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P36162

- DAD2_YEAST

UniProt

P36162 - DAD2_YEAST

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Protein

DASH complex subunit DAD2

Gene

DAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.3 Publications

GO - Biological processi

  1. attachment of spindle microtubules to kinetochore Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. positive regulation of attachment of spindle microtubules to kinetochore Source: SGD
  4. positive regulation of microtubule polymerization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32046-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DASH complex subunit DAD2
Alternative name(s):
DUO1 and DAM1-interacting protein 2
Helper of ASK1 protein 1
Outer kinetochore protein DAD2
Gene namesi
Name:DAD2
Synonyms:HSK1
Ordered Locus Names:YKR083C
ORF Names:YKR403
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR083c.
SGDiS000001791. DAD2.

Subcellular locationi

Nucleus 1 Publication. Cytoplasmcytoskeletonspindle 1 Publication. Chromosomecentromerekinetochore 1 Publication
Note: Associates with the mitotic spindle and the kinetochore.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. DASH complex Source: SGD
  3. microtubule Source: UniProtKB-KW
  4. mitotic spindle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 133133DASH complex subunit DAD2PRO_0000211598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36162.
PaxDbiP36162.

Expressioni

Gene expression databases

GenevestigatoriP36162.

Interactioni

Subunit structurei

The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK1P357348EBI-26515,EBI-26682

Protein-protein interaction databases

BioGridi34214. 53 interactions.
DIPiDIP-1927N.
IntActiP36162. 7 interactions.
MINTiMINT-390119.
STRINGi4932.YKR083C.

Structurei

3D structure databases

ProteinModelPortaliP36162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 5151Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the DASH complex DAD2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG43922.
HOGENOMiHOG000001096.
InParanoidiP36162.
KOiK11567.
OMAiNCRISAS.
OrthoDBiEOG7X3R4R.

Family and domain databases

InterProiIPR013963. DASH_Dad2.
[Graphical view]
PfamiPF08654. DASH_Dad2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36162-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSIDEQIAI KRKELQSLQK ITSLTDGLKI QLTELNEQIK EMGMNADSVA
60 70 80 90 100
QLMNNWDSII NNISQASLGL LQYAEGDYEI GPWKDSKKKE SEQSNETGLE
110 120 130
AQENDKNDED NDEDEDLVPL PETMVRIRVD GNE
Length:133
Mass (Da):15,072
Last modified:June 1, 1994 - v1
Checksum:i82730D4AA5B66951
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z27116 Genomic DNA. Translation: CAA81634.1.
Z28308 Genomic DNA. Translation: CAA82162.1.
AY558397 Genomic DNA. Translation: AAS56723.1.
BK006944 Genomic DNA. Translation: DAA09233.1.
PIRiS38161.
RefSeqiNP_013009.1. NM_001179873.1.

Genome annotation databases

EnsemblFungiiYKR083C; YKR083C; YKR083C.
GeneIDi853958.
KEGGisce:YKR083C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z27116 Genomic DNA. Translation: CAA81634.1 .
Z28308 Genomic DNA. Translation: CAA82162.1 .
AY558397 Genomic DNA. Translation: AAS56723.1 .
BK006944 Genomic DNA. Translation: DAA09233.1 .
PIRi S38161.
RefSeqi NP_013009.1. NM_001179873.1.

3D structure databases

ProteinModelPortali P36162.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34214. 53 interactions.
DIPi DIP-1927N.
IntActi P36162. 7 interactions.
MINTi MINT-390119.
STRINGi 4932.YKR083C.

Proteomic databases

MaxQBi P36162.
PaxDbi P36162.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR083C ; YKR083C ; YKR083C .
GeneIDi 853958.
KEGGi sce:YKR083C.

Organism-specific databases

CYGDi YKR083c.
SGDi S000001791. DAD2.

Phylogenomic databases

eggNOGi NOG43922.
HOGENOMi HOG000001096.
InParanoidi P36162.
KOi K11567.
OMAi NCRISAS.
OrthoDBi EOG7X3R4R.

Enzyme and pathway databases

BioCyci YEAST:G3O-32046-MONOMER.

Miscellaneous databases

NextBioi 975377.

Gene expression databases

Genevestigatori P36162.

Family and domain databases

InterProi IPR013963. DASH_Dad2.
[Graphical view ]
Pfami PF08654. DASH_Dad2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
    Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
    Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The mitotic spindle is required for loading of the DASH complex onto the kinetochore."
    Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J.
    Genes Dev. 16:183-197(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPONENT OF DASH COMPLEX.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex."
    Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G.
    Mol. Cell 17:277-290(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The yeast DASH complex forms closed rings on microtubules."
    Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C.
    Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.
  11. "The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends."
    Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G.
    Nature 440:565-569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Molecular architecture of a kinetochore-microtubule attachment site."
    Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
    Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  13. "The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement."
    Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N.
    Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDAD2_YEAST
AccessioniPrimary (citable) accession number: P36162
Secondary accession number(s): D6VXE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3