P36152 (DRE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fe-S cluster assembly protein DRE2

Alternative name(s):
Anamorsin homolog

Gene names

Name:	DRE2
Ordered Locus Names:	YKR071C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins By similarity. Has anti-apoptotic effects in the cell. Involved in negative control of H2O(2)-induced cell death, probably by tethering the pro-apoptotic factor TAH18 in the cytoplasm in the absence of oxidative stress By similarity. Ref.3 Ref.9 Ref.11
Cofactor	Binds 4Fe-4S. Binds 2Fe-2S.
Subunit structure	Interacts with TAH18. Ref.11
Subcellular location	Cytoplasm. Mitochondrion intermembrane space Ref.4 Ref.9.
Post-translational modification	Ubiquitinated. Ref.6
Miscellaneous	Present with 1050 molecules/cell in log phase SD medium. HAMAP-Rule MF_03115
Sequence similarities	Belongs to the anamorsin family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Mitochondrion
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster assembly Inferred from mutant phenotype Ref.9PubMed 20802492. Source: SGD
Cellular_component	cytosol Inferred from direct assay Ref.9. Source: SGD mitochondrial intermembrane space Inferred from direct assay Ref.9. Source: SGD
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from direct assay Ref.9. Source: UniProtKB 4 iron, 4 sulfur cluster binding Inferred from direct assay Ref.9. Source: UniProtKB electron carrier activity Inferred from mutant phenotype PubMed 20802492. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TAH18	Q12181	3	EBI-26482,EBI-37624

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 348

348

Fe-S cluster assembly protein DRE2 HAMAP-Rule MF_03115

PRO_0000203219

Regions

Compositional bias

200 – 240

41

Asp/Glu-rich HAMAP-Rule MF_03115

Amino acid modifications

Modified residue

158

1

Phosphoserine Ref.8 Ref.10

Modified residue

186

1

Phosphoserine Ref.8 Ref.10

Modified residue

206

1

Phosphoserine Ref.7 Ref.10

Secondary structure

1

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348

Helix Strand Turn

Details...

Beta strand

5 – 11

7

Helix

13 – 16

4

Helix

19 – 31

13

Beta strand

34 – 41

8

Helix

42 – 47

6

Beta strand

54 – 56

3

Beta strand

59 – 63

5

Helix

75 – 82

8

Helix

96 – 105

10

Beta strand

107 – 110

4

Beta strand

112 – 114

3

Beta strand

116 – 119

4

Sequences

	Sequence		Length	Mass (Da)
	P36152 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 8F60A2D097839FB3	FASTA	348	38,543
	10 20 30 40 50 60 MSQYKTGLLL IHPAVTTTPE LVENTKAQAA SKKVKFVDQF LINKLNDGSI TLENAKYETV 70 80 90 100 110 120 HYLTPEAQTD IKFPKKLISV LADSLKPNGS LIGLSDIYKV DALINGFEII NEPDYCWIKM 130 140 150 160 170 180 DSSKLNQTVS IPLKKKKTNN TKLQSGSKLP TFKKASSSTS NLPSFKKADH SRQPIVKETD 190 200 210 220 230 240 SFKPPSFKMT TEPKVYRVVD DLIEDSDDDD FSSDSSKAQY FDQVDTSDDS IEEEELIDED 250 260 270 280 290 300 GSGKSMITMI TCGKSKTKKK KACKDCTCGM KEQEENEIND IRSQQDKVVK FTEDELTEID 310 320 330 340 FTIDGKKVGG CGSCSLGDAF RCSGCPYLGL PAFKPGQPIN LDSISDDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae." Chanet R., Heude M. Curr. Genet. 43:337-350(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking." Tagwerker C., Flick K., Cui M., Guerrero C., Dou Y., Auer B., Baldi P., Huang L., Kaiser P. Mol. Cell. Proteomics 5:737-748(2006) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, UBIQUITINATION.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158 AND SER-186, MASS SPECTROMETRY.
[9]	"Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis." Zhang Y., Lyver E.R., Nakamaru-Ogiso E., Yoon H., Amutha B., Lee D.W., Bi E., Ohnishi T., Daldal F., Pain D., Dancis A. Mol. Cell. Biol. 28:5569-5582(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN FE-S CLUSTER ASSEMBLY, SUBCELLULAR LOCATION, COFACTOR.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158; SER-186 AND SER-206, MASS SPECTROMETRY.
[11]	"A newly identified essential complex, Dre2-Tah18, controls mitochondria integrity and cell death after oxidative stress in yeast." Vernis L., Facca C., Delagoutte E., Soler N., Chanet R., Guiard B., Faye G., Baldacci G. PLoS ONE 4:E4376-E4376(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN APOPTOSIS, INTERACTION WITH TAH18.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z28296 Genomic DNA. Translation: CAA82150.1.
BK006944 Genomic DNA. Translation: DAA09222.1.

PIR

S38148.

RefSeq

NP_012997.3. NM_001179861.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KM1	NMR	-	A	1-133	[»]

ProteinModelPortal

P36152.

SMR

P36152. Positions 4-120.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6716N.

IntAct

P36152. 6 interactions.

MINT

MINT-672333.

STRING

4932.YKR071C.

Proteomic databases

PaxDb

P36152.

PeptideAtlas

P36152.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKR071C; YKR071C; YKR071C.

GeneID

853945.

KEGG

sce:YKR071C.
sce:YKR074W.

Organism-specific databases

CYGD

YKR071c.

SGD

S000001779. DRE2.

Phylogenomic databases

eggNOG

COG5636.

GeneTree

ENSGT00390000011417.

HOGENOM

HOG000209024.

OMA

TCGLKEI.

OrthoDB

EOG4KPXM9.

Gene expression databases

Genevestigator

P36152.

GermOnline

YKR071C. Saccharomyces cerevisiae.

Family and domain databases

HAMAP

MF_03115. Anamorsin.

InterPro

IPR007785. CIAPIN1.
[Graphical view]

PANTHER

PTHR13273. PTHR13273. 1 hit.

Pfam

PF05093. CIAPIN1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P36152.

NextBio

975342.

Entry information

Entry name

DRE2_YEAST

Accession

Primary (citable) accession number: P36152
Secondary accession number(s): D6VXD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program