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Protein

Serine/threonine-protein phosphatase PP2A 65 kDa regulatory subunit

Gene

Pp2A-29B

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.By similarity

GO - Molecular functioni

  • protein phosphatase type 2A regulator activity Source: FlyBase

GO - Biological processi

  • autophagy Source: FlyBase
  • centriole replication Source: FlyBase
  • centrosome cycle Source: FlyBase
  • centrosome duplication Source: FlyBase
  • centrosome organization Source: FlyBase
  • chromosome segregation Source: FlyBase
  • neurogenesis Source: FlyBase
  • phagocytosis Source: FlyBase
  • protein complex assembly Source: InterPro
  • protein dephosphorylation Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • spindle assembly Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-196299. Beta-catenin phosphorylation cascade.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A 65 kDa regulatory subunit
Alternative name(s):
PR65
Protein phosphatase PP2A regulatory subunit A
Gene namesi
ORF Names:CG17291
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0260439. Pp2A-29B.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: FlyBase
  • cytoplasm Source: FlyBase
  • protein phosphatase type 2A complex Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000714082 – 591Serine/threonine-protein phosphatase PP2A 65 kDa regulatory subunitAdd BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP36179.
PRIDEiP36179.

Expressioni

Tissue specificityi

Expression varies in tissues throughout development. Highly distributed expression in early embryos. In late embryonal development, found at high levels in nervous system and gonads. In third instar larvae, found in brain, imaginal disks and salivary glands.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Expressed at lower levels in larvae and adult.1 Publication

Gene expression databases

BgeeiFBgn0260439.
ExpressionAtlasiP36179. baseline.
GenevisibleiP36179. DM.

Interactioni

Subunit structurei

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Protein-protein interaction databases

BioGridi77789. 68 interactors.
IntActiP36179. 9 interactors.
MINTiMINT-803219.
STRINGi7227.FBpp0297239.

Structurei

3D structure databases

ProteinModelPortaliP36179.
SMRiP36179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati10 – 48HEAT 1Add BLAST39
Repeati49 – 86HEAT 2Add BLAST38
Repeati87 – 125HEAT 3Add BLAST39
Repeati126 – 163HEAT 4Add BLAST38
Repeati164 – 202HEAT 5Add BLAST39
Repeati203 – 241HEAT 6Add BLAST39
Repeati242 – 280HEAT 7Add BLAST39
Repeati281 – 323HEAT 8Add BLAST43
Repeati324 – 362HEAT 9Add BLAST39
Repeati363 – 401HEAT 10Add BLAST39
Repeati402 – 440HEAT 11Add BLAST39
Repeati441 – 479HEAT 12Add BLAST39
Repeati480 – 518HEAT 13Add BLAST39
Repeati519 – 557HEAT 14Add BLAST39
Repeati558 – 591HEAT 15Add BLAST34

Domaini

Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

Sequence similaritiesi

Contains 15 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0211. Eukaryota.
ENOG410XQVI. LUCA.
GeneTreeiENSGT00730000110944.
InParanoidiP36179.
KOiK03456.
OrthoDBiEOG091G045V.
PhylomeDBiP36179.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR031090. PP2A_A_meta.
[Graphical view]
PANTHERiPTHR10648:SF9. PTHR10648:SF9. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 11 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASDKSVDD SLYPIAVLID ELKNEDVQLR LNSIKKLSTI ALALGEERTR
60 70 80 90 100
SELIPFLTET IYDEDEVLLA LADQLGNFTS LVGGPEFAMY LIPPLESLAT
110 120 130 140 150
VEETVVRDKA VESLRTVAAE HSAQDLEIHV VPTLQRLVSG DWFTSRTSAC
160 170 180 190 200
GLFSVCYPRV TQPVKAELRA NFRKLCQDET PMVRRAAANK LGEFAKVVET
210 220 230 240 250
EYLKSDLIPN FVQLAQDDQD SVRLLAVEAC VSIAQLLPQD DVEHLVLPTL
260 270 280 290 300
RQCASDSSWR VRYMVAEKFV DLQKAVGPEI TRVDLVPAFQ YLLKDAEAEV
310 320 330 340 350
RAAVATKVKD FCANLDKVNQ VQIILSSILP YVRDLVSDPN PHVKSALASV
360 370 380 390 400
IMGLSPMLGA YQTVEQLLPL FLIQLKDECP EVRLNIISNL DCVNDVIGIQ
410 420 430 440 450
QLSQSLLPAI VELAEDSKWR VRLAIIEYMP ALAGQLGQEF FDQKLRGLCM
460 470 480 490 500
GWLNDHVYAI REAATLNMKK LVEQFGAPWA EQAIIPMILV MSRNKNYLHR
510 520 530 540 550
MTCLFCLNVL AEVCGTDITT KLLLPTVLLL AADPVANVRF NVAKTLQKIS
560 570 580 590
PFLEASVIDA QVKPTLDKLN TDTDVDVKHF AAQAIAGIAA A
Length:591
Mass (Da):65,424
Last modified:January 23, 2007 - v4
Checksum:i13948F39796662AC
GO

