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P36165 (TGL4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipase 4

EC=3.1.1.3
Alternative name(s):
Triacylglycerol lipase 4
Gene names
Name:TGL4
Synonyms:STC1
Ordered Locus Names:YKR089C
ORF Names:YKR409
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length910 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation. Ref.6

Catalytic activity

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Subcellular location

Lipid droplet. Membrane; Multi-pass membrane protein. Note: Lipid particle. Ref.4 Ref.6

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 patatin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 910910Lipase 4
PRO_0000203227

Regions

Transmembrane282 – 30221Helical; Potential
Transmembrane310 – 32617Helical; Potential
Transmembrane426 – 44217Helical; Potential
Domain282 – 483202Patatin
Motif313 – 3175GXSXG

Sites

Active site3151 By similarity

Amino acid modifications

Modified residue551Phosphoserine Ref.10
Modified residue7371Phosphoserine Ref.9
Modified residue7491Phosphoserine Ref.10
Modified residue7511Phosphoserine Ref.7 Ref.10
Modified residue8361Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P36165 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 1CFC03C4A6E64B9C

FASTA910102,717
        10         20         30         40         50         60 
MSSKISDLTS TQNKPLLVTQ QLIEKYYEQI LGTSQNIIPI LNPKNKFIRP SKDNSDVERV 

        70         80         90        100        110        120 
EEDAGKRLQT GKNKTTNKVN FNLDTGNEDK LDDDQETVTE NENNDIEMVE TDEGEDERQG 

       130        140        150        160        170        180 
SSLASKCKSF LYNVFVGNYE RDILIDKVCS QKQHAMSFEE WCSAGARLDD LTGKTEWKQK 

       190        200        210        220        230        240 
LESPLYDYKL IKDLTSRMRE ERLNRNYAQL LYIIRTNWVR NLGNMGNVNL YRHSHVGTKY 

       250        260        270        280        290        300 
LIDEYMMESR LALESLMESD LDDSYLLGIL QQTRRNIGRT ALVLSGGGTF GLFHIGVLGT 

       310        320        330        340        350        360 
LFELDLLPRV ISGSSAGAIV ASILSVHHKE EIPVLLNHIL DKEFNIFKDD KQKSESENLL 

       370        380        390        400        410        420 
IKISRFFKNG TWFDNKHLVN TMIEFLGDLT FREAYNRTGK ILNITVSPAS LFEQPRLLNN 

       430        440        450        460        470        480 
LTAPNVLIWS AVCASCSLPG IFPSSPLYEK DPKTGERKPW TGSSSVKFVD GSVDNDLPIS 

       490        500        510        520        530        540 
RLSEMFNVDH IIACQVNIHV FPFLKLSLSC VGGEIEDEFS ARLKQNLSSI YNFMANEAIH 

       550        560        570        580        590        600 
ILEIGSEMGI AKNALTKLRS VLSQQYSGDI TILPDMCMLF RIKELLSNPT KEFLLREITN 

       610        620        630        640        650        660 
GAKATWPKVS IIQNHCGQEF ALDKAISYIK GRMIVTSSLK TPFQFADSVI GLIKAPEQTS 

       670        680        690        700        710        720 
DESKNPENST LLTRTPTKGD NHISNVLDDN LLESESTNSL LLLRENASTY GRSPSGFRPR 

       730        740        750        760        770        780 
YSITSASLNP RHQRRKSDTI STSRRPAKSF SFSVASPTSR MLRQSSKING HPPPILQKKT 

       790        800        810        820        830        840 
SMGRLMFPMD AKTYDPESHE LIPHSASIET PAMVDKKLHF GRKSRYLRHM NKKWVSSSNI 

       850        860        870        880        890        900 
LYTDSDKEDH PTLRLISNFD SDAMIHSDLA GNFRRHSIDG RPPSQATKSS PFRSRPSSST 

       910 
QHKSTTSFTQ 

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles."
Athenstaedt K., Daum G.
J. Biol. Chem. 280:37301-37309(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-751, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-749; SER-751 AND SER-836, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z27116 Genomic DNA. Translation: CAA81640.1.
Z28314 Genomic DNA. Translation: CAA82168.1.
BK006944 Genomic DNA. Translation: DAA09239.1.
PIRS38167.
RefSeqNP_013015.1. NM_001179879.1.

3D structure databases

ProteinModelPortalP36165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34220. 36 interactions.
DIPDIP-5588N.
IntActP36165. 3 interactions.
MINTMINT-497371.
STRING4932.YKR089C.

Proteomic databases

PaxDbP36165.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR089C; YKR089C; YKR089C.
GeneID853964.
KEGGsce:YKR089C.

Organism-specific databases

CYGDYKR089c.
SGDS000001797. TGL4.

Phylogenomic databases

eggNOGCOG1752.
GeneTreeENSGT00390000016747.
HOGENOMHOG000065996.
KOK14674.
OrthoDBEOG7FR7QZ.

Enzyme and pathway databases

BioCycMetaCyc:G3O-32052-MONOMER.
YEAST:G3O-32052-MONOMER.

Gene expression databases

GenevestigatorP36165.

Family and domain databases

InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
IPR021771. Triacylglycerol_lipase.
[Graphical view]
PfamPF11815. DUF3336. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMSSF52151. SSF52151. 1 hit.
ProtoNetSearch...

Other

NextBio975395.

Entry information

Entry nameTGL4_YEAST
AccessionPrimary (citable) accession number: P36165
Secondary accession number(s): D6VXE9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 19, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families