P36169 (SKG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Suppressor of lethality of KEX2 GAS1 double null mutant protein 1

Gene names

Name:	SKG1
Ordered Locus Names:	YKR100C/YKR099C-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in cell wall integrity. Affects the cell wall polymer composition in the growing region of the cell. Ref.1
Subcellular location	Cell membrane; Single-pass type III membrane protein; Cytoplasmic side. Bud membrane; Single-pass type III membrane protein; Cytoplasmic side. Note: Localizes on the inner surface of the plasma membrane at the bud and in the daughter cell. Localizes at an incipient bud site in the cells with emerging buds, a bud tip in small- or medium-budded cells, and a cell periphery in large-budded cells. Ref.1 Ref.5
Sequence similarities	Belongs to the SKG1 family.
Sequence caution	The sequence CAA82180.1 differs from that shown. Reason: Frameshift at position 234.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall organization Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	cellular bud membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud tip Inferred from direct assay Ref.1. Source: SGD incipient cellular bud site Inferred from direct assay Ref.1. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FMP45	Q07651	1	EBI-26550,EBI-2051056
GET3	Q12154	1	EBI-26550,EBI-2989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Suppressor of lethality of KEX2 GAS1 double null mutant protein 1		PRO_0000203230
Regions
	Topological domain	1 – 8	8	Extracellular Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type III membrane protein; Potential
	Topological domain	30 – 355	326	Cytoplasmic Potential
Amino acid modifications
	Modified residue	238	1	Phosphoserine Ref.7
	Modified residue	256	1	Phosphoserine Ref.6
	Modified residue	257	1	Phosphoserine Ref.6 Ref.7
	Modified residue	260	1	Phosphoserine Ref.6 Ref.7
	Modified residue	273	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P36169 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: D3BD732250D825FF	FASTA	355	39,830
	10 20 30 40 50 60 MTASTSVAVG CAVGIPVGVG IIIAVCFWFN LQKRYKREEQ DDRELERAIY DESGFVSFDN 70 80 90 100 110 120 FGPLRDSKDE AALASSELKN PDHTSGSSEG SAHPEEKDGK SRDQEKPLGK KNSKYYVPAY 130 140 150 160 170 180 RRKINLLQVR NNNYGNNARQ KSVVDLPSIN NSSNVSLSSS QRHITKRQIS VYDQMVPVIS 190 200 210 220 230 240 DEGPNFFADP SSDTNTSNDQ NKASMIELKH NTRQSSNENL IRNLQNQDFG SYYPRRASSS 250 260 270 280 290 300 FLNGNISNAS FHTRNSSITS VNKRDALEDV FATPKSAAQS QLPNTFDKDN EGMDADHSVK 310 320 330 340 350 DSRSAITDKD KDLYKLQNNY DVGNIGEIAE EDQYENEFTN YSQSKREFIE SLRPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition." Tomishige N., Noda Y., Adachi H., Shimoi H., Yoda K. Yeast 22:141-155(2005) [PubMed: 15645486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-266. Strain: ATCC 204511 / S288c / AB972.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-256; SER-257 AND SER-260, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238; SER-257; SER-260 AND THR-273, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28325 Genomic DNA. Translation: CAA82180.1. Frameshift. AY260883 Genomic DNA. Translation: AAP21751.1. BK006944 Genomic DNA. Translation: DAA09251.1.
PIR	S38179.
RefSeq	NP_013026.2. NM_001179890.1.
3D structure databases
ProteinModelPortal	P36169.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4605N.
IntAct	P36169. 2 interactions.
MINT	MINT-522252.
STRING	P36169.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR100C; YKR100C; YKR100C.
GeneID	853975.
KEGG	sce:YKR100C.
NMPDR	fig|4932.3.peg.4014.
Organism-specific databases
CYGD	YKR100c.
SGD	S000001808. SKG1.
Phylogenomic databases
eggNOG	fuNOG10973.
GeneTree	EFGT00050000008567.
HOGENOM	HBG202396.
OMA	EDQYENE.
OrthoDB	EOG4D2B0J.
Gene expression databases
ArrayExpress	P36169.
Genevestigator	P36169.
GermOnline	YKR100C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	975428.

Entry information

Entry name

SKG1_YEAST

Accession

Primary (citable) accession number: P36169
Secondary accession number(s): D6VXG1, Q86ZT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 31, 2006
Last modified:	December 14, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program