Reviewed, UniProtKB/Swiss-Prot P36164 (TVP38_YEAST)

Names and origin

Protein names

Recommended name:
    Golgi apparatus membrane protein TVP38
Alternative name(s):
    TLG2-vesicle protein of 38 kDa

Gene names

Name:	TVP38
Ordered Locus Names:	YKR088C
ORF Names:	YKR408

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Golgi membrane protein involved in vesicular trafficking and spindle migration. Ref.8
Subunit structure	Interacts with YIP5. Ref.8
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Ref.8 Ref.4 Ref.6
Miscellaneous	Present with 11300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the TVP38/TMEM64 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitotic spindle elongation Inferred from mutant phenotype. Source: SGD vesicle-mediated transport Ref.8 Inferred from genetic interaction. Source: SGD
Cellular component	integral to Golgi membrane Ref.6 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AKR1	P39010	1	EBI-26527,EBI-2421
GCS1	P35197	1	EBI-26527,EBI-7475
KEX1	P09620	1	EBI-26527,EBI-9653
RBD2	Q12270	1	EBI-26527,EBI-31471
TEF1	P02994	1	EBI-26527,EBI-6314
TVP15	Q03860	1	EBI-26527,EBI-37537

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Golgi apparatus membrane protein TVP38		PRO_0000203226
Regions
	Topological domain	1 – 95	95	Lumenal Potential
	Transmembrane	96 – 116	21	Potential
	Topological domain	117 – 137	21	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Potential
	Topological domain	159 – 169	11	Lumenal Potential
	Transmembrane	170 – 190	21	Potential
	Topological domain	191 – 251	61	Cytoplasmic Potential
	Transmembrane	252 – 272	21	Potential
	Topological domain	273 – 286	14	Lumenal Potential
	Transmembrane	287 – 307	21	Potential
	Topological domain	308 – 337	30	Cytoplasmic Potential
Amino acid modifications
	Modified residue	326	1	Phosphoserine Ref.9
	Modified residue	327	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P36164-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 7EA95DD4E5AF77FE	FASTA	337	38,311
	10 20 30 40 50 60 MSQSYEAGNA NMGQGEDDEF DGYFEDFDND IMPNSNNGQR VGTNAGLSFN DEVNVNDDDF 70 80 90 100 110 120 LDIYNMSPRE RLMHNIRKNV QKLQFYFYSL RLWQQIIIVL LGIMLMIMGI LLLVFHNAIL 130 140 150 160 170 180 HKVVVTSNDL REKMSTHFIL MVLIFFVAFP PMIGYSLLST TTGLIYGVSF EGWVTLALGS 190 200 210 220 230 240 VTGSIASFVV FKTILHSRAE KLVHLNRRFE ALASILQENN SYWILALLRL CPFPYSLTNG 250 260 270 280 290 300 AIAGVYGISV RNFSIANIIT TPKLFIYLFI GSRVKSLAES ESTGSRVFDL VSIIITLLIL 310 320 330 SLTAWLLYFK TKKRYLELQN RDRQVSTDQL PELSFEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames." Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F. Yeast 10:231-245(1994) [PubMed: 8203164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed: 16107716] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[8]	"Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae." Inadome H., Noda Y., Kamimura Y., Adachi H., Yoda K. Exp. Cell Res. 313:688-697(2007) [PubMed: 17178117] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH YIP5.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326 AND THR-327, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z27116 Genomic DNA. Translation: CAA81639.1. Z28313 Genomic DNA. Translation: CAA82167.1. AY557916 Genomic DNA. Translation: AAS56242.1.
PIR	S38166.
RefSeq	NP_013014.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5167N.
IntAct	P36164. 7 interactions.
STRING	P36164.
Genome annotation databases
Ensembl	YKR088C; YKR088C; YKR088C; Saccharomyces cerevisiae. [Genome view]
GeneID	853963.
GenomeReviews	Gene locus YKR088C in contig Y13137_GR.
KEGG	sce:YKR088C.
NMPDR	fig|4932.3.peg.4001.
Organism-specific databases
CYGD	YKR088c.
SGD	S000001796. TVP38.
Phylogenomic databases
HOGENOM	P36164.
OMA	LCPFPYS.
Gene expression databases
ArrayExpress	P36164.
Genevestigator	P36164.
GermOnline	YKR088C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015414. SNARE_assoc. [Graphical view]
Pfam	PF09335. SNARE_assoc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975392.

Entry information

Entry name

TVP38_YEAST

Accession

Primary (citable) accession number: P36164

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)