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P36173

- GEX2_YEAST

UniProt

P36173 - GEX2_YEAST

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Protein

Glutathione exchanger 2

Gene

GEX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway.1 Publication

GO - Molecular functioni

  1. solute:proton antiporter activity Source: SGD

GO - Biological processi

  1. glutathione transmembrane transport Source: SGD
  2. hydrogen ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32068-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione exchanger 2
Gene namesi
Name:GEX2
Ordered Locus Names:YKR106W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR106w.
SGDiS000001814. GEX2.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5858CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei59 – 7921HelicalSequence AnalysisAdd
BLAST
Topological domaini80 – 12041ExtracellularSequence AnalysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence AnalysisAdd
BLAST
Topological domaini142 – 15211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Topological domaini174 – 21643ExtracellularSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Topological domaini238 – 27538CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence AnalysisAdd
BLAST
Topological domaini297 – 30711ExtracellularSequence AnalysisAdd
BLAST
Transmembranei308 – 32821HelicalSequence AnalysisAdd
BLAST
Topological domaini329 – 34315CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei344 – 36421HelicalSequence AnalysisAdd
BLAST
Topological domaini365 – 38319ExtracellularSequence AnalysisAdd
BLAST
Transmembranei384 – 40421HelicalSequence AnalysisAdd
BLAST
Topological domaini405 – 4073CytoplasmicSequence Analysis
Transmembranei408 – 42821HelicalSequence AnalysisAdd
BLAST
Topological domaini429 – 44012ExtracellularSequence AnalysisAdd
BLAST
Transmembranei441 – 46121HelicalSequence AnalysisAdd
BLAST
Topological domaini462 – 47110CytoplasmicSequence Analysis
Transmembranei472 – 49221HelicalSequence AnalysisAdd
BLAST
Topological domaini493 – 54856ExtracellularSequence AnalysisAdd
BLAST
Transmembranei549 – 56921HelicalSequence AnalysisAdd
BLAST
Topological domaini570 – 61546CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
  3. vacuolar membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Glutathione exchanger 2PRO_0000203231Add
BLAST

Expressioni

Inductioni

By iron depletion. Expressed at a very low level.1 Publication

Gene expression databases

GenevestigatoriP36173.

Interactioni

Protein-protein interaction databases

BioGridi34237. 9 interactions.
DIPiDIP-4917N.
MINTiMINT-497647.
STRINGi4932.YKR106W.

Structurei

3D structure databases

ProteinModelPortaliP36173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG281153.
GeneTreeiENSGT00390000002930.
InParanoidiP36173.
OMAiRIMVISS.
OrthoDBiEOG7PZS6Z.

Family and domain databases

InterProiIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.

Sequencei

Sequence statusi: Complete.

P36173-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS
60 70 80 90 100
AQFDSLKYKI LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV
110 120 130 140 150
QVINAVVSVG SQVVYSRLSD HFGRLRLFLV ATIFYIMGTI IQSQATRLTM
160 170 180 190 200
YAAGSVFYNC GYVGTNLLLT LILSDFSSLK WRMFYQYASY WPYIIIPWIS
210 220 230 240 250
GNIITAANPQ KNWSWNIAMW AFIYPLSTLP IIFLILYMKY KSSKTAEWRS
260 270 280 290 300
LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS
310 320 330 340 350
QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG
360 370 380 390 400
VTFFNFFTFF ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS
410 420 430 440 450
LLVAKTRKLK LSVIGGCAAW MVCMGLFYKY RGGSGSHEGV IAASVIMGLS
460 470 480 490 500
GLLCSNSVIV ILQAMTTHSR MAVITGIQYT FSKLGAAIGA SVSGAIWTQT
510 520 530 540 550
MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV ESYRYVQRII
560 570 580 590 600
MTVGLACTVP FFTFTMFMRN PELIDKATHE EFTEDGLVVL PDEENIFSQI
610
KALFRHNRSN KKSGC
Length:615
Mass (Da):68,954
Last modified:June 1, 1994 - v1
Checksum:iB548FBC6E49326AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28202 Genomic DNA. Translation: CAA82047.1.
BK006944 Genomic DNA. Translation: DAA09257.1.
PIRiS40624.
RefSeqiNP_013032.1. NM_001179896.1.

Genome annotation databases

EnsemblFungiiYKR106W; YKR106W; YKR106W.
GeneIDi853981.
KEGGisce:YKR106W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28202 Genomic DNA. Translation: CAA82047.1 .
BK006944 Genomic DNA. Translation: DAA09257.1 .
PIRi S40624.
RefSeqi NP_013032.1. NM_001179896.1.

3D structure databases

ProteinModelPortali P36173.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34237. 9 interactions.
DIPi DIP-4917N.
MINTi MINT-497647.
STRINGi 4932.YKR106W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR106W ; YKR106W ; YKR106W .
GeneIDi 853981.
KEGGi sce:YKR106W.

Organism-specific databases

CYGDi YKR106w.
SGDi S000001814. GEX2.

Phylogenomic databases

eggNOGi NOG281153.
GeneTreei ENSGT00390000002930.
InParanoidi P36173.
OMAi RIMVISS.
OrthoDBi EOG7PZS6Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-32068-MONOMER.

Miscellaneous databases

NextBioi 975446.

Gene expression databases

Genevestigatori P36173.

Family and domain databases

InterProi IPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  4. "Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis."
    Dhaoui M., Auchere F., Blaiseau P.L., Lesuisse E., Landoulsi A., Camadro J.M., Haguenauer-Tsapis R., Belgareh-Touze N.
    Mol. Biol. Cell 22:2054-2067(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGEX2_YEAST
AccessioniPrimary (citable) accession number: P36173
Secondary accession number(s): D6VXG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3