Sequence cautioni

The sequence AAX33553 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232S → T in AAA28304 (PubMed:1320961).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86442 mRNA. Translation: AAA28304.1.
AE014134 Genomic DNA. Translation: AAF52650.2.
AE014134 Genomic DNA. Translation: AAZ66587.1.
BT021405 mRNA. Translation: AAX33553.1. Sequence problems.
PIRiA43767.
RefSeqiNP_001027225.1. NM_001032054.3.
NP_001027226.1. NM_001032055.3.
NP_001027227.1. NM_001032056.3.
UniGeneiDm.1532.

Genome annotation databases

EnsemblMetazoaiFBtr0005088; FBpp0099974; FBgn0260439.
FBtr0100533; FBpp0099975; FBgn0260439.
FBtr0100535; FBpp0293227; FBgn0260439.
GeneIDi2768940.
KEGGidme:Dmel_CG17291.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86442 mRNA. Translation: AAA28304.1.
AE014134 Genomic DNA. Translation: AAF52650.2.
AE014134 Genomic DNA. Translation: AAZ66587.1.
BT021405 mRNA. Translation: AAX33553.1. Sequence problems.
PIRiA43767.
RefSeqiNP_001027225.1. NM_001032054.3.
NP_001027226.1. NM_001032055.3.
NP_001027227.1. NM_001032056.3.
UniGeneiDm.1532.

3D structure databases

ProteinModelPortaliP36179.
SMRiP36179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi77789. 68 interactors.
IntActiP36179. 9 interactors.
MINTiMINT-803219.
STRINGi7227.FBpp0297239.

Proteomic databases

PaxDbiP36179.
PRIDEiP36179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0005088; FBpp0099974; FBgn0260439.
FBtr0100533; FBpp0099975; FBgn0260439.
FBtr0100535; FBpp0293227; FBgn0260439.
GeneIDi2768940.
KEGGidme:Dmel_CG17291.

Organism-specific databases

CTDi2768940.
FlyBaseiFBgn0260439. Pp2A-29B.

Phylogenomic databases

eggNOGiKOG0211. Eukaryota.
ENOG410XQVI. LUCA.
GeneTreeiENSGT00730000110944.
InParanoidiP36179.
KOiK03456.
OrthoDBiEOG091G045V.
PhylomeDBiP36179.

Enzyme and pathway databases

ReactomeiR-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-196299. Beta-catenin phosphorylation cascade.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

GenomeRNAii2768940.
PROiP36179.

Gene expression databases

BgeeiFBgn0260439.
ExpressionAtlasiP36179. baseline.
GenevisibleiP36179. DM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR031090. PP2A_A_meta.
[Graphical view]
PANTHERiPTHR10648:SF9. PTHR10648:SF9. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 11 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei2AAA_DROME
AccessioniPrimary (citable) accession number: P36179
Secondary accession number(s): A4V0F7
, Q2MGK6, Q5BI19, Q9VLN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.