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P36165

- TGL4_YEAST

UniProt

P36165 - TGL4_YEAST

Protein

Lipase 4

Gene

TGL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation.1 Publication

    Catalytic activityi

    Triacylglycerol + H2O = diacylglycerol + a carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei315 – 3151By similarity

    GO - Molecular functioni

    1. calcium-independent phospholipase A2 activity Source: SGD
    2. lysophosphatidic acid acyltransferase activity Source: SGD
    3. sterol esterase activity Source: SGD
    4. triglyceride lipase activity Source: SGD

    GO - Biological processi

    1. cell budding Source: SGD
    2. phospholipid metabolic process Source: SGD
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
    4. triglyceride catabolic process Source: SGD
    5. triglyceride mobilization Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism, Sporulation

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-32052-MONOMER.
    YEAST:G3O-32052-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipase 4 (EC:3.1.1.3)
    Alternative name(s):
    Triacylglycerol lipase 4
    Gene namesi
    Name:TGL4
    Synonyms:STC1
    Ordered Locus Names:YKR089C
    ORF Names:YKR409
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR089c.
    SGDiS000001797. TGL4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. lipid particle Source: SGD

    Keywords - Cellular componenti

    Lipid droplet, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 910910Lipase 4PRO_0000203227Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551Phosphoserine1 Publication
    Modified residuei737 – 7371Phosphoserine1 Publication
    Modified residuei749 – 7491Phosphoserine1 Publication
    Modified residuei751 – 7511Phosphoserine2 Publications
    Modified residuei836 – 8361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36165.
    PaxDbiP36165.

    Expressioni

    Gene expression databases

    GenevestigatoriP36165.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACF2Q121681EBI-26531,EBI-32973
    OST1P415431EBI-26531,EBI-12651
    PMP3P872841EBI-26531,EBI-13555
    PPZ1P265701EBI-26531,EBI-13807
    PRP40P332031EBI-26531,EBI-701
    YHR080CP388001EBI-26531,EBI-24597

    Protein-protein interaction databases

    BioGridi34220. 36 interactions.
    DIPiDIP-5588N.
    IntActiP36165. 3 interactions.
    MINTiMINT-497371.
    STRINGi4932.YKR089C.

    Structurei

    3D structure databases

    ProteinModelPortaliP36165.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei282 – 30221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 32617HelicalSequence AnalysisAdd
    BLAST
    Transmembranei426 – 44217HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini282 – 483202PatatinAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi313 – 3175GXSXG

