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Protein

Nucleoporin NUP133

Gene

NUP133

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP133 is involved in nuclear poly(A)+ RNA, tRNA and pre-ribosome export, in GSP1 nuclear import, in NPC assembly and distribution, as well as in nuclear envelope organization.7 Publications

GO - Molecular functioni

  • nucleocytoplasmic transporter activity Source: GO_Central
  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • double-strand break repair Source: SGD
  • maintenance of chromatin silencing at telomere Source: SGD
  • mRNA export from nucleus in response to heat stress Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nuclear pore distribution Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein import into nucleus Source: SGD
  • telomere tethering at nuclear periphery Source: SGD
  • tRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32045-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP133
Alternative name(s):
Nuclear pore protein NUP133
Gene namesi
Name:NUP133
Synonyms:RAT3
Ordered Locus Names:YKR082W
ORF Names:YKR402
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR082W.
SGDiS000001790. NUP133.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • cytosol Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore outer ring Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002048412 – 1157Nucleoporin NUP133Add BLAST1156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36161.
PRIDEiP36161.

PTM databases

iPTMnetiP36161.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP133 is part of the heptameric 0.5 MDa autoassembling NUP84 NPC subcomplex (NUP84, NUP85, NUP120, NUP133, NUP145C, SEC13 and SEH1).

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP84P528913EBI-11722,EBI-12337

Protein-protein interaction databases

BioGridi34213. 316 interactors.
DIPiDIP-2428N.
IntActiP36161. 12 interactors.
MINTiMINT-525921.

Structurei

Secondary structure

11157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi951 – 965Combined sources15
Helixi973 – 975Combined sources3
Helixi978 – 992Combined sources15
Beta strandi993 – 995Combined sources3
Helixi999 – 1006Combined sources8
Helixi1012 – 1025Combined sources14
Helixi1026 – 1028Combined sources3
Helixi1031 – 1049Combined sources19
Helixi1056 – 1067Combined sources12
Helixi1070 – 1073Combined sources4
Helixi1081 – 1084Combined sources4
Helixi1087 – 1089Combined sources3
Helixi1092 – 1099Combined sources8
Beta strandi1102 – 1104Combined sources3
Helixi1106 – 1123Combined sources18
Helixi1124 – 1127Combined sources4
Helixi1128 – 1141Combined sources14
Beta strandi1144 – 1149Combined sources6
Turni1150 – 1153Combined sources4
Beta strandi1154 – 1157Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KFOX-ray1.90A881-1157[»]
ProteinModelPortaliP36161.
SMRiP36161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36161.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 236Required for normal NPC distributionAdd BLAST193

Domaini

Usually mRNA binding pumilio repeats come in 8 copies. In the 8-fold symmetry of the NPC the 8 repeats may be provided by eight copies of NUP133.

