Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P36152

- DRE2_YEAST

UniProt

P36152 - DRE2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Fe-S cluster assembly protein DRE2

Gene

DRE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via the FAD- and FMN-containing protein TAH18. Has anti-apoptotic effects in the cell. Involved in negative control of H2O(2)-induced cell death.5 PublicationsUniRule annotation

Cofactori

[2Fe-2S] cluster3 PublicationsUniRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit. Can also bind a second [4Fe-4S] cluster in vitro, but the physiological Fe-S state of DRE2 is still unclear.3 PublicationsUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi252 – 2521Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi263 – 2631Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi266 – 2661Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi268 – 2681Iron-sulfur (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB
  3. electron carrier activity Source: SGD
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. hydrogen peroxide-mediated programmed cell death Source: UniProtKB-HAMAP
  3. iron-sulfur cluster assembly Source: SGD
  4. negative regulation of apoptotic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32037-MONOMER.
ReactomeiREACT_188901. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Fe-S cluster assembly protein DRE2UniRule annotation
Alternative name(s):
Anamorsin homologUniRule annotation
Gene namesi
Name:DRE2UniRule annotation
Ordered Locus Names:YKR071C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKR071c.
SGDiS000001779. DRE2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: SGD
  3. mitochondrial intermembrane space Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Fe-S cluster assembly protein DRE2PRO_0000203219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei206 – 2061Phosphoserine3 Publications

Post-translational modificationi

Ubiquitinated.1 PublicationUniRule annotation

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP36152.
PaxDbiP36152.
PeptideAtlasiP36152.

Expressioni

Gene expression databases

GenevestigatoriP36152.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with TAH18. Interacts with MIA40.3 PublicationsUniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
TAH18Q121813EBI-26482,EBI-37624

Protein-protein interaction databases

BioGridi34202. 28 interactions.
DIPiDIP-6716N.
IntActiP36152. 6 interactions.
MINTiMINT-672333.
STRINGi4932.YKR071C.

Structurei

Secondary structure

1
348
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117Combined sources
Helixi13 – 164Combined sources
Helixi19 – 3113Combined sources
Beta strandi34 – 418Combined sources
Helixi42 – 476Combined sources
Beta strandi54 – 563Combined sources
Beta strandi59 – 635Combined sources
Helixi75 – 828Combined sources
Helixi96 – 10510Combined sources
Beta strandi107 – 1104Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi116 – 1194Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KM1NMR-A1-133[»]
ProteinModelPortaliP36152.
SMRiP36152. Positions 4-120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36152.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 158158N-terminal domainAdd
BLAST
Regioni159 – 24284LinkerAdd
BLAST
Regioni243 – 348106Intrinsically disorderedAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi311 – 3144Cx2C motif 1
Motifi322 – 3254Cx2C motif 2

Domaini

The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine.1 PublicationUniRule annotation
The C-terminal domain binds one 2Fe-2S iron-sulfur cluster but is otherwise mostly in an intrinsically disordered conformation.1 PublicationUniRule annotation
The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.UniRule annotation

Sequence similaritiesi

Belongs to the anamorsin family.UniRule annotation

Phylogenomic databases

eggNOGiCOG5636.
GeneTreeiENSGT00390000011417.
HOGENOMiHOG000209024.
InParanoidiP36152.
OMAiTCGLKEI.
OrthoDBiEOG7C8GWB.

Family and domain databases

HAMAPiMF_03115. Anamorsin.
InterProiIPR007785. Anamorsin.
[Graphical view]
PANTHERiPTHR13273. PTHR13273. 1 hit.
PfamiPF05093. CIAPIN1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36152-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQYKTGLLL IHPAVTTTPE LVENTKAQAA SKKVKFVDQF LINKLNDGSI
60 70 80 90 100
TLENAKYETV HYLTPEAQTD IKFPKKLISV LADSLKPNGS LIGLSDIYKV
110 120 130 140 150
DALINGFEII NEPDYCWIKM DSSKLNQTVS IPLKKKKTNN TKLQSGSKLP
160 170 180 190 200
TFKKASSSTS NLPSFKKADH SRQPIVKETD SFKPPSFKMT TEPKVYRVVD
210 220 230 240 250
DLIEDSDDDD FSSDSSKAQY FDQVDTSDDS IEEEELIDED GSGKSMITMI
260 270 280 290 300
TCGKSKTKKK KACKDCTCGM KEQEENEIND IRSQQDKVVK FTEDELTEID
310 320 330 340
FTIDGKKVGG CGSCSLGDAF RCSGCPYLGL PAFKPGQPIN LDSISDDL
Length:348
Mass (Da):38,543
Last modified:June 1, 1994 - v1
Checksum:i8F60A2D097839FB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28296 Genomic DNA. Translation: CAA82150.1.
BK006944 Genomic DNA. Translation: DAA09222.1.
PIRiS38148.
RefSeqiNP_012997.3. NM_001179861.3.