    Sequence similaritiesi

    Contains 1 patatin domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1752.
    GeneTreeiENSGT00390000016747.
    HOGENOMiHOG000065996.
    KOiK14674.
    OMAiPESHELI.
    OrthoDBiEOG7FR7QZ.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002641. Patatin/PLipase_A2-rel.
    IPR021771. Triacylglycerol_lipase.
    [Graphical view]
    PfamiPF11815. DUF3336. 1 hit.
    PF01734. Patatin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36165-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKISDLTS TQNKPLLVTQ QLIEKYYEQI LGTSQNIIPI LNPKNKFIRP    50
    SKDNSDVERV EEDAGKRLQT GKNKTTNKVN FNLDTGNEDK LDDDQETVTE 100
    NENNDIEMVE TDEGEDERQG SSLASKCKSF LYNVFVGNYE RDILIDKVCS 150
    QKQHAMSFEE WCSAGARLDD LTGKTEWKQK LESPLYDYKL IKDLTSRMRE 200
    ERLNRNYAQL LYIIRTNWVR NLGNMGNVNL YRHSHVGTKY LIDEYMMESR 250
    LALESLMESD LDDSYLLGIL QQTRRNIGRT ALVLSGGGTF GLFHIGVLGT 300
    LFELDLLPRV ISGSSAGAIV ASILSVHHKE EIPVLLNHIL DKEFNIFKDD 350
    KQKSESENLL IKISRFFKNG TWFDNKHLVN TMIEFLGDLT FREAYNRTGK 400
    ILNITVSPAS LFEQPRLLNN LTAPNVLIWS AVCASCSLPG IFPSSPLYEK 450
    DPKTGERKPW TGSSSVKFVD GSVDNDLPIS RLSEMFNVDH IIACQVNIHV 500
    FPFLKLSLSC VGGEIEDEFS ARLKQNLSSI YNFMANEAIH ILEIGSEMGI 550
    AKNALTKLRS VLSQQYSGDI TILPDMCMLF RIKELLSNPT KEFLLREITN 600
    GAKATWPKVS IIQNHCGQEF ALDKAISYIK GRMIVTSSLK TPFQFADSVI 650
    GLIKAPEQTS DESKNPENST LLTRTPTKGD NHISNVLDDN LLESESTNSL 700
    LLLRENASTY GRSPSGFRPR YSITSASLNP RHQRRKSDTI STSRRPAKSF 750
    SFSVASPTSR MLRQSSKING HPPPILQKKT SMGRLMFPMD AKTYDPESHE 800
    LIPHSASIET PAMVDKKLHF GRKSRYLRHM NKKWVSSSNI LYTDSDKEDH 850
    PTLRLISNFD SDAMIHSDLA GNFRRHSIDG RPPSQATKSS PFRSRPSSST 900
    QHKSTTSFTQ 910
    Length:910
    Mass (Da):102,717
    Last modified:June 1, 1994 - v1
    Checksum:i1CFC03C4A6E64B9C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z27116 Genomic DNA. Translation: CAA81640.1.
    Z28314 Genomic DNA. Translation: CAA82168.1.
    BK006944 Genomic DNA. Translation: DAA09239.1.
    PIRiS38167.
    RefSeqiNP_013015.1. NM_001179879.1.

    Genome annotation databases

    EnsemblFungiiYKR089C; YKR089C; YKR089C.
    GeneIDi853964.
    KEGGisce:YKR089C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z27116 Genomic DNA. Translation: CAA81640.1 .
    Z28314 Genomic DNA. Translation: CAA82168.1 .
    BK006944 Genomic DNA. Translation: DAA09239.1 .
    PIRi S38167.
    RefSeqi NP_013015.1. NM_001179879.1.

    3D structure databases

    ProteinModelPortali P36165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34220. 36 interactions.
    DIPi DIP-5588N.
    IntActi P36165. 3 interactions.
    MINTi MINT-497371.
    STRINGi 4932.YKR089C.

    Proteomic databases

    MaxQBi P36165.
    PaxDbi P36165.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR089C ; YKR089C ; YKR089C .
    GeneIDi 853964.
    KEGGi sce:YKR089C.

    Organism-specific databases

    CYGDi YKR089c.
    SGDi S000001797. TGL4.

    Phylogenomic databases

    eggNOGi COG1752.
    GeneTreei ENSGT00390000016747.
    HOGENOMi HOG000065996.
    KOi K14674.
    OMAi PESHELI.
    OrthoDBi EOG7FR7QZ.

    Enzyme and pathway databases

    BioCyci MetaCyc:G3O-32052-MONOMER.
    YEAST:G3O-32052-MONOMER.

    Miscellaneous databases

    NextBioi 975395.

    Gene expression databases

    Genevestigatori P36165.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002641. Patatin/PLipase_A2-rel.
    IPR021771. Triacylglycerol_lipase.
    [Graphical view ]
    Pfami PF11815. DUF3336. 1 hit.
    PF01734. Patatin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
      Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
      Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles."
      Athenstaedt K., Daum G.
      J. Biol. Chem. 280:37301-37309(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-751, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-749; SER-751 AND SER-836, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTGL4_YEAST
    AccessioniPrimary (citable) accession number: P36165
    Secondary accession number(s): D6VXE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 195 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3