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P36165

- TGL4_YEAST

UniProt

P36165 - TGL4_YEAST

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Protein

Lipase 4

Gene
TGL4, STC1, YKR089C, YKR409
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation.1 Publication

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei315 – 3151 By similarity

GO - Molecular functioni

  1. calcium-independent phospholipase A2 activity Source: SGD
  2. lysophosphatidic acid acyltransferase activity Source: SGD
  3. sterol esterase activity Source: SGD
  4. triglyceride lipase activity Source: SGD

GO - Biological processi

  1. cell budding Source: SGD
  2. phospholipid metabolic process Source: SGD
  3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  4. triglyceride catabolic process Source: SGD
  5. triglyceride mobilization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32052-MONOMER.
YEAST:G3O-32052-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 4 (EC:3.1.1.3)
Alternative name(s):
Triacylglycerol lipase 4
Gene namesi
Name:TGL4
Synonyms:STC1
Ordered Locus Names:YKR089C
ORF Names:YKR409
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR089c.
SGDiS000001797. TGL4.

Subcellular locationi

Lipid droplet. Membrane; Multi-pass membrane protein
Note: Lipid particle.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei282 – 30221Helical; Reviewed predictionAdd
BLAST
Transmembranei310 – 32617Helical; Reviewed predictionAdd
BLAST
Transmembranei426 – 44217Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 910910Lipase 4PRO_0000203227Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine1 Publication
Modified residuei737 – 7371Phosphoserine1 Publication
Modified residuei749 – 7491Phosphoserine1 Publication
Modified residuei751 – 7511Phosphoserine2 Publications
Modified residuei836 – 8361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36165.
PaxDbiP36165.

Expressioni

Gene expression databases

GenevestigatoriP36165.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ACF2Q121681EBI-26531,EBI-32973
OST1P415431EBI-26531,EBI-12651
PMP3P872841EBI-26531,EBI-13555
PPZ1P265701EBI-26531,EBI-13807
PRP40P332031EBI-26531,EBI-701
YHR080CP388001EBI-26531,EBI-24597

Protein-protein interaction databases

BioGridi34220. 36 interactions.
DIPiDIP-5588N.
IntActiP36165. 3 interactions.
MINTiMINT-497371.
STRINGi4932.YKR089C.

Structurei

3D structure databases

ProteinModelPortaliP36165.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini282 – 483202PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi313 – 3175GXSXG

Sequence similaritiesi

Contains 1 patatin domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1752.
GeneTreeiENSGT00390000016747.
HOGENOMiHOG000065996.
KOiK14674.
OMAiPESHELI.
OrthoDBiEOG7FR7QZ.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
IPR021771. Triacylglycerol_lipase.
[Graphical view]
PfamiPF11815. DUF3336. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.

Sequencei

Sequence statusi: Complete.

P36165-1 [UniParc]FASTAAdd to Basket

« Hide

MSSKISDLTS TQNKPLLVTQ QLIEKYYEQI LGTSQNIIPI LNPKNKFIRP    50
SKDNSDVERV EEDAGKRLQT GKNKTTNKVN FNLDTGNEDK LDDDQETVTE 100
NENNDIEMVE TDEGEDERQG SSLASKCKSF LYNVFVGNYE RDILIDKVCS 150
QKQHAMSFEE WCSAGARLDD LTGKTEWKQK LESPLYDYKL IKDLTSRMRE 200
ERLNRNYAQL LYIIRTNWVR NLGNMGNVNL YRHSHVGTKY LIDEYMMESR 250
LALESLMESD LDDSYLLGIL QQTRRNIGRT ALVLSGGGTF GLFHIGVLGT 300
LFELDLLPRV ISGSSAGAIV ASILSVHHKE EIPVLLNHIL DKEFNIFKDD 350
KQKSESENLL IKISRFFKNG TWFDNKHLVN TMIEFLGDLT FREAYNRTGK 400
ILNITVSPAS LFEQPRLLNN LTAPNVLIWS AVCASCSLPG IFPSSPLYEK 450
DPKTGERKPW TGSSSVKFVD GSVDNDLPIS RLSEMFNVDH IIACQVNIHV 500
FPFLKLSLSC VGGEIEDEFS ARLKQNLSSI YNFMANEAIH ILEIGSEMGI 550
AKNALTKLRS VLSQQYSGDI TILPDMCMLF RIKELLSNPT KEFLLREITN 600
GAKATWPKVS IIQNHCGQEF ALDKAISYIK GRMIVTSSLK TPFQFADSVI 650
GLIKAPEQTS DESKNPENST LLTRTPTKGD NHISNVLDDN LLESESTNSL 700
LLLRENASTY GRSPSGFRPR YSITSASLNP RHQRRKSDTI STSRRPAKSF 750
SFSVASPTSR MLRQSSKING HPPPILQKKT SMGRLMFPMD AKTYDPESHE 800
LIPHSASIET PAMVDKKLHF GRKSRYLRHM NKKWVSSSNI LYTDSDKEDH 850
PTLRLISNFD SDAMIHSDLA GNFRRHSIDG RPPSQATKSS PFRSRPSSST 900
QHKSTTSFTQ 910
Length:910
Mass (Da):102,717
Last modified:June 1, 1994 - v1
Checksum:i1CFC03C4A6E64B9C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z27116 Genomic DNA. Translation: CAA81640.1.
Z28314 Genomic DNA. Translation: CAA82168.1.
BK006944 Genomic DNA. Translation: DAA09239.1.
PIRiS38167.
RefSeqiNP_013015.1. NM_001179879.1.

Genome annotation databases

EnsemblFungiiYKR089C; YKR089C; YKR089C.
GeneIDi853964.
KEGGisce:YKR089C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z27116 Genomic DNA. Translation: CAA81640.1 .
Z28314 Genomic DNA. Translation: CAA82168.1 .
BK006944 Genomic DNA. Translation: DAA09239.1 .
PIRi S38167.
RefSeqi NP_013015.1. NM_001179879.1.

3D structure databases

ProteinModelPortali P36165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34220. 36 interactions.
DIPi DIP-5588N.
IntActi P36165. 3 interactions.
MINTi MINT-497371.
STRINGi 4932.YKR089C.

Proteomic databases

MaxQBi P36165.
PaxDbi P36165.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR089C ; YKR089C ; YKR089C .
GeneIDi 853964.
KEGGi sce:YKR089C.

Organism-specific databases

CYGDi YKR089c.
SGDi S000001797. TGL4.

Phylogenomic databases

eggNOGi COG1752.
GeneTreei ENSGT00390000016747.
HOGENOMi HOG000065996.
KOi K14674.
OMAi PESHELI.
OrthoDBi EOG7FR7QZ.

Enzyme and pathway databases

BioCyci MetaCyc:G3O-32052-MONOMER.
YEAST:G3O-32052-MONOMER.

Miscellaneous databases

NextBioi 975395.

Gene expression databases

Genevestigatori P36165.

Family and domain databases

InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
IPR021771. Triacylglycerol_lipase.
[Graphical view ]
Pfami PF11815. DUF3336. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
    Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
    Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles."
    Athenstaedt K., Daum G.
    J. Biol. Chem. 280:37301-37309(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-751, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-749; SER-751 AND SER-836, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTGL4_YEAST
AccessioniPrimary (citable) accession number: P36165
Secondary accession number(s): D6VXE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi