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P36152

- DRE2_YEAST

UniProt

P36152 - DRE2_YEAST

Protein

Fe-S cluster assembly protein DRE2

Gene

DRE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via the FAD- and FMN-containing protein TAH18. Has anti-apoptotic effects in the cell. Involved in negative control of H2O(2)-induced cell death.5 PublicationsUniRule annotation

    Cofactori

    Binds 1 2Fe-2S cluster per subunit. Can also bind a second 4Fe-4S cluster in vitro, but the physiological Fe-S state of DRE2 is still unclear.3 PublicationsUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi252 – 2521Iron-sulfur (2Fe-2S)UniRule annotation
    Metal bindingi263 – 2631Iron-sulfur (2Fe-2S)UniRule annotation
    Metal bindingi266 – 2661Iron-sulfur (2Fe-2S)UniRule annotation
    Metal bindingi268 – 2681Iron-sulfur (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB
    3. electron carrier activity Source: SGD
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. hydrogen peroxide-mediated programmed cell death Source: UniProtKB-HAMAP
    3. iron-sulfur cluster assembly Source: SGD
    4. negative regulation of apoptotic process Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Apoptosis

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32037-MONOMER.
    ReactomeiREACT_188901. Cytosolic iron-sulfur cluster assembly.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fe-S cluster assembly protein DRE2UniRule annotation
    Alternative name(s):
    Anamorsin homologUniRule annotation
    Gene namesi
    Name:DRE2UniRule annotation
    Ordered Locus Names:YKR071C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR071c.
    SGDiS000001779. DRE2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: SGD
    3. mitochondrial intermembrane space Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Fe-S cluster assembly protein DRE2PRO_0000203219Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei206 – 2061Phosphoserine3 Publications

    Post-translational modificationi

    Ubiquitinated.1 PublicationUniRule annotation

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP36152.
    PaxDbiP36152.
    PeptideAtlasiP36152.

    Expressioni

    Gene expression databases

    GenevestigatoriP36152.

    Interactioni

    Subunit structurei

    Monomer By similarity. Interacts with TAH18. Interacts with MIA40.3 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TAH18Q121813EBI-26482,EBI-37624

    Protein-protein interaction databases

    BioGridi34202. 27 interactions.
    DIPiDIP-6716N.
    IntActiP36152. 6 interactions.
    MINTiMINT-672333.
    STRINGi4932.YKR071C.

    Structurei

    Secondary structure

    1
    348
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 117
    Helixi13 – 164
    Helixi19 – 3113
    Beta strandi34 – 418
    Helixi42 – 476
    Beta strandi54 – 563
    Beta strandi59 – 635
    Helixi75 – 828
    Helixi96 – 10510
    Beta strandi107 – 1104
    Beta strandi112 – 1143
    Beta strandi116 – 1194

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KM1NMR-A1-133[»]
    ProteinModelPortaliP36152.
    SMRiP36152. Positions 4-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP36152.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 158158N-terminal domainAdd
    BLAST
    Regioni159 – 24284LinkerAdd
    BLAST
    Regioni243 – 348106Intrinsically disorderedAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi311 – 3144Cx2C motif 1
    Motifi322 – 3254Cx2C motif 2

    Domaini

    The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine.1 PublicationUniRule annotation
    The C-terminal domain binds one 2Fe-2S iron-sulfur cluster but is otherwise mostly in an intrinsically disordered conformation.1 PublicationUniRule annotation
    The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.UniRule annotation

    Sequence similaritiesi

    Belongs to the anamorsin family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG5636.
    GeneTreeiENSGT00390000011417.
    HOGENOMiHOG000209024.
    OMAiTCGLKEI.
    OrthoDBiEOG7C8GWB.

    Family and domain databases

    HAMAPiMF_03115. Anamorsin.
    InterProiIPR007785. Anamorsin.
    [Graphical view]
    PANTHERiPTHR13273. PTHR13273. 1 hit.
    PfamiPF05093. CIAPIN1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36152-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQYKTGLLL IHPAVTTTPE LVENTKAQAA SKKVKFVDQF LINKLNDGSI    50
    TLENAKYETV HYLTPEAQTD IKFPKKLISV LADSLKPNGS LIGLSDIYKV 100
    DALINGFEII NEPDYCWIKM DSSKLNQTVS IPLKKKKTNN TKLQSGSKLP 150
    TFKKASSSTS NLPSFKKADH SRQPIVKETD SFKPPSFKMT TEPKVYRVVD 200
    DLIEDSDDDD FSSDSSKAQY FDQVDTSDDS IEEEELIDED GSGKSMITMI 250
    TCGKSKTKKK KACKDCTCGM KEQEENEIND IRSQQDKVVK FTEDELTEID 300
    FTIDGKKVGG CGSCSLGDAF RCSGCPYLGL PAFKPGQPIN LDSISDDL 348
    Length:348
    Mass (Da):38,543
    Last modified:June 1, 1994 - v1
    Checksum:i8F60A2D097839FB3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28296 Genomic DNA. Translation: CAA82150.1.
    BK006944 Genomic DNA. Translation: DAA09222.1.
    PIRiS38148.
    RefSeqiNP_012997.3. NM_001179861.3.

    Genome annotation databases

    EnsemblFungiiYKR071C; YKR071C; YKR071C.
    GeneIDi853945.
    KEGGisce:YKR071C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28296 Genomic DNA. Translation: CAA82150.1 .
    BK006944 Genomic DNA. Translation: DAA09222.1 .
    PIRi S38148.
    RefSeqi NP_012997.3. NM_001179861.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KM1 NMR - A 1-133 [» ]
    ProteinModelPortali P36152.
    SMRi P36152. Positions 4-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34202. 27 interactions.
    DIPi DIP-6716N.
    IntActi P36152. 6 interactions.
    MINTi MINT-672333.
    STRINGi 4932.YKR071C.

    Proteomic databases

    MaxQBi P36152.
    PaxDbi P36152.
    PeptideAtlasi P36152.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR071C ; YKR071C ; YKR071C .
    GeneIDi 853945.
    KEGGi sce:YKR071C.

    Organism-specific databases

    CYGDi YKR071c.
    SGDi S000001779. DRE2.

    Phylogenomic databases

    eggNOGi COG5636.
    GeneTreei ENSGT00390000011417.
    HOGENOMi HOG000209024.
    OMAi TCGLKEI.
    OrthoDBi EOG7C8GWB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32037-MONOMER.
    Reactomei REACT_188901. Cytosolic iron-sulfur cluster assembly.

    Miscellaneous databases

    EvolutionaryTracei P36152.
    NextBioi 975342.

    Gene expression databases

    Genevestigatori P36152.

    Family and domain databases

    HAMAPi MF_03115. Anamorsin.
    InterProi IPR007785. Anamorsin.
    [Graphical view ]
    PANTHERi PTHR13273. PTHR13273. 1 hit.
    Pfami PF05093. CIAPIN1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae."
      Chanet R., Heude M.
      Curr. Genet. 43:337-350(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
      Tagwerker C., Flick K., Cui M., Guerrero C., Dou Y., Auer B., Baldi P., Huang L., Kaiser P.
      Mol. Cell. Proteomics 5:737-748(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis."
      Zhang Y., Lyver E.R., Nakamaru-Ogiso E., Yoon H., Amutha B., Lee D.W., Bi E., Ohnishi T., Daldal F., Pain D., Dancis A.
      Mol. Cell. Biol. 28:5569-5582(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN FE-S CLUSTER ASSEMBLY, SUBCELLULAR LOCATION, COFACTOR.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast."
      Vernis L., Facca C., Delagoutte E., Soler N., Chanet R., Guiard B., Faye G., Baldacci G.
      PLoS ONE 4:E4376-E4376(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH TAH18.
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesis."
      Netz D.J., Stumpfig M., Dore C., Muhlenhoff U., Pierik A.J., Lill R.
      Nat. Chem. Biol. 6:758-765(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COFACTOR.
    13. "Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-dependent mitochondrial protein trapping machinery."
      Banci L., Bertini I., Ciofi-Baffoni S., Boscaro F., Chatzi A., Mikolajczyk M., Tokatlidis K., Winkelmann J.
      Chem. Biol. 18:794-804(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MIA40.
    14. "Interaction between the reductase Tah18 and highly conserved Fe-S containing Dre2 C-terminus is essential for yeast viability."
      Soler N., Delagoutte E., Miron S., Facca C., Baille D., d'Autreaux B., Craescu G., Frapart Y.M., Mansuy D., Baldacci G., Huang M.E., Vernis L.
      Mol. Microbiol. 82:54-67(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TAH18.
    15. "A S-adenosylmethionine methyltransferase-like domain within the essential, Fe-S-containing yeast protein Dre2."
      Soler N., Craescu C.T., Gallay J., Frapart Y.M., Mansuy D., Raynal B., Baldacci G., Pastore A., Huang M.E., Vernis L.
      FEBS J. 279:2108-2119(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 1-133, DOMAIN, COFACTOR.

    Entry informationi

    Entry nameiDRE2_YEAST
    AccessioniPrimary (citable) accession number: P36152
    Secondary accession number(s): D6VXD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1050 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3