P36157 (MSA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative transcriptional activator MSA2

Alternative name(s):
MBF and SBF-associated protein 2

Gene names

Name:	MSA2
Ordered Locus Names:	YKR077W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with transcription complexes SCB-binding factor (SBF) and MCB-binding factor (MBF). Interacts with SWI4. Ref.6
Miscellaneous	Present with 861 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription involved in G1 phase of mitotic cell cycle Inferred from physical interaction Ref.6. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Putative transcriptional activator MSA2		PRO_0000203223
Experimental info
	Sequence conflict	122	1	S → R in AAS56248. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P36157 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: D6E5DA379605650A	FASTA	363	39,862
	10 20 30 40 50 60 MVYTTPQQQQ RFSSTPQSSH TLIFSPIRAP SMQTPSSLDY QSPSIVVSSS SMKVHGRSSS 70 80 90 100 110 120 FGKFSLSIGQ NGKATILGPI NVLPTDTSKM EKPVPKKKPV TSDRVEKTRI LSLLKKMRNK 130 140 150 160 170 180 SSTVNKKYSK VPLKSTTSLQ PAATAPSPLV SNIIKPSPKK LASPRTPNAN SNLNLNFTSF 190 200 210 220 230 240 QIKTGFTPNL DGILLENFTS PNTTADSQGN SASNIINNNH GSANNTNQFL FNLPLQSSPR 250 260 270 280 290 300 QFRSPARLID PLPISDWNTS LLMSPPRTTN FESANNHFNS NFAQASMLRR PSLPHIDEVI 310 320 330 340 350 360 PQDSNPANYS DRSDYLSVDQ NANNHNGALS EQTYNNIMKS SMISLPIEKD DATMALRKLV SRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae." Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C. J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH SWI4; SBF AND MBF COMPLEXES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28302 Genomic DNA. Translation: CAA82156.1. AY557922 Genomic DNA. Translation: AAS56248.1. BK006944 Genomic DNA. Translation: DAA09227.1.
PIR	S38154.
RefSeq	NP_013003.1. NM_001179867.1.
3D structure databases
ProteinModelPortal	P36157.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2702N.
IntAct	P36157. 7 interactions.
MINT	MINT-1548078.
STRING	4932.YKR077W.
Proteomic databases
PaxDb	P36157.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR077W; YKR077W; YKR077W.
GeneID	853952.
KEGG	sce:YKR077W.
Organism-specific databases
CYGD	YKR077w.
SGD	S000001785. MSA2.
Phylogenomic databases
eggNOG	NOG12793.
Gene expression databases
Genevestigator	P36157.
GermOnline	YKR077W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	975359.

Entry information

Entry name

MSA2_YEAST

Accession

Primary (citable) accession number: P36157
Secondary accession number(s): D6VXD7, Q6Q5M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program