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P36162 (DAD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DASH complex subunit DAD2
Alternative name(s):
DUO1 and DAM1-interacting protein 2
Helper of ASK1 protein 1
Outer kinetochore protein DAD2
Gene names
Name:DAD2
Synonyms:HSK1
Ordered Locus Names:YKR083C
ORF Names:YKR403
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length133 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. Ref.8 Ref.11 Ref.13

Subunit structure

The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1. Ref.12

Subcellular location

Nucleus. Cytoplasmcytoskeletonspindle. Chromosomecentromerekinetochore. Note: Associates with the mitotic spindle and the kinetochore. Ref.6

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DASH complex DAD2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ASK1P357348EBI-26515,EBI-26682

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 133133DASH complex subunit DAD2
PRO_0000211598

Regions

Coiled coil1 – 5151 Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P36162 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 82730D4AA5B66951

FASTA13315,072
        10         20         30         40         50         60 
MDSIDEQIAI KRKELQSLQK ITSLTDGLKI QLTELNEQIK EMGMNADSVA QLMNNWDSII 

        70         80         90        100        110        120 
NNISQASLGL LQYAEGDYEI GPWKDSKKKE SEQSNETGLE AQENDKNDED NDEDEDLVPL 

       130 
PETMVRIRVD GNE 

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames."
Garcia-Cantalejo J.M., Baladron V., Esteban P.F., Santos M.A., Bou G., Remacha M.A., Revuelta J.L., Ballesta J.P.G., Jimenez A., del Rey F.
Yeast 10:231-245(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The mitotic spindle is required for loading of the DASH complex onto the kinetochore."
Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J.
Genes Dev. 16:183-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPONENT OF DASH COMPLEX.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex."
Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G.
Mol. Cell 17:277-290(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The yeast DASH complex forms closed rings on microtubules."
Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C.
Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.
[11]"The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends."
Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G.
Nature 440:565-569(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Molecular architecture of a kinetochore-microtubule attachment site."
Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[13]"The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement."
Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N.
Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z27116 Genomic DNA. Translation: CAA81634.1.
Z28308 Genomic DNA. Translation: CAA82162.1.
AY558397 Genomic DNA. Translation: AAS56723.1.
BK006944 Genomic DNA. Translation: DAA09233.1.
PIRS38161.
RefSeqNP_013009.1. NM_001179873.1.

3D structure databases

ProteinModelPortalP36162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34214. 53 interactions.
DIPDIP-1927N.
IntActP36162. 7 interactions.
MINTMINT-390119.
STRING4932.YKR083C.

Proteomic databases

MaxQBP36162.
PaxDbP36162.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR083C; YKR083C; YKR083C.
GeneID853958.
KEGGsce:YKR083C.

Organism-specific databases

CYGDYKR083c.
SGDS000001791. DAD2.

Phylogenomic databases

eggNOGNOG43922.
HOGENOMHOG000001096.
KOK11567.
OMANCRISAS.
OrthoDBEOG7X3R4R.

Enzyme and pathway databases

BioCycYEAST:G3O-32046-MONOMER.

Gene expression databases

GenevestigatorP36162.

Family and domain databases

InterProIPR013963. DASH_Dad2.
[Graphical view]
PfamPF08654. DASH_Dad2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975377.

Entry information

Entry nameDAD2_YEAST
AccessionPrimary (citable) accession number: P36162
Secondary accession number(s): D6VXE3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families