P36187 (TPI2_GIAIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Triosephosphate isomerase Short name=TIM EC=5.3.1.1 Alternative name(s): Triose-phosphate isomerase
Organism	Giardia intestinalis (Giardia lamblia)
Taxonomic identifier	5741 [NCBI]
Taxonomic lineage	Eukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Triosephosphate isomerase		PRO_0000090136
Sites
	Active site	96	1	Electrophile By similarity
	Active site	170	1	Proton acceptor By similarity
	Binding site	11	1	Substrate By similarity
	Binding site	13	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36187 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B23006F46E5BE1C6	FASTA	257	27,851
	10 20 30 40 50 60 MPARRPFIGG NFKCNGSLDF IKSHVASIAS YKIPESVDVV VAPSFVHLST AIAANTSKCL 70 80 90 100 110 120 KIAAQNVYLE GNGAWTGETS VEMLLDMGLS HVIIGHSERR RIMGETNEQS AKKAKRALDK 130 140 150 160 170 180 GMTVIFCTGE TLDERKANNT MEVNIAQLEA LKKEIGESKK LWENVVIAYE PVWSIGTGVV 190 200 210 220 230 240 ATPEQAEEVH VGLRKWFAEK VCAEGAQHIR IIYGGSANGS NCEKLGQCPN IDGFLVGGAS 250 LKPEFTTMID ILAKTRA

References

[1]	"Complementation of an Escherichia coli glycolysis mutant by Giardia lamblia triosephosphate isomerase." Mowatt M.R., Weinbach E.C., Howard T.C., Nash T.E. Exp. Parasitol. 78:85-92(1994) [PubMed: 8299763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02116 Unassigned DNA. Translation: AAA18205.1.
3D structure databases
ProteinModelPortal	P36187.
SMR	P36187. Positions 2-256.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPI2_GIAIN

Accession

Primary (citable) accession number: P36187

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	October 19, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)