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P36152 (DRE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fe-S cluster assembly protein DRE2
Alternative name(s):
Anamorsin homolog
Gene names
Name:DRE2
Ordered Locus Names:YKR071C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via the FAD- and FMN-containing protein TAH18. Has anti-apoptotic effects in the cell. Involved in negative control of H2O(2)-induced cell death. Ref.3 Ref.8 Ref.10 Ref.12 Ref.14

Cofactor

Binds 1 2Fe-2S cluster per subunit. Can also bind a second 4Fe-4S cluster in vitro, but the physiological Fe-S state of DRE2 is still unclear. Ref.8 Ref.12 Ref.15

Subunit structure

Monomer By similarity. Interacts with TAH18. Interacts with MIA40. Ref.10 Ref.13 Ref.14

Subcellular location

Cytoplasm. Mitochondrion intermembrane space Ref.4 Ref.8 Ref.13.

Domain

The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine (Ref.15). Ref.15

The C-terminal domain binds one 2Fe-2S iron-sulfur cluster but is otherwise mostly in an intrinsically disordered conformation. Ref.15

The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space By similarity. Ref.15

Post-translational modification

Ubiquitinated. Ref.6

Miscellaneous

Present with 1050 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the anamorsin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TAH18Q121813EBI-26482,EBI-37624

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Fe-S cluster assembly protein DRE2 HAMAP-Rule MF_03115
PRO_0000203219

Regions

Region1 – 158158N-terminal domain HAMAP-Rule MF_03115
Region159 – 24284Linker HAMAP-Rule MF_03115
Region243 – 348106Intrinsically disordered HAMAP-Rule MF_03115
Motif311 – 3144Cx2C motif 1 HAMAP-Rule MF_03115
Motif322 – 3254Cx2C motif 2 HAMAP-Rule MF_03115

Sites

Metal binding2521Iron-sulfur (2Fe-2S) By similarity
Metal binding2631Iron-sulfur (2Fe-2S) By similarity
Metal binding2661Iron-sulfur (2Fe-2S) By similarity
Metal binding2681Iron-sulfur (2Fe-2S) By similarity

Amino acid modifications

Modified residue2061Phosphoserine Ref.7 Ref.9 Ref.11

Secondary structure

........................ 348
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36152 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 8F60A2D097839FB3

FASTA34838,543
        10         20         30         40         50         60 
MSQYKTGLLL IHPAVTTTPE LVENTKAQAA SKKVKFVDQF LINKLNDGSI TLENAKYETV 

        70         80         90        100        110        120 
HYLTPEAQTD IKFPKKLISV LADSLKPNGS LIGLSDIYKV DALINGFEII NEPDYCWIKM 

       130        140        150        160        170        180 
DSSKLNQTVS IPLKKKKTNN TKLQSGSKLP TFKKASSSTS NLPSFKKADH SRQPIVKETD 

       190        200        210        220        230        240 
SFKPPSFKMT TEPKVYRVVD DLIEDSDDDD FSSDSSKAQY FDQVDTSDDS IEEEELIDED 

       250        260        270        280        290        300 
GSGKSMITMI TCGKSKTKKK KACKDCTCGM KEQEENEIND IRSQQDKVVK FTEDELTEID 

       310        320        330        340 
FTIDGKKVGG CGSCSLGDAF RCSGCPYLGL PAFKPGQPIN LDSISDDL 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae."
Chanet R., Heude M.
Curr. Genet. 43:337-350(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C., Flick K., Cui M., Guerrero C., Dou Y., Auer B., Baldi P., Huang L., Kaiser P.
Mol. Cell. Proteomics 5:737-748(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis."
Zhang Y., Lyver E.R., Nakamaru-Ogiso E., Yoon H., Amutha B., Lee D.W., Bi E., Ohnishi T., Daldal F., Pain D., Dancis A.
Mol. Cell. Biol. 28:5569-5582(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN FE-S CLUSTER ASSEMBLY, SUBCELLULAR LOCATION, COFACTOR.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast."
Vernis L., Facca C., Delagoutte E., Soler N., Chanet R., Guiard B., Faye G., Baldacci G.
PLoS ONE 4:E4376-E4376(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH TAH18.
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesis."
Netz D.J., Stumpfig M., Dore C., Muhlenhoff U., Pierik A.J., Lill R.
Nat. Chem. Biol. 6:758-765(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR.
[13]"Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-dependent mitochondrial protein trapping machinery."
Banci L., Bertini I., Ciofi-Baffoni S., Boscaro F., Chatzi A., Mikolajczyk M., Tokatlidis K., Winkelmann J.
Chem. Biol. 18:794-804(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MIA40.
[14]"Interaction between the reductase Tah18 and highly conserved Fe-S containing Dre2 C-terminus is essential for yeast viability."
Soler N., Delagoutte E., Miron S., Facca C., Baille D., d'Autreaux B., Craescu G., Frapart Y.M., Mansuy D., Baldacci G., Huang M.E., Vernis L.
Mol. Microbiol. 82:54-67(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TAH18.
[15]"A S-adenosylmethionine methyltransferase-like domain within the essential, Fe-S-containing yeast protein Dre2."
Soler N., Craescu C.T., Gallay J., Frapart Y.M., Mansuy D., Raynal B., Baldacci G., Pastore A., Huang M.E., Vernis L.
FEBS J. 279:2108-2119(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-133, DOMAIN, COFACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28296 Genomic DNA. Translation: CAA82150.1.
BK006944 Genomic DNA. Translation: DAA09222.1.
PIRS38148.
RefSeqNP_012997.3. NM_001179861.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2KM1NMR-A1-133[»]
ProteinModelPortalP36152.
SMRP36152. Positions 4-120.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34202. 27 interactions.
DIPDIP-6716N.
IntActP36152. 6 interactions.
MINTMINT-672333.
STRING4932.YKR071C.

Proteomic databases

MaxQBP36152.
PaxDbP36152.
PeptideAtlasP36152.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR071C; YKR071C; YKR071C.
GeneID853945.
KEGGsce:YKR071C.

Organism-specific databases

CYGDYKR071c.
SGDS000001779. DRE2.

Phylogenomic databases

eggNOGCOG5636.
GeneTreeENSGT00390000011417.
HOGENOMHOG000209024.
OMATCGLKEI.
OrthoDBEOG7C8GWB.

Enzyme and pathway databases

BioCycYEAST:G3O-32037-MONOMER.
ReactomeREACT_160098. Cytosolic iron-sulfur cluster assembly (yeast).
REACT_191296. Metabolism.

Gene expression databases

GenevestigatorP36152.

Family and domain databases

HAMAPMF_03115. Anamorsin.
InterProIPR007785. Anamorsin.
[Graphical view]
PANTHERPTHR13273. PTHR13273. 1 hit.
PfamPF05093. CIAPIN1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP36152.
NextBio975342.

Entry information

Entry nameDRE2_YEAST
AccessionPrimary (citable) accession number: P36152
Secondary accession number(s): D6VXD2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references