P36183 (ENPL_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoplasmin homolog Alternative name(s): Glucose-regulated protein 94 homolog Short name=GRP-94 homolog
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a molecular chaperone role in the processing of secreted materials By similarity.
Subcellular location	Endoplasmic reticulum lumen.
Induction	Accumulates rapidly in leaves upon heat shock treatment and during infection by a pathogen.
Sequence similarities	Belongs to the heat shock protein 90 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Chaperone
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 809	791	Endoplasmin homolog		PRO_0000013603
Regions
	Motif	806 – 809	4	Prevents secretion from ER
Sites
	Binding site	111	1	ATP By similarity
	Binding site	155	1	ATP By similarity
	Binding site	168	1	ATP By similarity
	Binding site	174	1	ATP By similarity
	Binding site	200	1	ATP; via amide nitrogen By similarity
	Binding site	453	1	ATP By similarity
Amino acid modifications
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	410	1	N-linked (GlcNAc...) Potential
	Glycosylation	450	1	N-linked (GlcNAc...) Potential
	Glycosylation	617	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P36183 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 79798FDBC15B44D0	FASTA	809	92,917
	10 20 30 40 50 60 MRKWALSCAL LLVLLLTTLP DPAKKLQVNA EESSDEVGDF PKVEEKLGAV PHGLSTDSEV 70 80 90 100 110 120 VQRESESISR KTLRNSAEKF EFQAEVSRLM DIIINSLYSN KDIFLRELIS NASDALDKIR 130 140 150 160 170 180 FLALTDKEVM GEGDTAKLEI QIKLDKENKI LSIRDRGVGM TKEDLIKNLG TIAKSGTSAF 190 200 210 220 230 240 VEKMQTGGDL NLIGQFGVGF YSVYLVADYV EVVSKHNDDK QYVWESKADG SFAISEDTWN 250 260 270 280 290 300 EPLGRGTEIK LHLRDEAKEY LEEGKLKDLV KKYSEFINFP IYLWATKEVD VEVPADEEES 310 320 330 340 350 360 NEEEESTTET TEEEETEDDE EKKPKTKTVK ETTTEWELLN DMKAVWLRSP KEVTEEEYAK 370 380 390 400 410 420 FYHSLAKDFG DDKPMSWSHF SAEGDVEFKA LLFVPPKAPH DLYESYYNAN KSNLKLYVRR 430 440 450 460 470 480 VFISDEFDDL LPKYLSFLMG IVDSDTLPLN VSREMLQQHS SLKTIKKKLI RKALDMIRKL 490 500 510 520 530 540 AEEDPDEYSN KEKTDDEKSA MEEKKGQYAK FWNEFGKSVK LGIIEDATNR NRLAKLLRFE 550 560 570 580 590 600 SSKSDGKLVS LDEYISRMKS GQKDIFYLTG SSKEQLEKSP FLEQLTKKNY EVIYFTDPVD 610 620 630 640 650 660 EYLMQYLMDY EDKKFQNVSK EGLKLGKDSK LKDLKESFKE LTDWWKKALD TEGIDSVKIS 670 680 690 700 710 720 NRLHNTPCVV VTSKYGWSSN MEKIMQAQTL SDASKQAYMR GKRVLEINPR HPIIKELRDK 730 740 750 760 770 780 VAQDSDSEGL KQTARLVYQT ALMESGFNLP DPKDFASSIY RSVQKSLDLS PDAAVEEEEE 790 800 VEEPEVEEKE SAKQEAEEPE HEQYDKDEL

References

[1]

"A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum." Walther-Larsen H., Brandt J., Collinge D.B., Thordal-Christensen H. Plant Mol. Biol. 21:1097-1108(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Pallas / P-01. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67960 mRNA. Translation: CAA48143.1.
PIR	S33533.
UniGene	Hv.26181.
3D structure databases
ProteinModelPortal	P36183.
SMR	P36183. Positions 77-287.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-737N.
Proteomic databases
PRIDE	P36183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P36183.
Gene expression databases
Genevestigator	P36183.
Family and domain databases
Gene3D	3.30.565.10. 1 hit.
InterPro	IPR015566. Endoplasmin. IPR003594. HATPase_ATP-bd. IPR019805. Heat_shock_protein_90_CS. IPR001404. Hsp90. IPR020575. Hsp90_N. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
PANTHER	PTHR11528. PTHR11528. 1 hit. PTHR11528:SF21. PTHR11528:SF21. 1 hit.
Pfam	PF00183. HSP90. 1 hit. [Graphical view]
PIRSF	PIRSF002583. Hsp90. 1 hit.
PRINTS	PR00775. HEATSHOCK90.
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00298. HSP90. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENPL_HORVU

Accession

Primary (citable) accession number: P36183

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program