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Protein

Lipase 4

Gene

TGL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation.1 Publication

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei315NucleophilePROSITE-ProRule annotation1
Active sitei470Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • calcium-independent phospholipase A2 activity Source: SGD
  • lysophosphatidic acid acyltransferase activity Source: SGD
  • sterol esterase activity Source: SGD
  • triglyceride lipase activity Source: SGD

GO - Biological processi

  • cell budding Source: SGD
  • phospholipid metabolic process Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • triglyceride catabolic process Source: SGD
  • triglyceride mobilization Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Sporulation

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32052-MONOMER
YEAST:G3O-32052-MONOMER

Chemistry databases

SwissLipidsiSLP:000000053
SLP:000000672

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 4 (EC:3.1.1.3)
Alternative name(s):
Triacylglycerol lipase 4
Gene namesi
Name:TGL4
Synonyms:STC1
Ordered Locus Names:YKR089C
ORF Names:YKR409
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR089C
SGDiS000001797 TGL4

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Transmembranei310 – 326HelicalSequence analysisAdd BLAST17
Transmembranei426 – 442HelicalSequence analysisAdd BLAST17

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002032271 – 910Lipase 4Add BLAST910

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineCombined sources1
Modified residuei737PhosphoserineCombined sources1
Modified residuei749PhosphoserineCombined sources1
Modified residuei751PhosphoserineCombined sources1
Modified residuei836PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36165
PaxDbiP36165
PRIDEiP36165

PTM databases

iPTMnetiP36165

Interactioni

Protein-protein interaction databases

BioGridi34220, 57 interactors
DIPiDIP-5588N
IntActiP36165, 3 interactors
MINTiP36165
STRINGi4932.YKR089C

Structurei

3D structure databases

ProteinModelPortaliP36165
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini282 – 483PNPLAPROSITE-ProRule annotationAdd BLAST202

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi286 – 291GXGXXGPROSITE-ProRule annotation6
Motifi313 – 317GXSXGPROSITE-ProRule annotation5

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016747
HOGENOMiHOG000065996
InParanoidiP36165
KOiK14674
OMAiNFMANEA
OrthoDBiEOG092C24S4

Family and domain databases

InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR002641 PNPLA_dom
IPR021771 Triacylglycerol_lipase
PfamiView protein in Pfam
PF11815 DUF3336, 1 hit
PF01734 Patatin, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS51635 PNPLA, 1 hit

Sequencei

Sequence statusi: Complete.

P36165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKISDLTS TQNKPLLVTQ QLIEKYYEQI LGTSQNIIPI LNPKNKFIRP
60 70 80 90 100
SKDNSDVERV EEDAGKRLQT GKNKTTNKVN FNLDTGNEDK LDDDQETVTE
110 120 130 140 150
NENNDIEMVE TDEGEDERQG SSLASKCKSF LYNVFVGNYE RDILIDKVCS
160 170 180 190 200
QKQHAMSFEE WCSAGARLDD LTGKTEWKQK LESPLYDYKL IKDLTSRMRE
210 220 230 240 250
ERLNRNYAQL LYIIRTNWVR NLGNMGNVNL YRHSHVGTKY LIDEYMMESR
260 270 280 290 300
LALESLMESD LDDSYLLGIL QQTRRNIGRT ALVLSGGGTF GLFHIGVLGT
310 320 330 340 350
LFELDLLPRV ISGSSAGAIV ASILSVHHKE EIPVLLNHIL DKEFNIFKDD
360 370 380 390 400
KQKSESENLL IKISRFFKNG TWFDNKHLVN TMIEFLGDLT FREAYNRTGK
410 420 430 440 450
ILNITVSPAS LFEQPRLLNN LTAPNVLIWS AVCASCSLPG IFPSSPLYEK
460 470 480 490 500
DPKTGERKPW TGSSSVKFVD GSVDNDLPIS RLSEMFNVDH IIACQVNIHV
510 520 530 540 550
FPFLKLSLSC VGGEIEDEFS ARLKQNLSSI YNFMANEAIH ILEIGSEMGI
560 570 580 590 600
AKNALTKLRS VLSQQYSGDI TILPDMCMLF RIKELLSNPT KEFLLREITN
610 620 630 640 650
GAKATWPKVS IIQNHCGQEF ALDKAISYIK GRMIVTSSLK TPFQFADSVI
660 670 680 690 700
GLIKAPEQTS DESKNPENST LLTRTPTKGD NHISNVLDDN LLESESTNSL
710 720 730 740 750
LLLRENASTY GRSPSGFRPR YSITSASLNP RHQRRKSDTI STSRRPAKSF
760 770 780 790 800
SFSVASPTSR MLRQSSKING HPPPILQKKT SMGRLMFPMD AKTYDPESHE
810 820 830 840 850
LIPHSASIET PAMVDKKLHF GRKSRYLRHM NKKWVSSSNI LYTDSDKEDH
860 870 880 890 900
PTLRLISNFD SDAMIHSDLA GNFRRHSIDG RPPSQATKSS PFRSRPSSST
910
QHKSTTSFTQ
Length:910
Mass (Da):102,717
Last modified:June 1, 1994 - v1
Checksum:i1CFC03C4A6E64B9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27116 Genomic DNA Translation: CAA81640.1
Z28314 Genomic DNA Translation: CAA82168.1
BK006944 Genomic DNA Translation: DAA09239.1
PIRiS38167
RefSeqiNP_013015.1, NM_001179879.1

Genome annotation databases

EnsemblFungiiYKR089C; YKR089C; YKR089C
GeneIDi853964
KEGGisce:YKR089C

Similar proteinsi

Entry informationi

Entry nameiTGL4_YEAST
AccessioniPrimary (citable) accession number: P36165
Secondary accession number(s): D6VXE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health