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Protein

DASH complex subunit DAD2

Gene

DAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.3 Publications

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32046-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DASH complex subunit DAD2
Alternative name(s):
DUO1 and DAM1-interacting protein 2
Helper of ASK1 protein 1
Outer kinetochore protein DAD2
Gene namesi
Name:DAD2
Synonyms:HSK1
Ordered Locus Names:YKR083C
ORF Names:YKR403
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR083C.
SGDiS000001791. DAD2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002115981 – 133DASH complex subunit DAD2Add BLAST133

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36162.
PRIDEiP36162.

PTM databases

iPTMnetiP36162.

Interactioni

Subunit structurei

The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASK1P357347EBI-26515,EBI-26682

Protein-protein interaction databases

BioGridi34214. 213 interactors.
DIPiDIP-1927N.
IntActiP36162. 7 interactors.
MINTiMINT-390119.
STRINGi4932.YKR083C.

Structurei

3D structure databases

ProteinModelPortaliP36162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 51Sequence analysisAdd BLAST51

Sequence similaritiesi

Belongs to the DASH complex DAD2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000001096.
InParanoidiP36162.
KOiK11567.
OMAiLQYTEND.
OrthoDBiEOG092C59JG.

Family and domain databases

InterProiView protein in InterPro
IPR013963. DASH_Dad2.
PANTHERiPTHR28036. PTHR28036. 1 hit.
PfamiView protein in Pfam
PF08654. DASH_Dad2. 1 hit.

Sequencei

Sequence statusi: Complete.

P36162-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSIDEQIAI KRKELQSLQK ITSLTDGLKI QLTELNEQIK EMGMNADSVA
60 70 80 90 100
QLMNNWDSII NNISQASLGL LQYAEGDYEI GPWKDSKKKE SEQSNETGLE
110 120 130
AQENDKNDED NDEDEDLVPL PETMVRIRVD GNE
Length:133
Mass (Da):15,072
Last modified:June 1, 1994 - v1
Checksum:i82730D4AA5B66951
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27116 Genomic DNA. Translation: CAA81634.1.
Z28308 Genomic DNA. Translation: CAA82162.1.
AY558397 Genomic DNA. Translation: AAS56723.1.
BK006944 Genomic DNA. Translation: DAA09233.1.
PIRiS38161.
RefSeqiNP_013009.1. NM_001179873.1.

Genome annotation databases

EnsemblFungiiYKR083C; YKR083C; YKR083C.
GeneIDi853958.
KEGGisce:YKR083C.

Similar proteinsi

Entry informationi

Entry nameiDAD2_YEAST
AccessioniPrimary (citable) accession number: P36162
Secondary accession number(s): D6VXE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 22, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names