469. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72470. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72471. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72472. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72473. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72474. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72475. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72476. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72502. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72503. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72504. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72505. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72506. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72507. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72508. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72509. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72510. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72511. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72512. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72513. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72514. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72515. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72516. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72517. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72518. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72519. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72520. (PubMed:12716986)Curated
    Sequence conflicti17 – 171G → A in AAO72521. (PubMed:12716986)Curated

    Mass spectrometryi

    Molecular mass is 2401.9 Da from positions 22 - 40. Determined by MALDI. With monosaccharide on Thr-32.1 Publication
    Molecular mass is 2564.4 Da from positions 22 - 40. Determined by MALDI. With disaccharide on Ser-28 and Thr-32.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti52 – 521T → A in strain: 2CPA1, 2CPA12, 2CPA51, 2CPA105, 2CPA118, 2CPA122, 8B, 8D, 8E, 8F, 8G, 9A, 9B, 9C, 9D, 9E, 9F, 9G, 9H, 10B, 10F, 10G, 10H and Oregon-R. 3 Publications
    Natural varianti64 – 641A → V in strain: 2CPA51. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21942 Genomic DNA. Translation: CAA79936.1.
    X98416 Genomic DNA. Translation: CAA67062.1.
    AY224606 Genomic DNA. Translation: AAO72465.1.
    AY224607 Genomic DNA. Translation: AAO72466.1.
    AY224608 Genomic DNA. Translation: AAO72467.1.
    AY224609 Genomic DNA. Translation: AAO72468.1.
    AY224610 Genomic DNA. Translation: AAO72469.1.
    AY224611 Genomic DNA. Translation: AAO72470.1.
    AY224612 Genomic DNA. Translation: AAO72471.1.
    AY224613 Genomic DNA. Translation: AAO72472.1.
    AY224614 Genomic DNA. Translation: AAO72473.1.
    AY224615 Genomic DNA. Translation: AAO72474.1.
    AY224616 Genomic DNA. Translation: AAO72475.1.
    AY224617 Genomic DNA. Translation: AAO72476.1.
    AY224643 Genomic DNA. Translation: AAO72502.1.
    AY224644 Genomic DNA. Translation: AAO72503.1.
    AY224645 Genomic DNA. Translation: AAO72504.1.
    AY224646 Genomic DNA. Translation: AAO72505.1.
    AY224647 Genomic DNA. Translation: AAO72506.1.
    AY224648 Genomic DNA. Translation: AAO72507.1.
    AY224649 Genomic DNA. Translation: AAO72508.1.
    AY224650 Genomic DNA. Translation: AAO72509.1.
    AY224651 Genomic DNA. Translation: AAO72510.1.
    AY224652 Genomic DNA. Translation: AAO72511.1.
    AY224653 Genomic DNA. Translation: AAO72512.1.
    AY224654 Genomic DNA. Translation: AAO72513.1.
    AY224655 Genomic DNA. Translation: AAO72514.1.
    AY224656 Genomic DNA. Translation: AAO72515.1.
    AY224657 Genomic DNA. Translation: AAO72516.1.
    AY224658 Genomic DNA. Translation: AAO72517.1.
    AY224659 Genomic DNA. Translation: AAO72518.1.
    AY224660 Genomic DNA. Translation: AAO72519.1.
    AY224661 Genomic DNA. Translation: AAO72520.1.
    AY224662 Genomic DNA. Translation: AAO72521.1.
    AE013599 Genomic DNA. Translation: AAF58216.1.
    AY113579 mRNA. Translation: AAM29584.2. Different initiation.
    PIRiS35984. A47103.
    RefSeqiNP_001246324.1. NM_001259395.1.
    NP_523744.1. NM_079020.4.
    UniGeneiDm.19928.

