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P36162

- DAD2_YEAST

UniProt

P36162 - DAD2_YEAST

Protein

DASH complex subunit DAD2

Gene

DAD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. positive regulation of attachment of spindle microtubules to kinetochore Source: SGD
    4. positive regulation of microtubule polymerization Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32046-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DASH complex subunit DAD2
    Alternative name(s):
    DUO1 and DAM1-interacting protein 2
    Helper of ASK1 protein 1
    Outer kinetochore protein DAD2
    Gene namesi
    Name:DAD2
    Synonyms:HSK1
    Ordered Locus Names:YKR083C
    ORF Names:YKR403
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR083c.
    SGDiS000001791. DAD2.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmcytoskeletonspindle 1 Publication. Chromosomecentromerekinetochore 1 Publication
    Note: Associates with the mitotic spindle and the kinetochore.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. DASH complex Source: SGD
    3. microtubule Source: UniProtKB-KW
    4. mitotic spindle Source: InterPro

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 133133DASH complex subunit DAD2PRO_0000211598Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP36162.
    PaxDbiP36162.

    Expressioni

    Gene expression databases

    GenevestigatoriP36162.

    Interactioni

    Subunit structurei

    The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ASK1P357348EBI-26515,EBI-26682

    Protein-protein interaction databases

    BioGridi34214. 53 interactions.
    DIPiDIP-1927N.
    IntActiP36162. 7 interactions.
    MINTiMINT-390119.
    STRINGi4932.YKR083C.

    Structurei

    3D structure databases

    ProteinModelPortaliP36162.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1 – 5151Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DASH complex DAD2 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG43922.
    HOGENOMiHOG000001096.
    KOiK11567.
    OMAiNCRISAS.
    OrthoDBiEOG7X3R4R.

    Family and domain databases

    InterProiIPR013963. DASH_Dad2.
    [Graphical view]
    PfamiPF08654. DASH_Dad2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36162-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSIDEQIAI KRKELQSLQK ITSLTDGLKI QLTELNEQIK EMGMNADSVA    50
    QLMNNWDSII NNISQASLGL LQYAEGDYEI GPWKDSKKKE SEQSNETGLE 100
    AQENDKNDED NDEDEDLVPL PETMVRIRVD GNE 133
    Length:133
    Mass (Da):15,072
    Last modified:June 1, 1994 - v1
    Checksum:i82730D4AA5B66951
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z27116 Genomic DNA. Translation: CAA81634.1.
    Z28308 Genomic DNA. Translation: CAA82162.1.
    AY558397 Genomic DNA. Translation: AAS56723.1.
    BK006944 Genomic DNA. Translation: DAA09233.1.
    PIRiS38161.
    RefSeqiNP_013009.1. NM_001179873.1.

    Genome annotation databases

    EnsemblFungiiYKR083C; YKR083C; YKR083C.
    GeneIDi853958.
    KEGGisce:YKR083C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z27116 Genomic DNA. Translation: CAA81634.1 .
    Z28308 Genomic DNA. Translation: CAA82162.1 .
    AY558397 Genomic DNA. Translation: AAS56723.1 .
    BK006944 Genomic DNA. Translation: DAA09233.1 .
    PIRi S38161.
    RefSeqi NP_013009.1. NM_001179873.1.

    3D structure databases

    ProteinModelPortali P36162.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34214. 53 interactions.
    DIPi DIP-1927N.
    IntActi P36162. 7 interactions.
    MINTi MINT-390119.
    STRINGi 4932.YKR083C.

    Proteomic databases

    MaxQBi P36162.
    PaxDbi P36162.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR083C ; YKR083C ; YKR083C .
    GeneIDi 853958.
    KEGGi sce:YKR083C.

    Organism-specific databases

    CYGDi YKR083c.
    SGDi S000001791. DAD2.

    Phylogenomic databases

    eggNOGi NOG43922.
    HOGENOMi HOG000001096.
    KOi K11567.
    OMAi NCRISAS.
    OrthoDBi EOG7X3R4R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32046-MONOMER.

    Miscellaneous databases

    NextBioi 975377.

    Gene expression databases

    Genevestigatori P36162.

    Family and domain databases

    InterProi IPR013963. DASH_Dad2.
    [Graphical view ]
    Pfami PF08654. DASH_Dad2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
      Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
      Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The mitotic spindle is required for loading of the DASH complex onto the kinetochore."
      Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J.
      Genes Dev. 16:183-197(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPONENT OF DASH COMPLEX.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex."
      Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G.
      Mol. Cell 17:277-290(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The yeast DASH complex forms closed rings on microtubules."
      Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C.
      Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.
    11. "The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends."
      Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G.
      Nature 440:565-569(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Molecular architecture of a kinetochore-microtubule attachment site."
      Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
      Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    13. "The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement."
      Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N.
      Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDAD2_YEAST
    AccessioniPrimary (citable) accession number: P36162
    Secondary accession number(s): D6VXE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 279 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3