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P36173 (GEX2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione exchanger 2
Gene names
Name:GEX2
Ordered Locus Names:YKR106W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proton/glutathione antiporter that imports glutathione from the vacuole and exports it through the plasma membrane. Involved in resistance to oxidative stress and modulation of the PKA pathway. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein Ref.4.

Induction

By iron depletion. Expressed at a very low level. Ref.4

Sequence similarities

Belongs to the major facilitator superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615Glutathione exchanger 2
PRO_0000203231

Regions

Topological domain1 – 5858Cytoplasmic Potential
Transmembrane59 – 7921Helical; Potential
Topological domain80 – 12041Extracellular Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15211Cytoplasmic Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 21643Extracellular Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 27538Cytoplasmic Potential
Transmembrane276 – 29621Helical; Potential
Topological domain297 – 30711Extracellular Potential
Transmembrane308 – 32821Helical; Potential
Topological domain329 – 34315Cytoplasmic Potential
Transmembrane344 – 36421Helical; Potential
Topological domain365 – 38319Extracellular Potential
Transmembrane384 – 40421Helical; Potential
Topological domain405 – 4073Cytoplasmic Potential
Transmembrane408 – 42821Helical; Potential
Topological domain429 – 44012Extracellular Potential
Transmembrane441 – 46121Helical; Potential
Topological domain462 – 47110Cytoplasmic Potential
Transmembrane472 – 49221Helical; Potential
Topological domain493 – 54856Extracellular Potential
Transmembrane549 – 56921Helical; Potential
Topological domain570 – 61546Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P36173 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B548FBC6E49326AD

FASTA61568,954
        10         20         30         40         50         60 
MSSSVVGASS NKKSGIRQSC EIIERERHSN DDTYSMTSTF FKLKENEIMS AQFDSLKYKI 

        70         80         90        100        110        120 
LLISTAFVCG FGISLDYTLR STYTGYATNS YSEHSLLSTV QVINAVVSVG SQVVYSRLSD 

       130        140        150        160        170        180 
HFGRLRLFLV ATIFYIMGTI IQSQATRLTM YAAGSVFYNC GYVGTNLLLT LILSDFSSLK 

       190        200        210        220        230        240 
WRMFYQYASY WPYIIIPWIS GNIITAANPQ KNWSWNIAMW AFIYPLSTLP IIFLILYMKY 

       250        260        270        280        290        300 
KSSKTAEWRS LKEQARKERT GGLFENLVFL FWKLDIVGIL LITVSLGCIL VPLTLANETS 

       310        320        330        340        350        360 
QKWHNSKIIA TLVSGGCLFF IFLYWEAKFA KSPLLPFKLL SDRGIWAPLG VTFFNFFTFF 

       370        380        390        400        410        420 
ISCDYLYPVL LVSMKESSTS AARIVNLPDF VAATASPFYS LLVAKTRKLK LSVIGGCAAW 

       430        440        450        460        470        480 
MVCMGLFYKY RGGSGSHEGV IAASVIMGLS GLLCSNSVIV ILQAMTTHSR MAVITGIQYT 

       490        500        510        520        530        540 
FSKLGAAIGA SVSGAIWTQT MPNQLYKNLG NDTLAEIAYA SPYTFISDYP WGSPERDAVV 

       550        560        570        580        590        600 
ESYRYVQRII MTVGLACTVP FFTFTMFMRN PELIDKATHE EFTEDGLVVL PDEENIFSQI 

       610 
KALFRHNRSN KKSGC

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[4]"Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis."
Dhaoui M., Auchere F., Blaiseau P.L., Lesuisse E., Landoulsi A., Camadro J.M., Haguenauer-Tsapis R., Belgareh-Touze N.
Mol. Biol. Cell 22:2054-2067(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z28202 Genomic DNA. Translation: CAA82047.1.
BK006944 Genomic DNA. Translation: DAA09257.1.
PIRS40624.
RefSeqNP_013032.1. NM_001179896.1.

3D structure databases

ProteinModelPortalP36173.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4917N.
MINTMINT-497647.
STRING4932.YKR106W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR106W; YKR106W; YKR106W.
GeneID853981.
KEGGsce:YKR106W.

Organism-specific databases

CYGDYKR106w.
SGDS000001814. GEX2.

Phylogenomic databases

eggNOGNOG281153.
GeneTreeENSGT00390000002930.
OMANTSATLM.
OrthoDBEOG4HMNK4.

Gene expression databases

GenevestigatorP36173.
GermOnlineYKR106W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITEPS50850. MFS. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio975446.

Entry information

Entry nameGEX2_YEAST
AccessionPrimary (citable) accession number: P36173
Secondary accession number(s): D6VXG7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents