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Protein

Glutathione S-transferase omega-like 2

Gene

ECM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.1 Publication

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication
A version of this protein truncated after amino acid 200 is not active in the beta-hydroxyethyl disulfide (HED) assay.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication

Kineticsi

  1. KM=1.42 mM for reduced glutathione (GSH)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei46Nucleophile1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • cell wall organization Source: UniProtKB-KW
    • glutathione metabolic process Source: SGD

    Keywordsi

    Molecular functionOxidoreductase, Transferase
    Biological processCell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32040-MONOMER.
    SABIO-RKiP36156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase omega-like 2 (EC:2.5.1.18)
    Alternative name(s):
    Extracellular mutant protein 4
    Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1)
    Gene namesi
    Name:ECM4
    Synonyms:GTO2
    Ordered Locus Names:YKR076W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XI

    Organism-specific databases

    EuPathDBiFungiDB:YKR076W.
    SGDiS000001784. ECM4.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi46C → S or Y: Completely inactive as thiol transferase. No activity recovered against 1-chloro-2,4-dinitrobenzene (CDNB). 1 Publication1
    Mutagenesisi51R → A: No effect on thiol transferase activity. No effect on thiol transferase activity; when associated with D-173. 1 Publication1
    Mutagenesisi173E → A or D: No effect on thiol transferase activity. 1 Publication1
    Mutagenesisi174S → A: No effect on thiol transferase activity. 1 Publication1
    Mutagenesisi246L → A: No effect on thiol transferase activity. 1 Publication1
    Mutagenesisi280G → L: No effect on thiol transferase activity. 1 Publication1
    Mutagenesisi287D → G: Abolishes thiol transferase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000869221 – 370Glutathione S-transferase omega-like 2Add BLAST370

    Proteomic databases

    MaxQBiP36156.
    PRIDEiP36156.

    Expressioni

    Inductioni

    Under oxidative stress conditions. By agents such as diamide, 1-chloro-2,4-dinitrobenzene, tert-butyl hydroperoxide (t-BOOH) and cadmium in a transcriptional factors YAP1 and/or MSN2/4-dependent manner.2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi34207. 259 interactors.
    MINTiMINT-4493799.
    STRINGi4932.YKR076W.

    Structurei

    Secondary structure

    1370
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi37 – 42Combined sources6
    Helixi47 – 58Combined sources12
    Turni62 – 64Combined sources3
    Beta strandi65 – 69Combined sources5
    Helixi95 – 98Combined sources4
    Turni101 – 104Combined sources4
    Helixi106 – 109Combined sources4
    Turni126 – 128Combined sources3
    Turni134 – 136Combined sources3
    Helixi141 – 148Combined sources8
    Beta strandi160 – 163Combined sources4
    Turni164 – 167Combined sources4
    Beta strandi168 – 171Combined sources4
    Helixi174 – 182Combined sources9
    Helixi184 – 189Combined sources6
    Helixi192 – 196Combined sources5
    Turni202 – 204Combined sources3
    Helixi205 – 218Combined sources14
    Turni219 – 221Combined sources3
    Helixi222 – 227Combined sources6
    Helixi232 – 264Combined sources33
    Helixi269 – 273Combined sources5
    Helixi285 – 297Combined sources13
    Turni298 – 300Combined sources3
    Helixi301 – 304Combined sources4
    Helixi312 – 315Combined sources4
    Helixi317 – 329Combined sources13
    Helixi331 – 334Combined sources4
    Helixi339 – 349Combined sources11
    Turni351 – 353Combined sources3
    Beta strandi363 – 367Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5LKBX-ray1.45A/B1-370[»]
    5LKDX-ray1.68A/B1-370[»]
    ProteinModelPortaliP36156.
    SMRiP36156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini201 – 353GST C-terminalAdd BLAST153

    Sequence similaritiesi

    Belongs to the GST superfamily. Omega family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00530000065151.
    HOGENOMiHOG000245143.
    InParanoidiP36156.
    KOiK07393.
    OMAiPWANRAI.
    OrthoDBiEOG092C3FEI.

    Family and domain databases

    InterProiView protein in InterPro
    IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR016639. GST.
    IPR012336. Thioredoxin-like_fold.
    PANTHERiPTHR32419. PTHR32419. 1 hit.
    PfamiView protein in Pfam
    PF13409. GST_N_2. 1 hit.
    PIRSFiPIRSF015753. GST. 1 hit.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 2 hits.
    PROSITEiView protein in PROSITE
    PS50405. GST_CTER. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P36156-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKQWASGTN GAFKRQVSSF RETISKQHPI YKPAKGRYWL YVSLACPWAH
    60 70 80 90 100
    RTLITRALKG LTSVIGCSVV HWHLDEKGWR FLDMEKQLED SEDFLEHWHD
    110 120 130 140 150
    VAGGIRTAKE DSSKSFAEIK NDSQRFMVDA TNEPHYGYKR ISDLYYKSDP
    160 170 180 190 200
    QYSARFTVPV LWDLETQTIV NNESSEIIRI LNSSAFDEFV DDDHKKTDLV
    210 220 230 240 250
    PAQLKTQIDD FNSWVYDSIN NGVYKTGFAE KAEVYESEVN NVFEHLDKVE
    260 270 280 290 300
    KILSDKYSKL KAKYGEEDRQ KILGEFFTVG DQLTEADIRL YTTVIRFDPV
    310 320 330 340 350
    YVQHFKCNFT SIRAGYPFIH LWVRNLYWNY DAFRYTTDFD HIKLHYTRSH
    360 370
    TRINPLGITP LGPKPDIRPL
    Length:370
    Mass (Da):43,274
    Last modified:June 1, 1994 - v1
    Checksum:i2ED6659ADD4CA6ED
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z28301 Genomic DNA. Translation: CAA82155.1.
    BK006944 Genomic DNA. Translation: DAA09226.1.
    PIRiS38153.
    RefSeqiNP_013002.3. NM_001179866.3.

    Genome annotation databases

    EnsemblFungiiYKR076W; YKR076W; YKR076W.
    GeneIDi853951.
    KEGGisce:YKR076W.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiGTO2_YEAST
    AccessioniPrimary (citable) accession number: P36156
    Secondary accession number(s): D6VXD6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: July 5, 2017
    This is version 137 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names