P36163 (OMA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial metalloendopeptidase OMA1
EC=3.4.24.-

Gene names

Name:	OMA1
Ordered Locus Names:	YKR087C
ORF Names:	YKR407

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protease that is part of the quality control system in the inner membrane of mitochondria. Cleaves and thereby promotes the turnover of mistranslated or misfolded membrane proteins. Can cleave the misfolded multi-pass membrane protein OXA1. Ref.4
Cofactor	Binds 1 zinc ion per subunit Potential.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.4 Ref.6.
Sequence similarities	Belongs to the peptidase M48 family.
Sequence caution	The sequence CAA81638.1 differs from that shown. Reason: Frameshift at position 33. The sequence CAA82166.1 differs from that shown. Reason: Frameshift at position 33.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	misfolded or incompletely synthesized protein catabolic process Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay Ref.4. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Mitochondrial metalloendopeptidase OMA1		PRO_0000203225
Regions
	Topological domain	1 – 67	67	Mitochondrial intermembrane Potential
	Transmembrane	68 – 88	21	Helical; Potential
	Topological domain	89 – 223	135	Mitochondrial matrix Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Topological domain	245 – 345	101	Mitochondrial intermembrane Potential
Sites
	Active site	204	1	By similarity
	Metal binding	203	1	Zinc; catalytic By similarity
	Metal binding	207	1	Zinc; catalytic By similarity
	Metal binding	257	1	Zinc; catalytic By similarity
Experimental info
	Mutagenesis	204	1	E → Q: Loss of protease activity. Ref.4
	Mutagenesis	207	1	H → Y: Loss of protease activity. Ref.4
	Mutagenesis	212	1	H → Y: Loss of protease activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36163 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 95B0EBCD25F435CF	FASTA	345	39,328
	10 20 30 40 50 60 MLRNIIRFKG FGKGTSGGFL KPVSFRVQLT RCYRYDNGPS YRRFNNGEYS QKSSFKSILL 70 80 90 100 110 120 DKSSRKYLAL LFGGCSLFYY THLDKAPVSD RSRFIWVSRP LELTIGNYTY KSIWRQTQQE 130 140 150 160 170 180 ILPPQHPLSI KIENIFMKIV EAAYKDPSVD NSLLDGIKWE IHVVNDPTAS PNAFVLPGGK 190 200 210 220 230 240 VFIFSSILPI CANDDGIATV LAHEFAHQLA RHTAENLSKA PIYSLLGLVL YTVTGAHAIN 250 260 270 280 290 300 NILLDGFLRM PASRQMETEA DYIGLMIMSR ACFQPQESIK VWERMANFEK QMNRGGVVNM 310 320 330 340 EFLSTHPAST RRIENMSKWL PKANEIYEQS DCSSMGNYYK SFFSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames." Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F. Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Oma1, a novel membrane-bound metallopeptidase in mitochondria with activities overlapping with the m-AAA protease." Kaeser M., Kambacheld M., Kisters-Woike B., Langer T. J. Biol. Chem. 278:46414-46423(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-204; HIS-207 AND HIS-212.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z27116 Genomic DNA. Translation: CAA81638.1. Frameshift. Z28312 Genomic DNA. Translation: CAA82166.1. Frameshift. BK006944 Genomic DNA. Translation: DAA09237.1.
PIR	S38165.
RefSeq	NP_013013.2. NM_001179877.1.
3D structure databases
ProteinModelPortal	P36163.
SMR	P36163. Positions 170-322.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5087N.
MINT	MINT-544282.
STRING	4932.YKR087C.
Protein family/group databases
MEROPS	M48.018.
Proteomic databases
PaxDb	P36163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR087C; YKR087C; YKR087C.
GeneID	853962.
KEGG	sce:YKR087C.
Organism-specific databases
CYGD	YKR087c.
SGD	S000001795. OMA1.
Phylogenomic databases
eggNOG	COG0501.
GeneTree	ENSGT00390000007027.
HOGENOM	HOG000266075.
OMA	PASREME.
OrthoDB	EOG40GH1B.
Gene expression databases
Genevestigator	P36163.
GermOnline	YKR087C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001915. Peptidase_M48. [Graphical view]
Pfam	PF01435. Peptidase_M48. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975389.

Entry information

Entry name

OMA1_YEAST

Accession

Primary (citable) accession number: P36163
Secondary accession number(s): D6VXE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program