Reviewed, UniProtKB/Swiss-Prot P36186 (TPI1_GIALA)

Names and origin

Protein names	Recommended name:     Triosephosphate isomerase       Short name=TIM     EC=5.3.1.1 Alternative name(s):     Triose-phosphate isomerase
Organism	Giardia lamblia (Giardia intestinalis)
Taxonomic identifier	5741 [NCBI]
Taxonomic lineage	Eukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Gluconeogenesis Glycolysis Lipid synthesis Pentose shunt
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 257

257

Triosephosphate isomerase

PRO_0000090135

Sites

Active site

96

1

Electrophile By similarity

Active site

170

1

Proton acceptor By similarity

Binding site

11

1

Substrate By similarity

Binding site

13

1

Substrate By similarity

Secondary structure

1

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257

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

18 – 29

12

Beta strand

37 – 42

6

Helix

45 – 47

3

Helix

48 – 54

7

Beta strand

61 – 65

5

Beta strand

72 – 74

3

Helix

81 – 86

6

Beta strand

91 – 94

4

Helix

97 – 101

5

Helix

107 – 119

13

Beta strand

123 – 128

6

Helix

132 – 136

5

Helix

140 – 155

16

Helix

159 – 164

6

Beta strand

165 – 169

5

Helix

172 – 174

3

Turn

175 – 177

3

Helix

183 – 200

18

Helix

203 – 208

6

Beta strand

209 – 216

8

Turn

219 – 221

3

Helix

222 – 226

5

Beta strand

233 – 237

5

Helix

238 – 241

4

Helix

244 – 255

12

Sequences

	Sequence		Length	Mass (Da)
	P36186-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 7CD7AC0AB1FB847A	FASTA	257	27,903
	10 20 30 40 50 60 MPARRPFIGG NFKCNGSLDF IKSHVAAIAA HKIPDSVDVV IAPSAVHLST AIAANTSKQL 70 80 90 100 110 120 RIAAQNVYLE GNGAWTGETS VEMLQDMGLK HVIVGHSERR RIMGETDEQS AKKAKRALEK 130 140 150 160 170 180 GMTVIFCVGE TLDERKANRT MEVNIAQLEA LGKELGESKM LWKEVVIAYE PVWSIGTGVV 190 200 210 220 230 240 ATPEQAEEVH VGLRKWFAEK VCAEGAQHIR IIYGGSANGS NCEKLGQCPN IDGFLVGGAS 250 LKPEFMTMID ILTKTRT

References

[1]	"Complementation of an Escherichia coli glycolysis mutant by Giardia lamblia triosephosphate isomerase." Mowatt M.R., Weinbach E.C., Howard T.C., Nash T.E. Exp. Parasitol. 78:85-92(1994) [PubMed: 8299763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 30957 / WB.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L02120 Unassigned DNA. Translation: AAA18203.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DP3	X-ray	2.10	A	1-257	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

5.3.1.1. 322.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR000652. Triosephosphate_isomerase.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR21139. Triophos_ismrse. 1 hit.

Pfam

PF00121. TIM. 1 hit.
[Graphical view]

ProDom

PD001005. Triophos_ismrse. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00419. tim. 1 hit.

PROSITE

PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPI1_GIALA

Accession

Primary (citable) accession number: P36186

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)