Genome annotation databases

EnsemblFungiiYKR071C; YKR071C; YKR071C.
GeneIDi853945.
KEGGisce:YKR071C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28296 Genomic DNA. Translation: CAA82150.1 .
BK006944 Genomic DNA. Translation: DAA09222.1 .
PIRi S38148.
RefSeqi NP_012997.3. NM_001179861.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KM1 NMR - A 1-133 [» ]
ProteinModelPortali P36152.
SMRi P36152. Positions 4-120.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34202. 28 interactions.
DIPi DIP-6716N.
IntActi P36152. 6 interactions.
MINTi MINT-672333.
STRINGi 4932.YKR071C.

Proteomic databases

MaxQBi P36152.
PaxDbi P36152.
PeptideAtlasi P36152.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKR071C ; YKR071C ; YKR071C .
GeneIDi 853945.
KEGGi sce:YKR071C.

Organism-specific databases

CYGDi YKR071c.
SGDi S000001779. DRE2.

Phylogenomic databases

eggNOGi COG5636.
GeneTreei ENSGT00390000011417.
HOGENOMi HOG000209024.
InParanoidi P36152.
OMAi TCGLKEI.
OrthoDBi EOG7C8GWB.

Enzyme and pathway databases

BioCyci YEAST:G3O-32037-MONOMER.
Reactomei REACT_188901. Cytosolic iron-sulfur cluster assembly.

Miscellaneous databases

EvolutionaryTracei P36152.
NextBioi 975342.

Gene expression databases

Genevestigatori P36152.

Family and domain databases

HAMAPi MF_03115. Anamorsin.
InterProi IPR007785. Anamorsin.
[Graphical view ]
PANTHERi PTHR13273. PTHR13273. 1 hit.
Pfami PF05093. CIAPIN1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae."
    Chanet R., Heude M.
    Curr. Genet. 43:337-350(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
    Tagwerker C., Flick K., Cui M., Guerrero C., Dou Y., Auer B., Baldi P., Huang L., Kaiser P.
    Mol. Cell. Proteomics 5:737-748(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis."
    Zhang Y., Lyver E.R., Nakamaru-Ogiso E., Yoon H., Amutha B., Lee D.W., Bi E., Ohnishi T., Daldal F., Pain D., Dancis A.
    Mol. Cell. Biol. 28:5569-5582(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN FE-S CLUSTER ASSEMBLY, SUBCELLULAR LOCATION, COFACTOR.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast."
    Vernis L., Facca C., Delagoutte E., Soler N., Chanet R., Guiard B., Faye G., Baldacci G.
    PLoS ONE 4:E4376-E4376(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH TAH18.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesis."
    Netz D.J., Stumpfig M., Dore C., Muhlenhoff U., Pierik A.J., Lill R.
    Nat. Chem. Biol. 6:758-765(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR.
  13. "Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-dependent mitochondrial protein trapping machinery."
    Banci L., Bertini I., Ciofi-Baffoni S., Boscaro F., Chatzi A., Mikolajczyk M., Tokatlidis K., Winkelmann J.
    Chem. Biol. 18:794-804(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MIA40.
  14. "Interaction between the reductase Tah18 and highly conserved Fe-S containing Dre2 C-terminus is essential for yeast viability."
    Soler N., Delagoutte E., Miron S., Facca C., Baille D., d'Autreaux B., Craescu G., Frapart Y.M., Mansuy D., Baldacci G., Huang M.E., Vernis L.
    Mol. Microbiol. 82:54-67(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TAH18.
  15. "A S-adenosylmethionine methyltransferase-like domain within the essential, Fe-S-containing yeast protein Dre2."
    Soler N., Craescu C.T., Gallay J., Frapart Y.M., Mansuy D., Raynal B., Baldacci G., Pastore A., Huang M.E., Vernis L.
    FEBS J. 279:2108-2119(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-133, DOMAIN, COFACTOR.

Entry informationi

Entry nameiDRE2_YEAST
AccessioniPrimary (citable) accession number: P36152
Secondary accession number(s): D6VXD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3