    Genome annotation databases

    EnsemblMetazoaiFBtr0087436; FBpp0086566; FBgn0010388.
    FBtr0309055; FBpp0301076; FBgn0010388.
    GeneIDi36635.
    KEGGidme:Dmel_CG10816.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21942 Genomic DNA. Translation: CAA79936.1 .
    X98416 Genomic DNA. Translation: CAA67062.1 .
    AY224606 Genomic DNA. Translation: AAO72465.1 .
    AY224607 Genomic DNA. Translation: AAO72466.1 .
    AY224608 Genomic DNA. Translation: AAO72467.1 .
    AY224609 Genomic DNA. Translation: AAO72468.1 .
    AY224610 Genomic DNA. Translation: AAO72469.1 .
    AY224611 Genomic DNA. Translation: AAO72470.1 .
    AY224612 Genomic DNA. Translation: AAO72471.1 .
    AY224613 Genomic DNA. Translation: AAO72472.1 .
    AY224614 Genomic DNA. Translation: AAO72473.1 .
    AY224615 Genomic DNA. Translation: AAO72474.1 .
    AY224616 Genomic DNA. Translation: AAO72475.1 .
    AY224617 Genomic DNA. Translation: AAO72476.1 .
    AY224643 Genomic DNA. Translation: AAO72502.1 .
    AY224644 Genomic DNA. Translation: AAO72503.1 .
    AY224645 Genomic DNA. Translation: AAO72504.1 .
    AY224646 Genomic DNA. Translation: AAO72505.1 .
    AY224647 Genomic DNA. Translation: AAO72506.1 .
    AY224648 Genomic DNA. Translation: AAO72507.1 .
    AY224649 Genomic DNA. Translation: AAO72508.1 .
    AY224650 Genomic DNA. Translation: AAO72509.1 .
    AY224651 Genomic DNA. Translation: AAO72510.1 .
    AY224652 Genomic DNA. Translation: AAO72511.1 .
    AY224653 Genomic DNA. Translation: AAO72512.1 .
    AY224654 Genomic DNA. Translation: AAO72513.1 .
    AY224655 Genomic DNA. Translation: AAO72514.1 .
    AY224656 Genomic DNA. Translation: AAO72515.1 .
    AY224657 Genomic DNA. Translation: AAO72516.1 .
    AY224658 Genomic DNA. Translation: AAO72517.1 .
    AY224659 Genomic DNA. Translation: AAO72518.1 .
    AY224660 Genomic DNA. Translation: AAO72519.1 .
    AY224661 Genomic DNA. Translation: AAO72520.1 .
    AY224662 Genomic DNA. Translation: AAO72521.1 .
    AE013599 Genomic DNA. Translation: AAF58216.1 .
    AY113579 mRNA. Translation: AAM29584.2 . Different initiation.
    PIRi S35984. A47103.
    RefSeqi NP_001246324.1. NM_001259395.1.
    NP_523744.1. NM_079020.4.
    UniGenei Dm.19928.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EZR X-ray 1.90 B 33-40 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0087436 ; FBpp0086566 ; FBgn0010388 .
    FBtr0309055 ; FBpp0301076 ; FBgn0010388 .
    GeneIDi 36635.
    KEGGi dme:Dmel_CG10816.

    Organism-specific databases

    CTDi 36635.
    FlyBasei FBgn0010388. Dro.

    Phylogenomic databases

    eggNOGi NOG246969.
    InParanoidi P36193.
    OMAi ALAIEDH.
    OrthoDBi EOG7P02N0.
    PhylomeDBi P36193.

    Miscellaneous databases

    GenomeRNAii 36635.
    NextBioi 799611.

    Gene expression databases

    Bgeei P36193.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution."
      Bulet P., Dimarcq J.-L., Hetru C., Lagueux M., Charlet M., Hegy G., van Dorsselaer A., Hoffmann J.A.
      J. Biol. Chem. 268:14893-14897(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-40, VARIANT ALA-52, GLYCOSYLATION AT THR-32.
      Strain: Oregon-R.
    2. "Cloning of the gene encoding the antibacterial peptide drosocin involved in Drosophila immunity. Expression studies during the immune response."
      Charlet M., Lagueux M., Reichhart J.-M., Hoffmann D., Braun A., Meister M.
      Eur. J. Biochem. 241:699-706(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-52.
      Strain: Oregon-R.
    3. "Molecular population genetics of inducible antibacterial peptide genes in Drosophila melanogaster."
      Lazzaro B.P., Clark A.G.
      Mol. Biol. Evol. 20:914-923(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-52 AND VAL-64.
      Strain: 10B, 10C, 10D, 10F, 10G, 10H, 2CPA1, 2CPA103, 2CPA105, 2CPA118, 2CPA12, 2CPA122, 2CPA129, 2CPA14, 2CPA43, 2CPA46, 2CPA51, 2CPA7, 8B, 8C, 8D, 8E, 8F, 8G, 9A, 9B, 9C, 9D, 9E, 9F, 9G and 9H.
    4. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    5. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Head.
    7. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    8. "Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study."
      Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A., Hoffmann J.A., Bulet P.
      Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-28, INDUCTION, MASS SPECTROMETRY, TISSUE SPECIFICITY.
    9. "Conformational studies by NMR of the antimicrobial peptide, drosocin, and its non-glycosylated derivative: effects of glycosylation on solution conformation."
      McManus A.M., Otvos L. Jr., Hoffmann R., Craik D.J.
      Biochemistry 38:705-714(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiDROS_DROME
    AccessioniPrimary (citable) accession number: P36193
    Secondary accession number(s): Q53ZZ5
    , Q867T5, Q86BV9, Q9V749
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3