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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

MET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Siroheme synthase involved in methionine biosynthesis.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

GO - Molecular functioni

GO - Biological processi

  • methionine biosynthetic process Source: SGD
  • siroheme biosynthetic process Source: SGD
  • sulfate assimilation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32035-MONOMER.
YEAST:G3O-32035-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:MET1
Synonyms:MET20
Ordered Locus Names:YKR069W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKR069w.
EuPathDBiFungiDB:YKR069W.
SGDiS000001777. MET1.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Uroporphyrinogen-III C-methyltransferasePRO_0000150387Add
BLAST

Proteomic databases

MaxQBiP36150.
PaxDbiP36150.
PeptideAtlasiP36150.

Interactioni

Protein-protein interaction databases

BioGridi34200. 9 interactions.
DIPiDIP-4843N.
IntActiP36150. 2 interactions.
MINTiMINT-478835.

Structurei

3D structure databases

ProteinModelPortaliP36150.
SMRiP36150. Positions 301-562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000194244.
InParanoidiP36150.
KOiK00589.
OMAiTHRDVAD.
OrthoDBiEOG7WHHP1.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR016040. NAD(P)-bd_dom.
IPR012066. SUMT_yeast.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PANTHERiPTHR21091:SF96. PTHR21091:SF96. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036555. SUMT_yeast. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRDLVTLPS SLPLITAGFA TDQVHLLIGT GSTDSVSVCK NRIHSILNAG
60 70 80 90 100
GNPIVVNPSS PSHTKQLQLE FGKFAKFEIV EREFRLSDLT TLGRVLVCKV
110 120 130 140 150
VDRVFVDLPI TQSRLCEEIF WQCQKLRIPI NTFHKPEFST FNMIPTWVDP
160 170 180 190 200
KGSGLQISVT TNGNGYILAN RIKRDIISHL PPNISEVVIN MGYLKDRIIN
210 220 230 240 250
EDHKALLEEK YYQTDMSLPG FGYGLDEDGW ESHKFNKLIR EFEMTSREQR
260 270 280 290 300
LKRTRWLSQI MEYYPMNKLS DIKLEDFETS SSPNKKTKQE TVTEGVVPPT
310 320 330 340 350
DENIENGTKQ LQLSEVKKEE GPKKLGKISL VGSGPGSVSM LTIGALQEIK
360 370 380 390 400
SADIILADKL VPQAILDLIP PKTETFIAKK FPGNAERAQQ ELLAKGLESL
410 420 430 440 450
DNGLKVVRLK QGDPYIFGRG GEEFNFFKDH GYIPVVLPGI SSSLACTVLA
460 470 480 490 500
QIPATQRDIA DQVLICTGTG RKGALPIIPE FVESRTTVFL MALHRANVLI
510 520 530 540 550
TGLLKHGWDG DVPAAIVERG SCPDQRVTRT LLKWVPEVVE EIGSRPPGVL
560 570 580 590
VVGKAVNALV EKDLINFDES RKFVIDEGFR EFEVDVDSLF KLY
Length:593
Mass (Da):66,125
Last modified:June 1, 1994 - v1
Checksum:i7C704866B08C0C6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28294 Genomic DNA. Translation: CAA82148.1.
BK006944 Genomic DNA. Translation: DAA09220.1.
PIRiS38145.
RefSeqiNP_012995.3. NM_001179859.3.

Genome annotation databases

EnsemblFungiiYKR069W; YKR069W; YKR069W.
GeneIDi853943.
KEGGisce:YKR069W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28294 Genomic DNA. Translation: CAA82148.1.
BK006944 Genomic DNA. Translation: DAA09220.1.
PIRiS38145.
RefSeqiNP_012995.3. NM_001179859.3.

3D structure databases

ProteinModelPortaliP36150.
SMRiP36150. Positions 301-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34200. 9 interactions.
DIPiDIP-4843N.
IntActiP36150. 2 interactions.
MINTiMINT-478835.

Proteomic databases

MaxQBiP36150.
PaxDbiP36150.
PeptideAtlasiP36150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR069W; YKR069W; YKR069W.
GeneIDi853943.
KEGGisce:YKR069W.

Organism-specific databases

CYGDiYKR069w.
EuPathDBiFungiDB:YKR069W.
SGDiS000001777. MET1.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000194244.
InParanoidiP36150.
KOiK00589.
OMAiTHRDVAD.
OrthoDBiEOG7WHHP1.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32035-MONOMER.
YEAST:G3O-32035-MONOMER.

Miscellaneous databases

NextBioi975336.
PROiP36150.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR016040. NAD(P)-bd_dom.
IPR012066. SUMT_yeast.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PANTHERiPTHR21091:SF96. PTHR21091:SF96. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036555. SUMT_yeast. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes."
    Hansen J., Muldbjerg M., Cherest H., Surdin-Kerjan Y.
    FEBS Lett. 401:20-24(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis."
    Raux E., McVeigh T., Peters S.E., Leustek T., Warren M.J.
    Biochem. J. 338:701-708(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSUMT_YEAST
AccessioniPrimary (citable) accession number: P36150
Secondary accession number(s): D6VXD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 22, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.