Sequence similaritiesi

Belongs to the nucleoporin Nup133 family.Curated

Phylogenomic databases

HOGENOMiHOG000113873.
InParanoidiP36161.
KOiK14300.
OMAiDQAVYFS.
OrthoDBiEOG092C0EWV.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR014908. Nucleoporin_Nup133/Nup155_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF08801. Nucleoporin_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKKVHLRL RKELSVPIAV VENESLAQLS YEEESQASLM DISMEQQQLR
60 70 80 90 100
LHSHFDNSKV FTENNRYIVK TLQTDYSSGF SNDDELNGYI DMQIGYGLVN
110 120 130 140 150
DHKKVYIWNI HSTQKDTPYI TVPFRSDDND EIAVAPRCIL TFPATMDESP
160 170 180 190 200
LALNPNDQDE TGGLIIIKGS KAIYYEDINS INNLNFKLSE KFSHELELPI
210 220 230 240 250
NSSGGEKCDL MLNCEPAGIV LSTNMGRIFF ITIRNSMGKP QLKLGKLLNK
260 270 280 290 300
PFKLGIWSKI FNTNSSVVSL RNGPILGKGT RLVYITTNKG IFQTWQLSAT
310 320 330 340 350
NSHPTKLIDV NIYEAILESL QDLYPFAHGT LKIWDSHPLQ DESSQLFLSS
360 370 380 390 400
IYDSSCNETY YILSTIIFDS SSNSFTIFST YRLNTFMESI TDTKFKPKIF
410 420 430 440 450
IPQMENANDT NEVTSILVMF PNAVVITQVN SKLDSSYSMR RKWEDIVSLR
460 470 480 490 500
NDIDIIGSGY DSKSLYVLTK QMGVLQFFVK ENEETNSKPE VGFVKSHVDQ
510 520 530 540 550
AVYFSKINAN PIDFNLPPEI SLDQESIEHD LKLTSEEIFH SNGKYIPPML
560 570 580 590 600
NTLGQHLSVR KEFFQNFLTF VAKNFNYKIS PELKLDLIEK FEILNCCIKF
610 620 630 640 650
NSIIRQSDVL NDIWEKTLSN YNLTQNEHLT TKTVVINSPD VFPVIFKQFL
660 670 680 690 700
NHVVFVLFPS QNQNFKLNVT NLINLCFYDG ILEEGEKTIR YELLELDPME
710 720 730 740 750
VDTSKLPWFI NFDYLNCINQ CFFDFTFACE EEGSLDSYKE GLLKIVKILY
760 770 780 790 800
YQFNQFKIWI NTQPVKSVNA NDNFININNL YDDNHLDWNH VLCKVNLKEQ
810 820 830 840 850
CIQIAEFYKD LSGLVQTLQT LDQNDSTTVS LYETFFNEFP KEFSFTLFEY
860 870 880 890 900
LIKHKKLNDL IFRFPQQHDV LIQFFQESAP KYGHVAWIQQ ILDGSYADAM
910 920 930 940 950
NTLKNITVDD SKKGESLSEC ELHLNVAKLS SLLVEKDNLD INTLRKIQYN
960 970 980 990 1000
LDTIDAEKNI SNKLKKGEVQ ICKRFKNGSI REVFNILVEE LKSTTVVNLS
1010 1020 1030 1040 1050
DLVELYSMLD DEESLFIPLR LLSVDGNLLN FEVKKFLNAL VWRRIVLLNA
1060 1070 1080 1090 1100
SNEGDKLLQH IVKRVFDEEL PKNNDFPLPS VDLLCDKSLL TPEYISETYG
1110 1120 1130 1140 1150
RFPIDQNAIR EEIYEEISQV ETLNSDNSLE IKLHSTIGSV AKEKNYTINY

ETNTVEY
Length:1,157
Mass (Da):133,321
Last modified:June 1, 1994 - v1
Checksum:iC8BDBB7D709C5C08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80066 Genomic DNA. Translation: CAA56372.1.
Z27116 Genomic DNA. Translation: CAA81633.1.
Z28307 Genomic DNA. Translation: CAA82161.1.
BK006944 Genomic DNA. Translation: DAA09232.1.
PIRiS38160.
RefSeqiNP_013008.3. NM_001179872.3.

Genome annotation databases

EnsemblFungiiYKR082W; YKR082W; YKR082W.
GeneIDi853957.
KEGGisce:YKR082W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80066 Genomic DNA. Translation: CAA56372.1.
Z27116 Genomic DNA. Translation: CAA81633.1.
Z28307 Genomic DNA. Translation: CAA82161.1.
BK006944 Genomic DNA. Translation: DAA09232.1.
PIRiS38160.
RefSeqiNP_013008.3. NM_001179872.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KFOX-ray1.90A881-1157[»]
ProteinModelPortaliP36161.
SMRiP36161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34213. 316 interactors.
DIPiDIP-2428N.
IntActiP36161. 12 interactors.
MINTiMINT-525921.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP36161.

Proteomic databases

MaxQBiP36161.
PRIDEiP36161.

Protocols and materials databases

DNASUi853957.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR082W; YKR082W; YKR082W.
GeneIDi853957.
KEGGisce:YKR082W.

Organism-specific databases

EuPathDBiFungiDB:YKR082W.
SGDiS000001790. NUP133.

Phylogenomic databases

HOGENOMiHOG000113873.
InParanoidiP36161.
KOiK14300.
OMAiDQAVYFS.
OrthoDBiEOG092C0EWV.

Enzyme and pathway databases

BioCyciYEAST:G3O-32045-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP36161.
PROiP36161.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR014908. Nucleoporin_Nup133/Nup155_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF08801. Nucleoporin_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNU133_YEAST
AccessioniPrimary (citable) accession number: P36161
Secondary accession number(s): D6VXE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.