P36175 (GCP_PASHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp

Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein

Gene names

Name:

gcp

Organism

Pasteurella haemolytica (Mannheimia haemolytica)

Taxonomic identifier

75985 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Mannheimia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP MF_01445
Subcellular location	Secreted. Note: Not secreted through the conventional secretory pathway since it lacks a signal sequence. HAMAP MF_01445
Sequence similarities	Belongs to the KAE1 / YgjD family.
Caution	Was originally thought to have endopeptidase activity (Ref.1). But it could not be confirmed with orthologs purified from P. abyssi (PubMed:17766251, PubMed:21183954).

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Secreted
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp HAMAP MF_01445		PRO_0000096976

Sequences

	Sequence		Length	Mass (Da)
	P36175 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 161D58F60AD1D589	FASTA	325	35,190
	10 20 30 40 50 60 MRILGIETSC DETGVAIYDE DKGLVANQLY SQIDMHADYG GVVPELASRD HIRKTLPLIQ 70 80 90 100 110 120 EALKEANLQP SDIDGIAYTA GPGLVGALLV GSTIARSLAY AWNVPALGVH HMEGHLLAPM 130 140 150 160 170 180 LEENAPEFPF VALLISGGHT QLVKVDGVGQ YELLGESIDD AAGEAFDKTG KLLGLDYPAG 190 200 210 220 230 240 VAMSKLAESG TPNRFKFPRP MTDRPGLDFS FSGLKTFAAN TIKANLNENG ELDEQTKCDI 250 260 270 280 290 300 AHAFQQAVVD TILIKCKRAL EQTGYKRLVM AGGVSANKQL RADLAEMMKK LKGEVFYPRP 310 320 QFCTDNGAMI AYTGFLRLKT MNKPT

References

[1]	"Cloning, nucleotide sequence, and expression of the Pasteurella haemolytica A1 glycoprotease gene." Abdullah K.M., Lo R.Y.C., Mellors A. J. Bacteriol. 173:5597-5603(1991) [PubMed: 1885539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: Serotype A1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15958 Genomic DNA. Translation: AAA80282.1.
PIR	A38108.
3D structure databases
ProteinModelPortal	P36175.
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01445. gcp. [Tree]
InterPro	IPR022450. Pept_M22_O-sialoglycopept. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03723. Bact_gcp. 1 hit. TIGR00329. Gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_PASHA

Accession

Primary (citable) accession number: P36175

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 21, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program