Lipase 4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P36165 (TGL4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Lipase 4
EC=3.1.1.3

Alternative name(s):
Triacylglycerol lipase 4

Gene names

Name:	TGL4
Synonyms:	STC1
Ordered Locus Names:	YKR089C
ORF Names:	YKR409

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process. May have a specific role in sporulation. Ref.6
Catalytic activity	Triacylglycerol + H₂O = diacylglycerol + a carboxylate.
Subcellular location	Lipid droplet. Membrane; Multi-pass membrane protein. Note: Lipid particle. Ref.4 Ref.6
Miscellaneous	Present with 195 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 patatin domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism Sporulation
Cellular component	Lipid droplet Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell budding Inferred from mutant phenotype PubMed 19150427. Source: SGD phospholipid metabolic process Inferred from mutant phenotype PubMed 20332534. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW triglyceride catabolic process Inferred from mutant phenotype PubMed 16267052. Source: SGD triglyceride mobilization Inferred from direct assay Ref.6. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lipid particle Inferred from direct assay Ref.4 Ref.6. Source: SGD
Molecular_function	calcium-independent phospholipase A2 activity Inferred from direct assay PubMed 20332534. Source: SGD lysophosphatidic acid acyltransferase activity Inferred from direct assay PubMed 20332534. Source: SGD retinyl-palmitate esterase activity Inferred from electronic annotation. Source: EC sterol esterase activity Inferred from direct assay PubMed 20332534. Source: SGD triglyceride lipase activity Inferred from direct assay Ref.6 PubMed 20332534. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ACF2	Q12168	1	EBI-26531,EBI-32973
OST1	P41543	1	EBI-26531,EBI-12651
PMP3	P87284	1	EBI-26531,EBI-13555
PPZ1	P26570	1	EBI-26531,EBI-13807
PRP40	P33203	1	EBI-26531,EBI-701
YHR080C	P38800	1	EBI-26531,EBI-24597

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 910

910

Lipase 4

PRO_0000203227

Regions

Transmembrane

282 – 302

Helical; Potential

Transmembrane

310 – 326

Helical; Potential

Transmembrane

426 – 442

Helical; Potential

Domain

282 – 483

202

Patatin

Motif

313 – 317

GXSXG

Sites

Active site

315

By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

675

Phosphothreonine Ref.10

Modified residue

737

Phosphoserine Ref.9 Ref.10

Modified residue

751

Phosphoserine Ref.7 Ref.10

Modified residue

756

Phosphoserine Ref.10

Modified residue

781

Phosphoserine Ref.8

Modified residue

836

Phosphoserine Ref.10

Modified residue

890

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P36165 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 1CFC03C4A6E64B9C
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FASTA

910

102,717

        10         20         30         40         50         60 
MSSKISDLTS TQNKPLLVTQ QLIEKYYEQI LGTSQNIIPI LNPKNKFIRP SKDNSDVERV 

        70         80         90        100        110        120 
EEDAGKRLQT GKNKTTNKVN FNLDTGNEDK LDDDQETVTE NENNDIEMVE TDEGEDERQG 

       130        140        150        160        170        180 
SSLASKCKSF LYNVFVGNYE RDILIDKVCS QKQHAMSFEE WCSAGARLDD LTGKTEWKQK 

       190        200        210        220        230        240 
LESPLYDYKL IKDLTSRMRE ERLNRNYAQL LYIIRTNWVR NLGNMGNVNL YRHSHVGTKY 

       250        260        270        280        290        300 
LIDEYMMESR LALESLMESD LDDSYLLGIL QQTRRNIGRT ALVLSGGGTF GLFHIGVLGT 

       310        320        330        340        350        360 
LFELDLLPRV ISGSSAGAIV ASILSVHHKE EIPVLLNHIL DKEFNIFKDD KQKSESENLL 

       370        380        390        400        410        420 
IKISRFFKNG TWFDNKHLVN TMIEFLGDLT FREAYNRTGK ILNITVSPAS LFEQPRLLNN 

       430        440        450        460        470        480 
LTAPNVLIWS AVCASCSLPG IFPSSPLYEK DPKTGERKPW TGSSSVKFVD GSVDNDLPIS 

       490        500        510        520        530        540 
RLSEMFNVDH IIACQVNIHV FPFLKLSLSC VGGEIEDEFS ARLKQNLSSI YNFMANEAIH 

       550        560        570        580        590        600 
ILEIGSEMGI AKNALTKLRS VLSQQYSGDI TILPDMCMLF RIKELLSNPT KEFLLREITN 

       610        620        630        640        650        660 
GAKATWPKVS IIQNHCGQEF ALDKAISYIK GRMIVTSSLK TPFQFADSVI GLIKAPEQTS 

       670        680        690        700        710        720 
DESKNPENST LLTRTPTKGD NHISNVLDDN LLESESTNSL LLLRENASTY GRSPSGFRPR 

       730        740        750        760        770        780 
YSITSASLNP RHQRRKSDTI STSRRPAKSF SFSVASPTSR MLRQSSKING HPPPILQKKT 

       790        800        810        820        830        840 
SMGRLMFPMD AKTYDPESHE LIPHSASIET PAMVDKKLHF GRKSRYLRHM NKKWVSSSNI 

       850        860        870        880        890        900 
LYTDSDKEDH PTLRLISNFD SDAMIHSDLA GNFRRHSIDG RPPSQATKSS PFRSRPSSST 

       910 
QHKSTTSFTQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames." Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F. Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles." Athenstaedt K., Daum G. J. Biol. Chem. 280:37301-37309(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-751, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-781 AND SER-890, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; THR-675; SER-737; SER-751; SER-756 AND SER-836, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z27116 Genomic DNA. Translation: CAA81640.1. Z28314 Genomic DNA. Translation: CAA82168.1. BK006944 Genomic DNA. Translation: DAA09239.1.
PIR	S38167.
RefSeq	NP_013015.1. NM_001179879.1.
3D structure databases
ProteinModelPortal	P36165.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5588N.
IntAct	P36165. 2 interactions.
MINT	MINT-497371.
STRING	4932.YKR089C.
Proteomic databases
PaxDb	P36165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR089C; YKR089C; YKR089C.
GeneID	853964.
KEGG	sce:YKR089C.
Organism-specific databases
CYGD	YKR089c.
SGD	S000001797. TGL4.
Phylogenomic databases
eggNOG	COG1752.
GeneTree	ENSGT00390000016747.
HOGENOM	HOG000065996.
KO	K14674.
OrthoDB	EOG4BZR9Q.
Gene expression databases
Genevestigator	P36165.
GermOnline	YKR089C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016035. Acyl_Trfase/lysoPLipase. IPR002641. Patatin/PLipase_A2-rel. IPR021771. Triacylglycerol_lipase. [Graphical view]
Pfam	PF11815. DUF3336. 1 hit. PF01734. Patatin. 1 hit. [Graphical view]
SUPFAM	SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
ProtoNet	Search...
Other
NextBio	975395.

Entry information

Entry name

TGL4_YEAST

Accession

Primary (citable) accession number: P36165
Secondary accession number(s): D6VXE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents