P36164 (TVP38_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi apparatus membrane protein TVP38

Alternative name(s):
TLG2-vesicle protein of 38 kDa

Gene names

Name:	TVP38
Ordered Locus Names:	YKR088C
ORF Names:	YKR408

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Golgi membrane protein involved in vesicular trafficking and spindle migration. Ref.9
Subunit structure	Interacts with YIP5. Ref.9
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein Ref.5 Ref.7 Ref.9.
Miscellaneous	Present with 11300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TVP38/TMEM64 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitotic spindle elongation Inferred from mutant phenotype PubMed 18194531. Source: SGD vesicle-mediated transport Inferred from genetic interaction Ref.9. Source: SGD
Cellular_component	integral to Golgi membrane Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AKR1	P39010	1	EBI-26527,EBI-2421
GCS1	P35197	1	EBI-26527,EBI-7475
KEX1	P09620	1	EBI-26527,EBI-9653
RBD2	Q12270	1	EBI-26527,EBI-31471
TEF1	P02994	1	EBI-26527,EBI-6314
TVP15	Q03860	1	EBI-26527,EBI-37537

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Golgi apparatus membrane protein TVP38		PRO_0000203226
Regions
	Topological domain	1 – 95	95	Lumenal Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Topological domain	117 – 137	21	Cytoplasmic Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Topological domain	159 – 169	11	Lumenal Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Topological domain	191 – 251	61	Cytoplasmic Potential
	Transmembrane	252 – 272	21	Helical; Potential
	Topological domain	273 – 286	14	Lumenal Potential
	Transmembrane	287 – 307	21	Helical; Potential
	Topological domain	308 – 337	30	Cytoplasmic Potential
Amino acid modifications
	Modified residue	326	1	Phosphoserine Ref.10
	Modified residue	327	1	Phosphothreonine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P36164 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 7EA95DD4E5AF77FE	FASTA	337	38,311
	10 20 30 40 50 60 MSQSYEAGNA NMGQGEDDEF DGYFEDFDND IMPNSNNGQR VGTNAGLSFN DEVNVNDDDF 70 80 90 100 110 120 LDIYNMSPRE RLMHNIRKNV QKLQFYFYSL RLWQQIIIVL LGIMLMIMGI LLLVFHNAIL 130 140 150 160 170 180 HKVVVTSNDL REKMSTHFIL MVLIFFVAFP PMIGYSLLST TTGLIYGVSF EGWVTLALGS 190 200 210 220 230 240 VTGSIASFVV FKTILHSRAE KLVHLNRRFE ALASILQENN SYWILALLRL CPFPYSLTNG 250 260 270 280 290 300 AIAGVYGISV RNFSIANIIT TPKLFIYLFI GSRVKSLAES ESTGSRVFDL VSIIITLLIL 310 320 330 SLTAWLLYFK TKKRYLELQN RDRQVSTDQL PELSFEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames." Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F. Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[9]	"Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae." Inadome H., Noda Y., Kamimura Y., Adachi H., Yoda K. Exp. Cell Res. 313:688-697(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH YIP5.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326 AND THR-327, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z27116 Genomic DNA. Translation: CAA81639.1. Z28313 Genomic DNA. Translation: CAA82167.1. AY557916 Genomic DNA. Translation: AAS56242.1. BK006944 Genomic DNA. Translation: DAA09238.1.
PIR	S38166.
RefSeq	NP_013014.3. NM_001179878.3.
3D structure databases
ProteinModelPortal	P36164.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5167N.
IntAct	P36164. 6 interactions.
MINT	MINT-563946.
STRING	4932.YKR088C.
Proteomic databases
PaxDb	P36164.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR088C; YKR088C; YKR088C.
GeneID	853963.
KEGG	sce:YKR088C. sce:YKR090W.
Organism-specific databases
CYGD	YKR088c.
SGD	S000001796. TVP38.
Phylogenomic databases
eggNOG	COG0398.
HOGENOM	HOG000066098.
OMA	RIGFNDF.
OrthoDB	EOG4P2TB6.
Gene expression databases
Genevestigator	P36164.
GermOnline	YKR088C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015414. SNARE_assoc. [Graphical view]
Pfam	PF09335. SNARE_assoc. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975392.

Entry information

Entry name

TVP38_YEAST

Accession

Primary (citable) accession number: P36164
Secondary accession number(s): D6VXE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program